This study addresses three questions: How often and how consistently do predictors for emotion regulation choice occur in daily life? What predicts emotion regulation choice in daily life? How do ...predictors for emotion regulation choice interact in daily life? We examined emotion regulation goals (i.e., prohedonic and social goals), situational factors (i.e., perceived control, expected reoccurrence, and emotional intensity), and emotion regulation strategies (i.e., active coping, distraction, rumination, cognitive reappraisal, and expressive suppression) in negative emotion events. A total of 110 individuals (65% female) participated in an experience sampling study and received beeps, five times a day over the course of 9 days. We used a random intercept model to estimate our results. Emotion regulation goals and situational factors vary strongly in different events within the same person. Emotion regulation strategies, effective in changing the emotional experience, are crucial for prohedonic goals, whereas expressive suppression is important for social goals. Perceived control was positively associated with putatively adaptive strategies. Emotional intensity and expected reoccurrence were negatively associated with putatively adaptive strategies. Emotional intensity was positively associated with putatively maladaptive strategies. Emotion regulation strategies were not associated with the interaction of emotion regulation goals and situational factors. We conclude that emotion regulation goals and situational factors are extremely context-dependent, suggesting that they should be treated as states. Emotion regulation goals appear to have a functional association with strategies for prohedonic and social goals. The associations between situational factors and strategies in daily life appear to be largely different from the results found in the laboratory, emphasizing the importance of experience sampling studies.
The rise in popularity of Chat GPT, an advanced language model that uses deep learning techniques to simulate human-like conversation, has raised concerns about its potential misuse, particularly in ...academic contexts. The present study (N = 283) explored the relationship between personality traits and the intention to use chatbot-generated texts for academic cheating. Among the HEXACO and Dark Triad traits, Honesty-Humility, Conscientiousness, Openness to Experience (all negative), Machiavellianism, narcissism, and psychopathy (all positive) were significant predictor variables. A multiple regression analysis showed that Honesty-Humility had the most robust association with the intention to use chatbot-generated texts for academic cheating. Further analyses on the facet level revealed that the fairness facet of Honesty-Humility was the most predictive, suggesting that individuals high in Honesty-Humility refrain from using chatbot-generated texts for academic cheating as they prioritize fairness over their own interests. Promoting Honesty-Humility and its fairness facet can be a valuable approach to promoting ethical behavior in academic and other contexts.
The small heat shock protein αB-crystallin is an oligomeric molecular chaperone that binds aggregation-prone proteins. As a component of the proteostasis system, it is associated with cataract, ...neurodegenerative diseases, and myopathies. The structural determinants for the regulation of its chaperone function are still largely elusive. Combining different experimental approaches, we show that phosphorylation-induced destabilization of intersubunit interactions mediated by the N-terminal domain (NTD) results in the remodeling of the oligomer ensemble with an increase in smaller, activated species, predominantly 12-mers and 6-mers. Their 3D structures determined by cryo-electron microscopy and biochemical analyses reveal that the NTD in these species gains flexibility and solvent accessibility. These modulated properties are accompanied by an increase in chaperone activity in vivo and in vitro and a more efficient cooperation with the heat shock protein 70 system in client folding. Thus, the modulation of the structural flexibility of the NTD, as described here for phosphorylation, appears to regulate the chaperone activity of αB-crystallin rendering the NTD a conformational sensor for nonnative proteins.
Many psychological phenomena have a multilevel structure (e.g., individuals within teams or events within individuals). In these cases, the proportion of between-variance to total-variance (i.e., the ...sum between-variance and within-variance) is of special importance and usually estimated by the intraclass coefficient (1) ICC(1). Our contribution firstly shows via mathematical proof that measurement error increases the within-variance, which in turn decreases the ICC(1). Further, we provide a numerical example, and examine the RMSEs, alpha error rates and the inclusion of zero in the confidence intervals for ICC(1) estimation with and without measurement error. Secondly, we propose two corrections i.e., the reliability-adjusted ICC(1) and the measurement model-based ICC(1) that yield correct estimates for the ICC(1), and prove that they are unaffected by measurement error mathematically. Finally, we discuss our findings, point out examples of the underestimation of the ICC(1) in the literature, and reinterpret the results of these examples in the light of our new estimator. We also illustrate the potential application of our work to other ICCs. Finally, we conclude that measurement error distorts the ICC(1) to a non-negligible extent.
The molecular chaperone αB-crystallin, the major player in maintaining the transparency of the eye lens, prevents stress-damaged and aging lens proteins from aggregation. In nonlenticular cells, it ...is involved in various neurological diseases, diabetes, and cancer. Given its structural plasticity and dynamics, structure analysis of αB-crystallin presented hitherto a formidable challenge. Here we present a pseudoatomic model of a 24-meric αB-crystallin assembly obtained by a triple hybrid approach combining data from cryoelectron microscopy, NMR spectroscopy, and structural modeling. The model, confirmed by cross-linking and mass spectrometry, shows that the subunits interact within the oligomer in different, defined conformations. We further present the molecular architectures of additional well-defined αB-crystallin assemblies with larger or smaller numbers of subunits, provide the mechanism how "heterogeneity" is achieved by a small set of defined structural variations, and analyze the factors modulating the oligomer equilibrium of αB-crystallin and thus its chaperone activity.
Mutations in the leucine-rich repeat kinase 2 gene (LRRK2) are associated with familial and sporadic Parkinson's disease (PD). LRRK2 is a complex protein that consists of multiple domains, including ...predicted C-terminal WD40 repeats. In this study, we analyzed functional and molecular features conferred by the WD40 domain. Electron microscopic analysis of the purified LRRK2 C-terminal domain revealed doughnut-shaped particles, providing experimental evidence for its WD40 fold. We demonstrate that LRRK2 WD40 binds and sequesters synaptic vesicles via interaction with vesicle-associated proteins. In fact, a domain-based pulldown approach combined with mass spectrometric analysis identified LRRK2 as being part of a highly specific protein network involved in synaptic vesicle trafficking. In addition, we found that a C-terminal sequence variant associated with an increased risk of developing PD, G2385R, correlates with a reduced binding affinity of LRRK2 WD40 to synaptic vesicles. Our data demonstrate a critical role of the WD40 domain within LRRK2 function.
Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp ...exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of reproducing adults and embryos. X-ray crystallography and electron microscopy revealed that Sip1 exists in a range of well-defined globular assemblies consisting of two half-spheres, each made of dimeric “spokes.” Strikingly, the oligomeric distribution of Sip1 as well as its chaperone activity depend on pH, with a trend toward smaller species and higher activity at acidic conditions such as present in nematode eggs. The analysis of the interactome shows that Sip1 has a specific substrate spectrum including proteins that are essential for embryo development.
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•Sip1 is a pH-regulated, development-specific molecular chaperone•The crystal structure of the Sip1 32-mer reveals a “mammalian-type” sHsp structure•Cryo-EM 3D reconstructions depict pH-dependent structural plasticity•The interactome shows general protein protection and egg-specific functions
Fleckenstein et al. report that the small heat shock protein Sip1 plays an important role as a molecular chaperone during C. elegans development. It is activated at acidic pH in eggs and embryos, and this correlates with changes in its oligomer structure.
Research on bystander intervention has produced a great number of studies showing that the presence of other people in a critical situation reduces the likelihood that an individual will help. As the ...last systematic review of bystander research was published in 1981 and was not a quantitative meta-analysis in the modern sense, the present meta-analysis updates the knowledge about the bystander effect and its potential moderators. The present work (a) integrates the bystander literature from the 1960s to 2010, (b) provides statistical tests of potential moderators, and (c) presents new theoretical and empirical perspectives on the novel finding of non-negative bystander effects in certain dangerous emergencies as well as situations where bystanders are a source of physical support for the potentially intervening individual. In a fixed effects model, data from over 7,700 participants and 105 independent effect sizes revealed an overall effect size of g = -0.35. The bystander effect was attenuated when situations were perceived as dangerous (compared with non-dangerous), perpetrators were present (compared with non-present), and the costs of intervention were physical (compared with non-physical). This pattern of findings is consistent with the arousal-cost-reward model, which proposes that dangerous emergencies are recognized faster and more clearly as real emergencies, thereby inducing higher levels of arousal and hence more helping. We also identified situations where bystanders provide welcome physical support for the potentially intervening individual and thus reduce the bystander effect, such as when the bystanders were exclusively male, when they were naive rather than passive confederates or only virtually present persons, and when the bystanders were not strangers. (Contains 2 figures, 14 footnotes and 6 tables.)
In recent years, there has been a surge in the quantity of media content that glorifies risk-taking behavior, such as risky driving, extreme sports, or binge drinking. The authors conducted a ...meta-analysis involving more than 80,000 participants and 105 independent effect sizes to examine whether exposure to such media depictions increased their recipients' risk-taking inclinations. A positive connection was found for overall, combined risk taking (g = 0.41); as well as its underlying dimensions: risk-taking behaviors (g = 0.41), risk-positive cognitions and attitudes (g = 0.35), and risk-positive emotions (g = 0.56). This effect was observed across varying research methods (experimental, correlational, longitudinal); types of media (video games, movies, advertising, TV, music); and differing risk-related outcome measures (e.g., smoking, drinking, risky driving, sexual behavior). Multiple moderator analyses revealed 2 theoretically new boundary conditions for sociocognitive models. First, the effect was stronger for active (i.e., video games) than for passive (e.g., film, music) exposure to risk-glorifying media content. Second, the effect was stronger when there was a high degree of contextual fit between the media content and type of risk-taking measure. The theoretical, practical, and societal implications of the present research synthesis are discussed. (Contains 1 figure, 7 tables and 6 footnotes.)
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