We report on the role of hexamethylene-bis-acetamide-inducible protein 1 (HEXIM1) as an inhibitor of metastasis. HEXIM1 expression is decreased in human metastatic breast cancers when compared with ...matched primary breast tumors. Similarly we observed decreased expression of HEXIM1 in lung metastasis when compared with primary mammary tumors in a mouse model of metastatic breast cancer, the polyoma middle T antigen (PyMT) transgenic mouse. Re-expression of HEXIM1 (through transgene expression or localized delivery of a small molecule inducer of HEXIM1 expression, hexamethylene-bis-acetamide) in PyMT mice resulted in inhibition of metastasis to the lung. Our present studies indicate that HEXIM1 downregulation of HIF(-)1α protein allows not only for inhibition of vascular endothelial growth factor-regulated angiogenesis, but also for inhibition of compensatory pro-angiogenic pathways and recruitment of bone marrow-derived cells (BMDCs). Another novel finding is that HEXIM1 inhibits cell migration and invasion that can be partly attributed to decreased membrane localization of the 67 kDa laminin receptor, 67LR, and inhibition of the functional interaction of 67LR with laminin. Thus, HEXIM1 re-expression in breast cancer has therapeutic advantages by simultaneously targeting more than one pathway involved in angiogenesis and metastasis. Our results also support the potential for HEXIM1 to indirectly act on multiple cell types to suppress metastatic cancer.
The article presents the results of clinical studies of domestic cats and dogs with diabetes mellitus on the background of obesity at the beginning and for a month of treatment. Domestic cats with ...diabetes were prescribed active exercise and industrial diet Royal Canin Diabetic Feline, dogs – Royal Canin Diabetic DS37. The portion size was determined according to the manufacturer's recommendations. Treatment of small animals for obesity, both alimentary and endocrine, preferably involves feeding food in limited quantities to cause controlled weight loss, as well as increased physical activity, which can provide additional energy expenditure. Successful weight loss can reduce the effects of comorbidities associated with obesity, such as improved mobility in osteoarthritis, increased insulin sensitivity and the reversal of other metabolic disorders, and improved quality of life. The main pathogenetic therapy for animals with diabetes for both domestic cats and dogs is the mandatory use of insulin. Researching the market of drugs in Ukraine, we chose the most affordable and effective veterinary drug – Caninsulin. The dose of insulin was selected individually for each animal by plotting a curve of blood glucose concentration over three days. At the beginning of treatment and at the end of the course, we conducted a comprehensive study of animals of both species. In cats, the general condition significantly improved as a result of treatment: only one animal remained depressed, the number of cases of hyporexia decreased, only 25.0 % of animals remained pale mucous membranes; temperature within the physiological norm; vomiting and bradycardia were not reported. A decrease in the rate of shortness of breath and drowsiness also indicates an improvement in the condition of cats with obesity due to diabetes. The improvement of the general condition of domestic dogs is evidenced by the restoration of the condition of the coat (about 4 times), the restoration of appetite in 66.7 %. Only one dog had anemia of the mucous membranes. Anxiety, bradycardia, hypothermia and itching disappeared completely. Within one month, there was a tendency to decrease the body weight of animals of both groups, but due to the insufficient duration of treatment, their fatness still remained excessive. Which indicates the need for further correction of the pathological condition.
Clinical signs of hypothyroidism in domestic dogs Lokes-Krupka, T. P.; Tsvilichovsky, M. I.; Zarytskyi, S. M.
Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки,
10/2020, Letnik:
22, Številka:
99
Journal Article
Recenzirano
Odprti dostop
The research was conducted during 2016–2019 on the basis of veterinary clinics in Poltava - veterinary clinic at the Department of Therapy named after Professor P.Lokes of Poltava State Agrarian ...Academy, “Vet Line”, “Vet Comfort” and the laboratory of the Department of Therapy named after Professor P. I. Lokes of Poltava State Agrarian Academy. During the research we studied 11 dogs, which were diagnosed with hypothyroidism. The animals belonged to different breeds, had different sexes (6 females and 5 males) and age. For control we took 15 dogs without visible signs of any pathology. We compared the breed susceptibility of dogs with hypothyroidism and dogs with alimentary obesity. Thus, we found that most breeds are common to animals in both pathologies. However, in our sample for hypothyroidism there was no such breed as a pug, and outbred individuals, which accounted for a significant percentage of dogs with alimentary obesity. One of the most common clinical symptom is the presence of symmetrical alopecia on the sides on the background of dull and disheveled hair (90.9 %). Mucosal anemia was observed in a significant proportion of animals (81.8 %). The symptom of bradycardia and shortness of breath during exercise is observed in 72.7 % of experimental animals, which indicates the development of pathological changes in the cardiovascular system. It was found that all animals showed depression, weakness and fatigue (100.0 %). Polydipsia and hypothermia were much less common in 45.5 % of dogs, and corneal dystrophy in 36.4% of cases. The last clinical manifestation occurred in animals with the most severe pathology. According to the literature, it is known that hypothyroidism in dogs is often a symptom of obesity. According to our data, obesity (overweight) was registered in 63.6 % of experimental animals. With the diagnosis of “hypothyroidism” in dogs, we identified two experimental groups: 1 – animals with normal fatness; 2nd – with a clinical symptom of obesity. We can note a significant increase in adipose tissue in dogs with hypothyroidism of the 2nd experimental group (45.1 ± 1.70 %), which indicates the presence of endocrine obesity, as evidenced by visual assessment of fatness (4.7 ± 0.18 points). Such changes develop due to insufficient production of thyroid hormones. The latter play an important role in regulating metabolism in the body. Due to their deficiency, the metabolism in animals slows down and, as a result, there is a risk of obesity.
Clinical case of chronic hepatitis in a domestic dog Lokes-Krupka, T. P.; Vlokh, I. Yu; Baklytska, A. S. ...
Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки,
10/2022, Letnik:
24, Številka:
107
Journal Article
Recenzirano
Odprti dostop
The article presents a clinical case of chronic hepatitis of alimentary origin in a domestic dog of the Labrador Retriever breed, aged 1.5 years. General depression, hyporexia, intermittent morning ...vomiting, and diarrhea have been reported clinically. According to the anamnesis data, violations of the animal's lifelong feeding regime were established, as well as non-compliance with the rules of preventive deworming and dosing of drugs. The study in a sick dog showed a slight increase in body temperature (39.5 °C); heart rate and respiration were within normal limits. Palpation did not detect liver tenderness, but the animal's anxiety was recorded when palpating the abdomen. The obtained results were confirmed by ultrasonography. Thus, well-defined contours (edges) of the liver were visualized. The obtained results were confirmed by ultrasonographic examination. Thus, it visualized the enlargement of the liver and rounding of the edges of the body. The echostructure of the body is uneven. The gallbladder was round, with a thickening of the walls, and contained biliary sludge. Increased visualization of hepatic vessels was characteristic. Functional changes in liver function have been established. In a sick dog's serum, the total protein content was 78.3 g/l. A study of total bilirubin revealed a significant increase (9.5 μmol/l). Transaminase activity increased almost 1.7 times and corresponded to 97.0 IU/l (ALT) and 76.3 IU/l (AST). GHTP and alkaline phosphatase activity also underwent upward changes (8,7 and 8,3 IU/l, respectively). The urine had a rich orange color; at the same time, it was cloudy, the consistency was watery and foamy, and the smell was nonspecific/unpleasant. Determination of chemical properties of urine showed: the presence of proteins, urobilinogen; bilirubin; while glucose and ketones - were negative. Single leukocytes were detected by sediment microscopy. Thus, considering the data obtained from a comprehensive study of a sick animal, it can be noted that the final diagnosis in Labrador Retriever dogs is chronic hepatitis. To correct the pathological condition, a scheme of pharmacotherapy was drawn up, which consisted of drugs to restore liver cells' functioning, reduce body intoxication, and improve metabolism. At the same time, a diet (Royal Canin Hepatic Dog) was introduced, with mandatory adherence to the feeding regime.
The genus Heterakis Dujardin, 1845 is one of the most widely distributed genera of nematode parasites of domestic and wild birds. Geographically and ecologically predominant species include Heterakis ...gallinarum Schrank, 1788 and Heterakis dispar Schrank, 1790. Prevalence and occurrence of these nematodes in the domestic goose (Anser anser dom.) depend on their biological and morphological specifics which ensure the highest viability, fast growth and maximum fertility in the host. This study presents the abundance and species composition of nematodes of the genus Heterakis in the domestic goose in Ukraine. Comparative study of morphological and metrical characteristics of H. gallinarum and H. dispar adult females and males is presented. Additional morphometric characteristics are proposed for easier identification of the two species. Stages and periods of embryonic development of H. gallinarum and H. dispar nematodes obtained from domestic geese are established according to morphological and metrical characters. Of the two species, H. dispar is prevalent in domestic geese from Poltava, Kharkiv and Kyiv regions. Abundance index of this species is 9.8 specimens, and intensity of infection index is up to 62 specimens. H. gallinarum is rarer, its abundance index is 1.2 specimens and maximum intensity of infection is 30 specimens. Species-specific morphological differences are more distinct in male nematodes in the size and structure of the spicules, lateral wing-like protrusions of pseudobursas, numbers and position of tail papillae. Additional metrical characteristics differ between H. gallinarum and H. dispar nematodes, allowing us to identify not only adult specimens but also eggs. Embryonic development of both species occurs in four morphologically distinct stages: protoplast, blastomere cleavage, formation of first and second stage larvae. Eggs of H. gallinarum nematodes become infectious in eight days at 27 °С, eggs of H. dispar in four days, their viability in laboratory culture is 84.3 ± 0.58 and 91.3 ± 1.53% respectively.
The objective of this study was to examine the effects of three Pluronic triblock copolymers (F127, P85, or L61) on the cytotoxicity of carboplatin to the DHB/K12/TRb rat colorectal carcinoma cell ...line. Studies to determine the dependence of the sensitization effect on Pluronic dose were carried out for polymer concentrations ranging from 0.0001–10% (w/w). To establish the carboplatin toxicity and its potential enhancement by Pluronic, the drug was delivered to cells in doses ranging from 0–0.5% (w/w) in the presence of Pluronic at a constant concentration. These treatment groups were compared to control groups receiving carboplatin alone. Cell cytotoxicity resulting from the treatments was determined with a mitochondrial enzyme activity assay (WST-1), while cell morphology was examined with May–Grünwald and Giemsa staining. Results indicate that the greatest enhancement of carboplatin toxicity was induced by P85, where the inhibitory concentration (IC
50) was reduced by 50% (from 0.096 mg/mL for carboplatin alone to 0.048 mg/mL in presence of P85). L61 was toxic to the cells with or without drug (viability
<
3.5%), while F127 exhibited no sensitizing effect and in some cases increased the cell viability to 130% over the untreated control. The WST-1 results were confirmed by trypan blue exclusion cell counts at 0 and 24 h post treatment. Data conclusively demonstrate that Pluronic P85 is the optimal agent for increased cytotoxicity of carboplatin in this cell line and can potentially be used not only as a drug delivery scheme but also as a chemosensitizing agent in future cancer therapy.
Aim: Many scientific reports indicate that optimism positively affects our psychophysical well-being. The main purpose of the conducted research was to verify whether optimism is a predictor of ...health-oriented resources also in patients with paranoid schizophrenia, depressive disorders and depressive schizoaffective disorders. The following health-oriented factors were evaluated in the study: health behaviour, life satisfaction, social functioning and coping with stress. Furthermore, we have verified whether there were any differences related to the level of optimism and health-oriented factors between individuals with mental disorders and a control group. Methods: The following tools were used in the study: Optimism Questionnaire, Health-Related Behaviour Inventory, Coping Inventory for Stressful Situations, Satisfaction with Life Scale, Social and Occupational Functioning Assessment Scale, Beck Depression Inventory. Results: Effects of optimism on some of the health-oriented factors were observed in patients with mental disorders as well as in healthy individuals. Significant differences in the level of health-oriented resources between the study population and controls were reported. Conclusions: The study suggests that generalisation of the conclusions regarding healthy population to individuals with mental disorders should be done with caution. Further research is needed to investigate the health behaviour determinants in patients with mental disorders.
This volume contains invited lectures and selected research papers in the fields of classical and modern differential geometry, global analysis, and geometric methods in physics, presented at the ...10th International Conference on Differential Geometry and its Applications (DGA2007), held in Olomouc, Czech Republic.The book covers recent developments and the latest results in the following fields: Riemannian geometry, connections, jets, differential invariants, the calculus of variations on manifolds, differential equations, Finsler structures, and geometric methods in physics. It is also a celebration of the 300th anniversary of the birth of one of the greatest mathematicians, Leonhard Euler, and includes the Euler lecture "Leonhard Euler - 300 years on" by R Wilson. Notable contributors include J F Cariñena, M Castrillón López, J Erichhorn, J-H Eschenburg, I Kolář, A P Kopylov, J Korbaš, O Kowalski, B Kruglikov, D Krupka, O Krupková, R Léandre, Haizhong Li, S Maeda, M A Malakhaltsev, O I Mokhov, J Muñoz Masqué, S Preston, V Rovenski, D J Saunders, M Sekizawa, J Slovák, J Szilasi, L Tamássy, P Walczak, and others.Sample Chapter(s)Chapter 1: Leonhard Euler - 300 Years On (351 KB)Contents: Riemannian GeometrySubmanifoldsGeometric StructuresGlobal AnalysisGeometric Methods in PhysicsReadership: Researchers in differential geometry and mathematical physics.
PDF
