Copper oxide nanosheet-loaded zeolite 10X nanocomposites (CuO-zeolite NCs) were successfully prepared by modifying zeolite 10X with CuSO4 aqueous solution. The formation of copper oxide nanosheets on ...the surface of zeolite 10X was observed by SEM. The thickness of CuO nanosheets was about 30–40 nm, and the width ranged from 200 nm to 300 nm. The XRD patterns showed that the new diffraction peaks of copper oxide appeared at 35.6° and 38.8°. According to the XPS results, the Cu 2p3/2 and Cu 2p1/2 peaks in CuO-zeolite NC were centered at 934.1 eV and 953.8 eV, which could be attributed to Cu(II). The EDS analysis revealed that the energy spectra of calcium gradually decreased as the copper ion concentration increased during the preparation of CuO-zeolite NCs. Meanwhile, the energy spectra of copper increased gradually, and the highest content of copper in CuO-zeolite NCs reached 22.35 wt.%. The BET surface areas of zeolite 10X and CuO-zeolite NCs were 587 and 363 m2/g, respectively, based on the N2 adsorption–desorption experiment. The antibacterial activities of CuO-zeolite NC were evaluated using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The antibacterial activities were related to both copper ion content in CuO-zeolite NCs and the particle size of copper oxide. The results showed that nano CuO-loaded zeolite 10X inhibited the activity of E. coli and S. aureus. CuO-zeolite NCs are expected to be further used in antifouling coating.
Objectives
Based on previous reports that ginsenosides have been shown to exert better preventive effects on cisplatin‐induced kidney injury, the present work aims to evaluate the protective effects ...of ginsenoside Rb3 (G‐Rb3) on cisplatin‐induced renal damage and underlying mechanisms in vivo and in vitro.
Materials and methods
The protective effect of G‐Rb3 on cisplatin‐induced acute renal failure in ICR mouse model and HEK293 cell model was investigated, and the underlying possible mechanisms were also explored. For animal experiment, renal function, kidney histology, inflammation, oxidative stress, relative protein molecules involved in apoptosis and autophagy signalling pathways were assessed. In addition, rapamycin (a specific inhibitor of mTOR), compound C (a specific inhibitor of AMPK) and acetylcysteine (NAC, a specific ROS scavenger) were employed to testify the effects of AMPK/mTOR signal pathway on the protective effects of G‐Rb3 in HEK293 cells.
Results
Pre‐treatment with G‐Rb3 at doses of 10 and 20 mg/kg for ten days significantly reversed the increases in serum creatinine (CRE), blood urea nitrogen (BUN) and malondialdehyde (MDA), and decrease in glutathione (GSH) content and superoxide dismutase (SOD) activity. Histopathological examination further revealed that G‐Rb3 inhibited cisplatin‐induced nephrotoxicity. G‐Rb3 diminished cisplatin‐induced increase in protein expression levels of p62, Atg3, Atg5 and Atg7, and decrease in protein expression level of p‐mTOR and the ratio of LC3‐I/LC3‐II, indicating that G‐Rb3 suppressed cisplatin‐induced activation of autophagy. Inhibition of autophagy induced inactivation of apoptosis, which suggested that autophagy played an adverse effect on cisplatin‐evoked renal damage. Further, we found that G‐Rb3 might potentially modulate the expressions of AMPK‐related signal pathways.
Conclusions
These findings clearly suggested that G‐Rb3‐mediated alleviation of cisplatin‐induced nephrotoxicity was in part due to regulation of AMPK‐/mTOR‐mediated autophagy and inhibition of apoptosis in vitro and in vivo.
MADS-box genes are involved in various developmental processes including vegetative development, flower architecture, flowering, pollen formation, seed and fruit development. However, the function of ...most MADS-box genes and their regulation mechanism are still unclear in woody plants compared with model plants. In this study, a MADS-box gene (CiMADS43) was identified in citrus. Phylogenetic and sequence analysis showed that CiMADS43 is a GOA-like Bsister MADS-box gene. It was localized in the nucleus and as a transcriptional activator. Overexpression of CiMADS43 promoted early flowering and leaves curling in transgenic Arabidopsis. Besides, overexpression or knockout of CiMADS43 also showed leaf curl phenotype in citrus similar to that of CiMADS43 overexpressed in Arabidopsis. Protein–protein interaction found that a SEPALLATA (SEP)-like protein (CiAGL9) interacted with CiMADS43 protein. Interestingly, CiAGL9 also can bind to the CiMADS43 promoter and promote its transcription. Expression analysis also showed that these two genes were closely related to seasonal flowering and the development of the leaf in citrus. Our findings revealed the multifunctional roles of CiMADS43 in the vegetative and reproductive development of citrus. These results will facilitate our understanding of the evolution and molecular mechanisms of MADS-box genes in citrus.
Trilobites are one of the most important invertebrate clades in the Palaeozoic, with significant disparity in morphology and behaviour, the latter including intriguing instances of queueing. Previous ...studies employed Computational Fluid Dynamics (CFD) to investigate queuing behaviour in the Devonian trilobite Trimerocephalus chopini and found drag reduction effects. Novel calculations that define a ratio between drag force and Apparent Gravity (W), along with the Submerged Froude Number (Frsub), however, reveal that the obtained drag force was practically negligible in terms of the underwater mobility of trilobites. A trilobite would start to experience difficulty in forward walking only when the relative flow speed was over 42 cm/s, which is inconsistent with the interpreted palaeoenvironment or the predicted moving speed of trilobites. Nevertheless, according to the proposed cantilever model, a trilobite had the ability to sense very minute change in fluid velocity (>7.16 μm/s). High-sensitivity mechanical sensors distributed along the body, either on the exoskeleton or limbs, empowered queuing individuals to discern the fixed self-similar pattern of the wake generated by their predecessors in the queue. In general, if a trilobite were out of the wake, the asymmetrical velocity and pressure field would aid in repositioning itself, facilitating the maintenance of migratory queues. This permitted blind trilobites to securely sense their companions, compensating for lack of long-range visual capability. This paradigm of force assessment is suitable to Computational Fluid Dynamic analyses in other extinct animal-environment interactions, offering a framework to evaluate whether drags and wakes impact more on organism's mobility (W≫0.1,Frsub≫1) or their mechanical sensors, and provides a unique cross-scale insight into animals' adaptation to palaeohydrodynamic variation.
•Criteria to assess relative importance of drag force in terms of mobility and mechanosensors.•Wakes in trilobite queues help them positioning.•Wakes have recognizable self-similarity.•Trilobite can sense 7 μm/s variation in fluid speed through setae.•Drag reduction is only needed for high Reynolds and Froude Numbers.
Yiqi Tongluo Granule (YQTL) is a kind of proprietary Chinese medicine, manufactured by China Shineway Pharmaceutical Group Ltd., under the authority of China Food and Drug Administration (CFDA) ...treating cardiovascular and cerebrovascular diseases such as ischemic stroke in China, however the underlying mechanism of YQTL on treating ischemic stroke has not been revealed. This study is aimed to evaluate the protective effect of YQTL on cerebral ischemia/reperfusion (I/R) injury and inquire into its underlying mechanisms. Cerebral I/R injury was induced by occluding the middle cerebral artery for 2 h followed by 24 h reperfusion. And regional cerebral flow was monitored by Laser Doppler flow during ischemia phase. The infarct volume was evaluated by Triphenyte-trazolium chloride staining. The protective effects of YQTL were assessed by a number of parameters, including neurological scores, regional cerebral blood flow, pathological changes of neuron in hippocampuses and hippocampus calcium level. The proteins of extracellular signal-regulated kinase (ERK), N-methyl D-aspartate receptor subtype 2B (GluN2B) and p-calcium-dependent protein kinaseII (CaMKII) response were assayed by Western blotting. I/R caused significant change in neurological deficit scores, regional cerebral flow and infarct volume. However results in YQTL groups and Nimodipine Tablets (NMDP) group were reversed. Subsequently YQTL reduced I/R-induced calcium influx. Results of hematoxylin–eosin staining manifested that YQTL significantly improved neuronal injury after I/R in rats. Meanwhile, microdialysis data demonstrated that extracellular glutamate was increased in the striatum during ischemia reperfusion, which was reduced by YQTL. YQTL and mitogen-activated protein extracellular kinase (MEK) inhibitor suppressed the I/R-mediated over-expression of GluN2B, p-ERK, ERK and p-CaMKII proteins expression. Putting these together, our results suggest that YQTL played a neuroprotective role in cerebral I/R injury, which might be exerted by inhibiting the excitotoxicity and expression of GluN2B, p-CaMKII and MEK/ERK signal pathway.
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•OGD/R-induced astrocytes damaged and released PRDX6.•The release of PRDX6 aggravated the neuroapoptosis suffering OGD/R.•The mechanism underlying PRDX6 release could be linked to ...aiPLA2 activities.•PRDX6’s pro-apoptotic role in neurons may be associated with RAGE and JNK signaling pathways.
Peroxiredoxin-6 (PRDX6), a member of the peroxiredoxin family, has progressively emerged as a possible therapeutic target for a variety of brain diseases, particularly Alzheimer’s disease and ischemic stroke. However, the role of PRDX6 in neurons under ischemic conditions has remained elusive. Here, we found that astrocytes could release PRDX6 extracellularly after OGD/R, and that PRDX6 release actually worsened neuroapoptosis under OGD/R. We discovered a unique PRDX6/RAGE/JNK signaling pathway that contributes to the effect of neuroapoptosis. We applied a specific inhibitor of the RAGE signaling pathway in a mouse MCAO model and observed significant alterations in animal behavior. Considered together, our findings show the crucial role of the astrocyte-released PRDX6 in the process of neuroapoptosis caused by OGD/R, and could provide novel insights for investigating the molecular mechanism of protecting brain function from ischemia-reperfusion injury.
As high-order nonlinear large-scale systems, wind farms composed of multiple wind turbines (WTs) need to adopt active power control (APC) to track the power set points, rather than the maximum power ...points. In this paper, the proportional distribution strategy is utilized to specify the power set point according to the available output power of each WT based on the ultra-short-term wind speed prediction. Then, we convert the APC problem into the rotor speed tracking control problem, and a robust adaptive fault-tolerant control approach based on the barrier Lyapunov function is developed to track the desired power signal of each WT with guaranteed transient performance and robustness to actuator faults. The effectiveness and the merit of the proposed approach are validated by applying it to the APC of a wind farm.
Considering the superior performances of the future e-p colliders, LHeC and FCC-eh, we discuss the feasibility of detecting the extra neutral scalar
h
2
and the light gauge boson
Z
μ
τ
, which are ...predicted by the
U
(
1
)
L
μ
-
L
τ
model. Taking into account the experimental constraints on the relevant free parameters, we consider all possible production channels of
h
2
and
Z
μ
τ
at e-p colliders and further investigate their observability through the optimal channels in the case of the beam polarization
P
(
e
-
)
=
-
0.8
. We find that the signal significance above
5
σ
of
h
2
as well as
Z
μ
τ
detecting can be achieved via
process and a
5
σ
sensitivity of
Z
μ
τ
detecting can be gained via
process at e-p colliders with appropriate parameter values and a designed integrated luminosity. However, the signals of
h
2
decays into pair of SM particles are difficult to be detected.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The current study investigates how future time perspective (FTP) is linked to career decision-making difficulty among 1,074 Chinese undergraduates. We specifically examined different components of ...FTP (valence and connectedness) as well as their interactions. The results confirmed the following: (1) Valence is positively associated with career decision-making difficulty, whereas connectedness is negatively associated with anxiety and career decision-making difficulty; (2) both career adaptability and anxiety significantly mediate the link from valence/connectedness to career decision-making difficulty, and the serial mediations of valence/connectedness–career adaptability–anxiety–decision-making difficulty are significant; and (3) connectedness moderates the association of valence with career adaptability and career decision-making difficulty but not its association with anxiety. The relationship between valence and career adaptability is higher at a lower level of connectedness, and the relationship between valence and decision-making difficulty is weaker at a higher level of connectedness. Implications for incorporating FTP into adaptability-based career intervention practices are discussed.
MYB family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. To date, few MYB ...genes and little expression profiling have been reported for citrus. Here, we describe and classify 177 members of the sweet orange MYB gene (CsMYB) family in terms of their genomic gene structures and similarity to their putative Arabidopsis orthologs. According to these analyses, these CsMYBs were categorized into four groups (4R-MYB, 3R-MYB, 2R-MYB and 1R-MYB). Gene structure analysis revealed that 1R-MYB genes possess relatively more introns as compared with 2R-MYB genes. Investigation of their chromosomal localizations revealed that these CsMYBs are distributed across nine chromosomes. Sweet orange includes a relatively small number of MYB genes compared with the 198 members in Arabidopsis, presumably due to a paralog reduction related to repetitive sequence insertion into promoter and non-coding transcribed region of the genes. Comparative studies of CsMYBs and Arabidopsis showed that CsMYBs had fewer gene duplication events. Expression analysis revealed that the MYB gene family has a wide expression profile in sweet orange development and plays important roles in development and stress responses. In addition, 337 new putative microsatellites with flanking sequences sufficient for primer design were also identified from the 177 CsMYBs. These results provide a useful reference for the selection of candidate MYB genes for cloning and further functional analysis forcitrus.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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