Agrobacterium rhizogenes (or Rhizobium rhizogenes) is able to transform plant genomes and induce the production of hairy roots. We describe the use of A. rhizogenes in tomato (Solanum spp.) to ...rapidly assess gene expression and function. Gene expression of reporters is indistinguishable in plants transformed by Agrobacterium tumefaciens as compared with A. rhizogenes. A root cell type- and tissue-specific promoter resource has been generated for domesticated and wild tomato (Solanum lycopersicum and Solanum pennellii, respectively) using these approaches. Imaging of tomato roots using A. rhizogenes coupled with laser scanning confocal microscopy is facilitated by the use of a membrane-tagged protein fused to a red fluorescent protein marker present in binary vectors. Tomato-optimized isolation of nuclei tagged in specific cell types and translating ribosome affinity purification binary vectors were generated and used to monitor associated messenger RNA abundance or chromatin modification. Finally, transcriptional reporters, translational reporters, and clustered regularly interspaced short palindromic repeats-associated nuclease9 genome editing demonstrate that SHORT-ROOT and SCARECROW gene function is conserved between Arabidopsis (Arabidopsis thaliana) and tomato.
In this Letter, we report analyses of spatiotemporal dynamics of turbulence and structure in the limit-cycle oscillation (LCO) that precedes an L-to-H transition. Zonal flows are not observed during ...LCO, and the oscillation is the periodic generations or decays of barrier with edge-localized mean flow. Oscillatory Reynolds stress is found to be too small to accelerate the LCO flow, by considering the dielectric constant in magnetized toroidal plasmas. Propagation of changes of the density gradient and turbulence amplitude into the core is also observed.
The variety of scalar and vector fields in laboratory and nature plasmas is formed by plasma turbulence. Drift-wave fluctuations, driven by density gradients in magnetized plasmas, are known to relax ...the density gradient while they can generate flows. On the other hand, the sheared flow in the direction of magnetic fields causes Kelvin-Helmholtz type instabilities, which mix particle and momentum. These different types of fluctuations coexist in laboratory and nature, so that the multiple mechanisms for structural formation exist in extremely non-equilibrium plasmas. Here we report the discovery of a new order in plasma turbulence, in which chained structure formation is realized by cross-interaction between inhomogeneities of scalar and vector fields. The concept of cross-ferroic turbulence is developed, and the causal relation in the multiple mechanisms behind structural formation is identified, by measuring the relaxation rate and dissipation power caused by the complex turbulence-driven flux.
Abstract
The velocity distribution function is a statistical description that connects particle kinetics and macroscopic parameters in many-body systems. Laser-induced fluorescence (LIF) spectroscopy ...is utilized to measure the local velocity distribution function in spatially inhomogeneous plasmas. However, the analytic form of such a function for the system of interest is not always clear under the intricate factors in non-equilibrium states. Here, we propose a novel approach to select the valid form of the velocity distribution function based on Bayesian statistics. We formulate the Bayesian inference of ion velocity distribution function and apply it to LIF spectra locally observed at several positions in a linear magnetized plasma. We demonstrate evaluating the spatial inhomogeneity by verifying each analytic form of the local velocity distribution function. Our approach is widely applicable to experimentally establish the velocity distribution function in plasmas and fluids, including gases and liquids.
Abstract
This article discusses triad transfer analysis via quadratic nonlinearity. To avoid fictitious interactions, symmetrization of the triad transfer is reviewed, including arbitrary orthogonal ...decomposition and coarse graining. The directional representation of the symmetrized triad transfer is proposed by minimizing the number of edges in a network graph of triad interactions with keeping the energy consistency. The directional representation simplifies visualization and allows us to reduce the energy transfer into a one-to-one relation, while eliminating fictitious interactions in non-symmetrized triad transfer functions. Energy transfer processes among plasma turbulent fluctuations that decompose by the singular value decomposition are analyzed as an application. A network graph visualization clearly demonstrates the importance of symmetrization and the consistency between the symmetrized triad transfer and its directional representation.
The novel technique of dynamical mode decomposition (DMD) is applied to the outputs of a numerical simulation of Kelvin-Helmholtz turbulence in a cylindical plasma, so as to capture and quantify the ...time evolution of the dominant nonlinear structures. Empirically, these structures comprise rotationally symmetric deformations together with spiral patterns, and they are found to be identified as the main modes of the DMD. A new method to calculate the time evolution of DMD mode amplitudes is proposed, based on convolution-type correlation integrals, and then applied to the simulation outputs in a limit cycle regime. The resulting time traces capture the essential physics far better than Fourier techniques applied to the same data.
Abstract
A new passive monitoring system applied to large surface wave plasmas (SWP) is experimentally tested. In the first experiment, spatial distributions of the electron density and temperature ...are measured using double probes. Subsequently, integral photography with deconvolution techniques is applied to identify the three-dimensional emissivity distribution of SWP. The result obtained from the first experiment seems to identify the localization of SWP around the antenna launching an 860 MHz microwave.
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