Molecular epidemiology of koi herpesvirus Kurita, J.(National Research Inst. of Aquaculture, Tamaki, Mie (Japan). Tamaki Station); Yuasa, K; Ito, T ...
Fish Pathology,
2009, Letnik:
44, Številka:
2
Journal Article
Recenzirano
Odprti dostop
Three regions of koi herpesvirus (KHV) genomic DNA were compared for 34 samples from Japan, six from Indonesia, two from Taiwan, one from the Philippines, 13 from the Netherlands, one from the UK, ...one from the USA and one from Israel. The analyzed genomic regions included known PCR-detection targets (Sph1-5, 9/5 and the thymidine kinase gene). The KHVs from Asian countries were very homogeneous, although two variants were noted based on a single nucleotide polymorphism. In contrast, seven variants were found in KHVs from outside of Asia, and although closely related to one another, they were clearly distinct from those from Asian. The results suggest that a clear genetic distinction exists between Asian and European (including each single isolate from the USA and Israel) types of KHV, and that unique types of KHV were independently introduced or emerged in the respective geographic locations.
Virucidal activities of methanol, ethanol, 1-propanol, phenol, cresol, chlorine, iodophor and 3 kindsof invert soaps against viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder ...Paralichthys olivaceus were examined. The disinfectants were diluted with PBS (-) or artificial seawater. The virus (VHSV : JF00Ehi1) was treated with each disinfectant at different concentrations for different periods. The reaction mixtures contained fetal calf serum at a final concentration of 1%. The treated virus was then inoculated to the cultured cell line, FHM, to determine virucidal activities of the disinfectants. When the disinfectants were diluted with PBS, the virus was easily inactivated by 1-propanol, cresol, chlorine, iodophor or all invert soaps, whereas methanol, ethanol and phenol had less activities. However, when the disinfectants were diluted with artificial seawater virucidal activities of cresol and chlorine apparently decreased.
Diagnosis of koi herpesvirus (KHV) disease in Japan Sano, M.(National Research Inst. of Aquaculture, Tamaki, Mie (Japan). Inland Station); Ito, T; Kurita, J ...
Bulletin of Fisheries Research Agency (Japan),
03/2005
Journal Article
The koi herpesvirus (KHV) disease was designated as a "Specific Disease" in a Japanese law amended on June 30, 2003. The Ministry of Agriculture, Forestry and Fisheries officially announced the first ...case of KHV disease in cultured common carp Cyprinus carpio in Japan in early November 2003. At that time, the prefectural fisheries experimental stations (PFESs), which belong to local government, lacked sufficient equipment and skill required to conduct a polymerase chain reaction (PCR) tests for KHV detection. Therefore, at first, most PCR tests for KHV were carried out at the National Research Institute of Aquaculture (NRIA), Fisheries Research Agency (FRA). The NRIA gave a short training course of PCR for the research staff of PFESs in mid-November. According to the established guideline for the KHV disease, the PFESs now conduct epizootic and routine clinical examinations on diseased fish followed by PCR test to detect viral DNA in the tissues. When there is a positive reaction with the PCR test, the sample is sent to the NRIA for further confirmation by PCR. Virus isolation on the KF-1 cell line is also tried, but confirmatory diagnosis is based on the results of PCR tests. By the end of 2003, the NRIA has accepted 529 individuals of 87 cases to be diagnosed and KHV-infected carp have been found in 23 out of 47 prefectures.
PDF
