The identification of somatic
variation is crucial to confirm the heritability of retinoblastoma. We and others have previously shown that, when tumour DNA is unavailable, cell-free DNA (cfDNA) ...derived from aqueous humour (AH) can be used to identify somatic
pathogenic variation. Here we report
pathogenic variant detection, as well as cfDNA concentration in an extended cohort of 75 AH samples from 68 patients. We show cfDNA concentration is highly variable and significantly correlated with the collection point of the AH. Cell-free DNA concentrations above 5 pg/µL enabled the detection of 93% of known or expected
pathogenic variants. In AH samples collected during intravitreal chemotherapy treatment (Tx), the yield of cfDNA above 5 pg/µL and subsequent variant detection was low (≤46%). However, AH collected by an anterior chamber tap after one to three cycles of primary chemotherapy (Dx1+) enabled the detection of 75% of expected pathogenic variants. Further limiting our analysis to Dx1+ samples taken after ≤2 cycles (Dx ≤ 2) provided measurable levels of cfDNA in all cases, and a subsequent variant detection rate of 95%. Early AH sampling is therefore likely to be important in maximising cfDNA concentration and the subsequent detection of somatic
pathogenic variants in retinoblastoma patients undergoing conservative treatment.
This thesis explores the relationship between performance and consumption in relation to play collection in the 1630s, and also examines the wider contexts of performance and consumption in that ...decade. It proposes that the 1630s were a decade characterised by particularly self-conscious performances of consumption, and that this environment contributed directly to the beginnings of the collection of books for display purposes. A focus on the Petworth collection and its original collector is maintained throughout the thesis, which weaves together the material and literary content of the collection. Using material evidence from the volumes themselves, this thesis demonstrates that the collection was purchased in 1638 by the 10th Earl of Northumberland through an agent who assembled the collection specifically for the Earl just prior to his purchase of it. It also demonstrates, again using evidence from the volumes themselves, that the purchase was partly informed by principles of education, personal taste and a consideration for family history, but that the overwhelming motive was the drive to consume and to perform that consumption. Using the literary content of the collection to explore representations of performed consumption, this thesis tracks the development of the conceptualisation of consumption on the stage from the wariness about dangerous consumption in the late Elizabethan period to the much more open, and yet still rather complex, attitudes of the 1630s. Finally, the thesis discusses some other kinds of public, performed consumption, including a procession by Northumberland and an entertainment with which he was connected, exploring the explicitly social elements of performance. The Petworth play collection is at once anomalous and typical as an example of mid-17th century book collection, and it can be used to illustrate and map the multitude of issues, concepts and attitudes which surround performance, consumption and collection in the 1630s, and beyond.
This thesis explores the relationship between performance and consumption in relation to play collection in the 1630s, and also examines the wider contexts of performance and consumption in that ...decade. It proposes that the 1630s were a decade characterised by particularly self-conscious performances of consumption, and that this environment contributed directly to the beginnings of the collection of books for display purposes. A focus on the Petworth collection and its original collector is maintained throughout the thesis, which weaves together the material and literary content of the collection. Using material evidence from the volumes themselves, this thesis demonstrates that the collection was purchased in 1638 by the 10th Earl of Northumberland through an agent who assembled the collection specifically for the Earl just prior to his purchase of it. It also demonstrates, again using evidence from the volumes themselves, that the purchase was partly informed by principles of education, personal taste and a consideration for family history, but that the overwhelming motive was the drive to consume and to perform that consumption. Using the literary content of the collection to explore representations of performed consumption, this thesis tracks the development of the conceptualisation of consumption on the stage from the wariness about dangerous consumption in the late Elizabethan period to the much more open, and yet still rather complex, attitudes of the 1630s. Finally, the thesis discusses some other kinds of public, performed consumption, including a procession by Northumberland and an entertainment with which he was connected, exploring the explicitly social elements of performance. The Petworth play collection is at once anomalous and typical as an example of mid-17th century book collection, and it can be used to illustrate and map the multitude of issues, concepts and attitudes which surround performance, consumption and collection in the 1630s, and beyond.
The book highlights many interesting discussion areas, leading toward reflection led practice. The need and value of supervision for those working for the NHS is another interesting area touched on ...throughout this book' \- A 'It is recommended for all counsellors who work part-time or full-time in healthcare, and will also be invaluable for many colleagues of these counsellors and also all those who manage such services. Each chapter is written in a lively, personal way that invites the reader into a relationship with the author, raising awareness of the dilemmas counsellors may face in their work and what they need to support them, especially training and good supervision. The book represents a significant step forward in the literature on healthcare counselling and a copy should find its way into every healthcare library where counselling is a key part of the delivery of care.' \- Healthcare Counselling and Psychotherapy Journal 'It is recommended for all counsellors who work part-time or full-time in health-care, and will also be invaluable for many colleagues of these counsellors and also all those who manage such services. Each chapter is written in a lively, personal way that invites the reader into a relationship with the author, raising awareness of the dilemmas counsellors may face in their work and what they need to support them, especially training and good supervision. The book represents a significant step forward in the literature on healthcare counselling and a copy should find its way into every healthcare library where counselling is a key part of the delivery of care.' \- HCPJ 'This thought-provoking book gives contextualisation to the growth area of counselling in the NHS and elsewhere. Each chapter provides fascinating personal insights into experiences such as palliative care, counselling, cancer care, living with HIV/AIDS, young people and sexual health, counselling in fertility problems and two chapters on counselling in the mental health services. It explores the lived experiences of clients and professionals, helping them to apply theory to practice and giving, rather than detached observations, intensely personal windows into what actually goes on between client and counsellor. Despite being centred mostly on hospital work, the book would be useful for work in the domiciliary setting, particularly for community psychiatric nurses, school nurses and for those who specialise in serious, chronic, or life-threatening conditions.' \- Community Practitioner 'I very much enjoyed reading this book because of the insight it gave me into the different ways in which counsellors work in health settings. Such information can be difficult to come by because of confidentiality issues and when working with individual clients. I was also touched by the open way in which all the authors wrote when including personal details and their own reflections on their work. Throughout this book I found my attention held by the counsellors' accounts of the way in which they approached their work with clients, linking with their personal reflections and comments on their own theoretical perspectives. Each chapter is written by a different author and includes queries and questions raised by the difficulties in the work they undertake, such as balancing the medical model with a person-centred holistic model, confidentiality issues, and the importance of supervision when working with very distressed clients. I recommend this book to all counsellors wishing to gain some insight into counselling in different settings' \- National Association of Cancer Counsellors Focusing on the experiences of counsellors themselves, this book is a comprehensive resource for counsellors working in health contexts and for the health professionals who work with them, and may take on counselling roles. The contributors, who include clients, health care workers, psychiatrists and academics as well as counsellors, show how counselling can and should form an integral part of a patient's health care, explaining key theoretical considerations and relevant research and applying these to suggestions for good practice. They also discuss the needs of counsellors themselves (for example for supervision and support) and the variety of roles that counsellors and health care workers are expected to fulfil. This book will inform and improve the work of professionals in a range of health care contexts including those working with patients who have cancer, HIV and AIDS or who are having treatment for infertility; and in the fields of young people's sexual health and palliative care.
The purpose of this study was to determine the effects that a pre-laboratory guide, conceptualized as a “scientific story grammar,” has on college chemistry students' learning when they read an ...introductory chemistry laboratory manual and perform the experiments in the chemistry laboratory. The participants (N = 56) were students enrolled in four existing general chemistry laboratory sections taught by two instructors at a women's liberal arts college. The pre-laboratory guide consisted of eight questions about the experiment, including the purpose, chemical species, variables, chemical method, procedure, and hypothesis. The effects of the intervention were compared with those of the traditional pre-laboratory assignment for the eight chemistry experiments. Measures included quizzes, tests, chemistry achievement test, science process skills test, laboratory reports, laboratory average, and semester grade. The covariates were mathematical aptitude and prior knowledge of chemistry and science processes, on which the groups differed significantly. The study captured students' perceptions of their experience in general chemistry through a survey and interviews with eight students. The only significant differences in the treatment group's performance were in some subscores on lecture items and laboratory items on the quizzes. An apparent induction period was noted, in that significant measures occurred in mid-semester. Voluntary study with the pre-laboratory guide by control students precluded significant differences on measures given later in the semester. The groups' responses to the survey were similar. Significant instructor effects on three survey items were corroborated by the interviews. The researcher's students were more positive about their pre-laboratory tasks, enjoyed the laboratory sessions more, and were more confident about doing chemistry experiments than the laboratory instructor's groups due to differences in scaffolding by the instructors.
The purpose of the study was to determine the educational soundness of selected social studies instructional materials being used in Filipino bilingual programs at the elementary level in California. ...The statewide survey of social studies instructional materials conducted by the researcher revealed that only levels one and two of those produced by the Asian American Bilingual Center (AABC) were accompanied by teacher's guides and were the most widely used; therefore, these were selected for the study. Sections of the Social Science Education Consortium Ethnic Heritage Studies Materials Analysis Instrument dealing with educational soundness were used by the researcher and two other independent judges in analyzing the content of the AABC materials. According to the results of the content analysis, the strength of the materials lies in their adaptability to conditions relating to pupils, teachers, general curricula, schools, and community. The materials were found to be directed towards the Filipino, Chinese, and Japanese ethnic groups, and if used alone, would present a slightly distorted ethnic heritage curriculum. The materials also show a balance between cognitive objectives which were rated as clear, and affective objectives which were judged to be fairly clear. It was also found that the substantive content and instructional methods used were somewhat innovative in level one and slightly traditional in level two materials. Improvement was needed in the areas of instructional theory and rationale. This study revealed the need to develop and analyze more social studies instructional materials for Filipino bilingual programs at the elementary level. Materials for other linguistic groups should be analyzed to determine their educational soundness, ethnic accuracy, and appropriateness for the target populations whose learning styles are influenced by their cultures. It is strongly recommended that educational theorists be consulted in the planning, development, and evaluation of social studies instructional materials.
The aim of this book is to present review articles describing the latest theoretical and experimental developments in the field of cold atoms and molecules. Our hope is that this series will promote ...research by both highlighting recent breakthroughs and by outlining some of the most promising research directions in the field.
