Porous Co3O4/reduced graphene oxide (RGO) composites with excellent supercapacitive performance were successfully synthesized on nickel foam (NF) by electrophoretic deposition followed by thermal ...reduction, and a hydrothermal method. The effects of the introduction of RGO and molar ratio of hexamethylenetetramine (HMT) and Co(NO3)2 on morphology and electrochemical performance of the deposits were investigated. The morphological evolution of Co3O4 prepared on RGO with the increase in molar ratio was followed: dispersed flower-like clusters composed of fine nanosheets (1:2) → coarse flower-like clusters (1:1) → a honeycomb-like structure consisting of fine nanosheets (2:1) → two layers of structures (initial honeycomb-like structure covered with coarse flower-like structure in 3:1 and 4:1). A highest specific surface area was obtained in the sample prepared in the HMT/Co(NO3)2 M ratio of 2:1. The introduction of RGO contributed to the increase in specific surface area of the deposits. For the three-electrode testing system, the specific capacitance of the Co3O4/RGO deposit at the molar ratio of 2:1 reached the highest values of 1138.11 F g−1 at a current density of 1 A g−1, 800.71 F g−1 at a scanning rate of 5 mV s−1, which were higher than those of Co3O4. This Co3O4/RGO deposit also demonstrated the outstanding cycling stability (the specific capacitance of the 80.67% of initial value was retained after 5000 cycles). For the two-electrode testing system, the as-fabricated asymmetric supercapacitor device achieved a high specific capacitance of 108.87 F g−1 at a current density of 1 A g−1 with a stable operational voltage of 1.45 V and a high energy density of 647.05 Wh⋅kg−1.
•RGO/Co3O4 composites with excellent electrochemical performance were prepared by a new two-step method.•The morphological evolution of Co3O4/RGO with the change in mole ratio of HMT/Co(NO3)2 was investigated.•The relationship between morphology and electrochemical performance was established.
Plasma DNA is predominantly hematopoietic in origin. The size difference between maternal- and fetal-derived DNA in maternal plasma prompted us to investigate whether there was any discrepancy in ...molecular size between hematopoietically and nonhematopoietically derived DNA in plasma.
Plasma DNA samples from 6 hematopoietic stem cell transplant recipients and 1 liver transplant recipient were analyzed by massively parallel paired-end sequencing. The size of each fragment was deduced from the alignment positions of the paired reads. In sex-mismatched transplant recipients, the reads from chromosome Y were used as markers for the male donor/recipient. For other transplant recipients, the reads of the donor- and recipient-specific alleles were identified from the single-nucleotide polymorphism genotypes.
In male patients receiving female hematopoietic stem cells, more chromosome Y-derived DNA molecules (nonhematopoietically derived) were ≤150 bp than the autosome-derived ones (mainly hematopoietically derived) (median difference, 9.9%). In other hematopoietic stem cell transplant recipients, more recipient-specific DNA molecules (nonhematopoietically derived) were ≤150 bp than the donor-specific ones (hematopoietically derived) (median difference, 14.8%). In the liver transplant recipient, more donor-derived DNA molecules (liver derived) were ≤150 bp than the recipient-derived ones (mainly hematopoietically derived) (difference, 13.4%). The nonhematopoietically derived DNA exhibited a reduction in a 166-bp peak compared with the hematopoietically derived DNA. A 10-bp periodicity in size distribution below approximately 143 bp was observed in both DNA populations.
Massively parallel sequencing is a powerful tool for studying posttransplantation chimerism. Plasma DNA molecules exhibit a distinct fragmentation pattern, with the nonhematopoietically derived molecules being shorter than the hematopoietically derived ones.
•1Re-(Ni,Pt)Al coating was prepared by electroplating and arc ion plating.•Adding Re reduced the oxidation rate and extent of β to γ′ phase degeneration.•Doping Re in the coating had advantageous ...effects on the morphologies of TGO.•The spallation and rumpling behavior of TGO could be relieved by Re doping.•Formation of NiAl2O4 spinel was avoided due to the existence of pure Ni layer.
Two β-(Ni,Pt)Al coatings with or without Re doping and a PtAl diffusion coating were prepared on a single crystal Ni-based superalloy. Isothermal oxidation behavior of all the coatings was investigated at 1100 ℃ for 300 h. The Re doped β-(Ni,Pt)Al coating possessed the best isothermal oxidation resistance because Re reduced the oxidation rate and extent of β to γ′ phase degeneration. The oxidation mechanism of the PtAl diffusion coating and advantageous roles of Re on the effect of the morphologies, anti-spallation and rumpling behavior of thermally grown oxide (TGO) were discussed as well.
ZNF750 controls epithelial homeostasis by regulating epidermal-differentiation genes, a role underscored by its pathogenic mutations in esophageal squamous cell cancers (SCCs). However, the precise ...role of ZNF750 in SCC cell biology remains unclear. In this study, we report that ZNF750 is exclusively deleted, mutated and underexpressed in human SCCs, and low ZNF750 expression is associated with poor survival. Restoration of wildtype, but not mutant ZNF750 protein uniquely inhibited the malignant phenotypes of SCC cells both in vitro and in vivo. Notably, ZNF750 promoted the expression of a long non-coding RNA (TINCR), which mediated both cancer-inhibition and differentiation-induction effects of ZNF750. In addition, ZNF750 potently suppressed cell migration by directly inhibiting the transactivation of LAMC2. Together, our findings characterize ZNF750 as a crucial SCC-specific suppressor and uncover its novel anticancer-associated functions.
Triglycerides (TG) not only provide energy for infants but have important physiological functions. Understanding the composition and structure of TG in human milk is conducive to the development of ...infant formulas. In this study, TG species in human milk from 3 provincial capitals (Zhengzhou, Wuhan, and Harbin) in different regions of China were determined through C18 HPLC electrospray ionization tandem mass spectrometry (MS). The results showed that in human milk from these 3 regions, oleoyl-palmitoyl-linoleoylglycerol (OPL; 16.55, 19.20, and 18.67%, respectively) was more abundant than oleoyl-palmitoyl-oleoylglycerol (OPO; 10.08, 10.22, and 12.03%, respectively). Subsequently, regioisomeric and enantiomeric analysis of main TG in the human milk were performed on silver ion and chiral HPLC atmospheric pressure chemical ionization mass spectrometry (APCI)-MS, respectively. The results showed that rac-OPL (above 85%), rac-OPO (above 85%), rac-palmitoyl-oleoyl-oleoylglycerol (PPO; above 90%), and rac-OLaO (above 70%) were the main regioisomers of OPL, OPO, PPO, and lauroyl-oleoyl-oleoylglycerol (LaOO), respectively. The relative ratios of enantiomer pairs of rac-OPL (rac-OPL1 and rac-OPL2) were about 37 and 63%, respectively.
Aims
This study evaluates flonicamid biotransformation ability of Aminobacter sp. CGMCC 1.17253 and the enzyme catalytic mechanism involved.
Methods and Results
Flonicamid transformed by resting ...cells of Aminobacter sp. CGMCC 1.17253 was carried out. Aminobacter sp. CGMCC 1.17253 converts flonicamid into N‐(4‐trifluoromethylnicotinoyl) glycinamide (TFNG‐AM). Aminobacter sp. CGMCC 1.17253 transforms 31·1% of the flonicamid in a 200 mg l−1 conversion solution in 96 h. Aminobacter sp. CGMCC 1.17253 was inoculated in soil, and 72·1% of flonicamid with a concentration of 0·21 μmol g−1 was transformed in 9 days. The recombinant Escherichia coli expressing Aminobacter sp. CGMCC 1.17253 nitrile hydratase (NHase) and purified NHase were tested for the flonicamid transformation ability, both of them acquired the ability to transform flonicamid into TFNG‐AM.
Conclusions
Aminobacter sp. CGMCC 1.17253 transforms flonicamid into TFNG‐AM via hydration pathway mediated by cobalt‐containing NHase.
Significance and Impact of the Study
This is the first report that bacteria of genus Aminobacter has flonicamid‐transforming ability. This study enhances our understanding of flonicamid‐degrading mechanism. Aminobacter sp. CGMCC 1.17253 has the potential for bioremediation of flonicamid pollution.
The NiCrAlYSi coatings with or without Re-based diffusion barriers (DB) were prepared on K438 and K465 superalloys by electroplating Re-Ni layer, arc ion plating (AIP) NiCrAlYSi coating and following ...vacuum annealing. Isothermal oxidation behaviors of all coatings were investigated at 1100 ℃ in static air. The results showed that better oxidation resistance was achieved for NiCrAlYSi coatings with Re-based DB and for NiCrAlYSi coatings on K438 than that on K465. Re diffused into NiCrAlYSi coatings during isothermal oxidation and increased spallation resistance of oxide scale. The role of Re-based DB and the effects of substrate compositions were discussed.
•The dual roles of Re-based DB were found for NiCrAlYSi coating with it.•NiCrAlYSi coatings with DB transformed to Re modified coatings during oxidation.•Re diffusing in NiCrAlYSi coatings increased spallation resistance of oxide scale.•Cr and W in substrate had an important impact on oxidation resistance of coating.•The outward diffusion of W from substrate to Re-based DB made it more stable.
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•Two novel triazine derivatives (BTT-1 and BTT-2) were synthesized as corrosion inhibitors by a facile one-step method.•BTT-1 and BTT-2 exhibit high inhibitive performance with the ...inhibition efficiency of 97.9 % and 98.4 %.•The adsorption mechanism of BTT-1 and BTT-2 was explored by theoretical calculations in the aqueous environment.•The effect of substituents on the inhibitive performance of BTT-1 and BTT-2 was unveiled.
In this work, two triazine derivatives (BTT-1 and BTT-2) were synthesized by the simple one-step condensation of three components and used as high-efficient corrosion inhibitors to deal with the corrosion issue of carbon steel (CS) in petroleum industry. Electrochemical tests indicate that both BTT-1 and BTT-2 present superior inhibition performance with the inhibition efficiency of 97.9 % and 98.4 % at a low concentration of 0.18 mM, respectively. Quantum chemical calculations reveal that compared to BTT-1 molecule with a butyl chain, the introduction of benzyl group endows BTT-2 molecule with more adsorption sites, which favors the adsorption of BTT-2 molecule on CS surface. Furthermore, the GFN-xTB calculations demonstrate that BTT-1 and BTT-2 could adsorb on CS surface through the formation of Fe−N and Fe−S bonds. Compared to BTT-1, BTT-2 exhibits stronger adsorption on CS surface by forming more and shorter bonds with a more negative adsorption energy, which accounts for the better inhibitive performance of BTT-2.
Properties such as thermal conductivity, permeability, adsorption concentration of adsorbents are paramount for adsorption refrigeration. A novel consolidated composite CaCl2 with the matrix of ...expanded natural graphite treated with sulfuric acid (ENG–TSA) was investigated and the samples were developed according to different mass ratio of salt and different density. Results indicate that samples have very perspective heat and mass transfer performance. The highest thermal conductivity was 88.1 W/(m K), which is 22 times higher than that with the matrix of expanded natural graphite (ENG) and 400 times higher than ordinary granular CaCl2. Permeability of adsorbent was between 9.31 × 10−10 to 3.05 × 10−14 m2 while the density ranged between 300 and 500 kg/m3. Adsorption performance of composite CaCl2 was tested, and the results showed that for the samples with different density and salt mass ratio adsorption quantity ranged between 0.364 g/g to 0.4492 g/g while the cooling temperature and evaporating temperature changed from 25 to 35 °C and −10 to 15 °C, respectively. Furthermore, under the conditions of same heat source, cooling water and evaporating temperature, the heating time for the tube adsorber of composite CaCl2 with ENG–TSA as the matrix was almost 2.5 times less than that with ENG as the matrix.
•Consolidated composite CaCl2/ENG-TSA was studied.•The highest thermal conductivity of plate sample was 88.1 W/(m K).•The permeability CaCl2/ENG–TSA ranged from 9.31 × 10−10 to 3.05 × 10−14 m2.•The maximum cycle adsorption quantity reached 0.4455 g/g.•SCP of CaCl2/ENG–TSA improved by 48% compared with CaCl2/ENG.
The pharmacokinetics and oral bioavailability of tylosin tartrate and tylosin phosphate were carried out in broiler chickens according to a principle of single dose, random, parallel design. The two ...formulations of tylosin were given orally and intravenously at a dose level of 10 mg/kg b.w to chicken after an overnight fasting (n = 10 chickens/group). Serial blood samples were collected at different time points up to 24 h postdrug administration. A high performance liquid chromatography method was used for the determination of tylosin concentrations in chicken plasma. The tylosin plasma concentration's time plot of each chicken was analyzed by the 3P97 software. The pharmacokinetics of tylosin was best described by a one‐compartmental open model 1st absorption after oral administration. After intravenous administration the pharmacokinetics of tylosin was best described by a two‐compartmental open model, and there were no significant differences between tylosin tartrate and tylosin phosphate. After oral administration, there were significant differences in the Cₘₐₓ (0.18 ± 0.01, 0.44 ± 0.09) and AUC (0.82 ± 0.05, 1.57 ± 0.25)between tylosin phosphate and tylosin tartrate. The calculated oral bioavailability (F) of tylosin tartrate and tylosin phosphate were 25.78% and 13.73%, respectively. Above all, we can reasonably conclude that, the absorption of tylosin tartrate is better than tylosin phosphate after oral administration.
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