Diets containing high proportions of fruits and vegetables reduce the risk of onset of chronic diseases. The role of herbal medicines in improving human health is gaining popularity over the years, ...which also increases the need for safety and efficiency of these products. Green leafy vegetables (GLVs) are the richest source of phenolic compounds with excellent antioxidant properties. Increased consumption of diets containing phenolic compounds may give positive and better results to human health and significantly improves the immune system. Highly selective, susceptible and versatile analytical techniques are necessary for extraction, identification, and quantification of phenolic compounds from plant extracts, which helps to utilize their important biological properties. Recent advances in the pre-treatment procedures, separation techniques and spectrometry methods are used for qualitative and quantitative analysis of phenolic compounds. The online coupling of liquid chromatography with mass spectrometry (LC–MS) has become a useful tool in the metabolic profiling of plant samples. In this review, the separation and identification of phenolic acids and flavonoids from GLVs by LC–MS have been discussed along with the general extraction procedures and other sources of mass spectrometer used. The review is devoted to the understanding of the structural configuration, nature and accumulation pattern of phenolic acids and flavonoids in plants and to highlighting the recent developments in the chemical investigation of these compounds by chromatographic and spectroscopic techniques. It concludes with the advantages of the combination of these two methods and prospects.
Though microalgae and macroalgae are considered as a potential feedstock for biofuel and industrially important co-products extraction, still there are several research barriers on the ...commercialization of algae-based fuels and products. Based on these bottlenecks, this review underpins the biochemical composition of micro- and macroalgae regarding biofuel production and bioactive compounds extraction. Further, the second chapter summarizes the various cultivation systems for rapid generation of macroalgal biomass. Micro- and macroalgae are untapped for bioenergy production to assess the feasibility of future green fuel sustainability. In general, algae were considered as a potential source for various applications worldwide owing to their rich and enormous bioactive potential. Therefore, a separate section devoted to recognize the crucial role and biological activities of primary and secondary metabolites in micro- and macroalgal species, their significant contribution as functional foods or therapeutic agents in nutraceutical and pharmaceutical industries. The extensive discussion on the phenolics, flavonoids and pharmacological properties of other bioactive compounds extracted from microalgae has been provided. Further, carbohydrates, proteins (phycobiliproteins and phycoerythrin) and their organic extraction from macroalgal strains (seaweeds) were well described. This review paper describes the importance of bioactive compounds and their value in the various other markets besides biofuel production.
Display omitted
•Insights on biochemical composition of micro- and macroalgae were given.•Flaws in bioenergy production from microalgae and macroalgae were addressed.•Bioactive compounds from microalgae and their applications were presented.•Algal co-products in pharmacology and nutraceutical were critically discussed.•High-value products of macroalgae and their extraction were reviewed and described.
Over 380 host plant species have been known to develop leaf spots as a result of the fungus Alternaria alternata. It is an aspiring pathogen that affects a variety of hosts and causes rots, blights, ...and leaf spots on different plant sections. In this investigation, the lipopeptides from the B. subtilis strains T3, T4, T5, and T6 were evaluated for their antifungal activities. In the genomic DNA, iturin, surfactin, and fengycin genes were found recovered from B. subtilis bacterium by PCR amplification. From different B. subtilis strains, antifungal Lipopeptides were extracted, identified by HPLC, and quantified with values for T3 (24 g/ml), T4 (32 g/ml), T5 (28 g/ml), and T6 (18 g/ml). To test the antifungal activity, the isolated lipopeptides from the B. subtilis T3, T4, T5, and T6 strains were applied to Alternaria alternata at a concentration of 10 g/ml. Lipopeptides were found to suppress Alternaria alternata at rates of T3 (75.14%), T4 (75.93%), T5 (80.40%), and T6 (85.88%). The T6 strain outperformed the other three by having the highest antifungal activity against Alternaria alternata (85.88%).
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The direct release of industrial effluent into the water and other anthropogenic activities causes water pollution. Heavy metal ions are the primary contaminant in the industrial effluents which are ...exceptionally toxic at low concentrations, terribly disturb the endurance equilibrium of activities in the eco-system and be remarkably hazardous to human health. Different conventional treatment methodologies were utilized for the removal of toxic pollutants from the contaminated water which has several drawbacks such as cost-ineffective and lower efficiency. Recently, genetically modified micro-organisms (GMMs) stand-out for the removal of toxic heavy metals are viewed as an economically plausible and environmentally safe technique. GMMs are microorganisms whose genetic material has been changed utilizing genetic engineering techniques that exhibit enhanced removal efficiency in comparison with the other treatment methodologies. The present review comments the GMMs such as bacteria, algae and fungi and their potential for the removal of toxic heavy metals. This review provides current aspects of different advanced molecular tools which have been used to manipulate micro-organisms through genetic expression for the breakdown of metal compounds in polluted areas. The strategies, major limitations and challenges for genetic engineering of micro-organisms have been reviewed. The current review investigates the approaches working on utilizing genetically modified micro-organisms and effective removal techniques.
Display omitted
•Novel methodology on the removal of heavy metals were detailed.•GMOs are higher effective and considered as promising technique for heavy metal removal.•Extensive improvement of genetically modified micro-organisms by advanced molecular tools was summarized.•Genetic modification permits revamping microbial scavengers for the removal of hazardous pollutants.•Major limitations and challenges in genetic modification have been summarized for large-scale applications.
The present analysis reports the clinical, pathological, treatment profile and overall survival (OS) and disease-free survival (DFS) outcomes of consecutive breast cancer patients from three Indian ...centres, who underwent curative surgery as their first treatment. Among the 3453 patients, stage I, II, and III cases were 11.75%, 66.79%, and 21.64%, respectively while hormone receptor positive/HER2 negative, triple negative (TNBC) and hormone receptor any/HER2 positive cases were 55.2%, 24.2% and 20.6%, respectively. The five-year OS in the entire cohort, node-negative and node-positive patients were 94.1% (93.25-94.98), 96.17% (95.2-97.15) and 91.83% (90.36-93.31), respectively, and the corresponding DFS were 88.1% (86.96-89.31), 92.0% (90.64-93.39) and 83.93% (82.03-85.89), respectively. The five-year OS in hormone receptor positive/HER2 negative, TNBC and HER2 subgroups were 96.11% (95.12-97.1), 92.74% (90.73-94.8) and 90.62% (88.17-93.15), respectively, and the corresponding DFS were 91.59% (90.19-93.02), 85.46% (82.79-88.22) and 81.29% (78.11-84.61), respectively. This is the largest dataset of early breast cancer patients from India with survival outcome analysis and can therefore serve as a benchmark for future studies.
Heavy metals, dyes and pharmaceutical pollutants in water environment are considered as serious threat to the human and animal health globally. Rapid development of industrialization and agricultural ...activities are the major source for eliminating the toxic pollutants into the aquatic environment. Several conventional treatment methods have been suggested for the removal of emerging contaminants from wastewater. Algal biosorption, among other strategies and techniques, is demonstrating to be a limited technical remedy that is more focused and inherently more efficient and helps remove dangerous contaminants from water sources. The different environmental effects of harmful contaminants, including heavy metals, dyes, and pharmaceutical chemicals, as well as their sources, were briefly compiled in the current review. This paper provides a comprehensive definition of the future possibilities in heavy compound decomposition by using algal technology, from aggregation to numerous biosorption procedures. Functionalized materials produced from algal sources were clearly proposed. This review further highlights the limiting factors of algal biosorption to eliminate the hazardous material. Finally, this study showed how the existence of algae indicates a potential, effective, affordable, and sustainable sorbent biomaterial for minimizing environmental pollution.
Display omitted
•Algal biosorption is the economically viable and low-energy technological solution.•Biosorption mechanisms of algal biomass towards hazardous pollutants are discussed.•Functionalized materials derived from algal technology are reviewed.•Limiting factors and reactors involved in algal biosorption were elucidated.•Prospects and recommendations for future works were summarized.
Inadequate quality of care in healthcare facilities is one of the primary causes of patient mortality in low- and middle-income countries, and understanding the behavior of healthcare providers is ...key to addressing it. Much of the existing research concentrates on improving resource-focused issues, such as staffing or training, but these interventions do not fully close the gaps in quality of care. By contrast, there is a lack of knowledge regarding the full contextual and internal drivers-such as social norms, beliefs, and emotions-that influence the clinical behaviors of healthcare providers. We aimed to provide two conceptual frameworks to identify such drivers, and investigate them in a facility setting where inadequate quality of care is pronounced. Using immersion interviews and a novel decision-making game incorporating concepts from behavioral science, we systematically and qualitatively identified an extensive set of contextual and internal behavioral drivers in staff nurses working in reproductive, maternal, newborn, and child health (RMNCH) in government public health facilities in Uttar Pradesh, India. We found that the nurses operate in an environment of stress, blame, and lack of control, which appears to influence their perception of their role as often significantly different from the RMNCH program's perspective. That context influences their perceptions of risk for themselves and for their patients, as well as self-efficacy beliefs, which could lead to avoidance of responsibility, or incorrect care. A limitation of the study is its use of only qualitative methods, which provide depth, rather than prevalence estimates of findings. This exploratory study identified previously under-researched contextual and internal drivers influencing the care-related behavior of staff nurses in public facilities in Uttar Pradesh. We recommend four types of interventions to close the gap between actual and target behaviors: structural improvements, systemic changes, community-level shifts, and interventions within healthcare facilities.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Rapid industrialization and urbanization growth combined with increased population has aggravated the issue of municipal solid waste generation. MSW has been accounted for contributing tremendously ...to the improvement of sustainable sources and safe environment.
Biological processing of MSW followed by biogas and biomethane generation is one of the innumerable sustainable energy source choices. In the treatment of MSW, biological treatment has some attractive benefits such as reduced volume in the waste material, adjustment of the waste, economic aspects, obliteration of microorganisms in the waste material, and creation of biogas for energy use. In the anaerobic process the utilizable product is energy recovery. The current review discusses about the system for approaching conversion of MSW to energy and waste derived circular bioeconomy to address the zero waste society and sustainable development goals. Biological treatment process adopted with aerobic and anaerobic processes. In the aerobic process the utilizable product is compost. These techniques are used to convert MSW into a reasonable hotspot for resource and energy recovery that produces biogas, biofuel and bioelectricity and different results in without risk and harmless to the ecosystem. This review examines the suitability of biological treatment technologies for energy production, giving modern data about it. It likewise covers difficulties and points of view in this field of exploration.
•Review Highlights.•Effect of municipal solid waste on human and the environment is inspected.•Factors limiting the aerobic composting process have been comprehensively discussed.•Biological treatments for energy recovery from MSW were discussed.•Roadmap for circular economy using waste to energy techniques is proposed.
Background: The COVID-19 pandemic has modified the way, plastic surgeons treat their patients. This article depicts how we as a plastic surgery department in a tertiary care setup handled the ...pandemic with an emphasis on infection control policy. Methods: Data was collected from hospital records and quality assurance cell from March 21, 2020 to June 19, 2020 in terms of patient triaging, consultations, perioperative protocols, duty rosters, and academic activities. The changes on these with the impact of COVID-19 were studied with the same period of previous year. Results: Outpatient clinics were closed and emergency consultations were reduced. Number of consultations reduced from 2591 to 75 and surgeries from 320 to 46 during the same period in 2019 and 2020 respectively. Though tele-consultations were helpful, the overall benefits were subpar. Emergency services continued with the guidelines of institute's infection control committee, such as area specific personal protective equipment, allotment of operating rooms, minimizing the crowd in operating room. There was some compromise in using accessories for microsurgical procedures. Duty rosters were designed to maintain uninterrupted services. Academic activities were continued with virtual platforms. Conclusion: Adequate preparation of health care setup and nation-wide lockdown has helped to handle emergency cases and in reduction of trauma-surgeries respectively. Though there were obstacles for some patients in accessing health care, our institutional response made us to render maximum possible care. Advancements in virtual platform helped in consultations and academics. Delayed conservative approach was used in most cases at the expense of cosmetic compromise.
PDF
