Angiomotin is a newly discovered molecule that regulates the migration and tubule formation of endothelial cells. It therefore has been implicated in the control of angiogenesis under physiological ...and pathological conditions. This study examined the expression of angiomotin and its analogues, angiomotin-like 1 (L1) and -like 2 (L2) in breast tumour tissues, and analysed their correlation with angiogenesis and clinical outcomes.
Human breast tissues (normal n = 32 and tumours n = 120) were used. The levels of expression of angiomotin, L1 and L2 were determined using reverse transcription PCR. Microvessels were stained using antibodies against PECAM, von Willebrand factor (factor 8, or vWF) and VE-cadherin. The transcript levels of angiomotin and its analogues were assessed against the clinical and pathological background, including long term survival (120 months).
Breast cancer tissues expressed significantly higher levels of angiomotin transcript, compared with normal mammary tissues (33.1 +/- 11 in normal versus 86.5 +/- 13.7 in tumour tissues, p = 0.003). Both L1 and L2 were seen at marginally higher levels in tumour than normal tissues but the difference was not statistically significant. Levels of angiomotin were at significantly higher levels in grade 2 and grade 3 tumours compared with grade 1 (p < 0.01 and p = 0.05 respectively). The levels of angiomotin in tumours from patients who had metastatic disease were also significantly higher than those patients who remained disease free (p = 0.03). Multivariate analysis indicated that angiomotin transcript was an independent prognostic factor (p = 0.031). No significant correlations were seen between angiomotin-L1 and L2 with the clinical outcome. Furthermore, high levels of angiomotin transcript were associated with shorter overall survival (p < 0.05). There was a high degree of correlation between levels of vW factor and that of angiomotin (p < 0.05), but not angiomotin-L1 and angiomotin-L2.
Angiomotin, a putative endothelial motility factor, is highly expressed in human breast tumour tissues and linked to angiogenesis. It links to the aggressive nature of breast tumours and the long term survival of the patients. These data point angiomotin as being a potential therapeutic target.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
This study was conducted to explore the regulatory role of the target of rapamycin complex 1 (TORC1) signaling pathway in crop milk synthesis in breeding pigeons (Columba livia). Three groups of ...breeding pigeons in the lactation period (n = 30 pairs/group) were respectively injected with rapamycin (RAPA, a specific inhibitor of the target of rapamycin complex) at doses of 0 (vehicle, control), 0.6, or 1.2 mg/kg body weight (BW)/day via the wing vein for 7 days. The average daily feed intake (ADFI) and BW of the breeding pigeons and the BW of young squabs were respectively recorded throughout the experimental period. The breeding pigeons were sacrificed to collect their crop tissues, crop milk, and serum on the eighth day of the experiment. The results showed that neither 0.6 nor 1.2 mg/kg BW RAPA injection affected BW loss or ADFI in breeding pigeons (P > 0.05), while crop thickness and crop relative weight were significantly decreased (P < 0.05) in the 1.2 mg/kg BW rapamycin-injected group. Simultaneously, RAPA (especially at 1.2 mg/kg BW) decreased the crude protein, αs1-casein, αs2-casein, β-casein, and amino acid contents (Asp, Thr, Ser, Glu, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Lys, His, Arg, and Pro) of crop milk (P < 0.05) and the concentrations of albumin, total protein, and uric acid in the serum of breeding pigeons (P < 0.05). Additionally, the expression of TORC1 pathway-related proteins (TORC1, S6K1, S6, 4EBP1, and eIF4E) was downregulated in the crop tissues of breeding pigeons by 0.6 or 1.2 mg/kg BW/day RAPA injection (P < 0.05). Accordingly, the average daily gain (ADG) of young squabs declined, and the mortality rate increased significantly (P < 0.05). Together, the results showed that RAPA reduced protein and amino acid levels in the crop milk of breeding pigeons and retarded young squab growth, suggesting a crucial role of TORC1 in crop milk synthesis in breeding pigeons.
MT1-MMP (membrane type-1 matrix metalloproteinase), otherwise known as
MMP14 is a proteolytic enzyme known to be involved in degradating extracellular
matrix and assist progression of cancer invasion ...and progression. We investigated
the impact of targeting the expression of MT1-MMP in breast cancer and its clinical
relevance. Human breast cancer cell line MDA-MB-231 was used. Expression of MT1-MMP
in the breast cancer cell line was manipulated by way of retroviral ribozyme transgene.
The in vitro invasion, growth and cell migration were determined on cell lines
transfected with either the transgene or control plasmid. Protein and message
levels of MMP14 was also assessed using immunohistochemistry and real-time quantitative
analysis, and correlated with clinical and pathological information of the patients.
Retroviral ribozyme transgene to human MT1-MMP successfully knocked down the levels
of MT1-MMP mRNA from MDA-MB-231 cells. Reduction of MT1-MMP from the breast cancer
cells resulted in significant reduction of in vitro invasiveness and loss of response
to an invasion stimulus, HGF, compared with control and wild-type cells. The invasion
index for MT1-MMP knockdown cells were 13±3.1 (without HGF) and 16.4±2.3 (with
HGF, p=0.14), and the index for transfection control cells 25.3±4.3 (without HGF)
and 40.4±4.1 (with HGF, p=0.0049). Transfection with the transgenes did not change
the rate of cell growth. In clinical breast cancer, MT1-MMP staining was both
membranous and cytoplasmic. Tumour cells displayed stronger staining compared
with normal mammary epithelial cells. Tumour tissues had a marginally higher levels
of the MMP14 transcript (8.6±1.9), compared with normal tissues (4.7±1.4), p=0.13.
No significant difference was observed between node positive and node negative
tumours (9.0±2.2 vs 8.7±3.1, p=0.24). Marginally higher levels of the MMP14 transcript
were seen in tumours which developed metastasis and local recurrence. However,
tumours from patients who died of breast cancer related causes had significantly
higher levels of the transcript, compared with tumours from patients who remained
disease-free 10 years after initial surgery (12.2±2.5 vs 6.3±1.2, p=0.0091). MT1-MMP
is a proteolytic enzyme that is pivotal in controlling the invasiveness of breast
cancer cells. It is highly expressed in aggressive breast tumours and is associated
with clinical outcome. The enzyme is a potential therapeutic target in breast
cancer.
The surgical treatment of pulmonary aspergilloma is challenging and controversial. This study was designed to evaluate the clinical profile, indications and surgical outcomes of pulmonary ...aspergilloma operated on in our institute. A total of 256 patients with pulmonary aspergilloma underwent surgical treatment from 1975 to 2010. The patients were divided into two groups: Group A (simple aspergilloma, n = 96) and Group B (complex aspergilloma, n = 160). The principal underlying lung disease was tuberculosis (71.1%). The surgical procedures consisted of 212 lobectomies in both groups; eight cavernoplasties, 10 bilobectomies, 16 pneumonectomies and six thoracoplasties in Group B; four segmentectomies and six wedge resections in Group A. Postoperative complications occurred in 40 patients (15.6%). The major complications were residual pleural space (3.9%), prolonged air leak (3.1%), bronchopleural fistula (1.6%), excessive bleeding (1.6%), respiratory insufficiency (1.9%) and empyema (1.2%). No intraoperative deaths occurred. The overall mortality within 30 days post-operation was 1.2%, occurring only in Group B. There was no statistically significant difference in the postoperative morbidity between Groups A and B (P = 0.27). With the good selection of patients, meticulous surgical techniques and good postoperative management, aggressive surgical treatment with anti-fungal therapy for pulmonary aspergilloma is safe and effective, and can achieve favourable outcomes.
To compare the short-term and long-term outcomes of robotic rectectomy and laparoscopic rectectomy for rectal cancer based on propensity score matching.
The clinical data of 106 patients who ...underwent robotic or laparoscopic radical resection of rectal cancer at Department of General Surgery, the First Affiliated Hospital of Nanchang University from January 2015 to December 2015 were retrospectively collected. Propensity score matching method was used to perform 1∶1 matching between robot and laparoscopic rectal cancer radical surgery. Thirty-two patients in robot group and 32 patients in laparoscopic group were successfully matched. There were 15 males and 17 females in the robotic group, aging (56.2±7.5) years, 19 males and 13 females in the laparoscopic group, aged (55.5±7.6) years. The clinical outcome of the two groups were compared using
-test or Mann-Whitney
test for continuous variables, repeated measures analysis of variance, χ(2) test, Fisher exact test or Wilcoxon rank sum test for dichotomous vari
The estrogenic pollutants 4-
tert-octylphenol (OP), 4-nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted ...and concentrated from 300 ml acidified water samples by liquid–liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography–mass spectrometry (GC–MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 μg
l
−1) and NP (0.55 μg
l
−1). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0–20.2, 106–296 and 19.1–106 ng
l
−1, respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%.
Four color light curves of the EW type eclipsing binary V441 Lac were presented and analyzed by the W–D code. It is found that V441 Lac is an extremely low mass ratio (
q
= 0.093±0.001) semi-detached ...binary with the less massive secondary component filling the inner Roche lobe. Two dark spots on the primary component were introduced to explain the asymmetric light curves. By analyzing all times of light minimum, we determined that the orbital period of V441 Lac is continuously increasing at a rate of d
P
/d
t
= 5.874(±0.007) × 10
−7
d yr
−1
. The semi-detached Algol type configuration of V441 Lac is possibly formed by a contact configuration destroyed shallow contact binary due to mass transfer from the less massive component to the more massive one predicted by the thermal relaxation oscillation theory.
Drilling Fluid from Natural Vegetable Gum Li, F. X.; Jiang, G. C.; Wang, Z. K. ...
Petroleum science and technology,
03/2014, Letnik:
32, Številka:
6
Journal Article
Recenzirano
Natural vegetable gum drilling fluids with high-temperature resistance are developed for environmental protection. The vegetable gum is chosen as raw material. All agents such as major treatment ...agent TLJ-1 and auxiliary treatment agents LV-CMC, PEG, and QS-2 are friendly to environment. By changing the physical and chemical environment of the fluid, temperature resistance capability of the fluid is studied. Laboratory tests show the performance of the fluid.
Purpose: The type II transmembrane serine proteases are cell surface proteolytic enzymes that mediate a diverse range of cellular
functions, including tumor invasion and metastasis. Matriptase ...(matriptase-1) and matriptase-2 belong to the type II transmembrane
serine protease family. Matriptase-1 is known to play a role in breast cancer progression, and elevated levels of matriptase-1
correlate with poor patient outcome. The role of matriptase-2 and its cellular function in cancer is unknown. This study aimed
to provide new insights into the significance of matriptase-2 in cancer.
Experimental Design: Matriptase-2 expression levels were assessed in a cohort of human breast cancer specimens (normal, n = 34; cancer, n = 95), in association with patient clinical variables, using both quantitative and qualitative analysis of the matriptase-2
transcript along with immunohistochemical techniques. Matriptase-2 was also experimentally overexpressed in the MDA-MB-231
human breast cancer cell line. The effects of matriptase-2 overexpression were examined through a series of in vitro and in vivo studies.
Results: Here, we show that reduced matriptase-2 levels in breast cancer tissues correlate with an overall poor prognosis for the
breast cancer patient. This study also reveals that matriptase-2 overexpression in breast cancer cells significantly suppressed
tumorigenesis in CD1 athymic mice ( P = 0.000003). Furthermore, we report that matriptase-2 overexpression dramatically reduced the invasive ( P = 0.0001) and migratory properties ( P = 0.01) of the breast cancer cells.
Conclusions: Matriptase-2 suppresses breast tumor development in vivo , displays prognostic value for breast cancer patients, inhibits both breast cancer cell invasion and motility in vitro , and may play a contrasting role to matriptase-1 in breast cancer.
Novel physically crosslinked polyurethane (PUII) based on isophorone diisocyanates (IPDI) was prepared by a conventional two step method. The chemical structures of the PUII were characterized by ...fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H-NMR), gel permeation chromatography (GPC) and scanning electron microscopy (SEM). The PUII hydrogels were subjected to solvent-induced self-assembly in THF + water to construct a variety of morphologies. The self-assembly morphology of the PUII was observed by scanning electron microscopy (SEM). Different amounts (0.2%, 0.4%, 0.6%, 0.8%, 1.0%) of 1,3,5-Tris(2-hydroxyethyl)hexahydro-1,3,5-triazine (TNO) was added as antibacterial agent to the polyurethane prepolymers. The inhibiting capacity of the antibacterial films to the Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Gray mold has been studied. The inhibiting capacity of films for each strain effect became obvious with the increase of content of antibacterial agent and the sensitive degree to all kind of bacterial species was different.
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