A model has been developed which allows an isolated lung to be perfused by a blood-PSS solution continuously processed by an intact animal. This model combines the advantage of control of the ...isolated perfused lung with a recirculated perfusate that is physiologically maintained. Substances that are generated by the isolated lung are, therefore, removed by metabolism in the intact animal and essential consumables in the perfusate are restored. Studies were performed in rabbits utilizing isolated perfused lung both with and without the intact animal processing the perfusate. The greatest pressor responses to hypoxic ventilation are obtained with a non-processed recirculated perfusate circuit, but the pressor responses decrease with time (12.5 +/- 1.7 cmH2O to 8.2 +/- 1.3 cmH3O) and the baseline pressure is higher initially (18.5 +/- 0.7 mmH2O). With the whole animal in the circuit, the baseline pressure and the initial hypoxic pressor responses are not as large, but are maintained constant (6.8 +/- 0.5 cmH2O to 8.1 +/- 1.0 cmH2O) over the same time period. When the isolated lungs are perfused with either blood-PSS or PSS in a non-processed, non-recirculated system, the hypoxic responses are 8.7 +/- 1.7 cmH2O and 5.7 +/- 1.0 cmH2O at the beginning of the study and decrease with time to 6.5 +/- 1.3 cmH2O and 4.2 +/- 1.0 cmH2O, respectively. The response of the isolated lung is, therefore, more stable with time and shows less individual variability when the whole animal perfusion circuit is employed.
Using monospecific antibody to human urinary trypsin inhibitor, we developed a highly specific and sensitive radioimmunoassay (RIA) for measuring human urinary trypsin inhibitor. No cross-reactivity ...of the antibody with protein standard serum, which contained albumin, alpha 1-antitrypsin, haptoglobin, alpha 2-macroglobulin, transferrin, IgG and IgA, was observed. The sensitivity of the system was 10 ng of trypsin inhibitor per assay tube, and 5-10 microliters of urine was sufficient to determine the concentration of trypsin inhibitor in urine. The amounts excreted in the urine of 10 healthy men and 10 healthy women were 4.83 +/- 2.46 (mean +/- SD) and 3.86 +/- 1.35 mg/day, respectively. The correlation between estimates by RIA and those by enzymic assay was r = 0.96 (p less than 0.005). The method proposed here can be used to determine the concentration of urinary trypsin inhibitor in a small amount of biological fluids and cells.
A single radial immunodiffusion method is described for determining the serum protein which reacts with antibody against the urinary trypsin inhibitor, but does not react with ...anti-inter-alpha-trypsin inhibitor antibody. Since the amount of this protein could not be determined with an agar plate containing antibody alone, we first prepared an agar plate containing 4% polyethylene glycol 6 000 and 1 mg of anti-urinary trypsin inhibitor gamma-globulin and confirmed that the amount of this protein could be measured accurately from the sizes of precipitin rings after 72 h incubation at room temperature. Levels of this serum protein, alpha 1-antitrypsin, alpha 2-macroglobulin and inter-alpha-trypsin inhibitor were measured in normal human serum by the single radial immunodiffusion method, and it was confirmed that the level of this serum protein did not correlate with the levels of these 3 other proteins.
The 30th case of metastatic skin carcinoma of prostatic origin was presented. The cytosolic R1881 receptor was detected moderately in the metastatic nodule of the skin, however, the measurement of ...this receptor had a limited value for the purpose of treating the patient with antiandrogenic agents.
The sensitivity of a renal neoplasm induced in vivo with C type RNA virus to anticancer drugs was evaluated. The proliferation rate of the virus induced neoplasm cells depended on the concentrations ...of antimetabolites, alkylates and antibiotics, but not depends on that of alkaloids. An inhibitory effect of drugs on the virus induced neoplasm cells became evident in the following order, i.e., antimetabolites, alkylates, antibiotics and then alkaloids.
Asynchronous transfer mode (ATM) transmission is used in the broadband integrated services digital network (B-ISDN). Various terminals, such as telephones, telefaxes, pay-TVs, and workstations will ...be connected together through the B-ISDN. Fixed-length (53 byte) short packets, called "cells" are used for all data transmission.
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