Rad će se usredotočiti na analizu potencijala glazbenog i vizualnog doživljaja u građanskim salonima tijekom fin de siècle. Idući ukorak s onovremenom transformacijom glazbenog iskustva, potaknutom ...uvjerenjima da izvedba instrumentalne glazbe u privatnom prostoru može stimulirati individualnu kontemplaciju, saloni krajem stoljeća postaju uređeni u skladu s načelima estetskog doživljaja koji objedinjuje auditivnu i vizualnu percepciju. Studija slučaja u ovom radu je salon obitelji Brajković iz Perasta u Boki kotorskoj, jedan od rijetkih glazbenih salona s kraja 19. stoljeća koji je sačuvao izvorno uređenje. U radu se tumači vizualna manifestacija glazbe u ovom salonu, njezina ikonografska obilježja i reprezentativna vrijednost. Interpretacija obuhvaća znanja i pristupe iz povijesti umjetnosti, a osim toga autorica će se osloniti i na spoznaje iz područja povijesti glazbe, povijesti filozofije i kulturne antropologije. Cilj rada jest analiza značenja, uloge i vrijednosti glazbenog salona kao osobitog estetskog i konceptualnog prostora koji se krajem 19. stoljeća pojavljuje na teritoriju Boke kotorske.
The highly infectious disease COVID-19 caused by the
Betacoronavirus
SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research ...projects. The international
COVID19-NMR
consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in
COVID19-NMR
and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on
covid19-nmr.com
, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
Non-enzymatic glycation and covalent modification of proteins leads to Advanced Glycation End products (AGEs). AGEs are biomarkers of aging and neurodegenerative disease, and can be induced by ...impaired neuronal signaling. The objective of this study was to investigate if manipulation of dopamine (DA) in vitro using the model protein, bovine serum albumin (BSA), and in vivo using the model organism
influences fluorescent AGEs (fAGEs) formation as an indicator of dopamine-induced oxidation events. DA inhibited fAGEs-BSA synthesis in vitro, suggesting an anti-oxidative effect, which was not observed when flies were fed DA. Feeding flies cocaine and methamphetamine led to increased fAGEs formation. Mutants lacking the dopaminergic transporter or the D1-type showed further elevation of fAGEs accumulation, indicating that the long-term perturbation in DA function leads to higher production of fAGEs. To confirm that DA has oxidative properties in vivo, we fed flies antioxidant quercetin (QUE) together with methamphetamine. QUE significantly decreased methamphetamine-induced fAGEs formation suggesting that the perturbation of DA function in vivo leads to increased oxidation. These findings present arguments for the use of fAGEs as a biomarker of DA-associated neurodegenerative changes and for assessment of antioxidant interventions such as QUE treatment.
The paper deals with aesthetic features and conceptual values of wall-painting in the salon of Brajković-Martinović palace. This is the only originally preserved salon from Perast, created in the ...second half of the 19th century. As such, the salon is observed as the precious component of the cultural heritage of Perast and Boka Kotorska, owing to its monumental values and documentative potentials. The goal of the research is to examine the iconographic characteristics of wall painting from this salon -form, style and its symbolic significations. Additionaly, examination leads to the interpretation of its representative value, which demonstrates the evocative potentials of private-space-decoration and culture of living. Accordingly,author considers the wall-painting regarding the historical cultural circumstances of the local community and the contemporaneous European cultural milieu. Wall decoration from Brajković’s salon is considered in the context of traditional and modern compound, with local and external influences.
As complexities of addictive behaviors cannot be fully captured in laboratory studies, scientists use simple addiction‐associated phenotypes and measure them in laboratory animals. Locomotor ...sensitization, characterized by an increased behavioral response to the same dose of the drug, has been extensively used to elucidate the genetic basis and molecular mechanisms of neuronal plasticity. However, to what extent it contributes to the development of addiction is not completely clear. We tested if the development of locomotor sensitization to methamphetamine affects voluntary self‐administration, and vice versa, in order to investigate how two drug‐associated phenotypes influence one another. In our study, we used the genetically tractable model organism, Drosophila melanogaster, and quantified locomotor sensitization and voluntary self‐administration to methamphetamine using behavioral tests that were developed and adapted in our laboratory. We show that flies express robust locomotor sensitization to the second dose of volatilized methamphetamine, which significantly lowers preferential self‐administration of methamphetamine. Naive flies preferentially self‐administer food with methamphetamine over plain food. Exposing flies to volatilized methamphetamine after voluntary self‐administration abolishes locomotor sensitization. We tested period null (per01) mutant flies and showed that they do not develop locomotor sensitization, nor do they show preferential self‐administration of methamphetamine. Our results suggest that there may be partially overlapping neural circuitry that regulates the expression of locomotor sensitization and preferential self‐administration to methamphetamine and that this circuitry requires a functional per gene.
Face value for locomotor sensitization in the studies of addiction has been debatable. Here, we show that in Drosophila melanogaster, locomotor sensitization to volatilized methamphetamine significantly decreases preferential self‐administration of methamphetamine. A common candidate gene required for both behaviors is the gene period.
Williams syndrome is a rare genetic neurodevelopmental disorder, and the present study aimed to gain insights into the life experiences of affected children and the support available to them. This ...qualitative study is the first to explore the experiences of mothers of children with Williams syndrome in Croatia, which is home to 11 such children based on unofficial data. Thematic analysis of semi-structured interviews showed that mothers experienced in increase in emotional connection and intimacy among family members after the birth of the affected child, and that they received support from their husbands, other family members and other parents of affected children. At the same time, the mothers reported difficulties such as adjusting and harmonizing life roles, social isolation, lack of understanding from other people and daily care for the child. These findings may encourage and guide future research on improving the quality of life of children and adults suffering from rare diseases.
Williamsov sindrom rijedak je neurorazvojni poremećaj koji ulazi u skupinu rijetkih bolesti. Cilj ovog istraživanja bio je dobiti uvid u iskustvo života i dostupne podrške za djecu koja imaju ...dijagnozu ove rijetke bolesti iz perspektive roditelja, od koje prema neslužbenim evidencijama boluje 11 osoba u RH. U radu su prikazani rezultati prvog istraživanja u Hrvatskoj u kojem su sudjelovale majke djece s Williamsovim sindromom. Riječ je o kvalitativnom istraživanju. Podaci su prikupljeni polustrukturiranim intervjuom, te su analizirani i obraðeni metodom tematske analize. Rezultati istraživanja pokazuju da sudionice imaju iskustvo veće emocionalne povezanosti i bliskosti meðu članovima obitelji od roðenja djeteta s Williamsovim sindromom, te da imaju podršku od supružnika i drugih članova obitelji i roditelja djece s istom dijagnozom. S druge strane, ova rijetka dijagnoza suočava majke s teškoćama vezanim uz usklaðivanje životnih uloga, socijalnom izolacijom i nerazumijevanjem okoline te svakodnevnom skrbi za dijete. Zaključno, rad stavlja u fokus iskustvo života majki čija djeca imaju dijagnozu Williamsovog sindroma. Rezultati daju poticaj roditeljima, oboljelima, praktičarima i znanstvenicima za daljnja istraživanja i praktične mjere koje će unaprijediti mrežu podrške za djecu i odrasle osobe oboljele od rijetkih bolesti.
In this work, I have explored basic questions related to protein structure, function, and stability, which are critical for understanding the fundamental mechanisms of biology. The dissertation is ...divided into four chapters, which are different topics I have worked on. In Chapters 1 and 2, I will discuss my work on two different antifreeze proteins, in Chapter 3 my work on the main protease from SARS-CoV-2 is presented, while Chapter 4 details the characterization of protein stability of four B. subtilis proteins. While the projects are distinct from one another, they all contribute to deciphering the molecular basis of life processes and advancing our understanding of protein biochemistry. The topics are summarized here:Chapter 1 presents the findings of the study investigating the correlation between structural stability and activity of the antifreeze protein from a desert beetle (Anatolica polita), Trx-ApAFP752. The Varga group has previously structurally characterized ApAFP752 using nuclear magnetic resonance (NMR) spectroscopy, that revealed its unique fold (i.e., b-helix), commonly found among insect AFPs. Here we demonstrated that Trx-ApAFP752 not only has high thermal stability but can provide cryoprotection during freeze/thaw for a freeze sensitive enzyme, lactose dehydrogenase (LDH).Chapter 2 presents the findings of the study investigating secondary structure and cryoprotection of a truncated antifreeze protein from a broadleaf shrub, Ammopiptanthus nanus, AnAFP-AKseg. Based on the sequence homology, this protein can be classified as a dehydrin-like antifreeze protein. We have shown that AnAFP-AKseg can preserve LDH activity after freeze/thaw.
PDF
