Mitochondria are involved in ageing and their function requires coordinated action of both mitochondrial and nuclear genes. Epistasis between the two genomes can influence lifespan but whether this ...also holds for reproductive senescence is unclear. Maternal inheritance of mitochondria predicts sex differences in the efficacy of selection on mitonuclear genotypes that should result in differences between females and males in mitochondrial genetic effects. Mitonuclear genotype of a focal individual may also indirectly affect trait expression in the mating partner. We tested these predictions in the seed beetle Callosobruchus maculatus, using introgression lines harbouring distinct mitonuclear genotypes. Our results reveal both direct and indirect sex-specific effects of mitonuclear epistasis on reproductive ageing. Females harbouring coadapted mitonuclear genotypes showed higher lifetime fecundity due to slower senescence relative to novel mitonuclear combinations. We found no evidence for mitonuclear coadaptation in males. Mitonuclear epistasis not only affected age-specific ejaculate weight, but also influenced male age-dependent indirect effects on traits expressed by their female partners (fecundity, egg size, longevity). These results demonstrate important consequences of sex-specific mitonuclear epistasis for both mating partners, consistent with a role for mitonuclear genetic constraints upon sex-specific adaptive evolution.
In dim light, scarcity of photons typically leads to poor vision. Nonetheless, many animals show visually guided behavior with dim environments. We investigated the signaling properties of ...photoreceptors of the dark active cockroach (Periplaneta americana) using intracellular and whole-cell patch-clamp recordings to determine whether they show selective functional adaptations to dark. Expectedly, dark-adapted photoreceptors generated large and slow responses to single photons. However, when light adapted, responses of both phototransduction and the nontransductive membrane to white noise (WN)-modulated stimuli remained slow with corner frequencies ~20 Hz. This promotes temporal integration of light inputs and maintains high sensitivity of vision. Adaptive changes in dynamics were limited to dim conditions. Characteristically, both step and frequency responses stayed effectively unchanged for intensities >1,000 photons/s/photoreceptor. A signal-to-noise ratio (SNR) of the light responses was transiently higher at frequencies <5 Hz for ~5 s after light onset but deteriorated to a lower value upon longer stimulation. Naturalistic light stimuli, as opposed to WN, evoked markedly larger responses with higher SNRs at low frequencies. This allowed realistic estimates of information transfer rates, which saturated at ~100 bits/s at low-light intensities. We found, therefore, selective adaptations beneficial for vision in dim environments in cockroach photoreceptors: large amplitude of single-photon responses, constant high level of temporal integration of light inputs, saturation of response properties at low intensities, and only transiently efficient encoding of light contrasts. The results also suggest that the sources of the large functional variability among different photoreceptors reside mostly in phototransduction processes and not in the properties of the nontransductive membrane.
Low-dose computed tomography tends to produce lower image quality than normal dose computed tomography (CT) although it can help to reduce radiation hazards of CT scanning. Research has shown that ...Artificial Intelligence (AI) technologies, especially deep learning can help enhance the image quality of low-dose CT by denoising images. This scoping review aims to create an overview on how AI technologies, especially deep learning, can be used in dose optimisation for low-dose CT.
Literature searches of ProQuest, PubMed, Cinahl, ScienceDirect, EbscoHost Ebook Collection and Ovid were carried out to find research articles published between the years 2015 and 2020. In addition, manual search was conducted in SweMed+, SwePub, NORA, Taylor & Francis Online and Medic.
Following a systematic search process, the review comprised of 16 articles. Articles were organised according to the effects of the deep learning networks, e.g. image noise reduction, image restoration. Deep learning can be used in multiple ways to facilitate dose optimisation in low-dose CT. Most articles discuss image noise reduction in low-dose CT.
Deep learning can be used in the optimisation of patients’ radiation dose. Nevertheless, the image quality is normally lower in low-dose CT (LDCT) than in regular-dose CT scans because of smaller radiation doses. With the help of deep learning, the image quality can be improved to equate the regular-dose computed tomography image quality.
Lower dose may decrease patients’ radiation risk but may affect the image quality of CT scans. Artificial intelligence technologies can be used to improve image quality in low-dose CT scans. Radiologists and radiographers should have proper education and knowledge about the techniques used.
•Less dose may lower patient radiation risk but may impact image quality of CT scans.•Artificial intelligence technologies can improve image quality in low-dose CT scans.•Different deep learning models have been developed to facilitate dose optimisation in low-dose CT.•Radiologists and radiographers should have proper education and knowledge about the techniques used.
The seed beetle Callosobruchus maculatus is a significant agricultural pest and increasingly studied model of sexual conflict. Males possess genital spines that increase the transfer of seminal fluid ...proteins (SFPs) into the female body. As SFPs alter female behaviour and physiology, they are likely to modulate reproduction and sexual conflict in this species. Here, we identified SFPs using proteomics combined with a de novo transcriptome. A prior 2D‐sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis identified male accessory gland protein spots that were probably transferred to the female at mating. Proteomic analysis of these spots identified 98 proteins, a majority of which were also present within ejaculates collected from females. Standard annotation workflows revealed common functional groups for SFPs, including proteases and metabolic proteins. Transcriptomic analysis found 84 transcripts differentially expressed between the sexes. Notably, genes encoding 15 proteins were highly expressed in male abdomens and only negligibly expressed within females. Most of these sequences corresponded to ‘unknown’ proteins (nine of 15) and may represent rapidly evolving SFPs novel to seed beetles. Our combined analyses highlight 44 proteins for which there is strong evidence that they are SFPs. These results can inform further investigation, to better understand the molecular mechanisms of sexual conflict in seed beetles.
The lack of evolutionary response to selection on mitochondrial genes through males predicts the evolution of nuclear genetic influence on male‐specific mitochondrial function, for example by gene ...duplication and evolution of sex‐specific expression of paralogs involved in metabolic pathways. Intergenomic epistasis may therefore be a prevalent feature of the genetic architecture of male‐specific organismal function. Here, we assess the role of mitonuclear genetic variation for male metabolic phenotypes metabolic rate and respiratory quotient (RQ) associated with ejaculate renewal, in the seed beetle Callosobruchus maculatus, by assaying lines with crossed combinations of distinct mitochondrial haplotypes and nuclear lineages. We found a significant increase in metabolic rate following mating relative to virgin males. Moreover, processes associated with ejaculate renewal showed variation in metabolic rate that was affected by mitonuclear interactions. Mitochondrial haplotype influenced mating‐related changes in RQ, but this pattern varied over time. Mitonuclear genotype and the energy spent during ejaculate production affected the weight of the ejaculate, but the strength of this effect varied across mitochondrial haplotypes showing that the genetic architecture of male‐specific reproductive function is complex. Our findings unveil hitherto underappreciated metabolic costs of mating and ejaculate renewal, and provide the first empirical demonstration of mitonuclear epistasis on male reproductive metabolic processes.
A growing body of research supports the view that within‐species sequence variation in the mitochondrial genome (mtDNA) is functional, in the sense that it has important phenotypic effects. However, ...most of this empirical foundation is based on comparisons across populations, and few studies have addressed the functional significance of mtDNA polymorphism within populations. Here, using mitonuclear introgression lines, we assess differences in whole‐organism metabolic rate of adult Drosophila subobscura fruit flies carrying either of three different sympatric mtDNA haplotypes. We document sizeable, up to 20%, differences in metabolic rate across these mtDNA haplotypes. Further, these mtDNA effects are to some extent sex specific. We found no significant nuclear or mitonuclear genetic effects on metabolic rate, consistent with a low degree of linkage disequilibrium between mitochondrial and nuclear genes within populations. The fact that mtDNA haplotype variation within a natural population affects metabolic rate, which is a key physiological trait with important effects on life‐history traits, adds weight to the emergent view that mtDNA haplotype variation is under natural selection and it revitalizes the question as to what processes act to maintain functional mtDNA polymorphism within populations.
In this note, we consider output regulation and disturbance rejection of periodic signals via state feedback in the setting of exponentially stabilizable linear infinite-dimensional systems. We show ...that if an infinite-dimensional exogenous system is generating periodic reference signals, solvability of the state feedback regulation problem is equivalent to solvability of the so called equations. This result allows us to consider asymptotic tracking of periodic reference signals which only have absolutely summable Fourier coefficients, while in related existing work the reference signals are confined to be infinitely smooth. We also discuss solution of the regulator equations and construct the actual feedback law to achieve output regulation in the single-input-single-output (SISO) case: The output regulation problem is solvable if the transfer function of the stabilized plant does not have zeros at the frequencies i/spl omega//sub n/ of the periodic reference signals and if the sequence (CR(i/spl omega//sub n/, A+BK)B/sup -1/ /spl times/(Q/spl phi//sub n/-CR(i/spl omega//sub n/, A+BK)P/spl phi//sub n/)) /sub n/spl isin/z//spl isin/l/sup n2/. A one-dimensional heat equation is used as an illustrative example.
By using a delay line as a signal generator, we design a simple robust controller for the approximate regulation (i.e., approximate asymptotic tracking and rejection) of almost periodic signals for ...linear systems. As opposed to the related existing results, our controller design does not require online parameter tuning. Besides the usual assumptions about stabilizability, detectability, and nonexistence of transmission zeros for the plant, the controller design algorithm of this paper only requires knowledge of the desired tracking accuracy . Based on this information alone, we can only design a robust controller achieving, in the presence of unknown trigonometric polynomial disturbances and additive perturbations to the plant and the controller, . Here, is the output of the plant and is an arbitrary almost periodic reference signal taken from an infinite-dimensional generalized Sobolev space of almost periodic functions. In this paper, we also study robust output regulation in the limiting case where . It turns out that, due to the general loss of exponential closed loop stability, asymptotic tracking/rejection can also be lost as , unless the exogenous signals are smooth enough. This last result extends some recent theorems on (nonrobust) open loop output regulation for infinite-dimensional exosystems. The results of this paper are new and potentially useful for finite-dimensional plants, but the main results are also true for infinite-dimensional systems with bounded control and observation operators.
To compare the effects of the food toxin 2-amino-1-methyl-6-phenyl-imidazo4,5-bpyridine (PhIP) and estradiol in hormone-responsive MCF-7 cells, the cells were exposed to different concentrations of ...either PhIP or estradiol. The effect of various culture conditions (e.g. phenol red, FBS, vehicle (DMSO/EtOH) and seeding density) on responses was studied. Cells were continuously grown with steroid-containing or -deprived medium, or switched from steroid-containing to -deprived medium for the experiments to minimize the effect of background estrogenicity. Effects of PhIP and estradiol on cell viability and proliferation were determined by ATP analysis and Ki-67 immunocytochemistry. Expression of estrogen receptor alpha, cell stress markers (p53 and ERK) and estrogen responsive proteins (c-myc and ERK) were immunoblotted. All concentrations of estradiol induced cell proliferation, viability and changes in protein expression, typical for estrogenic responses. PhIP, however, increased viability only at low concentrations and depending on culture conditions. No changes in protein expressions by PhIP were noted, not even when switching cells from steroid-containing to -deprived medium which down-regulated the expression of proteins at basal level. Vehicle affected significantly viability, especially after exposure to PhIP, but not protein expression while medium changes affected both. In conclusion, the effects of PhIP and estradiol in MCF-7 cells are dependent on culture conditions. The detected PhIP-induced changes are weaker compared to those induced by estradiol.
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