The
14
C activities and/or á
13
C values were measured for tree rings, rices, edible oils, flavors, beers, chickens and eggs. The
14
C activity in 1999 ranged from 14.4 to 14.8dpm/g.C for those of ...tree rings and rices. The
14
C activities of oils were in good agreement with the present atmospheric one. The
14
C activities of flavors could differentiate petrochemically derived compounds from natural products. On the other hand, the Δ
13
C value could distinguish between C
3
and C
4
plants for the main ingredient of oils, flavors and beers. For chickens and eggs, & Delta;
13
C values reflected their diet. In addition, large variation of Δ
13
C values was observed among components of eggs.
The recent development of the society has caused new types of problems such as gaps of depopulation, population aging, and income between urban areas and local areas. The gap of communication ...networks is also one of the problems, and the poor network circumstance in local areas might affect the future medicare or welfare services by using Information Technology. Also, when there is a natural disaster such as earthquakes or typhoon, these local areas are likely to isolate from others because of disconnection of communication cables or breakdown of network devices. Therefore, this paper proposes Autonomous Flight Wireless Nodes (AFW) for the resilient networks consisted of Delay Tolerant Networks and Never Die Networks, and it is aiming to communicate with isolated areas. In the proposed methods, IP network based drones support data transmission by the autonomous flight with seeking possible wireless stations and sending the message data by the proposed resilient network. Then, the implementations of the proposed methods are introduced, and the results of wireless network conditions by the field experiments are reported in this paper.
In this study, we implemented an Internet of Things (IoT) evacuation support
system using beaconsthat supports evacuees during disasters. This system realized quick
and accurate registration of ...safetyinformation and management of evacuation centers
without requiring special equipment by using amobile device such as a smartphone and a
beacon. Additionally, through safety information registration,this system also supports
vulnerable people such as the elderly and children by implementinga proxy safety
information registration function. Safety information registered at evacuation centersis
collected into a regional disaster prevention information/safety information management
systemmanaged by the emergency response headquarters. Furthermore, this system also
provides regionalinformation and disaster prevention information during normal use. To
evaluate the IoT evacuationsupport system, we evaluated the system usability scale and
the operability, relevance, and effectivenessof each function and many of the functions
received high evaluation scores.
In northern areas, the road conditions during the winter especially in mountain areas are one of the significant safety subjects for the residences and the tourists. In addition, there are some areas ...that the network services such as LTE or 3G are unavailable to use in mountain areas. For this reason, this paper proposed the Vehicle-to-vehicle (V2V) communication methods with the Delay Tolerant Networks (DTN) for the road surveillance system. In the proposed network system, IEEE802.11b/g/p is supposed for introducing the wireless V2V system, and the observed road condition's data is transmitted with the DTN routings. For the efficiency of the proposed network system, the GIS road maps of Takizawa in Japan are introduced for the computational simulations. Also, various DTN routings such as the Epidemic, the Spray and Wait, and the Maxprop routing are held for the future studies of the V2V based DTN routings of the road surveillance system in local areas during the winter.
In this study, we implemented an Internet of Things (IoT) evacuation support system using beacons that supports evacuees during disasters. This system realized quick and accurate registration of ...safety information and management of evacuation centers without requiring special equipment by using a mobile device such as a smartphone and a beacon. Additionally, through safety information registration, this system also supports vulnerable people such as the elderly and children by implementing a proxy safety information registration function. Safety information registered at evacuation centers is collected into a regional disaster prevention information/safety information management system managed by the emergency response headquarters. Furthermore, this system also provides regional information and disaster prevention information during normal use. To evaluate the IoT evacuation support system, we evaluated the system usability scale and the operability, relevance, and effectiveness of each function and many of the functions received high evaluation scores.
To elucidate the role of high mobility group 2 protein (HMG2) in cis‐diamminedichloroplatinum (II) (cisplatin, CDDP) sensitivity, we constructed a human HMG2‐transfected human non‐small cell lung ...cancer cell line, PC‐14/HMG2. The HMG2 mRNA expression level was approximately twice those of parental PC‐14 and mock‐transfected PC‐14/CMV. Gel mobility shift assay revealed a CDDP‐treated DNA‐protein complex in the nuclear extract of PC‐14/HMG2, which was not found in the extracts of PC‐14 and PC‐14/CMV. This complex formation was subject to competition by CDDP‐treated non‐specific salmon sperm DNA, indicating that ectopic HMG2 recognizes CDDP‐damaged DNA. PC‐14/HMG2 showed more than 3‐fold higher sensitivity to CDDP than PC‐14 and PC‐14/CMV. The intracellular platinum content of PC‐14/HMG2 after exposure to 300 μM CDDP was 1.1 and 1.5 times that of PC‐14 and PC‐14/CMV, respectively. Cellular glutathione levels were not different in these cell lines. Repair of DNA interstrand cross‐links determined by alkaline elution assay was decreased in PC‐14/HMG2. These results suggest that HMG2 may enhance the CDDP sensitivity of cells by inhibiting repair of the DNA lesion induced by CDDP.
Duocarmycins, including KW‐2189, bind in the minor groove of double‐stranded DNA at A‐T‐rich sequences, followed by covalent bonding with N‐3 of adenine in preferred sequences. We examined the effect ...of DNA‐repair modulators, such as caffeine and aphidicolin, on the cytotoxicity of duocarmycins towards human lung cancer cells, as determined by dye formation assay. Caffeine (0.5 or 1 mM), but not aphidicolin, enhanced the growth‐inhibitory activity of KW‐2189, DU‐86, and duocarmycin SA. Caffeine inhibited repair of DNA strand breaks induced by KW‐2189, as assayed by the alkaline elution technique. This suggests that duocarmycin‐induced DNA strand breaks, which are potentially lethal to cells, are repaired through a caffeine‐sensitive pathway.
The way we listen to music has undergone major changes—from cassette tapes and records to com- pact disks (CDs) and digital music that includes subscription-based online streaming and download- able ...sound sources. However, despite the popularity of digital music, CDs continue to be used as a means of listening to music due to the following reasons: the sense of safety felt upon holding the actual product and the collection factor for people interested in building personal collections. However, since the COVID-19 pandemic occurred in 2019, amid growing anxiety and turmoil about contact with “people” and “things,” users have become sensitive to the use of auditioning machines at CD shops. Therefore, we have developed a music trial listening application that uses augmented reality (AR) technology to enable people who visit CD shops to listen to the music of artists they are interested in without any physical contact with the auditioning machines. Users can feel the com- mitment of the artist by actually holding the CD jacket in their hands via AR; they can also view various types of information about the artist by recognizing the image on the CD jacket by using our AR technology-based developed application. Our proposed application consists of the “Listening Mode,” which provides the music trial listening function, and “AR Mode,” which provides the artist information presentation function. Further, we conducted an evaluation experiment with 30 sub- jects to evaluate the operability, readability, functionality, relevance, effectiveness, applicability, and safety of our developed application compared to the CD auditioning machine. The experiment results showed highly positive user evaluations regarding most of the abovementioned tested attributes. The user evaluations also showed that there is room for improvement regarding the operability of the AR objects generated via the application. In future studies, we aim to focus on the improvement of the application’s operability and enrichment of its contents, for example, we plan to add the “background playback function” and “administrator content registration function” to the existing functions of the applications.
DX‐8951f, a water‐soluble and non‐pro‐drug analogue of camptothecin, exhibits a strong inhibitory action on DNA topoisomerase I (Topo I) and in vitro cytotoxicity against various human cancer cell ...lines. In order to elucidate the mechanisms of its cytotoxicity, we established a DX‐8951f‐resistant cell line, SBC‐3/DXCL1, from human small cell lung cancer cells (SBC‐3) by stepwise exposure to DX‐8951f. SBC‐3/DXCL1 cells were approximately 400 times more resistant to DX‐8951f than parent cells. The SBC‐3/DXCL1 cells showed a high degree of cross‐resistance to other Topo I inhibitors such as CPT‐11, SN‐38 and camptothecin, but not to non‐Topo I targeting agents such as cisplatin, adriamycin, etoposide, and vincristine. The mechanisms of resistance of SBC‐3/DXCL1 cells to DX‐8951f were examined. Intracellular accumulation of DX‐8951f by SBC‐3 and SBC‐3/DXCL1 cells did not differ significantly. Although the Topo I activity of nuclear extracts obtained from SBC‐3/DXCL1 cells was the same as that of the parent cells, the Topo I of SBC‐3/DXCL1 cells was resistant to the inhibitory effects of DX8951f and SN‐38. Immunoblotting using anti‐Topo I antibody demonstrated similar protein levels of Topo I in SBC‐3 and SBC‐3/DXCL1 cells. The active Topo I protein of SBC‐3/DXCL1 was eluted by a high concentration of NaCl (0.4 N) compared with that of SBC‐3 (0.3 N). DX‐8951f stabilized the DNA‐Topo I cleavable complex from SBC‐3 cells, as measured by Topo I‐mediated cleavage assay. In SBC‐3/DXCL1 cells, DX‐8951f also stabilized the DNA‐Topo I complex, but with a 10‐fold lower efficiency. These results suggest that a qualitative change in Topo I contributes, at least partially, to the resistance to DX‐8951f in SBC‐3/DXCL1 cells. Therefore, SBC‐3/DXCL1 cells may have a unique mechanism of resistance to Topo I‐directed antitumor drugs.
In this article, we propose and evaluate a Zoo Walk Navigation System consistings of the Animal Contents Registering and Editing Web Management System and the Animal Contents Browsing and Acquiring ...Smartphone Application. The Animal Contents Registering and Editing Web Management System for zoo staff enables to register/edit various animal contents. Thereby, this web management system provides real-time and flesh zoo information to the Animal Contents Browsing and Acquiring Smartphone Application. On the other hand, the Animal Contents Browsing and Acquiring Smartphone Application for zoo visitors enables to browse various animal contents which zoo staff registered through the Animal Contents Registering and Editing Web Management System. The Animal Contents Browsing and Acquiring Smartphone Application has the animal guide browsing function, the animal quiz function, the beacon notification browsing function, the zoo map navigating function, and the AR camera function. Zoo visitors can enjoy a zoo park using this smartphone application. This system is the new type navigation system which zoo staff can renew contents to avoid contents obsolescence. And, this system always provides new information to zoo visitors in real time by the beacon notification function.
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