Genetic diversity and phylogenetic relationship of three Croatian autochthonous cattle breeds was analyzed using a sequence of the mtDNA D-loop region. Among Busha, Istrian, and Slavonian Syrmian ...Podolian cattle 146 unrelated animals were tested. The sequencing of 780 base pairs of the mtDNA D-loop region revealed 39 polymorphic sites representing 28 different haplotypes. The highest numbers of haplotypes were observed in the Busha population and the lowest in the population of Slavonian Syrmian Podolian cattle, while the highest level of sequence diversity within a population was observed in the Istrian cattle. Our results indicate a high level of mtDNA diversity in the populations of Busha and Istrian cattle and a low level of preserved diversity in the population of Slavonian Syrmian Podolian cattle. The sequence analysis showed substantial subdivision between the breeds (FST = 0.1434), and a large fraction of variation within the breeds. Although the dominant haplotypes are classified as the T3 haplogroup, some of the haplotypes are classified as the rarer T2 and T5 haplogroups. Genetic information based on mtDNA typing has a great importance for the future conservation management and preservation of genetic diversity in autochthonous cattle breeds.
The objectives of this study were to determine allele frequency and genotype count of thyroglobulin (TG) and diacylglycerol-0-acyltransferase-1 (DGAT1) genes encoding the TG and DGAT1 enzymes, and to ...determine the effect of TG and DGAT1 polymorphisms on fatty acid profile in beef carcass. All genotypes were determined by PC-RFLP assay. Polymorphism of TG and DGAT1 had no significant influence on total lipid content, backfat thickness, EUROP and carcass conformation score. The TT genotype of TG gene resulted in a lower total lipid content and backfat thickness, while KK genotype of DGAT1 gene showed a greater total lipid content and backfat thickness. The content of the majority fatty acids in MLD and subcutaneous fat tissue was not significantly affected by TG and DGAT1 polymorphism. The results confirm the lack of an association found by other studies. Further research should be carried out to validate the initial observation.
The aim of this research was to determine differences in beef carcass fatness and in the fatty acid composition of muscle and adipose tissue of three different breeds slaughtered at the age of 14 ...months and 19 months. The breed significantly affected the muscle fat content, carcass subcutaneous fat tissue thickness and fatty acid composition of the muscle and subcutaneous fat tissue. Different age at slaughter had no significant effect on analysed traits. The muscle tissue of the Herefords contained a higher (P < 0.05) percentage of C14 : 0 and C16 : 0 fatty acids and fewer long-chain fatty acids than the Simmentals and Charolais. The subcutaneous fat tissue of the Simmentals contained a higher (P < 0.05) percentage of PUFA, PUFA/SFA ratio and n-6 fatty acids. When fed with a high-energy diet, the Herefords proved to have the most fattened carcasses and the highest content of saturated fatty acids in the muscle. Changes in the diet of feedlot cattle should result in a more favourable fatty acid composition.
Mitochondrial D-loop sequence variation among autochthonous horse breeds in Croatia Ivankovic, A.,University of Zagreb (Croatia). Dept. of Animal Production and Technology; Ramljak, J.,University of Zagreb (Croatia). Dept. of Animal Production and Technology; Konjacic, M.,University of Zagreb (Croatia). Dept. of Animal Production and Technology ...
Czech Journal of Animal Science,
03/2009, Letnik:
54, Številka:
3
Journal Article
Recenzirano
Odprti dostop
Genetic variation in three Croatian cold-blood horse populations was analysed using a sequence analysis of the proximal part (nt 15 498-15 821) of the D-loop region of mtDNA. Twenty unrelated horses ...were chosen from the Posavina horse and the Croatian cold-blood breeds and fifteen horses from the Murinsulaner horse population. Sequencing of the proximal part of the mtDNA D-loop region revealed 26 polymorphic sites representing thirty haplotypes which were clustered into eight haplogroups. A wide variety of mitochondrial haplotypes of the analysed horse breeds were clustered into eight different haplogroups. Two haplogroups (D and F) were specific to Posavina horse, five haplogroups were shared among the Croatian cold-blood and Murinsulaner horse, the fact that can be explained by selection strategy at the beginning of the 20th century and possible gene flow between the two populations. These results indicate the presence of many ancient maternal lineages with high diversity in mtDNA. The genetic information based on mtDNA typing has a great importance for the future breed conservation strategy, especially for the critically endangered breed such as Murinsulaner horse.
Microsatellite analysis of the genetic diversity in the Black Slavonian pig Bradic, M., University of Zagreb, Zagreb (Croatia). Faculty of Agriculture, Department of Animal Science; Uremovic, M., Faculty of Agriculture, Zagreb (Croatia); Uremovic, Z., Faculty of Agriculture, Zagreb (Croatia) ...
Acta veterinaria (Beograd),
(2007), 2007-00-00, Letnik:
57, Številka:
2-3
Journal Article
Recenzirano
Odprti dostop
The research is focused on the genetic diversity and population structure of the Black Slavonian pig, with the aim of assessing the situation regarding the endangerment of genetic diversity, and ...defining the further role of this breed in the preseration of its genetic diversity. Genetic population parameters were assessed in 42 animals at 8 microsatellite loci.
Dispersion parameters for the number of piglets born alive were estimated using a repeatability and random regression model. Six sow breeds/lines were included in the analysis: Swedish Landrace, ...Large White and both crossbred lines between them, German Landrace and their cross with Large White. Fixed part of the model included sow genotype, mating season as month-year interaction, parity and weaning to conception interval as class effects. The age at farrowing was modelled as a quadratic regression nested within parity. The previous lactation length was fitted as a linear regression. Random regressions for parity on Legendre polynomials were included for direct additive genetic, permanent environmental, and common litter environmental effects. Orthogonal Legendre polynomials from the linear to the cubic power were fitted. In the repeatability model estimate of heritability was 0.07, permanent environmental effect as ratio was 0.04, and common litter environmental effect as ratio was 0.01. Estimates of genetic parameters with the random regression model were generally higher than in the repeatability model, except for the common litter environmental effect. Estimates of heritability ranged from 0.06 to 0.10. Permanent environmental effect as a ratio increased along a trajectory from 0.03 to 0.11. Magnitudes of common litter effect were small (around 0.01). The eigenvalues of covariance functions showed that between 7 and 8 % of genetic variability was explained by individual genetic curves of sows. This proportion was mainly covered by linear and quadratic coefficients. Results suggest that the random regression model could be used for genetic analysis of litter size.
Istarsko govedo najbrojnija je autohtona pasmina goveda u Hrvatskoj. Predstavlja živi spomenik vremena i podneblja o kojem gotovo dva desetljeća brinu vrijedni uzgajivači i šira društvena zajednica. ...Zaustavljena su negativna kretanja u populaciji istarskog goveda izazvana globalnim trendovima u animalnoj proizvodnji gospodarski razvijenijih društava, a aktualna populacija nalazi se u fazi oporavka i blage stagnacije. Usporavanje daljnjeg rasta populacije uvjetovano je izostankom provjerene gospodarske reafirmacije koja treba interesno povezati uzgajivače i potrošače, osiguravajući time samoodrživost pasmine. Pokrenuti projekt reafirmacije istarskog goveda kroz program proizvodnje ''Mesa istarskog goveda'' potakao je konsenzus svih subjekata u slijedu namirnice (uzgoj, procesing, gastronomija), te daje prve pozitivne učinke.
Hrvatske autohtone pasmine goveda dio su skupine ugroženih europskih pasmina domaćih životinja. Genetska različitost je esencijalna za očuvanje adaptivnog potencijala pasmina i unapređenja njihovog ...proizvodnog potencijala u bližoj i daljnjoj budućnosti. Nadzor genetske različitosti je temelj učinkovitih konzervacijskih programa. Analiza rodoslovlja je korisna u upravljanju konzervacijskom strategijom. U iznesenom radu istražili smo odnose rodoslovnih informacija autohtonih pasmina goveda u cilju sjecanja uvida u populacijsku strukturu i način upravljanja genetskom varijabilnošću. Analizom je obuhvaćeno 3017 rodoslovnih zapisa istarskog goveda, 578 rodoslovnih zapisa slavonsko-srijemskog podolca i 898 rodoslovnih zapisa buše, registriranih u središnjoj matičnoj knjizi u razdoblju od 1994. do 2009. Pozitivni populacijski trendovi u svim autohtonim pasminama uočljivi su kroz povećanje broja uzgojno valjanih jedinki i teladi godišnje registriranih u središnji matični registar, uz uočljivu stagnaciju u razdoblju od 2002. do 2004. Viši koeficijent uzgoja u srodstvu i vrijednost koeficijenta prosječne srodnosti jedinki u populaciji istarskog goveda ukazuju na potrebu redizajna konzervacijske strategije. Glavni razlog tomu je korištenje bikova uglavnom rođenih u istom ili srodnom stadu, odnosno nedovoljna razmjena životinja između stada. U populaciji slavonsko-srijemskog podoloca uočeno je 'usko grlo', te se ukazuje potreba usklađivanja sheme sparivanja uzgojno valjanih jedinki. Udio visoko srodnih jedinki u populacijama istarskog goveda i slavonsko-srijemskog podoloca je značajan. Najmanja razina uzgoja u srodstvu i najmanji koeficijent prosječne srodnosti jedinki u populaciji zapaženi su u populaciji buše. Uočene informacije mogu biti vrlo korisne u provedbi konzervacijskih programa očuvanja hrvatskih autohtonih ugroženih pasmina goveda.
PDF
