Objective
This study examines the determinants of marriage decline in South Korea, a representative case of the “demographic crisis” sweeping East Asia.
Background
The major theories accounting for ...marriage and family trends are for the most part based on Western cases. A complementing focus on non‐Western societies is likely to identify a more diverse range of processes governing marriage patterns in advanced capitalist societies.
Method
The study draws on the Korean Labor and Income Panel Study (KLIPS) to analyze a sample of 4201 unmarried individuals whose longitudinal data were organized into 55,989 person‐year records. Discrete‐time hazard models incorporating 23 waves of KLIPS data (1998–2020) identify the gendered determinants of marriage.
Results
Socioeconomic resources continue to positively impact men's marriage chances although income, relative to employment status and educational attainment, has become paramount for members of the younger 1980s cohort. Parental wealth, an important precondition for home purchases, also positively impacts the likelihood of marriage for men. Income and parental wealth have become important for women as well but unlike the documented “educational crossover” that has occurred elsewhere, high educational attainment remains negatively associated with marriage probability for Korean women.
Conclusion
This study clarifies the scope conditions for arguments about the “shifting economic foundations of marriage,” while foregrounding the enduring legacy of extended‐family resources in strong familism societies. The results also lend empirical leverage to past studies highlighting the clear disincentives for marriage among highly educated women and provide a more comprehensive picture of why underprivileged men are being left out of Korea's marriage market.
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•HFD feeding led to obesity, memory impairment, and anxiety-like behaviors in mice.•HFD feeding increased Proteobacteria population in gut microbiota and increased fecal and blood LPS ...levels.•HFD feeding suppressed hippocampal BDNF expression and colonic TNFα expression.•HFD-treated mouse stool lysates suppressed BDNF/pCREB expression and increased NF-κB activation in vitro.
The excessive intake of a high-fat diet (HFD) leads to obesity, including metabolic syndromes, disturbs gut microbiota composition, causes colitis, and increases the plasma concentration of lipopolysaccharide (LPS). In the present study, we examined the role of gut microbiota in the occurrence of HFD-induced psychiatric disorders in mice. C57BL/6 J male mice fed a HFD for 9 weeks were led to obesity; their memory impairment was assessed by the Y-maze and novel object recognition test, and anxiety-like behaviors by the elevated plus maze. The intake of a HFD suppressed brain-derived neurotrophic factor (BDNF) expression in the hippocampus and increased blood TNF-α and LPS levels. HFD treatment more potently increased NF-κB activation and Iba1+ (microglial) cell populations in the hippocampus. Furthermore, HFD feeding increased TNF-α expression, myeloperoxidase activity, and CD11b+/CD11c+ cell (macrophages and dendritic cells) populations in the colon and altered gut microbiota composition including increases in the Proteobacteria population, and increases in fecal LPS levels. The stool lysates of HFD-treated mice suppressed BDNF expression and CREB phosphorylation in SH-SY5Y cells and increased NF-κB activation in BV-2 microglial cells compared to those of low-fat diet-treated mice while these effects were attenuated by treatment with anti-LPS antibody. These findings suggest that excessive intake of HFD can simultaneously cause obesity and psychiatric disorders by suppressing hippocampal BDNF expression with the disturbance of gut microbiota composition, particularly the increase in Proteobacteria population and LPS production.
This study examines current household disaster preparedness and identifies its predictors in South Korea. A structured online survey was administered to 1,243 participants quota-sampled by age and ...population from each administrative district. Based on the socio-ecological model, interpersonal factors (general characteristics, prior disaster experience, anxiety, dispositional optimism, perceived disaster risk, and disaster preparedness knowledge), institutional factor (front-line preparedness), community factor (community resilience), public policy factor (governmental preparedness), and household disaster preparedness were measured. The data were analyzed using descriptive statistics, Mann–Whitney U test, Kruskal–Wallis test, Bonferroni test, Pearson’s correlation coefficients, and multiple regression. The predictors of household disaster preparedness were occupation, economic status, prior disaster experience, anxiety, disaster preparedness knowledge, front-line preparedness, and community resilience. The most potent predictor of household disaster preparedness was community resilience. Our finding that community resilience, a community factor, has a greater impact on household disaster preparedness than personal factors calls for programs that promote such resilience. Further, continuous public education and campaigns are needed to increase public awareness of household disaster preparedness and to improve the public’s competency to prepare for potential disasters. This study raises the need for community programs for residents to increase household disaster preparedness knowledge and improve their competencies related to disaster response. This study is significant in highlighting the importance of community factors in improving household disaster preparedness amid the need to prepare for various types of disasters.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
DNAJB9, a member of the heat shock protein 40 family, acts as a multifunctional player involved in the maintenance of their client proteins and cellular homeostasis. However, the mechanistic action ...of DNAJB9 in human malignancies is yet to be fully understood. In this study, we found that ectopic restoration of DNAJB9 inhibits the migration, invasion, in vivo metastasis, and lung colonization of triple-negative breast cancer (TNBC) cells. Mechanistically, DNAJB9 stabilizes FBXO45 protein by suppressing self-ubiquitination and reduces the abundance of ZEB1 by Lys48-linked polyubiquitination to inhibit the epithelial-mesenchymal transition (EMT) and metastasis. Clinically, the reduction of DNAJB9 expression, concomitant with decreased FBXO45 abundance in breast cancer tissues, correlates with poorer clinical outcomes of patients with breast cancer. Taken together, our results provide a novel insight into the metastasis of TNBC and define a promising therapeutic strategy for cancers with overactive ZEB1 by regulating the DNAJB9-FBXO45 signaling axis.
Akkermansia muciniphila has received great attention because of its beneficial roles in gut health by regulating gut immunity, promoting intestinal epithelial development, and improving barrier ...integrity. However, A. muciniphila-derived functional molecules regulating gut health are not well understood. Microbiome-secreted proteins act as key arbitrators of host-microbiome crosstalk through interactions with host cells in the gut and are important for understanding host-microbiome relationships. Herein, we report the biological function of Amuc_1409, a previously uncharacterised A. muciniphila-secreted protein. Amuc_1409 increased intestinal stem cell (ISC) proliferation and regeneration in ex vivo intestinal organoids and in vivo models of radiation- or chemotherapeutic drug-induced intestinal injury and natural aging with male mice. Mechanistically, Amuc_1409 promoted E-cadherin/β-catenin complex dissociation via interaction with E-cadherin, resulting in the activation of Wnt/β-catenin signaling. Our results demonstrate that Amuc_1409 plays a crucial role in intestinal homeostasis by regulating ISC activity in an E-cadherin-dependent manner and is a promising biomolecule for improving and maintaining gut health.
Poly (ADP‐ribose) polymerase 1 (PARP1) and polycomb repressive complex 2 (PRC2) have important individual roles in the development of cancer. In BRCA‐mutant breast cancers, research on the ...co‐operation of these two factors has focused on the DNA damage repair process; however, how they cooperate to modulate the tumor microenvironment remains unclear. Here, Mi‐Young Kim and colleagues show that simultaneous inhibition of PARP1 and PRC2 in BRCA‐proficient triple negative breast cancer leads to enhanced angiogenesis and macrophage differentiation. The authors reveal a previously unknown synthetic viable interaction between PARP1 and PRC2 that results in a NF‐κB‐mediated modification of the tumor microenvironment.
Poly (ADP‐ribose) polymerase 1 (PARP1) and polycomb‐repressive complex 2 (PRC2) are each known for their individual roles in cancer, but their cooperative roles have only been studied in the DNA damage repair process in the context of BRCA‐mutant cancers. Here, we show that simultaneous inhibition of PARP1 and PRC2 in the MDA‐MB‐231 BRCA‐proficient triple‐negative breast cancer (TNBC) cell line leads to a synthetic viability independent of the mechanisms of DNA damage repair. Specifically, we find that either genetic depletion or pharmacological inhibition of both PARP1 and PRC2 can accelerate tumor growth rate. We attribute this to modifications in the tumor microenvironment (TME) that are induced by double‐depleted breast cancer cells, such as promoting intratumoral angiogenesis and increasing the proportion of tumor‐promoting type 2 (M2) macrophages. These changes subsequently inhibit cell death and promote proliferation. Mechanistically, we find that PARP1 and PRC2 double depletion induces not only a basal activation of the NF‐κB pathway but also a maximal activation of NF‐κB within the TME in response to external stimuli such as hypoxia and the presence of macrophages. In summary, our study reveals an unprecedented synthetic viable interaction between PARP1 and PRC2 in BRCA‐proficient TNBC and identifies NF‐κB as the downstream mediator.
Database
RNA‐seq data are available in the GEO databases under the accession GSE142769.
MicroRNAs (miRNAs) constitute a class of small noncoding RNAs that play important roles in a variety of biological processes including development, apoptosis, proliferation, and differentiation. Here ...we show that the expression of miR-199a and miR-199a*(miR-199a/a*), which are processed from the same precursor, is confined to fibroblast cells among cultured cell lines. The fibroblast-specific expression pattern correlated well with methylation patterns: gene loci on chromosome 1 and 19 were fully methylated in all examined cell lines but unmethylated in fibroblasts. Transfection of miR-199a and/or -199a* mimetics into several cancer cell lines caused prominent apoptosis with miR-199a* being more pro-apoptotic. The mechanism underlying apoptosis induced by miR-199a was caspase-dependent, whereas a caspase-independent pathway was involved in apoptosis induced by miR-199a* in A549 cells. By employing microarray and immunoblotting analyses, we identified the MET proto-oncogene as a target of miR-199a*. Studies with a luciferase reporter fused to the 3′-untranslated region of the MET gene demonstrated miR-199a*-mediated down-regulation of luciferase activity through a binding site of miR-199a*. Interestingly, extracellular signal-regulated kinase 2 (ERK2) was also down-regulated by miR-199a*. Coordinated down-regulation of both MET and its downstream effector ERK2 by miR-199a* may be effective in inhibiting not only cell proliferation but also motility and invasive capabilities of tumor cells.
Conventional magnetophoresis techniques for manipulating biocarriers and cells predominantly rely on large‐scale electromagnetic systems, which is a major obstacle to the development of portable and ...miniaturized cell‐on‐chip platforms. Herein, a novel magnetic engineering approach by tailoring a nanoscale notch on a disk micromagnet using two‐step optical and thermal lithography is developed. Versatile manipulations are demonstrated, such as separation and trapping, of carriers and cells by mediating changes in the magnetic domain structure and discontinuous movement of magnetic energy wells around the circumferential edge of the micromagnet caused by a locally fabricated nano‐notch in a low magnetic field system. The motion of the magnetic energy well is regulated by the configuration of the nanoscale notch and the strength and frequency of the magnetic field, accompanying the jump motion of the carriers. The proposed concepts demonstrate that multiple carriers and cells can be manipulated and sorted using optimized nanoscale multi‐notch gates for a portable magnetophoretic system. This highlights the potential for developing cost‐effective point‐of‐care testing and lab‐on‐chip systems for various single‐cell‐level diagnoses and analyses.
A sophisticated engineering approach involving a tailored nano‐micro scale notched micromagnet is a novel gate that efficiently enables versatile cell manipulation, such as separation and capture, with a compact system area and less power for operation. This technique provides a milestone for a simple and effective solution for a portable cell‐on‐chip platform.
To evaluate not only the risk of total preterm birth (PTB) but also spontaneous preterm birth (sPTB) and indicated preterm birth (iPTB) in vanishing twin (VT).
This is a secondary analysis of a ...multicenter prospective cohort study. In 12 different healthcare institutions, women with singleton pregnancies were enrolled in early pregnancy and followed up till delivery.
A total of 4,746 women were included in the final analysis, and. the frequency of VT was 1.1% (54/4746). VT group had a higher risk for total PTB (PTB<34 weeks, 2.1% vs. 14.8%, p<0.001; PTB<32 weeks, 1.6% vs. 13.0%, p<0.001; PTB<28 weeks, 0.9% vs. 13.0%, p<0.001) than singleton group. The VT group had increased risk for both sPTB and iPTB (<34 weeks, <32 weeks, and <28 weeks), and this increased risk for sPTB and iPTB in VT group remained significant even after controlling for confounders such as maternal age, parity, pre-pregnancy BMI, and mode of conception.
Vanishing twin can be an independent risk factor for both sPTB and iPTB when compared with singleton pregnancy.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Memory T cell responses have been demonstrated in COVID-19 convalescents, but ex vivo phenotypes of SARS-CoV-2-specific T cells have been unclear. We detected SARS-CoV-2-specific CD8+ T cells by MHC ...class I multimer staining and examined their phenotypes and functions in acute and convalescent COVID-19. Multimer+ cells exhibited early differentiated effector-memory phenotypes in the early convalescent phase. The frequency of stem-like memory cells was increased among multimer+ cells in the late convalescent phase. Cytokine secretion assays combined with MHC class I multimer staining revealed that the proportion of interferon-γ (IFN-γ)-producing cells was significantly lower among SARS-CoV-2-specific CD8+ T cells than those specific to influenza A virus. Importantly, the proportion of IFN-γ-producing cells was higher in PD-1+ cells than PD-1− cells among multimer+ cells, indicating that PD-1-expressing, SARS-CoV-2-specific CD8+ T cells are not exhausted, but functional. Our current findings provide information for understanding of SARS-CoV-2-specific CD8+ T cells elicited by infection or vaccination.
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•SARS-CoV-2-specific CD8+ T cells are effector memory cells in convalescents•CCR7+CD45RA+ cells are increased among SARS-CoV-2-specific cells in the late phase•SARS-CoV-2-specific CD8+ T cells have fewer IFN-γ+ cells than flu-specific cells•PD-1-expressing SARS-CoV-2-specific CD8+ T cells are not exhausted but functional
T cell responses have been demonstrated in COVID-19 patients, but ex vivo phenotypes and functions of SARS-CoV-2-specific T cells remain unclear. Rha et al. examined SARS-CoV-2-specific CD8+ T cells in acute and convalescent COVID-19 patients using MHC class I multimers, finding that PD-1-expressing SARS-CoV-2-specific CD8+ T cells are not exhausted but functional.
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