Abstract
Most of the existing chest X-ray datasets include labels from a list of findings without specifying their locations on the radiographs. This limits the development of machine learning ...algorithms for the detection and localization of chest abnormalities. In this work, we describe a dataset of more than 100,000 chest X-ray scans that were retrospectively collected from two major hospitals in Vietnam. Out of this raw data, we release 18,000 images that were manually annotated by a total of 17 experienced radiologists with 22 local labels of rectangles surrounding abnormalities and 6 global labels of suspected diseases. The released dataset is divided into a training set of 15,000 and a test set of 3,000. Each scan in the training set was independently labeled by 3 radiologists, while each scan in the test set was labeled by the consensus of 5 radiologists. We designed and built a labeling platform for DICOM images to facilitate these annotation procedures. All images are made publicly available in DICOM format along with the labels of both the training set and the test set.
Late detection of hepatocellular carcinoma (HCC) results in an overall 5-year survival rate of less than 16%. Liquid biopsy (LB) assays based on detecting circulating tumor DNA (ctDNA) might provide ...an opportunity to detect HCC early noninvasively. Increasing evidence indicates that ctDNA detection using mutation-based assays is significantly challenged by the abundance of white blood cell-derived mutations, non-tumor tissue-derived somatic mutations in plasma, and the mutational tumor heterogeneity.
Here, we employed concurrent analysis of cancer-related mutations, and their fragment length profiles to differentiate mutations from different sources. To distinguish persons with HCC (PwHCC) from healthy participants, we built a classification model using three fragmentomic features of ctDNA through deep sequencing of thirteen genes associated with HCC.
Our model achieved an area under the curve (AUC) of 0.88, a sensitivity of 89%, and a specificity of 82% in the discovery cohort consisting of 55 PwHCC and 55 healthy participants. In an independent validation cohort of 54 PwHCC and 53 healthy participants, the established model achieved comparable classification performance with an AUC of 0.86 and yielded a sensitivity and specificity of 81%.
Our study provides a rationale for subsequent clinical evaluation of our assay performance in a large-scale prospective study.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Wastewater sludge is used as an alternative fuel due to its high organic content and calorific value. However, influent characteristics and operational practices of wastewater treatment plants ...(WWTPs) can increase the sulfur content of sludge, devaluing it as a fuel. Thus, we investigated the biochemical mechanisms that elevate the sulfur content of sludge in a full-scale industrial WWTP receiving wastewater of the textile dyeing industry and a domestic WWTP by monitoring the sulfate, sulfur, and iron contents and the biochemical transformation of sulfate to sulfur in the wastewater and sludge treatment streams. A batch sulfate reduction rate test and microbial 16S rRNA and dsrB gene sequencing analyses were applied to assess the potential and activity of sulfate-reducing bacteria and their effect on sulfur deposition. This study indicated that the primary clarifier and anaerobic digester prominently reduced sulfate concentration through biochemical sulfate reduction and iron–sulfur complexation under anaerobic conditions, from 1247 mg/L in the influent to 6.2~59.8 mg/L in the industrial WWTP and from 46.7 mg/L to 0~0.8 mg/L in the domestic WWTPs. The anaerobic sludge, adapted in the high sulfate concentration of the industrial WWTP, exhibited a two times higher specific sulfate reduction rate (0.13 mg SO42−/gVSS/h) and sulfur content (3.14% DS) than the domestic WWTP sludge. Gene sequencing analysis of the population structure of common microbes and sulfate-reducing bacteria indicated the diversity of microorganisms involved in biochemical sulfate reduction in the sulfur cycle, supporting the data revealed by chemical analysis and batch tests.
This study compares the performance of nanosecond pulse (NSP) and direct current (DC) power supplies for use in a municipal wastewater treatment by electrocoagulation (EC). Four Al plates connected ...in monopolar-parallel configuration (MP-P) were used as electrodes during the EC process. The maximum chemical oxygen demand (COD) removal efficiency reached 68% and 80% using DC and NSP, respectively. Moreover, NSP treatment reduced approximately 15% of the specific energy consumption (SEC) compared with that by DC at a similar COD removal efficiency of ≈ 68%, which was used as a benchmark value. In addition, when using NSP, the SEC required to increase the COD removal efficiency from 60% to 68% was two to three times less than that when DC was applied. The results suggest that an NSP operating at 10 kHz frequency (f) and 1 µs pulse width (pw) are preferred for obtaining higher COD removal efficiencies at a low SEC. The use of an NSP for EC can enhance the COD removal efficiency and reduce the wastewater treatment SEC. The results presented herein promote the use of EC systems combined with renewable energy sources for reducing the net carbon footprint of wastewater processing.
Microbial fuel cells (MFCs) show promise in sewage treatment because they can directly convert organic matter (OM) into electricity. This study aimed to demonstrate MFCs stability over 750 days of ...operation and efficient removal of OM and nitrogenous compounds from sewage. To enhance contaminant removal, oxygen was provided into the anode chamber via a mini air pump. This pump was powered by the MFCs' output voltage, which was boosted using a DC-DC converter. The experimental system consisted of 12 sets of cylindrical MFCs within a 246L-scale reactor. The boosted voltage reached 4.7 V. This voltage was first collected in capacitors every 5 min and then dispensed intermittently to the air pump for the MFCs reactor in 4 s. This corresponds to receiving average DO concentration reaching 0.34 ± 0.44 mg/L at 10 cm above the air-stone. Consequently, the degradation rate constants (k) for chemical oxygen demand (COD) and biological oxygen demand (BOD) in the presence of oxygen were 0.048 and 0.069, respectively, which surpassed those without oxygen by 0.039 and 0.044, respectively. Aeration also marginally improved the removal of ammonia because of its potential to create a favorable environment for the growth of anammox and ammonia-oxidizing bacteria such as Candidatus brocadia and Nitrospira. The findings of this study offer in-depth insight into the benefits of boosted voltage in MFCs, highlighting its potential to enhance contaminant degradation. This serves as a foundation for future research focused on improving MFCs performance, particularly for the removal of contaminants from wastewater.
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•MFCs' stability over 750 days of operation without any maintenance.•MFCs' output voltage reached 4.7 V after using a DC-DC converter.•Air pump utilized MFCs' output voltage to provide around 0.34 ± 0.44 mg/L of oxygen in anode chamber.•Degradation rate constants of contaminants in the presence of oxygen were higher than without it.•Presence of oxygen promoted the growth of anammox and ammonia-oxidizing bacteria in the anode chamber.
In this study, the sensory, microbiological, and biochemical qualities were used to examine the quality of Pacific white shrimps (Litopenaeus vannamei) preserved at 0°C during a 10-day period. The ...sensory quality was evaluated by a quality index (QI) that resulted from a quality index method (QIM) scheme. Meanwhile, the total viable count (TVC) and K-value were used to assess the microbiological and biochemical qualities of the shrimp. On day 9, the results from TVC and QIM have shown that the shrimp showed signs of spoilage, corresponding to a log CFU/g of 6.4 and a QI of 21.37, which is unacceptable to consumers. The QI increased linearly with storage days therefore the remaining shelf-life of the shrimp was estimated from a linear regression equation. In particular, this study found a linear relationship between QI, K-value, and hypoxanthine content. Furthermore, hypoxanthine itself could be considered as an independent quality index like the K-index. In conclusion, the quality of Pacific white shrimp was categorized into four different classes: excellent, good, acceptable, and moderately acceptable, based on its sensory and biochemical quality indicators.
In this study, we synthesized and modified titanate nanotubes (TNTs) under different conditions of acid rinsing and calcination. The produced materials were then characterized by transmission ...electron microscopy, X-ray diffraction, ultraviolet-visible spectroscopy, temperature programmed desorption, inductively coupled plasma atomic emission spectroscopy, and Brunauer-Emmett-Teller analysis. The activity of material was evaluated via its application for disinfection of Coliforms and removal of natural organic matters (NOMs) in river water. Results showed that TNTs rinsed at pH 1.6 and calcined at 500°C had the highest removal efficiency for the treatment of Coliforms and NOMs in Sai Gon river water, possibly due to its high surface area, crystallinity, and surface acidity. The application of this TNTs material for the treatment of waters from Dong Nai River and Mekong River also show high removal efficiency, which could meet the quality standard for supply water, suggesting the potential of TNTs for practical drinking water treatment.
•The current findings of the heterogeneous composition of flocs are summarized.•The effect of the composition of flocs on their structure and dynamics is reviewed.•The interaction among heterogeneous ...components within flocs is explained.•Human activities affecting the heterogeneous composition of flocs are also summarized.
Flocculation is a key process for controlling the fate and transport of suspended particulate matter (SPM) in water environments and has received considerable attention in the field of water science (e.g., oceanography, limnology, and hydrology), remaining an active area of research. The research on flocculation has been conducted to elucidate the SPM dynamics and to diagnose various environmental issues. The flocculation, sedimentation, and transportation of SPM are closely linked to the compositional and structural properties of flocs. In fact, flocs are highly heterogeneous in terms of composition. However, the lack of comprehensive research on floc composition and structure has led to misconceptions regarding the temporal and spatial dynamics of SPM. This review summarizes the current understanding of the heterogeneous composition of flocs (e.g., minerals, organic matter, metals, microplastic, engineered nanoparticles) and its effect on their structure and on their fate and transport within aquatic environments. Furthermore, the effects of human activities (e.g., pollutant discharge, construction) on floc composition are discussed.
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This paper proposes the utilization of a nanosecond pulsed (NSP) power supply in the electrocoagulation (EC) system for textile wastewater treatment. Four aluminum plates arranged in a ...monopolar-parallel configuration are utilized as electrodes in a 200 ml EC reactor. Chemical oxygen demand (COD) and specific energy consumption (SEC) are utilized to characterize the performance of NSP-powered EC. A DC power supply is considered as a benchmark. Field-emission scanning electron microscopy, X-ray diffraction, and energy-dispersive spectroscopy are utilized to characterize flocs. The results reveal that a maximum COD removal efficiency of 77% can be achieved by utilizing NSP power, while a value of only 60% can be achieved by utilizing DC power. Additionally, NSP power consumes at least 24% less energy than DC power at a similar COD removal efficiency. The utilization of NSP power for textile wastewater treatment allows for high COD removal efficiency with a significantly lower SEC compared to traditional DC-powered EC. It is believed that the low SEC exhibited by NSP power could be useful for promoting the utilization of EC for wastewater treatment and could contribute to the reduction of the carbon footprint of this process.
•We studied flocculation of microalgae- and clay-containing suspensions.•Investigations were done in different microalgal growth phases.•A two-class flocculation kinetic model and Bayesian ...calibration were applied.•Effect of organic matter secreted by algae on flocculation kinetics was evaluated.•Distinct flocculation mechanisms were identified in different microalgal growth phases.
Interplays between microalgae and clay minerals enhance biologically mediated flocculation, thereby affecting the sedimentation and transportation of suspended particulate matter (SPM) in water and benthic environments. This interaction forms larger flocs with a higher settling velocity and enhances SPM sinking. The aim of this study was to investigate the flocculation kinetics of microalgae and clay in suspension and to elucidate the mechanisms associated with such interactions. Standard jar test experiments were conducted using various mixtures of kaolinite and microalgal samples from batch cultures (Chlorella vulgaris) to estimate biologically mediated flocculation kinetics. The organic matter (OM) composition secreted by the microalgae was characterized using a liquid chromatography - organic carbon detection system, and quantitative analysis of transparent exopolymer particles was conducted separately. A two-class flocculation kinetic model, based on the interaction between flocculi and flocs, was also adopted to quantitatively analyze the experimental data from flocculation. Results from the flocculation kinetic tests and OM analyses, in association with other data analyses (i.e., floc size distribution and flocculation kinetic model), showed that flocculation increased with OM concentration during the growth phase (10–20 d). However, on day 23 during the early stationary phase, flocculation kinetics started decreasing and substantially declined on day 30, even though the amount of OM (mainly biopolymers) continued to increase. Our results indicate that an adequate quantity of biopolymers produced by the microalgal cells in the growth phase enhanced floc-to-floc attachment and hence flocculation kinetics. In contrast, an excessive quantity of biopolymers and humic substances in the stationary phase enhanced the formation of polymeric backbone structures and flocculation via scavenging particles but simultaneously increased steric stabilization with the production of a large number of fragmented particles.
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