An apprehension for virus contaminant cannot be completely eliminated from urokinase (plasminogen activator) preparation originated from human urine theoretically. In this experiment the effect of ...heat-treatment of urokinase at 60°for 10 hr on infectivity of viruses added intentionally to urokinase samples was investigated. (1) Ten kinds of viruses used were completely inactivated within 5 hr of incubation at 60°. (2) Decrease of urokinase activity due to heat-treatment was avoided by adding albumin as a stabilizer.
Fenestrae were found in freeze-fractured cisternae of the Golgi apparatus and endoplasmic reticulum of glioblastoma, oligodendroglioma, ependymoma, medulloblastoma, medulloepithelioma, meningioma, ...cerebellar sarcoma, hemangioblastoma, and chromophobe adenoma. They were about 200--400 A in diameter and often diffusely distributed or concentrated in groups in Golgi cisternae, while they were around 300--600 A in size and scattered in distribution in cisternae of endoplasmic reticulum. They appeared as conical protrusions or circular broken-off necks of face A and as circular holes on face B in tangential fractures, and as several constrictions of cisternae in cross fractures.
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