This paper reports a multi-throughput multi-organ-on-a-chip system formed on a pneumatic pressure-driven medium circulation platform with a microplate-sized format as a novel type of ...microphysiological system. The pneumatic pressure-driven platform enabled parallelized multi-organ experiments (i.e. simultaneous operation of multiple multi-organ culture units) and pipette-friendly liquid handling for various conventional cell culture experiments, including cell seeding, medium change, live/dead staining, cell growth analysis, gene expression analysis of collected cells, and liquid chromatography-mass spectrometry analysis of chemical compounds in the culture medium. An eight-throughput two-organ system and a four-throughput four-organ system were constructed on a common platform, with different microfluidic plates. The two-organ system, composed of liver and cancer models, was used to demonstrate the effect of an anticancer prodrug, capecitabine (CAP), whose metabolite 5-fluorouracil (5-FU) after metabolism by HepaRG hepatic cells inhibited the proliferation of HCT-116 cancer cells. The four-organ system, composed of intestine, liver, cancer, and connective tissue models, was used to demonstrate evaluation of the effects of 5-FU and two prodrugs of 5-FU (CAP and tegafur) on multiple organ models, including cancer and connective tissue.
Aims/hypothesis Diabetic retinopathy is a progressive neurodegenerative disease, but the underlying mechanism is still obscure. Here, we focused on oxidative stress in the retina, and analysed its ...influence on retinal neurodegeneration, using an antioxidant, lutein. Methods C57BL/6 mice with streptozotocin-induced diabetes were constantly fed either a lutein-supplemented diet or a control diet from the onset of diabetes, and their metabolic data were recorded. In 1-month-diabetic mice, reactive oxygen species (ROS) in the retina were measured using dihydroethidium and visual function was evaluated by electroretinograms. Levels of activated extracellular signal-regulated kinase (ERK), synaptophysin and brain-derived neurotrophic factor (BDNF) were also measured by immunoblotting in the retina of 1-month-diabetic mice. In the retinal sections of 4-month-diabetic mice, histological changes, cleaved caspase-3 and TUNEL staining were analysed. Results Lutein did not affect the metabolic status of the diabetic mice, but it prevented ROS generation in the retina and the visual impairment induced by diabetes. ERK activation, the subsequent synaptophysin reduction, and the BDNF depletion in the diabetic retina were all prevented by lutein. Later, in 4-month-diabetic mice, a decrease in the thickness of the inner plexiform and nuclear layers, and ganglion cell number, together with increase in cleaved caspase-3- and TUNEL-positive cells, were avoided in the retina of lutein-fed mice. Conclusions/interpretation The results indicated that local oxidative stress that has a neurodegenerative influence in the diabetic retina is prevented by constant intake of a lutein-supplemented diet. The antioxidant, lutein may be a potential therapeutic approach to protect visual function in diabetes.
A detailed phenomenology of low energy excitations is a crucial starting point for microscopic understanding of complex materials, such as the cuprate high-temperature superconductors. Because of its ...unique momentum-space discrimination, angle-resolved photoemission spectroscopy (ARPES) is ideally suited for this task in the cuprates, where emergent phases, particularly superconductivity and the pseudogap, have anisotropic gap structure in momentum space. We present a comprehensive doping- and temperature-dependence ARPES study of spectral gaps in Bi ₂Sr ₂CaCu ₂O ₈₊δ, covering much of the superconducting portion of the phase diagram. In the ground state, abrupt changes in near-nodal gap phenomenology give spectroscopic evidence for two potential quantum critical points, p = 0.19 for the pseudogap phase and p = 0.076 for another competing phase. Temperature dependence reveals that the pseudogap is not static below T c and exists p > 0.19 at higher temperatures. Our data imply a revised phase diagram that reconciles conflicting reports about the endpoint of the pseudogap in the literature, incorporates phase competition between the superconducting gap and pseudogap, and highlights distinct physics at the edge of the superconducting dome.
In order to clarify the taxonomic position of serotypes 20, 22 and 26 of Streptococcus suis , biochemical and molecular genetic studies were performed on isolates (SUT-7, SUT-286 ᵀ, SUT-319, SUT-328 ...and SUT-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. Comparative recN gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain S. suis NCTC 10234 ᵀ, i.e. 74.8–75.7 % sequence similarity. The genomic relatedness between the isolates and other strains of species of the genus Streptococcus , including S. suis , was calculated using the average nucleotide identity values of whole genome sequences, which indicated that serotypes 20, 22 and 26 should be removed taxonomically from S. suis and treated as a novel genomic species. Comparative sequence analysis revealed 99.0–100 % sequence similarities for the 16S rRNA genes between the reference strains of serotypes 20, 22 and 26, and our isolates. Isolate STU-286 ᵀ had relatively high 16S rRNA gene sequence similarity with S. suis NCTC 10234 ᵀ (98.8 %). SUT-286 ᵀ could be distinguished from S. suis and other closely related species of the genus Streptococcus using biochemical tests. Due to its phylogenetic and phenotypic similarities to S. suis we propose naming the novel species Streptococcus parasuis sp. nov., with SUT-286 ᵀ (= JCM 30273 ᵀ = DSM 29126 ᵀ) as the type strain.
An ordered phase showing remarkable electronic anisotropy in proximity to the superconducting phase is now a hot issue in the field of high-transition-temperature superconductivity. As in the case of ...copper oxides, superconductivity in iron arsenides competes or coexists with such an ordered phase. Undoped and underdoped iron arsenides have a magnetostructural ordered phase exhibiting stripe-like antiferromagnetic spin order accompanied by an orthorhombic lattice distortion; both the spin order and lattice distortion break the tetragonal symmetry of crystals of these compounds. In this ordered state, anisotropy of in-plane electrical resistivity is anomalous and difficult to attribute simply to the spin order and/or the lattice distortion. Here, we present the anisotropic optical spectra measured on detwinned BaFe 2 As 2 crystals with light polarization parallel to the Fe planes. Pronounced anisotropy is observed in the spectra, persisting up to an unexpectedly high photon energy of about 2 eV. Such anisotropy arises from an anisotropic energy gap opening below and slightly above the onset of the order. Detailed analysis of the optical spectra reveals an unprecedented electronic state in the ordered phase.
We fabricated optical directional couplers with Si-wire waveguides and demonstrated their fundamental characteristics. Their coupling-length was extremely short, several micrometers, because of ...strong optical coupling between the waveguide cores. Wavelength demultiplexing functions were also demonstrated for devices with a long coupled waveguide. Optical output from a device 800 μm long changed reciprocally with 2.5-nm wavelength spacing between the parallel and cross ports.
The authors calculated the progression rate (DeltaFS) using the total revised ALS Functional Rating Scale (ALSFRS-R) and symptom duration at diagnosis in 82 Japanese patients with ALS. Survival ...(death or tracheostomy) differed significantly with the DeltaFS and postdiagnostic period according to log-rank testing, but Cox proportional hazards modeling revealed no strong association between total ALSFRS-R and mortality, suggesting that the DeltaFS provides an additional predictive index beyond ALSFRS-R alone.
Abstract
We have investigated the pinning potential of high-quality single crystals of superconducting material CaKFe
4
As
4
having high critical current density and very high upper critical field ...using both magnetization relaxation measurements and frequency-dependent AC susceptibility. Preliminary studies of the superconducting transition and of the isothermal magnetization loops confirmed the high quality of the samples, while temperature dependence of the AC susceptibility in high magnetic fields show absolutely no dependence on the cooling conditions, hence, no magnetic history. From magnetization relaxation measurements were extracted the values of the normalized pinning potential
U
*, which reveals a clear crossover between elastic creep and plastic creep. The extremely high values of
U
*, up to 1200 K around the temperature of 20 K lead to a nearly zero value of the probability of thermally-activated flux jumps at temperatures of interest for high-field applications. The values of the creep exponents in the two creep regimes resulted from the analysis of the magnetization relaxation data are in complete agreement with theoretical models. Pinning potentials were also estimated, near the critical temperature, from AC susceptibility measurements, their values being close to those resulted (at the same temperature and DC field) from the magnetization relaxation data.
Based on the results of the IFMIF/EVEDA project in the broader approach (BA) activities, the conceptual design of the Advanced Fusion Neutron Source (A-FNS) to obtain irradiation data of DEMO blanket ...materials is under consideration in Japan. This paper describes the progress of the A-FNS conceptual design, which consists of a 40 MeV, 125 mA deuteron beam, a liquid lithium target system, and a test and post-irradiation test facilities. the main objectives of the A-FNS are the acquisition of RAFM irradiation data up to 2035 and the tritium recovery test on the blanket. In addition, it is expected to be used for various neutron researches. The technical design activities of the A-FNS are focused on improvement of the lithium target design, review of impurity criteria, remote handling, and irradiation application plan.
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