Recent studies suggest that Peroxiredoxin 1 (Prdx1), in addition to its known H₂O₂-scavenging function, mediates cell signaling through redox-specific protein-protein interactions. Our data ...illustrate how Prdx1 specifically coordinates p38MAPK-induced signaling through regulating p38MAPKα phosphatases in an H₂O₂ dose-dependent manner. MAPK phosphatases (MKP-1 and/or MKP-5), which are known to dephosphorylate and deactivate the senescence-inducing MAPK p38α, belong to a group of redox-sensitive phosphatases (protein tyrosine phosphatases) characterized by a low pKa cysteine in their active sites. We found that Prdx1 bound to both MKP-1 and MKP-5, but dissociated from MKP-1 when the Prdx1 peroxidatic cysteine Cys52 was over-oxidized to sulfonic acid, which in turn resulted in MKP-1 oxidation-induced oligomerization and inactivity toward p38MAPKα. Conversely, over-oxidation of Prdx1-Cys52 was enhancing in the Prdx1:MKP-5 complex with increasing amounts of H₂O₂ concentrations and correlated with a protection from oxidation-induced oligomerization and inactivation of MKP-5 so that activation toward p38MAPK was maintained. Further examination of this Prdx1-specific mechanism in a model of reactive oxygen species-induced senescence of human breast epithelial cells revealed the specific activation of MKP-5, resulting in decreased p38MAPKα activity. Taken together, our data suggest that Prdx1 orchestrates redox signaling in an H₂O₂ dose-dependent manner through the oxidation status of its peroxidatic cysteine Cys52.
Modern technology challenges anecdotal beliefs on baseball performance. The study's purpose examines these beliefs by classifying batted ball outcomes. Three categories of independent variables ...(anthropometry, in-game situation, technique-based), from 1,922 batted ball outcomes produced by 230 players, were used to classify the likelihood of hits during 2021 college baseball games. Anthropometry included player's heights and weights. In-game situation entailed batter side, same side, ahead count, and pitch type. Technique-based variables measured by TrackMan radar included exit speed (ExSp), launch angle (LA), batted ball distance (BBD), and hang time (HT). Binary logistic regression analysis was performed with batted ball outcome as the dependent variable. Independent variables provided a good fit (χ
2
(10) = 522.358, p < 0.01) and correctly classified nearly three-fourths of outcomes. Height (β = 0.030, p < 0.05), ExSp (β = 0.023, p < 0.05), LA (β = 0.028, p < 0.01), and BBD (β = 0.067, p < 0.01) each had significant positive associations, yet HT (β = -1.661, p < 0.01) had a significant negative association, with batted ball outcomes. TrackMan provided four significant independent variables. Anthropometry's contribution to batting outcome was modest, while in-game situation's impact was non-significant; results contradict anecdotal beliefs of their importance.
The purpose of this investigation was to characterize the arterial stiffness of male ultra-marathon runners (n = 9) using pulse wave velocity (cfPWV) and radial tonometry over the course of an ...ultra-marathon and during recovery. Measures were collected at rest, immediately following 45 km/75 km of running, then following 60 and 90 min of recovery. No statistical difference was found between baseline cfPWV and normative values. The cfPWV of ultra-endurance runners decreased at 45 km (3.4 ± 1.6 m/s, p=0.006), followed by an increase (1.6 ± 1.8 m/s, p = 0.04) toward baseline levels at the 75 km mark. Radial tonometry measures also indicated small artery stiffness was transiently increased after 75 km. The amount of training time (r = 0.82, p = 0.007) and the duration of a typical training session (r = 0.73, p = 0.03) were correlated strongly with persisting decrements in large artery compliance at 60 min of recovery. The finding that arterial stiffness decreased at the 45 km distance and then reverted back toward baseline levels with prolonged running, may indicate a role of exercise duration or accumulated stress for affecting vascular compliance. At present, it is premature to suggest that athletes should alter training or racing practices to protect vascular health.
There is a growing interest in training for and competing in race distances that exceed the marathon; however, little is known regarding the vascular effects of participation in such prolonged ...events, which last multiple consecutive hours. There exists some evidence that cardiovascular function may be impaired following extreme prolonged exercise, but at present, only cardiac function has been specifically examined following exposure to this nature of exercise. The primary purpose of this study was to characterize the acute effects of participation in an ultra-marathon on resting systemic arterial compliance. Arterial compliance and various resting cardiovascular indices were collected at rest from 26 healthy ultra-marathon competitors using applanation tonometry (HDI CR-2000) before and after participation in a mountain trail running foot race ranging from 120-195 km which required between 20-40 continuous hours (31.2±6.8 h) to complete. There was no significant change in small artery compliance from baseline to post race follow-up (8.5±3.4-7.7±8.2 mL/mmHgx100, p=0.65), but large artery compliance decreased from 16.1±4.4 to 13.5±3.8 mL/mmHgx10 (p=0.003). Participation in extreme endurance exercise of prolonged duration was associated with acute reductions in large artery compliance, but the time course of this effect remains to be elucidated.
Objective: The validity and clinical utility of the Reynolds Adolescent Depression Scale, Beck Hopelessness Scale, Suicidal Ideation Questionnaire-Junior, and Suicide Probability Scale (SPS) were ...examined longitudinally among suicidal adolescents. Method: Between 1998 and 2000, 289 psychiatrically hospitalized, suicidal youth, ages 12 to 17 years, participated in this study. Self-report measures were completed at baseline. Clinician-rated suicidality and suicide attempt were collected at baseline and 6-month follow-up. Results: Baseline self-reports were internally consistent and strongly intercorrelated within male, female, white, and black subsamples. All of the measures predicted follow-up suicidality and suicide attempts. Using published cutoff scores, the Beck Hopelessness Scale and SPS were moderately to highly sensitive predictors of subsequent suicide attempts, as was the Suicidal Ideation Questionnaire-Junior for predicting suicide attempts and broad suicidality. Alternative cutoff scores that predicted outcomes with moderate and high sensitivity also were examined, with attention to resultant sacrifices in specificity. Conclusions: Baseline self-report scores predicted follow-up suicidality. SPS contributed uniquely to prediction of future suicidality and suicide attempt. SPS may supplement other sources of information when assessing suicide risk with this population. (Contains 4 tables.)
South Africa was the first country to deploy biological control (biocontrol) against invasive Prosopis populations. Developments in this regard have been ongoing, and have been reviewed, at ...approximately 10-year intervals, since 1991. This review spans the period 2011-2020, a timespan globally characterised by increased awareness of the impacts of invasive Prosopis populations, and recognition of the need for improved management. Concerted international collaboration has resulted in enhanced clarity on phylogenetic relationships within the Leguminosae and the phylogenetic placement of Prosopis. These advances have improved the framework for interpreting the host range of potential agents and for evaluating risk. At the outset of the biocontrol programme, in the 1980s, only agents that consumed mature seeds were considered. The intention was to reduce the invasiveness of Prosopis while simultaneously retaining it as a usable resource. The programme was subsequently expanded to investigate agents that prevent pod set or maturation of seed. More recently, potential agents that damage the vegetative growth of the plants have been included in response to recognition in South Africa, that there is no other route to successful management of Prosopis. There is a wealth of largely unexplored potential in this regard.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Internal waves drive ocean mixing and enhance the transport of heat, momentum and other tracers in shelf seas. We collected observations of mixing over a 30‐day period from three cross‐shore moorings ...placed on the 330, 200 and 150 m isobaths on the offshore side of a pelagic ridge on the Australian North West Shelf. The region is forced by energetic surface and internal tides, exhibits non‐linear internal waves, experiences flow‐topography interactions, and is subject to episodic intense wind events. This complex forcing drove energetic diapycnal mixing at all sites. We identified five dominant internal wave forcing categories: mode‐1 waves at low‐frequency (time scales from double the buoyancy period to 4 hr), mode‐1 waves at high‐frequency (HF) (time scales between the buoyancy period and double the buoyancy period), mode‐2 waves, internal bores, and internal hydraulic jumps. Overall, just 15% of mixing events accounted for 90% of the total observed heat flux over the record. Mixing during internal wave events accounted for as much as 50% of the total heat flux in some locations. Of the internal wave categories, HF mode‐1 waves were the most significant contributors to the total heat flux at all sites (∼20%). On the other hand, internal bores made significant contributions to mixing only at the 200 and 150 m moorings; they made no contribution to mixing at the 330 m mooring. At the shallowest mooring, the different internal wave categories all made similar contributions to the total flux, indicating an increasingly complicated relationship between the evolving internal wavefield and the mixing.
Plain Language Summary
Internal waves propagate along the density gradients found beneath the ocean's surface layer, analogous to surface waves propagating along the sharp density gradient where air and water meet. These waves play an important role in the distribution of nutrients, heat, contaminants, and other tracers in the ocean, especially in coastal regions where the waves break. The density structure of the ocean, the tidal and wind forcing, and the seabed features result in many different types of internal waves, each of which travel and break differently. In this work, we examine the mixing caused by different types of internal waves as they travel up and over a subsurface ridge on the Australian North West Shelf. We found that most of the significant mixing resulted from relatively rare events, which often occurred during internal wave forcing events. The magnitude of mixing increased in shallower waters due to internal waves breaking and causing energetic turbulent flows. However, the wave types that were the most significant for mixing changed depending on the location on the shelf and the depth. High‐frequency internal waves were generally the most significant contributor to mixing. Internal bores, a class of waves moving upslope near the seabed, did dominate the total ocean mixing near‐bottom at some locations.
Key Points
Energetic but short‐lived nonlinear internal wave events drove most of the vertical turbulent heat flux over the month‐long record
Internal wave events evolved over relatively short time scales and relatively short spatial scales
Energetic internal wave events contribute significantly to mixing in shelf seas
We designed a prospective feasibility study to assess the 5x-multiplier (5x) calculation (eg, 3 pills in last 24 hours × 5 = 15) to standardize discharge opioid prescriptions compared to usual care.
...Faculty-based surgical teams volunteered for either 5x or usual care arms. Patients undergoing inpatient (≥48 hours) surgery and discharged by surgical teams were included. The primary end point was discharge oral morphine equivalents. Secondary end points were opioid-free discharges and 30-day refill rates.
Median last 24-hour oral morphine equivalents was similar between arms (7.5 mg 5x vs 10 mg usual care, P = .830). Median discharge oral morphine equivalents were less in the 5x arm (50 mg 5x vs 75 mg usual care, P < .001). Opioid-free discharges included 33.5% 5x vs 18.0% usual care arm patients (P < .001). Thirty-day refill rates were similar (15.3% 5x vs 16.5% usual care, P = .742).
The 5x-multiplier was associated with reduced opioid prescriptions without increased refills and can be feasibly implemented across a diverse surgical practice.
•We conducted a feasibility study to compare a 5x-multiplier calculation of last-24-hour oral morphine equivalents (eg, 3 pills × 5 = 15 pills) to standardize discharge prescriptions versus usual care after inpatient cancer surgery.•The 5x-multiplier was associated with reduced opioid prescriptions without increased refills.•The 5x-multiplier can be feasibly implemented across a diverse surgical practice.
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