Ex-Newcastle winger Darren Ambrose should start, as well as Jonathan Spector, Matt Holland, Talal El Karkouri, Shaun Bartlett, Jonatan Johansson, Jay Bothroyd and ex-Sunderland keeper Thomas Myhre. ...Former Middlesbrough midfielder Phil Stamp has told David Hodgson he is ready to return to the North-East with Darlington. Middlesbrough-born Stamp trained with Quakers yesterday after joining the club on trial with a view to a permanent deal.
JACK Wilkinson this week signed his first professional contract and boss Martin Scott, who handed Michael Maidens his debut and fielded a young bench in last week's win at Blackpool, could pitch the ...striker into his 16 on duty at the weekend. "We've injuries to key players up front and Jack is in there with the rest of them to be involved." Goalkeeper Sam Russell will deliver a timely boost to Darlington's early-season promise when he makes his long-awaited return to the side against Oxford United tomorrow. Former Sunderland midfielder Jonjo Dickman should keep his place in David Hodgson's side, despite taking a knock at Mansfield six days ago, while Matty Appleby will be pushing for a recall after a hip problem.
Darlington boss David Hodgson is not convinced goalkeeper Sam Russell will be fit in time to earn his long-awaited recall at Mansfield Town tomorrow. Hodgson is in no hurry to rush back Russell, and ...has every faith that Frenchman Bert Bossu can continue as a more than able replacement. "If Sam is not fit we continue with Bert, simple as that, " said Hodgson. "The last thing we want to do is bring Sam back a week earlier only to lose him for another month."
CHRIS Llewellyn had a hand in four goals as Wrexham triumphed 6-4 in March, one of the most bizarre games ever seen at Victoria Park. "I watched the play-off games last season with interest and I was ...cheering the lads on. Now our aim is to go one better this season." Craig Hignett will put the disappointment of being overlooked by Darlington manager David Hodgson behind him when he flies out to Cyprus next week. Hignett will fly out to Cyprus on Tuesday after spending the last nine days in Germany, training with AEL ahead of the Cypriot domestic season, which kicks off on August 28.
Both Emre and Scott Parker have not played in any of the games to date and could face Pool as they build up their fitness for next week's second leg with Deportivo. The game kicks off at 7pm tonight ...and with Joel Porter sidelined after a knee operation, Scott could play Michael Proctor or Jon Daly with Adam Boyd in Pool's final game at Victoria Park before Bradford kick-off the new season a week tomorrow. Former Pool defender James Sharp has moved to Torquay, while ex- Pool and Newcastle striker Paul Robinson, who left Victoria Park for York last summer, has signed a two-year deal at Ross County. "He has had a hard time in recent years, but he has a great pedigree and there's plenty of time for him to resurrect his career, starting here, " said boss John Robertson.
DARREN Williams was an unused substitute as Pool eased past a Middlesbrough side at Victoria Park on Tuesday. "He has done well and he's one of a few good young defenders coming through. I've been ...pleased with him." Paul Connor's decision to remain at Swansea City is already a distant memory for Darlington manager David Hodgson, who is already eyeing a move for a Premiership striker. Duke will play some part in Craig Liddle's testimonial game against Middlesbrough tomorrow, while Hodgson will also run the rule over trialists Anthony Peacock and Nathan Jameson.
Pool refused and JACK Ross responded by going on sick leave, a situation that remains and the club has indicated talks won't start until Ross declares himself fit for work. Ross, 28, who has been ...married during the summer, has remained in Scotland while Pool returned for pre-season training and went to Holland to play their first two games. He won't be among Martni Scott's pre-season plans and was omitted from the list when Pool's new squad numbers were announced earlier this week, with Gavin Strachan given his No 12 shirt, but Ross was later added to the list and has now been given squad number 32.
The recently-appointed Hartlepool United boss has made two new signings in recent days, with Cardiff 's Lee Bullock and Wrexham's Chris Llewellyn moving to Victoria Park. Despite recovering from a ...serious knee injury which restricted him to just nine starts last season, Joey Hutchinson is facing more time on the sidelines after suffering a recurrence of a problem first suffered 12 months ago. Former Middlesbrough defender Alan Harrison has resumed full training after a broken leg and will be hoping to earn a permanent deal with Quakers - 12 months after initially joining on trial.
CHRIS Llewellyn was under contract at the Racecourse Ground for another 12 months, but Pool are believed to have paid around 40,000 for his services. "He can play on the left or right, in central ...midfield, off the front man or as a striker - that's five different roles and it's important to have players like that." Martin Scott added: "I spoke to Denis Smith about him and he's gutted to lose him, but Chris sees coming here as improving both his career and his chances of playing for his country. "He can't wait to get started here." Swansea-born Llewellyn moved to north Wales from Norwich in 2003 and has also had a loan spell at Bristol Rovers, facing Pool at the Memorial Ground in March 2003.
Protein interaction networks are crucial for complex cellular processes. However, the elucidation of protein interactions occurring within highly specialised cells and tissues is challenging. Here, ...we describe the development, and application, of a new method for proximity-dependent biotin labelling in whole zebrafish. Using a conditionally stabilised GFP-binding nanobody to target a biotin ligase to GFP-labelled proteins of interest, we show tissue-specific proteomic profiling using existing GFP-tagged transgenic zebrafish lines. We demonstrate the applicability of this approach, termed BLITZ (Biotin Labelling In Tagged Zebrafish), in diverse cell types such as neurons and vascular endothelial cells. We applied this methodology to identify interactors of caveolar coat protein, cavins, in skeletal muscle. Using this system, we defined specific interaction networks within in vivo muscle cells for the closely related but functionally distinct Cavin4 and Cavin1 proteins.
PDF