Phylogenomics of Life Kingdom's Deubiquitinases Evpak, Alena; Evseev, Peter; Kudriaeva, Anna ...
2023 IEEE Ural-Siberian Conference on Computational Technologies in Cognitive Science, Genomics and Biomedicine (CSGB),
2023-Sept.-28
Conference Proceeding
Eukaryotic organisms typically have many types of deubiquitinases, which differ in substrate specificity and the type of linkage of polyubiquitin chains 1. Despite the high degree of structural ...variability of deubiquitinases, the catalytic domains of these enzymes are quite conservative. For example, all eukaryotic cysteine proteases have a characteristic papain-like fold 2. In contrast to eukaryotes, the diversity of bacterial deubiquitinating enzymes is significantly reduced: globally, majority of bacterial deubiquitinases belong to either the OTU family or the CE-clan, which are also papain-like enzymes. 3. Another difference between bacterial deubiquitinases is their narrower substrate specificity. Archaeal deubiquitinases belong to the JAMM family of metalloproteases and are similar to the eukaryotic deubiquitinase RPN11 4. Despite the obvious differences in the diversity and representation of various types of deubiquitinating enzymes in different domains of living organisms, one can trace common features in the structure of individual classes of deubiquitinases from evolutionarily distant groups of organisms. Here we report phylogenetic analysis of this group of proteins in different representatives of the three main domains of living organisms. The results, obtained in this research can suggest the events of horizontal exchanges between the members of Life Kingdoms.
