In this volume university faculty members describe their collaborative projects with other stakeholders in the educational enterprise. Through descriptions of several collaborative projects, the ...chapters explore some of the less explicitly articulated aspects of collaborative ventures.
A genetic strategy identified Salmonella typhimurium strains carrying large (> 40 kb) Tn 10-catalyzed inversions; the inverted segments were characterized by XbaI digestion and pulsed field gel ...electrophoresis. Two size classes of large inversions were found. More than half of the inversions extended 40-80 kb either clockwise or counterclockwise of the original Tn10 site. The remaining inversions extended up to 1620 kb (33% of the genome), but the distal endpoints of these inversions were not randomly scattered throughout the chromosome. Rather, each Tn10 repeatedly yielded similar (though not identical) inversions. The biased endpoint selection may reflect the limited search for target DNA sequences by the Tn10 transposase, and the spatial proximity of the donor and target regions in the folded S. typhimurium nucleoid. Using this interpretation, the data suggest that DNA sequences 40-80 kb clockwise and counterclockwise of the insertion site are in spatial proximity with the insertion, perhaps reflecting the organization of DNA into approximately 120-kb nucleoid domains. In addition, the data predict the spatial proximity of several distant DNA regions, including DNA sequences equidistant from the origin of DNA replication.
An enhanced appreciation of internalization of antisense oligonucleotides will extend possible applications in experimental and therapeutic settings. We found that oligonucleotide incorporation in ...monocytes, granulocytes and B-lymphocytes but not in T-lymphocytes is strongly dependent on the presence of extracellular calcium, and is inhibited by heparin. Our results support the hypothesis that calcium-dependent adhesion molecules mediate oligonucleotide internalization in leukocytes.
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