High density lipoprotein (HDL) as well as annexin A1 have been reported to be associated with cardiovascular protection. However, the correlation between HDL and annexin A1 was still unknown. In this ...study, HDL increased endothelial annexin A1 and prevented the decrease of annexin A1 in TNF-α-activated endothelial cells in vitro and in vivo, and above effects were attenuated after knockdown of annexin A1. Annexin A1 modulation affected HDL-mediated inhibition of monocyte adhesion to TNF-α-activated endothelium (45.2±13.7% decrease for annexin A1 RNA interference; 78.7±16.3% decrease for anti-Annexin A1 antibody blocking; 11.2±6.9% increase for Ad-ANXA1 transfection). Additionally, HDL up-regulated annexin A1 through scavenger receptor class B type I, involving ERK, p38MAPK, Akt and PKC signaling pathways, and respective inhibitors of these pathways attenuated HDL-induced annexin A1 expression as well as impaired HDL-mediated inhibition of monocyte–endothelial cell adhesion. Apolipoprotein AI also increased annexin A1 and activated similar signaling pathways. Endothelial annexin A1 from apolipoprotein AI knockout mice was decreased in comparison to that from wild type mice. Finally, HDL-induced annexin A1 inhibited cell surface VCAM-1, ICAM-1 and E-selectin, and secretion of MCP-1, IL-8, VCAM-1 and E-selectin, thereby inhibiting monocyte adhesion.
•HDL up-regulated endothelial annexin A1 via SR-BI and activation of ERK, p38MAPK, Akt and PKC signaling pathways.•Endothelial ANXA 1 inhibited monocyte adhesion.•High density lipoprotein-induced annexin A1 not only inhibited cell surface VCAM-1, ICAM-1, and E-selectin but also suppressed secretion of MCP-1, IL-8, VCAM-1 and E-selectin, which alleviated inflammatory response.
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Developing metal-free catalysts for various applications has been the focus of high interest over the past decade, especially aiming to replace the expensive noble metal-based ...catalysts. Herein, a well-defined three-dimensional nitrogen-doped graphene foam (3D-NGF) is synthesized and employed as a metal-free catalyst for the hydrogenation reduction of p-Nitrophenol to p-Aminophenol. The apparent activation energy is calculated, and the reaction mechanism with 3D-NGF as the catalyst for the hydrogenation reduction of p-Nitrophenol is proposed. Importantly, the 3D-NGF demonstrates high catalytic activity and robust stability. The high activity can be ascribed to the synergistic effect between the nitrogen-doping induced change in electronic property and the 3D foam-like structure.
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•The magnetic AC/Fe3O4/UiO-66 exhibited a high adsorption capacity of 85.9 μg/g for elemental mercury.•The porous nanocomposites have good stability and magnetism in ...mercury-containing flue gas.•The nanocomposites show excellent regeneration and magnetic separation performance.
Activated carbon injection technology has been identified as the leading method for removing elemental mercury (Hg0) in coal-fired power plants. However, this technology can only achieve pollutant transfer, with the disposal of removal products and the inability to recycle adsorbents, and there is an issue of secondary pollutant release. In this work, a series of magnetic composites AC/Fe3O4/UiO-66-X were prepared by adding different amounts of activated carbon during the synthesis process. Activated carbon was introduced into the synthesis process of magnetic metal–organic framework Fe3O4/UiO-66 to enhance its dispersibility and improve its adsorption capacity of Hg0 in simulated flue gas. The porous composites have good stability and magnetism. The elemental mercury removal experiment showed that the addition of activated carbon greatly enhanced the removal performance in the simulated flue gas, with a maximum adsorption capacity was 85.9 μg/g. The spent magnetic composite AC/Fe3O4/UiO-66–1.0 was recycled, and the results showed that the adsorbent remained good mercury-removal performance after four cycles of regeneration. The regenerated adsorbent showed an 8.4 % decrease compared to the fresh adsorbent after four times of recycling. The recovery rate can even exceed 65 % at the high wind speed of 9 m/s in the wind tunnel with magnetic field.
Through the shake-flask method, the solubility of p-nitrobenzamide in 12 pure solvents including n-propanol, ethanol, isopropanol, n-butanol, water, isobutanol, ethyl acetate, acetonitrile, dimethyl ...sulfoxide (DMSO), N,N-dimethylformamide (DMF), N-methyl-2-pyrrolidinone (NMP), and ethylene glycol (EG) was acquired over a temperature range from 283.15 to 328.15 K at ambient pressure p = 101.2 kPa. The mole fractions of p-nitrobenzamide in the equilibrium liquid phase increased as the temperature increased and had the following order in various solvents: DMSO > DMF > NMP > EG > ethyl acetate > ethanol > isopropanol > n-propanol > isobutanol > acetonitrile > n-butanol > water. They were fitted through the Wilson model, modified Apelblat equation, λh equation, and NRTL model. The maximum root-mean-square deviation value and relative average deviation value gained through the four models were, respectively, 56.69 × 10–4 and 6.55 × 10–2. The relative average deviation values achieved were smaller through the modified Apelblat equation than through the other equations for a given solvent. The mixing properties, infinitesimal concentration activity coefficient, and reduced excess enthalpy were derived. Additionally, using the relationship analysis of the Kamlet and Taft linear solvation energy of the solvent effect, the degree and type of interactions of solute–solvent and solvent–solvent were recognized.
AbstractCu+-modified SBA-15 (Cu+/SBA-15, SBA-15 refers well-ordered hexagonal mesoporous silica) was prepared for adsorption of tetracycline (TC) by the pH adjusting reduction method. Some Cu+ ...species (Cu2O) are aggregated on the outside surface of SBA-15, and the other Cu+ species are highly dispersed in SBA-15 via ≡Si-O-Cu. Its adsorption mechanism was firstly studied via comparison with CuO/SBA-15. The TC adsorption kinetic of Cu+/SBA-15 fitted the pseudo-second-order model well. The adsorption isotherms at 293, 303, and 313 K were determined and modeled with Langmuir and Freundlich equations, and the Qmax of Cu+/SBA-15 for TC calculated from Langmuir model could reach 961.54 mg/g at 313 K. Its good adsorption performance for TC is associated with the high surface area of SBA-15, highly dispersed Cu+ species, and mesoporous structure. The adsorption of Cu+/SBA-15 for TC is an endothermic process, and adsorption heat is 66.88 kJ·mol−1, which implies that the adsorption process of Cu+/SBA-15 for TC is weak chemisorption. The adsorption mechanism of Cu+/SBA-15 was mainly explored via comparing with CuO/SBA-15 by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) spectroscopy analysis. Besides the coordination complexation of Cu+ with NH2 radical of TC, the π-complexation between Cu+, and the benzene ring of TC improved the adsorption capacity of Cu+/SBA-15 for TC comparing with CuO/SBA-15 and showed a dominant role for TC adsorption.
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•p-Nitrobenzamide solubility in four co-solvent mixtures was determined and correlated.•Preferential solvation of p-nitrobenzamide in four mixtures were derived by IKBI ...method.•Solvent effect was studied based on solute–solvent and solvent–solvent interactions.
The equilibrium p-nitrobenzamide solubility in mixtures of dimethyl sulfoxide (DMSO, 1) + water (2), ethanol (1) + water (2), isopropanol (1) + water (2) and ethylene glycol (EG, 1) + water (2) was determined with a shake-flask method from (293.15 to 328.15) K under atmospheric pressure (101.1 kPa). At the same temperature and mass fraction of DMSO (ethanol, isopropanol or EG), the mole fraction solubility of p-nitrobenzamide was greater in (DMSO + water) than in ethanol (1) + water (2), isopropanol (1) + water (2) and ethylene glycol (EG, 1) + water (2) mixtures. X-Ray power diffraction analysis of solid phase showed no polymorphic transformation or solvate formation in experiments. The degree of relative importance of solute–solvent and solvent–solvent interactions on the solubility variation was evaluated by the linear solvation energy relationship analysis of solvent effect. The inverse Kirkwood–Buff integrals was used to study the preferential solvation based on thermodynamic solution properties. The parameters of preferential solvation for ethanol and isopropanol were positive in the ethanol (1) + water (2) and isopropanol (1) + water (2) mixtures in co-solvent-rich and intermediate composition, indicating that p-nitrobenzamide was solvated preferentially by the co-solvent. p-Nitrobenzamide acted mainly as a Lewis acid interacting with proton-acceptor functional groups of the co-solvents. Moreover, the drugs’ solubility was represented mathematically via the Jouyban-Acree model attaining the average relative deviations smaller than 2.16%.
Cardiac fibrosis, a common pathology in inherited and acquired heart diseases, necessitates the identification of diagnostic and therapeutic targets. Methyltransferase Like 1 (METTL1), an enzyme ...responsible for RNA modification by methylating guanosine to form m7G, is an emerging area of research in understanding cellular processes and disease pathogenesis. Dysregulation of m7G modification has been implicated in various diseases. However, the role of METTL1 in cardiac fibrosis remains unclear. This study aimed to investigate the role of METTL1 in myocardial infarction-induced heart failure and cardiac fibrosis. Our findings demonstrate that elevated METTL1-mediated RNA m7G methylation is observed in cardiac fibrosis tissues and TGF-β1-induced cardiac fibroblast proliferation and myofibroblast transformation. Furthermore, fibroblast-specific knockout of METTL1 attenuated myocardial infarction-induced heart failure and cardiac fibrosis. Additionally, METTL1 knockout decreased m7G methylated fibrotic genes and impaired their translation efficiency. These results suggest a novel pro-fibrosis role of METTL1-mediated RNA m7G methylation, highlighting its potential as a therapeutic target in cardiac fibrosis.
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Vascular smooth muscle cell (VSMC) proliferation and neointima formation play significant roles in atherosclerosis development and restenosis following percutaneous coronary intervention. Our team ...previously discovered that TEA domain transcription factor 1 (TEAD1) promotes vascular smooth muscle differentiation, which is necessary for vascular development. Conversely, aberrant YAP1 activation upregulates the platelet-derived growth factor receptor beta to encourage VSMC proliferation and neointima formation. In this study, we aimed to investigate the molecular mechanisms of YAP1/TEAD signaling during neointima formation. Our research focused on the prolyl 4-hydroxylase alpha 2 (P4HA2) and its downstream target, Yes-associated protein 1 (YAP1), in regulating VSMC differentiation and neointima formation. Our results indicated that P4HA2 reduction leads to VSMC dedifferentiation and promotes neointima formation after injury. Furthermore, we found that P4HA2-induced prolyl hydroxylation of YAP1 restricts its transcriptional activity, which is essential to maintaining VSMC differentiation. These findings suggest that targeting P4HA2-mediated prolyl hydroxylation of YAP1 may be a promising therapeutic approach to prevent injury-induced neointima formation in cardiovascular disease.
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Cu+-modified SBA-15 mesoporous adsorbents (Cu(I)/SBA-15) were prepared via a low temperature reduction with diluted hydrazine hydrate aqueous solution as reduction agent. The samples were ...characterized by XRD, N2 adsorption-desorption isotherms, TEM, XPS, and H2-TPR. It was revealed that the amount and state of Cu+ species on SBA-15 was significantly affected by the reduction temperature. The Cu+ ratio of the total amount of copper species increased when the reduction temperature increased from 20 °C to 40 °C, the Cu+ species have a high dispersion on the SBA-15, and the mesoporous structure of SBA-15 still keeps intact. In contrast, when the reduction temperature was raised up to 50 °C, the Cu+ amount obviously decreased, some aggregated Cu2O formed on SBA-15, and the SBA-15 matrix suffered partial damage. The thiophene adsorption capacity on the Cu(I)/SBA-15(n) was measured, and the Cu(I)/SBA-15(40) shows a higher adsorption capacity than the other samples due to the high Cu+ ratio of the total amount of copper species (80.77%) in Cu(I)/SBA-15(40). The adsorption equilibrium data were described by the Langmuir isotherm models and the maximum adsorption capacity (qm) of thiophene adsorbed by Cu(I)/SBA-15(n) was calculated by Langmuir model.
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•Cu+-modified SBA-15 was prepared by a facile low temperature reduction.•Higher Cu+ amount and well-dispersed Cu+ species on SBA-15 were achieved.•The Cu(I)/SBA-15 with higher Cu+ amount has higher adsorption capacity and better selectivity for thiophene.
This work investigated the feasibility and efficiency of a heterogeneous photo-Fenton catalyst, Fe/Si codoped TiO2, for the degradation of metronidazole (MNZ) under visible light irradiation. The ...Fe/Si codoped TiO2 was prepared via a facile and simple sol–gel solvothermal process followed by annealing at 480 °C for 4 hours. High resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS) measurements revealed that the photo-Fenton process did not change the structure, textural and surface morphologies of this catalyst. Elemental mapping results indicated the good dispersion of Fe and Si ions in TiO2. Nitrogen adsorption and desorption measurements indicated that Si doping increased the surface area of the catalysts. The Fe and Si doping narrowed the band gap of TiO2. They also facilitated the transfer of photo-generated electrons from TiO2 to Fe(iii). Under visible light irradiation and the optimum operating conditions, MNZ could be completely degraded in 50 min by this catalyst within a wide pH range. Hydroxyl radicals and holes were verified to be responsible for degrading MNZ. The leaching of iron ions was less than 0.047 ppm even after illuminating the catalyst for 6 hours, indicating the good stability of the Fe/Si codoped TiO2. The as-prepared catalysts with excellent catalytic activity, and remarkable reusability and stability could provide a new insight into the preparation of photocatalysts and have wide applications for antibiotics removal.