CD14 is a co-receptor involved in the recognition of Gram-negative and Gram-positive bacteria, the latter known to cause dental caries. The aim of this study was to determine whether soluble CD14 ...(sCD14) in saliva was associated with caries activity and the collection method from the saliva. The study included 55 participants aged 20 to 40 years, 30 with dental caries and 25 caries-free controls. We collected 110 saliva samples in total, 55 of resting saliva and 55 of mechanically stimulated saliva. Median levels of sCD14, measured with a matrix-matched enzyme-linked immunosorbent assay (ELISA), were higher in the caries-active than in the caries-free group in either resting (203.3 vs.167.9 ng mL
; P<0.01) or stimulated saliva (201 vs.105.7 ng mL
; P<0.01). The resting salivary flow rate was lower in the caries-active than caries-free group (0.61±0.42 vs. 0.98±0.52 mL min-
; P<0.01). Hyposalivation was observed only in the caries-active group (10 and 13 % in stimulated and resting saliva, respectively). Higher salivary sCD14 levels and secretion rates were clearly associated with dental caries and resting saliva. Future studies should focus on the clinical utility of salivary sCD14 as a potential biomarker and predictor of future caries events.
The aim of this study was to evaluate
the antibacterial effect of ozone on suspension of three different bacteria inoculated in prepared canals of extracted human teeth.
Ozone was produced by special ...KP syringe of high frequency ozone generator Ozonytron (Biozonix, München, Germany) from aspirated atmospheric air by dielectric barrier discharge and applied through the tip of the syringe to the prepared root canal. The microorganisms used were
,
and
.
However, none of the methods was 100% effective against the three bacterial types in suspension. Application of ozone significantly decreased the absolute count of microorganisms (89.3%), as well as the count of each type of bacteria separately (
94.0%;
88.6% and
79.7%). Ozone generated by KP syringe was statistically more effective compared to NaOCl as positive control, for
and
.
The absolute count of
was statistically decreased without a statistically significant difference between the tested group and positive control, respectively. Among the three types of bacteria in suspension, KP probe had the lowest antimicrobial effect against
.
Erosive lesions in patients with alcoholism Dukić, Walter; Dobrijević, Tanja Trivanović; Katunarić, Marina ...
The Journal of the American Dental Association (1939),
12/2010, Letnik:
141, Številka:
12
Journal Article
Recenzirano
The authors conducted a study to determine the occurrence of erosive tooth lesions in patients with alcoholism and to establish the influence of salivary flow rates and pH levels on their appearance.
...The authors conducted a cross-sectional study in 140 participants (70 with clinically diagnosed alcoholism who were undergoing therapy for their addiction were in the test group and 70 who did not consume alcohol were in the control group). The authors determined the participants' salivary statuses by measuring the flow rates and pH levels of both unstimulated and stimulated saliva.
The authors found more erosive lesions in the test group (P < .01). They detected a higher number of erosive lesions in participants in the test group who had a pH range of 5 to 6 compared with a pH range of 6 to 7 (P = .01). They found a significant correlation between alcoholism and unstimulated salivary flow rate (P < .05).
The salivary flow rate was similar in control and test groups. The prevalence of erosion in the test group was higher than that in the control group, which may be related to the decrease in salivary pH of both stimulated and unstimulated saliva in this group. The results of the study showed no connection between erosion prevalence and pH levels and stimulated salivary flow rates.
Patients with alcoholism may be at risk of developing erosive lesions on their teeth owing to the low pH level of their oral environment and decreased saliva levels.
The aim of the study was to determine the prevalence of caries in patients who are heroin addicts and the effects of salivary flow and saliva pH on its prevalence. The study included 200 subjects ...(100 subjects diagnosed with heroin addiction as the test group and 100 subjects taking no addictive substances as the control group). In both groups, saliva status was established by determining flow rates and pH of stimulated and unstimulated saliva. The total number of dental caries cases was determined using the DMFT index and their more specific detection in the context of localization on the tooth using the DMFS index. The DMFT index was higher in the test group (mean 18.78) than in the control group (mean 5.32), with a statistically significant difference (P < 0.000). The highest caries prevalence in both groups was recorded on occlusal dental surfaces (44.70% in the test group and 16.20% in the control group). The prevalence of caries of vestibular dental surfaces was higher in the test group (9.40%) than in the control group (2.34%), and the difference was statistically significant (P < 0.000). A statistically significant correlation was established between heroin addiction and reduced production of unstimulated saliva (P < 0.001) and stimulated saliva (P < 0.002), as well as pH values of stimulated saliva (P < 0.001) and unstimulated saliva (P < 0.001). In conclusion, a higher prevalence of caries of hard dental tissue was found in the test group. The flow of stimulated and unstimulated saliva as was as saliva pH was lower in the test group.
Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to ...evaluate the genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in dimethyl sulfoxide for 1 hour, 24 h and 120 h (5 days). Thereafter, lymphocyte cultures were treated with different concentrations of eluates (0.2 microg/mL, 0.5 microg/mL and 5 microg/mL) obtained from each of the tested materials. Genotoxicity was evaluated by micronucleus test. The chi2-test was used on statistical analysis (p < 0.05). After elution period of 1 h, only the highest dose of all tested materials affected the measured cytogenetic parameters. After 24 h, genotoxicity was demonstrated only in cultures treated with eluates in concentrations of 0.5 microg/mL and 5 microg/mL. Based on the results, it is concluded that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect of these dental adhesives increases with the tested material concentration and decreases with the length of elution period.
The purpose of this in vitro study was to evaluate microleakage along glass-fibre posts cemented with three different cements after cyclic loading. After post-space preparation, fifty obturated root ...canals were randomly divided into three experimental groups and two control groups. In group 1, Glassix posts were cemented using Harvard cement, in group 2, Fuji PLUS cement was used and in group 3, Variolink II was used for post cementation. The specimens were artificially aged by loading in a special testing machine. Coronal leakage was evaluated using a fluid transport system. Posts cemented with Variolink II, showed significantly higher failure rate after loading, compared to group 1 and 2 (p = 0.009). Comparing microleakage in samples that have not failed, specimens cemented with Variolink II showed significantly less fluid transport than specimens cemented with zinc phosphate and glass ionomer cements (p = 0.04 and p = 0.006, respectively). Variolink II cement exibited significantly less fluid movement compared with Harvard and Fuji PLUS cement.
The purpose of this study was to determine the prevalence of third molars in terms of respondents' age. Furthermore it was desired to determine the extent of the manifestation of pathological ...changes, such as caries, apical lesions, and alveolar bone resorption. This study was based on 500 OPGs of patients from the Faculty of Dentistry, University of Zagreb. Patients were divided into seven age groups. The total number of molars, the number of cavities on remaining molars, the quality of endodontic treatment and the evaluation of their performance and presence of periapical lesions was analyzed. As apart of the periodontal status, the number of inadequate fillings, the number of damaged furcations and bone resorption was analyzed. The statistical analysis was performed by means of STATISTICA 7 statistical package (StatSoft, Tulsa, USA). The correlation between the total number of molars and the age of patients was analyzed by means of Spearman's correlation to the level of significance of 0.05. The number of first, second and third molars significantly decreased in higher age groups. The prevalence of caries in third molars as compared to first and second molars was generally lower, third molars were endodontic treated at least. Assessment of involved furcations showed that the lowest percentage of 8% was found in third molars. The majority of all remaining molars had bone resorption of 1-3 mm.
Eighty dentate students participated as a study group and another 74 as a control group. The aim was to determine a possibility to reconstruct maxillary frontal teeth dimensions by use of certain ...hard palate dimensions. The height (IH) and the incisal (IW), contact point (CtW) and cervical width (CW) of maxillary central incisors (MCI), hamular width (HW) and the distance between the incisive papilla and the palatine foveas (IP-FP) were measured on the maxillary casts. CtW of maxillary lateral incisors and canines were measured too. In the study group the ratios were computed: HW/IW (5.71), HW/CtW (5.69), HW/CW (5.51) and IP-FP/IH (4.76). These ratios were multiplied by incisor's dimensions (obtained from the control group) to calculate the hard palate dimensions. No significant differences were obtained between the calculated and the measured (study group) hard palate dimensions. Furthermore, there was no significant difference between the HW and the Sum of contact-point widths of all maxillary frontal teeth (p > 0.05) in the both groups. The results revealed: 1. MCI width and height might be calculated by dividing dimensions of a patient's hard palate and appropriate ratio; 2. hamular width dimension can be used as a selection guide for the sum of contact-point widths of six maxillary frontal teeth.
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