Highlights • Hidradenitis suppurativa (HS) is often a therapeutic challenge. • An effective anti-inflammatory treatment in initial cases is needed. • Zinc gluconate and topical triclosan ...significantly decreased disease severity score (mHSS). • In addition, quality of life (DLQI) was significantly improved. • Combination therapy expands treatment in Hurley stage I and initial stage II.
Highlights • CircularRNAs (circRNAs) have pivotal impacts on the cellular regulatory network. • CircRNAs sequester microRNAs (miRNAs) with miRNA response elements (MREs). • A total of 322 circRNAs ...were identified as differentially expressed in cSCC. • A total of 1603 MREs were identified as part of differentially expressed circRNAs. • Complementary MRE sequence was identified in 23 miRNAs known to be cSCC relevant.
Circular RNAs (circRNAs), are nonprotein coding RNAs consisting of a circular loop with multiple miRNA, binding sites called miRNA response elements (MREs), functioning as miRNA sponges. This study ...was performed to identify differentially expressed circRNAs and their MREs in basal cell carcinoma (BCC).
Microarray circRNA expression profiles were acquired from BCC and control followed by qRT-PCR validation. Bioinformatical target prediction revealed multiple MREs. Sequence analysis was performed concerning MRE interaction potential with the BCC miRNome.
We identified 23 upregulated and 48 downregulated circRNAs with 354 miRNA response elements capable of sequestering miRNA target sequences of the BCC miRNome.
The present study describes a variety of circRNAs that are potentially involved in the molecular pathogenesis of BCC.
Background Data regarding the association of routinely obtained serum markers of inflammation, namely C-reactive protein (CRP), white blood cell count, and neutrophil count, with disease severity of ...hidradenitis suppurativa (HS) according to a scoring system have not been reported to our knowledge. Objective We sought to evaluate these inflammatory serum markers for assessing disease severity of HS. Methods Medical files of 275 patients who were referred to the outpatient HS center of the Department of Dermatology, Venereology, and Allergology, Ruhr-University Bochum, in 2013 were evaluated retrospectively. Results CRP levels and neutrophil count significantly differed among Hurley stages I, II and III ( P < .0001, P = .0002, respectively). There were significant positive correlations among CRP levels ( r = 0.496, P < .0001) and neutrophil count ( r = 0.330, P = .0009) with modified Hidradenitis Suppurativa Score. CRP was a significant independent predictor for Hurley stage III (odds ratio 1.077, 95% confidence interval 1.013-1.145, P = .016). CRP and body mass index were significant independent predictors for severe disease according to modified Hidradenitis Suppurativa Score (odds ratio 1.065, 95% confidence interval 1.015-1.117, P = .009; and odds ratio 1.12, 95% confidence interval 1.009-1.243, P = .032, respectively). Limitations Files were analyzed retrospectively. Conclusion These inflammatory markers, especially CRP, are effective for assessing the extent of disease severity and the grade of inflammation in patients with HS.
Highlights • microRNAs can have an oncogenic or tumor-supressive effect. • miR-17-92 cluster (Oncomir-1) is oncogenic and miR-143-145 tumor supressive. • miR-17-92 and miR-143-5p were highly ...significant increased in cSCC. • miR-145-5p was significantly decreased in BCC. • miR-17-92 and miR-143-145 show dysregulation in epidermal skin cancer.
Highlights • Deregulation of microRNAs may contribute to the pathogenesis in inflammatory diseases. • microRNA key regulators are dysregulated in hidradenitis suppurativa (HS) lesions. • Drosha and ...DGRC8 are likely involved in the initial subclinical inflammation in HS. • Dicer and Exportin-5 may contribute to the later inflammatory process.
Background
Rhinophyma surgery is commonly associated with prolonged wound healing and the need for multiple wound dressings.
Objectives
To evaluate clinical outcome with a porcine extracellular ...matrix (ECM) after shave excision of rhinophyma compared with common wound care procedure.
Materials and methods
Retrospective analysis of patients with common dressings (CD) compared with patients with additional ECM (OASIS) application. Clinical findings were assessed prior to treatment and at follow-up visit using the Patient and Observer Scar Assessment Scale (POSAS), Vancouver Scar Scale (VSS), and Rhinophyma Severity Index (RHISI).
Results
Overall, 28 patients (67.5 ±9.0 years) with a mean wound area of 33.9 (±8.5) cm² were included. After a mean follow-up period of 132 (±73) days, scales of POSAS, VSS, and RHISI showed significant (P< .0001) reductions of 47.0% (±11.1), 56.0% (±12.0), and 62.3% (±14.3), respectively. Subgroup analysis showed no significant differences of aforementioned parameters between the ECM group (n= 17) and CD group (n= 11). In contrast, the number of dressing changes were significantly (P< .006) less in the ECM group (1.4 ±0.8) compared with CD group (4.1 ±2.6). The ECM group showed a significant (P< .017) shorter time to re-epithelization (10.5 ±1.7 days) than the CD group (13.1 ±2.2 days).
Conclusions
The application of porcine ECM is practicable and reduces the number of dressing changes and time to re-epithelization clearly. Crusts are scaling off spontaneously without any aggressive action needed. Our findings indicate that ECM application is a promising approach for rhinophyma wound care.
BACKGROUND/AIMSPerturbations in the expression of microRNAs (miRNAs) and their maturing machinery components such as Dicer have been previously described for basal cell carcinoma (BCC). However, the ...mutational status of Dicer in BCC is unclear. Further, the sclerodermiform subtype of BCC (sBCC) has not been previously investigated regarding its methylation profile or its smallRNA expression profile via RNA sequencing. We conducted this study to investigate the mutational status of Dicer in BCC. METHODSDicer sequencing was performed on the Illumina MiSeq System in a total of 16 BCC samples (8 nodular BCCs, 8 sBCCs) and mapped against the human reference genome (i.e., hg19). Dicer sequencing was performed in all 16 BCC samples. We performed whole genome methylation profiling with Infinium MethylationEPIC BeadChips as well as mRNA and smallRNA sequencing in 5 sBCCs with the Illumina NextSeq500 next-generation sequencing system. RESULTSCompared to the wildtype Dicer sequence, we found 5 to 7 variants per sBCC sample including insertion, deletion, and multiple nucleotide variants. Global methylation profiles were highly similar between groups. mRNA sequencing revealed S100A9, KRT14, KRT10, S100A8, S100A7, COX1, KRT1, COX3, and smallRNA sequencing analysis miR-21, miR-99a, miR26-a-2, let-7f, let-7g, let-7i, miR-100, and miR-205 were the most strongly expressed in sBCCs. CONCLUSIONWe identified a variety of Dicer mutations that could play a role in aberrant miRNA expression in BCC. The noted RNA sequences should be further evaluated in functional studies to explore their potential pathogenetic role in sBCC.
Hidradenitis suppurativa (HS) is a chronic inflammatory disease that commonly develops painful, deep dermal abscesses and chronic, draining sinus tracts. Classically, pharmacologic and surgical ...therapies have been effective for reducing lesion activity and inflammation, but provide only modest success in the prevention of future recurrences and disease progression. Adjunctive therapies, such as laser and light-based therapies, have become more commonly used in the management of HS. These therapies work to reduce the occurrence of painful HS flare-ups by decreasing the number of hair follicles, sebaceous glands, and bacteria in affected areas, and by ablatively debulking chronic lesions. The best results are seen when treatment is individualized, taking disease severity into consideration when selecting specific energy-based approaches. This article will discuss various light-based therapies and the evidence supporting their use in the management of HS.
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