Computational thinking (CT) is being located at the focus of educational innovation, as a set of problem-solving skills that must be acquired by the new generations of students to thrive in a digital ...world full of objects driven by software. However, there is still no consensus on a CT definition or how to measure it. In response, we attempt to address both issues from a psychometric approach. On the one hand, a Computational Thinking Test (CTt) is administered on a sample of 1,251 Spanish students from 5th to 10th grade, so its descriptive statistics and reliability are reported in this paper. On the second hand, the criterion validity of the CTt is studied with respect to other standardized psychological tests: the Primary Mental Abilities (PMA) battery, and the RP30 problem-solving test. Thus, it is intended to provide a new instrument for CT measurement and additionally give evidence of the nature of CT through its associations with key related psychological constructs. Results show statistically significant correlations at least moderately intense between CT and: spatial ability (r = 0.44), reasoning ability (r = 0.44), and problem-solving ability (r = 0.67). These results are consistent with recent theoretical proposals linking CT to some components of the Cattel-Horn-Carroll (CHC) model of intelligence, and corroborate the conceptualization of CT as a problem-solving ability.
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•A Computational Thinking Test (CTt) aimed at Grades 5th to 10th is provided•Computational thinking correlates moderately with spatial and reasoning abilities•Computational thinking correlates strongly with problem-solving ability•Results are consistent with proposals linking CT with the CHC model of intelligence•Gender differences in computational thinking performance are discussed
In this Letter we report the error analysis of 59 exchange-correlation functionals in evaluating the structural parameters of small- and medium-sized organic molecules. From this analysis, recently ...developed double hybrids, such as xDH-PBE0, emerge as the most reliable methods, while global hybrids confirm their robustness in reproducing molecular structures. Notably the M06-L density functional is the only semilocal method reaching an accuracy comparable to hybrids’. A comparison with errors obtained on energetic databases (including thermochemistry, reaction barriers, and interaction energies) indicate that most of the functionals have a coherent behavior, showing low (or high) deviations on both energy and structure data sets. Only a few of them are more prone toward one of these two properties.
Ceftazidime-avibactam (CAZ-AVI) is a recently approved β-lactam-β-lactamase inhibitor combination with the potential to treat serious infections caused by carbapenem-resistant organisms. Few patients ...with such infections were included in the CAZ-AVI clinical trials, and clinical experience is lacking. We present a case series of patients with infections caused by carbapenem-resistant Enterobacteriaceae (CRE) or Pseudomonas aeruginosa (CRPa) who were treated with CAZ-AVI salvage therapy on a compassionate-use basis. Physicians who had prescribed CAZ-AVI completed a case report form. We used descriptive statistics to summarize patient characteristics and treatment outcomes. We used the Wilcoxon rank sum test and Fisher's exact test to compare patients by treatment outcome. The sample included 36 patients infected with CRE and two with CRPa. The most common infections were intra-abdominal. Physicians categorized 60.5% of patients as having life-threatening infections. All but two patients received other antibiotics before CAZ-AVI, for a median of 13 days. The median duration of CAZ-AVI treatment was 16 days. Twenty-five patients (65.8%) concurrently received other antibiotics to which their pathogen was nonresistant in vitro Twenty-eight patients (73.7%, 95% confidence interval CI, 56.9 to 86.6%) experienced clinical and/or microbiological cure. Five patients (20.8%) with documented microbiological cure died, whereas 10 patients (71.4%) with no documented microbiological cure died (P = 0.01). In three-quarters of cases, CAZ-AVI (alone or combined with other antibiotics) cured infections caused by carbapenem-resistant organisms, 95% of which had failed previous therapy. Microbiological cure was associated with improved survival. CAZ-AVI shows promising clinical results for infections for which treatment options are limited.
β-Lactamases enable resistance to almost all β-lactam antibiotics. Pioneering work revealed that acyclic boronic acids can act as 'transition state analogue' inhibitors of nucleophilic serine ...enzymes, including serine-β-lactamases. Here we report biochemical and biophysical analyses revealing that cyclic boronates potently inhibit both nucleophilic serine and zinc-dependent β-lactamases by a mechanism involving mimicking of the common tetrahedral intermediate. Cyclic boronates also potently inhibit the non-essential penicillin-binding protein PBP 5 by the same mechanism of action. The results open the way for development of dual action inhibitors effective against both serine- and metallo-β-lactamases, and which could also have antimicrobial activity through inhibition of PBPs.
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•In cDCD livers, postmortem NRP reduces biliary complications, in particular ITBL.•Postmortem NRP helps improve cDCD liver graft survival.•Use of postmortem NRP facilitates successful ...transplantation of older cDCD livers.
Although there is increasing interest in its use, definitive evidence demonstrating a benefit for postmortem normothermic regional perfusion (NRP) in controlled donation after circulatory death (cDCD) liver transplantation is lacking. The aim of this study was to compare results of cDCD liver transplants performed with postmortem NRP vs. super-rapid recovery (SRR), the current standard for cDCD.
This was an observational cohort study including all cDCD liver transplants performed in Spain between June 2012 and December 2016, with follow-up ending in December 2017. Each donor hospital determined whether organ recovery was performed using NRP or SRR. The propensity scores technique based on the inverse probability of treatment weighting (IPTW) was used to balance covariates across study groups; logistic and Cox regression models were used for binary and time-to-event outcomes.
During the study period, there were 95 cDCD liver transplants performed with postmortem NRP and 117 with SRR. The median donor age was 56 years (interquartile range 45–65 years). After IPTW analysis, baseline covariates were balanced, with all absolute standardised differences <0.15. IPTW-adjusted risks were significantly improved among NRP livers for overall biliary complications (odds ratio 0.14; 95% CI 0.06–0.35, p <0.001), ischaemic type biliary lesions (odds ratio 0.11; 95% CI 0.02–0.57; p = 0.008), and graft loss (hazard ratio 0.39; 95% CI 0.20–0.78; p = 0.008).
The use of postmortem NRP in cDCD liver transplantation appears to reduce postoperative biliary complications, ischaemic type biliary lesions and graft loss, and allows for the transplantation of livers even from cDCD donors of advanced age.
This is a propensity-matched nationwide observational cohort study performed using livers recovered from donors undergoing cardiac arrest provoked by the intentional withdrawal of life support (controlled donation after circulatory death, cDCD). Approximately half of the livers were recovered after a period of postmortem in situ normothermic regional perfusion, which restored warm oxygenated blood to the abdominal organs, whereas the remainder were recovered after rapid preservation with a cold solution. The study results suggest that the use of postmortem normothermic regional perfusion helps reduce rates of post-transplant biliary complications and graft loss and allows for the successful transplantation of livers from older cDCD donors.
Favipiravir is an antiviral agent effective against several RNA viruses. The drug has been shown to protect mice against experimental infection with a lethal dose of West Nile virus (WNV), a ...mosquito-borne flavivirus responsible for outbreaks of meningitis and encephalitis for which no antiviral therapy has been licensed; however, the mechanism of action of the drug is still not well understood. Here, we describe the potent
antiviral activity of favipiravir against WNV, showing that it decreases virus-specific infectivity and drives the virus to extinction. Two passages of WNV in the presence of 1 mM favipiravir-a concentration that is more than 10-fold lower than its 50% cytotoxic concentration (CC
)-resulted in a significant increase in mutation frequency in the mutant spectrum and in a bias toward A→G and G→A transitions relative to the population passaged in the absence of the drug. These data, together with the fact that the drug is already licensed in Japan against influenza virus and in a clinical trial against Ebola virus, point to favipiravir as a promising antiviral agent to fight medically relevant flaviviral infections, such as that caused by WNV.
In our current global warming climate, the growth of record-breaking heat waves (HWs) is expected to increase in its frequency and intensity. Consequently, the considerably growing and agglomerated ...world’s urban population becomes more exposed to serious heat-related health risks. In this context, the study of Surface Urban Heat Island (SUHI) intensity during HWs is of substantial importance due to the potential vulnerability urbanized areas might have to HWs in comparison to their surrounding rural areas. This article discusses Land Surface Temperatures (LST) reached during the extreme HW over Western North America during the boreal summer of 2021 using Thermal InfraRed (TIR) imagery acquired from TIR Sensor (TIRS) (30 m spatial resolution) onboard Landsat-8 platform and Moderate Resolution Imaging Spectroradiometer (MODIS) (1 km spatial resolution) onboard Terra/Aqua platforms. We provide an early assessment of maximum LSTs reached over the affected areas, as well as impacts in terms of SUHI over the main cities and towns. MODIS series of LST from 2000 to 2021 over urbanized areas presented the highest recorded LST values in late June 2021, with maximum values around 50 °C for some cities. High spatial resolution LSTs (Landsat-8) were used to map SUHI intensity as well as to assess the impact of SUHI on thermal comfort conditions at intraurban space by means of a thermal environmental quality indicator, the Urban Field Thermal Variance Index (UFTVI). The same high resolution LSTs were used to verify the existence of clusters and employ a Local Indicator of Spatial Association (LISA) to quantify its degree of strength. We identified the spatial distribution of heat patterns within the intraurban space as well as described its behavior across the thermal landscape by fitting a polynomial regression model. We also qualitatively analyze the relationship between both UFTVI and LST clusters with different land cover types. Findings indicate that average daytime SUHI intensity for the studied cities was typically within 1 to 5 °C, with some exceptional values surpassing 7 °C and 9 °C. During night, the SUHI intensity was reduced to variations within 1–3 °C, with a maximum value of +4 °C. The extreme LSTs recorded indicate no significant influence of HW on SUHI intensity. SUHI intensity maps of the intraurban space evidence hotspots of much higher values located at densely built-up areas, while urban green spaces and dense vegetation show lower values. In the same manner, UTFVI has shown “no” SUHI for densely vegetated regions, water bodies, and low-dense built-up areas with intertwined dense vegetation, while the “strongest” SUHI was observed for non-vegetated dense built-up areas with low albedo material such as concrete and pavement. LST was evidenced as a good marker for assessing the influence of HWs on SUHI and recognizing potential thermal environmental consequences of SUHI intensity. This finding highlights that remote-sensing based LST is particularly suitable as an indicator in the analysis of SUHI intensity patterns during HWs at different spatial resolutions. LST used as an indicator for analyzing and detecting extreme temperature events and its consequences seems to be a promising means for rapid and accurate monitoring and mapping.
Efflux transporters of the RND family confer resistance to multiple antibiotics in Gram-negative bacteria. Here, we identify and chemically optimize pyridylpiperazine-based compounds that potentiate ...antibiotic activity in E. coli through inhibition of its primary RND transporter, AcrAB-TolC. Characterisation of resistant E. coli mutants and structural biology analyses indicate that the compounds bind to a unique site on the transmembrane domain of the AcrB L protomer, lined by key catalytic residues involved in proton relay. Molecular dynamics simulations suggest that the inhibitors access this binding pocket from the cytoplasm via a channel exclusively present in the AcrB L protomer. Thus, our work unveils a class of allosteric efflux-pump inhibitors that likely act by preventing the functional catalytic cycle of the RND pump.
This book identifies the 50 key scientific articles in the field of vascular and endovascular surgery. It provides commentary to each carefully selected paper and explains why these papers are so ...important, thus providing every surgeon with the foundation stones of knowledge in this fast- moving area.
There has been an exponential increase in the volume and quality of published research relating to vascular and endovascular surgery in recent decades. Amongst thousands of articles, a small fraction is truly "game changing". Such studies form the foundations of vascular surgery today and the selection of papers within this book provide the 50 landmark papers every 21st century vascular and endovascular surgeon needs to know.
A valuable reference not only to the established surgeon, but also to vascular surgery residents and trainees, as well as to more experienced surgeons as they continue to learn new techniques and approaches and to improve their knowledge of vascular disorders and treatments. The papers provide an evidence-based resource for those surgeons preparing for professional exams and may inspire clinicians to produce new research.
Heart transplant recipients (HTr) have a higher probability of suffer from severe coronavirus disease-2019 (COVID-19) in comparison to general population, but their risk has changed over the course ...of the pandemic in relation to various factors. We conducted a prospective study including all HTr at risk of COVID-19 in a tertiary center between February 2020 and October 2022. The aim was to analyze how the prognosis (incidence of pneumonia and mortality) of COVID-19 in HTr has evolved over time, contextualizing variants, vaccination, and other treatments.
Of 308 HTr included, 124 got the infection (39.2%). COVID and non-COVID HTr had similar baseline characteristics. COVID-19 patients with pneumonia had a poorer prognosis than those with less severe presentations, with a higher rate of hospitalization (93.3 vs. 14.1%, p < .001) and death (41.0 vs. 1.2%, p < .001). Multivariate analysis identified age ≥60 years (odds ratio OR 3.65, 95% confidence interval CI 1.16-11.49, p = .027), and chronic kidney disease ≥3a (OR 4.95, 95% CI 1.39-17.54, p = .014) as predictors of pneumonia. Two-dose vaccination (OR 0.20, CI 95% 0.05-0.72, p = .02) and early remdesivir administration (OR 0.17, CI 0.03-0.90, p = .037) were protective factors. Over the course of the pandemic considering three periods in the follow-up (prevaccination February-December 2020, postvaccination January-December 2021, and post early remdesivir indication January-October 2022), we observed a reduction in pneumonia incidence from 62% to 19% (p < .001); and mortality (from 23% to 4%, p < .001).
The prognosis of COVID-19 in HTr has improved over time, likely due to vaccination and early administration of remdesivir.
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