Visuospatial working memory (VSWM) helps track the identity and location of people during social interactions. Previous work showed better VSWM when all faces at encoding displayed a happy compared ...to an angry expression, reflecting a prosocial preference for monitoring who was where. However, social environments are not typically uniform, and certain expressions may more strongly compete for and bias face monitoring according to valence and/or arousal properties. Here, we used heterogeneous encoding displays in which two faces shared one emotion and two shared another, and asked participants to relocate a central neutral probe face after a blank delay. When considering the emotion of the probed face independently of the co-occurring emotion at encoding, an overall happy benefit was replicated. However, accuracy was modulated by the nonprobed emotion, with a relocation benefit for angry over sad, happy over fearful, and sad over happy faces. These effects did not depend on encoding fixation time, stimulus arousal, perceptual similarity, or response bias. Thus, emotional competition for faces in VSWM is complex and appears to rely on more than simple arousal- or valence-biased mechanisms. We propose a "social value (SV)" account to better explain when and why certain emotions may be prioritized in VSWM. (PsycInfo Database Record (c) 2024 APA, all rights reserved).
Previous research has shown that angry and happy faces are perceived as less emotionally intense when shown with averted versus direct gaze. Other work reports that long-term memory (LTM) for angry ...(but not happy) faces was poorer when they were encoded with averted versus direct gaze, suggesting that threat signals are diluted when eye contact is not engaged. The current study examined whether gaze modulates working memory (WM) for angry and happy faces. In stark contrast to LTM effects, WM for angry faces was not significantly modulated by gaze direction. However, WM for happy faces was significantly enhanced when gaze was averted versus direct. These findings suggest that in WM - when rapid processing and an immediate response is required - averted gaze may alter the meaning behind a smile, and make this kind of expression particularly salient for short-term processing.
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BFBNIB, DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Joint Attention Enhances Visual Working Memory Gregory, Samantha E. A; Jackson, Margaret C
Journal of experimental psychology. Learning, memory, and cognition,
02/2017, Letnik:
43, Številka:
2
Journal Article
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Joint attention-the mutual focus of 2 individuals on an item-speeds detection and discrimination of target information. However, what happens to that information beyond the initial perceptual ...episode? To fully comprehend and engage with our immediate environment also requires working memory (WM), which integrates information from second to second to create a coherent and fluid picture of our world. Yet, no research exists at present that examines how joint attention directly impacts WM. To investigate this, we created a unique paradigm that combines gaze cues with a traditional visual WM task. A central, direct gaze 'cue' face looked left or right, followed 500 ms later by 4, 6, or 8 colored squares presented on one side of the face for encoding. Crucially, the cue face either looked at the squares (valid cue) or looked away from them (invalid cue). A no shift (direct gaze) condition served as a baseline. After a blank 1,000 ms maintenance interval, participants stated whether a single test square color was present or not in the preceding display. WM accuracy was significantly greater for colors encoded in the valid versus invalid and direct conditions. Further experiments showed that an arrow cue and a low-level motion cue-both shown to reliably orient attention-did not reliably modulate WM, indicating that social cues are more powerful. This study provides the first direct evidence that sharing the focus of another individual establishes a point of reference from which information is advantageously encoded into WM.
Despite growing concerns about the impacts of climate change, Japanese public financial institutions continued to build on long-standing partnerships in Southeast Asia through the expansion of ...thermal power generation. Recent Japanese investments include coal-fired power plants in Indonesia and Vietnam and natural gas supply chains in Indonesia and Bangladesh. Japan was one of the last developed countries globally to champion coal development that, even if highly efficient, comes at a cost to the environment. Overseas energy investment is at the nexus of two significant challenges of this century: the threat of climate change and great power competition. Japan, regionally proximate to China and burdened with a shrinking economy and limited energy supplies, shapes its regional foreign policy to enhance energy security, expand overseas market share, and uphold a rules-based international order. This paper discusses these primary drivers of Japan’s policy approach and highlights examples of Japan’s overseas energy investment strategies starting to shift to maintain a competitive edge as public opinion and market forces react to the increased risk perception of climate change.
It is well established that visual working memory (WM) for face identity is enhanced when faces display threatening versus nonthreatening expressions. During social interaction, it is also important ...to bind person identity with location information in WM to remember who was where, but we lack a clear understanding of how emotional expression influences this. Here, we conducted two touchscreen experiments to investigate how angry versus happy expressions displayed at encoding influenced the precision with which participants relocated a single neutral test face to its original position. Maintenance interval was manipulated (Experiment 2; 1 s, 3 s, 6 s) to assess durability of binding. In both experiments, relocation accuracy was enhanced when faces were happy versus angry, and this happy benefit endured from 1-s to 6-s maintenance interval. Eye movement measures during encoding showed no convincing effects of oculomotor behavior that could readily explain the happy benefit. However, accuracy in general was improved, and the happy benefit was strongest for the last, most recent face fixated at encoding. Improved, durable binding of who was where in the presence of a happy expression may reflect the importance of prosocial navigation.
Joint focus of attention between two individuals can influence the way that observers attend, encode, and value items. Using a nonpredictive gaze cuing task we previously found that working memory ...(WM) was better for jointly attended (validly cued) versus invalidly cued colored squares. Here we examine whether this influence of gaze on WM is driven by observers sharing the perspective of the face cue (mental state account), or simply by increased attention to the cued location (social attention account). To manipulate perspective taking, a closed barrier obstructed the cue face's view of the memoranda, while an open barrier allowed the cue face to "see" the colors. A central cue face flanked by two identical barriers looked left or right, followed 500 ms later by colored squares for encoding which appeared equally often in the validly and invalidly cued locations. After a blank 1,000 ms maintenance interval, participants stated whether a probe color was present or not in the preceding display. When the barrier was open, WM was significantly impaired for invalidly versus validly cued items. When the barrier was closed, the effect of gaze cues on WM was abolished. In contrast, further experiments showed a significant cuing effect on the speed of simple target localization and color discrimination regardless of barrier type. These findings support the mental state account of joint attention in WM, whereby the attentional focus of another alters WM via higher level engagement with the second person perspective. A goal-specific model of perspective taking is proposed.
Preclinical studies using bone marrow derived cells to treat traumatic brain injury have demonstrated efficacy in terms of blood–brain barrier preservation, neurogenesis, and functional outcomes. ...Phase 1 clinical trials using bone marrow mononuclear cells infused intravenously in children with severe traumatic brain injury demonstrated safety and potentially a central nervous system structural preservation treatment effect. This study sought to confirm the safety, logistic feasibility, and potential treatment effect size of structural preservation/inflammatory biomarker mitigation in adults to guide Phase 2 clinical trial design. Adults with severe traumatic brain injury (Glasgow Coma Scale 5–8) and without signs of irreversible brain injury were evaluated for entry into the trial. A dose escalation format was performed in 25 patients: 5 controls, followed 5 patients in each dosing cohort (6, 9, 12 ×106 cells/kg body weight), then 5 more controls. Bone marrow harvest, cell processing to isolate the mononuclear fraction, and re‐infusion occurred within 48 hours after injury. Patients were monitored for harvest‐related hemodynamic changes, infusional toxicity, and adverse events. Outcome measures included magnetic resonance imaging‐based measurements of supratentorial and corpus callosal volumes as well as diffusion tensor imaging‐based measurements of fractional anisotropy and mean diffusivity of the corpus callosum and the corticospinal tract at the level of the brainstem at 1 month and 6 months postinjury. Functional and neurocognitive outcomes were measured and correlated with imaging data. Inflammatory cytokine arrays were measured in the plasma pretreatment, posttreatment, and at 1 and 6 month follow‐up. There were no serious adverse events. There was a mild pulmonary toxicity of the highest dose that was not clinically significant. Despite the treatment group having greater injury severity, there was structural preservation of critical regions of interest that correlated with functional outcomes. Key inflammatory cytokines were downregulated. Treatment of severe, adult traumatic brain injury using an intravenously delivered autologous bone marrow mononuclear cell infusion is safe and logistically feasible. There appears to be a treatment signal as evidenced by central nervous system structural preservation, consistent with previous pediatric trial data. Inflammatory biomarkers are downregulated after cell infusion. Stem Cells 2016
Video Highlight: https://youtu.be/UiCCPIe-IaQ Stem Cells 2017;35:1065–1079
Autologous bone marrow mononuclear cells (BMMNC) in escalating doses of 6, 9, 12 × 106 cells/kg were injected intravenously to treat patients with severe traumatic brain injury within 48 hours of injury and compared to concurrent, placebo‐ controlled controls in a Phase 1/2a clinical trial. Primary outcome measures included white matter volumetrics and corpus callosal (CC) fractional anisotropy (FA) as a marker of myelination/myelin loss over time. There was a strong treatment effect noted (Cohen's d) in corpus callosal FA and mean diffusivity (MD), providing powerful estimates for the follow‐on trial sample size determination. The corpus callosal fiber tractography from individual patients imaged in the early period postinjury (1 month) and at 6 months, and those images demonstrate the fiber tract drop out over time in the untreated patient that is preserved with treatment. These data are summarized in the table below.
The small intestine is traditionally viewed as an organ that mediates nutrient digestion and absorption. This view has recently been revised owing to the ability of the duodenum to sense nutrient ...influx and trigger negative feedback loops to inhibit glucose production and food intake to maintain metabolic homeostasis. Further, duodenal nutrient-sensing defects are acquired in diabetes and obesity, leading to increased glucose production. In contrast, jejunal nutrient sensing inhibits glucose production and mediates the early antidiabetic effect of bariatric surgery, and gut microbiota composition may alter intestinal nutrient-sensing mechanisms to regain better control of glucose homeostasis in diabetes and obesity in the long term. This perspective highlights nutrient-sensing mechanisms in the gut that regulate glucose homeostasis and the potential of targeting gut nutrient-sensing mechanisms as a therapeutic strategy to lower blood glucose concentrations in diabetes.
Normal social functioning depends on the ability to efficiently and accurately detect when someone's facial expression changes to convey positive or negative emotion. While observer mood state has ...been shown to influence emotion recognition, how variations in normal mood might influence sensitivity to the dynamic emergence of expressions has not yet been addressed. To investigate this, we modified an existing face-morphing paradigm in which a central face gradually changes from neutral to expressive (angry, sad, happy, surprised). Our sample comprised healthy young adults, and current mood state was measured using the PANAS-X. Participants pressed a key as soon as they (1) noticed a physical change in expression (perceptual sensitivity-novel task element), and (2) could clearly conceptualize which expression was emerging (conceptual sensitivity). A final unspeeded response required participants to explicitly label the expression as a measure of recognition accuracy. We measured the percentage morph (expression intensity) at which a perceptual and conceptual change was detected, where greater intensity equates to poorer sensitivity. Increased positive mood reduced perceptual and conceptual sensitivity to angry and sad expressions only (a mood incongruency effect). Of particular interest, increased negative mood decreased conceptual sensitivity for all expressions, but had limited impact on perceptual sensitivity. Thus, heightened negative mood is particularly detrimental for effectively decoding someone else's mood change. This may reflect greater introspection and consumption of attentional resources directed toward the negative self, leaving fewer resources to process emotional signals conveyed by others. This could have important consequences for human social interaction.
C-tag TNF: a reporter system to study TNF shedding Pinci, Francesca; Gaidt, Moritz M.; Jung, Christophe ...
Journal of biological chemistry/The Journal of biological chemistry,
12/2020, Letnik:
295, Številka:
52
Journal Article
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TNF is a highly pro-inflammatory cytokine that contributes not only to the regulation of immune responses but also to the development of severe inflammatory diseases. TNF is synthesized as a ...transmembrane protein, which is further matured via proteolytic cleavage by metalloproteases such as ADAM17, a process known as shedding. At present, TNF is mainly detected by measuring the precursor or the mature cytokine of bulk cell populations by techniques such as ELISA or immunoblotting. However, these methods do not provide information on the exact timing and extent of TNF cleavage at single-cell resolution and they do not allow the live visualization of shedding events. Here, we generated C-tag TNF as a genetically encoded reporter to study TNF shedding at the single-cell level. The functionality of the C-tag TNF reporter is based on the exposure of a cryptic epitope on the C terminus of the transmembrane portion of pro-TNF on cleavage. In both denatured and nondenatured samples, this epitope can be detected by a nanobody in a highly sensitive and specific manner only upon TNF shedding. As such, C-tag TNF can successfully be used for the detection of TNF cleavage in flow cytometry and live-cell imaging applications. We furthermore demonstrate its applicability in a forward genetic screen geared toward the identification of genetic regulators of TNF maturation. In summary, the C-tag TNF reporter can be employed to gain novel insights into the complex regulation of ADAM-dependent TNF shedding.
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