Most persons infected with enterically transmitted viruses shed large amounts of virus in feces for days or weeks, both before and after onset of symptoms. Therefore, viruses causing gastroenteritis ...may be detected in wastewater, even if only a few persons are infected. In this study, the presence of eight pathogenic viruses (norovirus, astrovirus, rotavirus, adenovirus, Aichi virus, parechovirus, hepatitis A virus HAV, and hepatitis E virus) was investigated in sewage to explore whether their identification could be used as an early warning of outbreaks. Samples of the untreated sewage were collected in proportion to flow at Ryaverket, Gothenburg, Sweden. Daily samples collected during every second week between January and May 2013 were pooled and analyzed for detection of viruses by concentration through adsorption to milk proteins and PCR. The largest amount of noroviruses was detected in sewage 2 to 3 weeks before most patients were diagnosed with this infection in Gothenburg. The other viruses were detected at lower levels. HAV was detected between weeks 5 and 13, and partial sequencing of the structural VP1protein identified three different strains. Two strains were involved in an ongoing outbreak in Scandinavia and were also identified in samples from patients with acute hepatitis A in Gothenburg during spring of 2013. The third strain was unique and was not detected in any patient sample. The method used may thus be a tool to detect incipient outbreaks of these viruses and provide early warning before the causative pathogens have been recognized in health care.
Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment efficiency. In raw sewage human waste is mixed with ...household, industrial and drainage water, and virus particles are, therefore, only found in low concentrations. This necessitates a step of sample concentration to allow for sensitive virus detection. Additionally, viruses harbor a large diversity of both surface and genome structures, which makes universal viral genomic extraction difficult. Current studies have tackled these challenges in many different ways employing a wide range of viral concentration and extraction procedures. However, there is limited knowledge of the efficacy and inherent biases associated with these methods in respect to viral sewage metagenomics, hampering the development of this field. By the use of next generation sequencing this study aimed to evaluate the efficiency of four commonly applied viral concentrations techniques (precipitation with polyethylene glycol, organic flocculation with skim milk, monolithic adsorption filtration and glass wool filtration) and extraction methods (Nucleospin RNA XS, QIAamp Viral RNA Mini Kit, NucliSENS® miniMAG®, or PowerViral® Environmental RNA/DNA Isolation Kit) to determine the viriome in a sewage sample. We found a significant influence of concentration and extraction protocols on the detected viriome. The viral richness was largest in samples extracted with QIAamp Viral RNA Mini Kit or PowerViral® Environmental RNA/DNA Isolation Kit. Highest viral specificity were found in samples concentrated by precipitation with polyethylene glycol or extracted with Nucleospin RNA XS. Detection of viral pathogens depended on the method used. These results contribute to the understanding of method associated biases, within the field of viral sewage metagenomics, making evaluation of the current literature easier and helping with the design of future studies.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
•Land use influenced bacteria in lake inflows, enabling downstream consequences.•Standard fecal indicators are insufficient to detect downstream effects from land use.•Associated with inflows and ...land use, Legionella exemplifies downstream effects.•Reliable access to clean water needs consideration of indirect downstream effects.•This framework can assess new sources in case of drinking water disturbances.
Intensified land use can disturb water quality, potentially increasing the abundance of bacterial pathogens, threatening public access to clean water. This threat involves both direct contamination of faecal bacteria as well as indirect factors, such as disturbed water chemistry and microbiota, which can lead to contamination. While direct contamination has been well described, the impact of indirect factors is less explored, despite the potential of severe downstream consequences on water supply. To assess direct and indirect downstream effects of buildings, farms, pastures and fields on potential water sources, we studied five Swedish lakes and their inflows. We analysed a total of 160 samples in a gradient of anthropogenic activity spanning four time points, including faecal and water-quality indicators. Through species distribution modelling, Random Forest and network analysis using 16S rRNA amplicon sequencing data, our findings highlight that land use indirectly impacts lakes via inflows. Land use impacted approximately one third of inflow microbiota taxa, in turn impacting ∼20–50 % of lake taxa. Indirect effects via inflows were also suggested by causal links between e.g. water colour and lake bacterial taxa, where this influenced the abundance of several freshwater bacteria, such as Polynucleobacter and Limnohabitans. However, it was not possible to identify direct effects on the lakes based on analysis of physiochemical- or microbial parameters. To avoid potential downstream consequences on water supply, it is thus important to consider possible indirect effects from upstream land use and inflows, even when no direct effects can be observed on lakes. Legionella (a genus containing bacterial pathogens) illustrated potential consequences, since the genus was particularly abundant in inflows and was shown to increase by the presence of pastures, fields, and farms. The approach presented here could be used to assess the suitability of lakes as alternative raw water sources or help to mitigate contaminations in important water catchments. Continued broad investigations of stressors on the microbial network can identify indirect effects, avoid enrichment of pathogens, and help secure water accessibility.
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Concentration of viruses in water is necessary for detection and quantification of the viruses present, in order to evaluate microbiological barriers in water treatment plants and detect pathogenic ...viruses during waterborne outbreaks, but there is currently no standardised procedure. In this study, we implemented a previously described fast and simple lanthanum-based protocol for concentration of norovirus genogroup I (GI), genogroup II (GII) and hepatitis A virus (HAV) in drinking and surface water. We compared the results with those of a widely used skimmed milk flocculation method, followed by nucleic acid extraction and RT-qPCR detection. Three seeding levels, with intended concentrations 5 × 10
3
, 5 × 10
4
and 5 × 10
5
genome copies/10 L, were added to drinking water or surface water. All seed levels were detected with both flocculation methods. Samples extracted with skimmed milk flocculation had on average 1.82, 1.86 and 1.38 times higher measured concentration of norovirus GI, GII and HAV, respectively, than those extracted with lanthanum flocculation, across all seeding levels and water types tested. Mengovirus was used as a positive process control. Mengovirus recovery was higher for skimmed milk (40.7% in drinking water, 26.0% in surface water) than for lanthanum flocculation (24.4% in drinking water, 9.7% in surface water). Together, this indicates that skimmed milk flocculation provides higher viral recovery than lanthanum flocculation. However, lanthanum-based flocculation can be performed much faster than skimmed milk flocculation (1.5 h versus 16 h flocculation time) and thus could be a good alternative for rapid monitoring of viruses in water.
One Health surveillance of antimicrobial resistance (AMR) depends on a harmonized method for detection of AMR. Metagenomics-based surveillance offers the possibility to compare resistomes within and ...between different target populations. Its potential to be embedded into policy in the future calls for a timely and integrated knowledge dissemination strategy. We developed a blended training (e-learning and a workshop) on the use of metagenomics in surveillance of pathogens and AMR. The objectives were to highlight the potential of metagenomics in the context of integrated surveillance, to demonstrate its applicability through hands-on training and to raise awareness to bias factors. The target participants included staff of competent authorities responsible for AMR monitoring and academic staff. The training was organized in modules covering the workflow, requirements, benefits and challenges of surveillance by metagenomics. The training had 41 participants. The face-to-face workshop was essential to understand the expectations of the participants about the transition to metagenomics-based surveillance. After revision of the e-learning, we released it as a Massive Open Online Course (MOOC), now available at https://www.coursera.org/learn/metagenomics. This course has run in more than 20 sessions, with more than 3,000 learners enrolled, from more than 120 countries. Blended learning and MOOCs are useful tools to deliver knowledge globally and across disciplines. The released MOOC can be a reference knowledge source for international players in the application of metagenomics in surveillance.
The proinflammatory microenvironment in the respiratory airway induces maturation of both resident and infiltrating dendritic cells (DCs) upon influenza A virus (IAV) infection. This results in ...upregulation of antiviral pathways as well as modulation of endocytic processes, which affect the susceptibility of DCs to IAV infection. Therefore, it is highly relevant to understand how IAV interacts with and infects mature DCs. To investigate how different subsets of human myeloid DCs (MDCs) involved in tissue inflammation are affected by inflammatory stimulation during IAV infection, we stimulated primary blood MDCs and inflammatory monocyte-derived DCs (MDDCs) with TLR ligands, resulting in maturation. Interestingly, MDDCs but not MDCs were protected against IAV infection after LPS (TLR4) stimulation. In contrast, stimulation with TLR7/8 ligand protected MDCs but not MDDCs from IAV infection. The reduced susceptibility to IAV infection correlated with induction of type I IFNs. We found that differential expression of TLR4, TRIF, and MyD88 in the two MDC subsets regulated the ability of the cells to enter an antiviral state upon maturation. This difference was functionally confirmed using small interfering RNA and inhibitors. Our data show that different human MDC subsets may play distinct roles during IAV infection, as their capacity to induce type I IFNs is dependent on TLR-specific maturation, resulting in differential susceptibility to IAV infection.
Globalization and demographic changes are posing new challenges for firms that compete for superior human capital resources, and this ‘war for talent’ is especially fierce among MNCs whose main asset ...is knowledge, like the consultancy industry. As the demand for future leaders increases, it is essential for such firms to ensure an efficient flow of diverse talent through the whole organization. Previous research has mainly treated diversity management and talent management separately, despite the fact that the two concepts are interrelated. This thesis aims to close this gap by exploring how global consultancies incorporate diversity into their talent management practices. By combining in-depth interviews and secondary data from three case companies, conclusions were drawn regarding the views and outcomes of diversity and through which practices the concept was integrated with the management of talents. Some interesting findings were that the main diversity focus was concentrated on gender issues and that global talent management was not highly prioritized in the participating case firms. With regards to talent management, the most highlighted strategy for achieving diversity was by focusing on attracting employees from different diverse groups.
Several recent articles stress that enough research has not been devoted to the performance management of expatriates. The cost of an expatriate is two to three times higher than the cost of a local ...employee. Therefore it is important to ensure that the investment made is returned to the company. Hence, this study aims to investigate how MNC’s evaluate their expatriates. By interviewing expatriate managers in four Swedish MNC’s we came to the conclusion that companies tend to rely much on informal measurements to evaluate expatriates whereas using formal measurements of hard criteria was done in the same way as for all employees. Our findings suggest that companies, in order to get the most out of their expatriates, ought to put more emphasis on the selection process and the repatriation process.
The Weddell Gyre (WG) is one of the main oceanographic features of the Southern Ocean south of the Antarctic Circumpolar Current which plays an influential role in global ocean circulation as well as ...gas exchange with the atmosphere. We review the state‐of‐the art knowledge concerning the WG from an interdisciplinary perspective, uncovering critical aspects needed to understand this system's role in shaping the future evolution of oceanic heat and carbon uptake over the next decades. The main limitations in our knowledge are related to the conditions in this extreme and remote environment, where the polar night, very low air temperatures, and presence of sea ice year‐round hamper field and remotely sensed measurements. We highlight the importance of winter and under‐ice conditions in the southern WG, the role that new technology will play to overcome present‐day sampling limitations, the importance of the WG connectivity to the low‐latitude oceans and atmosphere, and the expected intensification of the WG circulation as the westerly winds intensify. Greater international cooperation is needed to define key sampling locations that can be visited by any research vessel in the region. Existing transects sampled since the 1980s along the Prime Meridian and along an East‐West section at ~62°S should be maintained with regularity to provide answers to the relevant questions. This approach will provide long‐term data to determine trends and will improve representation of processes for regional, Antarctic‐wide, and global modeling efforts—thereby enhancing predictions of the WG in global ocean circulation and climate.
Plain Language Summary
The Weddell Gyre is one of the main oceanographic features in the ocean surrounding Antarctica, the Southern Ocean. Although located far from other continents, this polar region affects the planet through the exchange of gases between frigid ocean waters and the atmosphere, regulating oxygen and carbon dioxide farther north. Studying the Weddell Gyre is challenging, as sea ice covers the ocean surface year around, restricting access by research ships and sensing of ocean surface from satellites. New technology is now available to avoid past limitations, autonomous underwater vehicles, instruments flown by planes, and floats instrumented with sea‐ice detection. Only through international collaboration can we obtain adequate data to populate environmental models and study key areas in the gyre or hot spots. In this review we identify the missing links in our knowledge of the gyre, proposing research to address those questions. Three aspects are critical to understanding the processes that drive the gyre's oceanography, ice, geology, chemistry, and biology: winter and under‐ice conditions that set the stage for the evolution of physics, ice, and biogeochemistry; exchange of water, material, and energy (or heat) with lower latitudes; and intensification of the clockwise circulation of the gyre with changes in winds.
Key Points
Major research priorities to advance understanding of the Weddell Gyre are identified and justified against current knowledge
Interdisciplinary approaches are needed to support system science research of the Weddell Gyre and promote collaborative projects
Winter conditions, connectivity to lower latitudes, and intensification of the gyre are the main interdisciplinary priorities
Understanding changes in Antarctic ice shelf basal melting is a major challenge for predicting future sea level. Currently, warm Circumpolar Deep Water surrounding Antarctica has limited access to ...the Weddell Sea continental shelf; consequently, melt rates at Filchner‐Ronne Ice Shelf are low. However, large‐scale model projections suggest that changes to the Antarctic Slope Front and the coastal circulation may enhance warm inflows within this century. We use a regional high‐resolution ice shelf cavity and ocean circulation model to explore forcing changes that may trigger this regime shift. Our results suggest two necessary conditions for supporting a sustained warm inflow into the Filchner Ice Shelf cavity: (i) an extreme relaxation of the Antarctic Slope Front density gradient and (ii) substantial freshening of the dense shelf water. We also find that the on‐shelf transport over the western Weddell Sea shelf is sensitive to the Filchner Trough overflow characteristics.
Plain Language Summary
The Weddell Sea continental shelf is presently filled with water masses that are too cold to melt the Filchner‐Ronne Ice Shelf from below. If warmer offshore water masses gain access to the Weddell Sea continental shelf and flow into the ice shelf cavity, the ocean‐driven melting would increase rapidly and cause ice shelf thinning. Increased ice shelf melting would supply the continental shelf with freshwater that influence sea ice production and generation of dense water masses and cause an increased discharge of grounded ice, which would contribute to global sea level rise. Two main factors currently prevent warm water from accessing the Weddell Sea continental shelf. First, the warm water is located deeper than the continental shelf and does not have direct access. Second, the Weddell Sea continental shelf is filled with dense water masses that block an inflow of warmer and lighter water. We use a regional ocean model to investigate what would happen if the warm offshore water is lifted higher up or if the dense water masses become fresher and lighter. We find that the Weddell Sea system is robust, and we need to make extreme changes to both factors to allow warm water access to the continental shelf.
Key Points
Thermocline heaving and freshening of the high‐density shelf water are both needed to cause a cold‐to‐warm regime shift in the Weddell Sea
The presence of dense shelf water in Filchner Trough limits inflow of warm water toward the Filchner Ice Shelf front
Reduced Filchner Trough overflow may cause increased on‐shelf transport, and thus warming, in the western Weddell Sea
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