Soil salinity is a major threat to agricultural sustainability and a global food security. Until now, most research has concentrated around stomatal limitation to photosynthesis, while non-stomatal ...limitations receiving much less attention. This work summarizes the current knowledge of impact of salinity on chloroplast metabolism and operation and finding viable solutions to minimize it. The major topics covered are: (1) the key targets of the photosynthetic apparatus under salt stress; (2) a tolerance of PSII to salt stress and its repair; (3) salinity effects on biochemistry of CO
2
fixation and its regulation; (4) ionic requirements for optimal operation of chloroplasts; and (5) ion transport systems in chloroplasts that optimize chloroplast performance under hostile saline conditions. We show that enhancing plant capacity for protection by modifying PSI cyclic electron transport, redistribution of electron transport between photosystems, thylakoid membrane composition and photosynthetic antioxidant enzymes activity may be a promising way to improve tolerance to salt stress under real-field condition. It is concluded that revealing the molecular nature of chloroplast ion transporters and understanding the modes of their operation will ensure the future sustainability of the world agriculture and the prospects of biological phytoremediation of salinized land via using salt-tolerant crop germplasm.
The 2.MED1 phylogenetic branch of Yersinia pestis of the medieval biovar became widespread in the Caspian Sea region, the Caucasus, and the Northern Aral Sea region in the 20th century, causing ...outbreaks and epizootics of plague there. Some of the formed natural foci of 2.MED1 still show epizootic activity and retain their epidemic potential. In this work, we carried out a phylogenetic analysis of 46 Y. pestis strains of the medieval biovar isolated in the Caucasus, the Caspian Sea, and the Northern Aral Sea regions during epidemic outbreaks and epizootics from 1922-2014. The obtained phylogenetic data, together with epidemiological and epizootological data accumulated over a period of about a hundred years, indicate the presence of two waves of penetration of the 2.MED1 branch into the Caucasus. The first occurred, apparently, in the first half of the 20th century as a result of the penetration of 2.MED1 from the foci of the Northern and North-Western Caspian Sea. The second wave was caused by the spread of 2.MED1 from the Northern Aral to the foci of the North-Western, Northern and Eastern Caspian Sea regions at the beginning of the second half of the 20th century, followed by introduction into the Pre-Caucasus and Transcaucasia. The rapid spread of 2.MED1 could be associated with the transfer of the pathogen by land and sea transport in the process of economic activity of the population.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
This review provides an overview about recent developments and current knowledge about monitoring, generation and the functional role of reactive oxygen species (ROS) – H2O2, HO2, HO, OH−, 1O2 and ...O2− – in both oxidative degradation and signal transduction in photosynthetic organisms including microscopic techniques for ROS detection and controlled generation. Reaction schemes elucidating formation, decay and signaling of ROS in cyanobacteria as well as from chloroplasts to the nuclear genome in eukaryotes during exposure of oxygen-evolving photosynthetic organisms to oxidative stress are discussed that target the rapidly growing field of regulatory effects of ROS on nuclear gene expression.
Display omitted
•Overview about recent knowledge of ROS monitoring, generation, decay and signaling in plant cells•Exogenous fluorescence dyes, spin traps, and genetically-encoded ROS markers reviewed•Purposeful ROS production by RBOHs and signal transduction in the form of ROS waves•Reaction schemes for production of secondary ROS and inter-organelle signaling•Microscopic techniques for localized visualization and manipulation (CALI)
Flow boiling heat transfer in microchannels is used today in many diverse applications. The previous studies addressing the effect of channel size, heat flux, vapor quality, and mass flux on heat ...transfer during flow boiling are reviewed in the present paper. The relationship between flow characteristics and flow boiling heat transfer was studied experimentally for refrigerant R-C318 at moderate reduced pressures where the contribution of nucleate boiling is decisive. Flow boiling mechanisms were identified using an annular microchannel with transparent outer wall for successive visualization of boiling. The considerable suppression of nucleate boiling heat transfer was observed at transition to annular flow and explained by formation of a liquid flow with thin film and dry spots. A general equation for prediction of two-phase flow boiling heat transfer inside the circular, annular, and rectangular microchannels is proposed and verified using the experimental data. This equation accounts for the nucleate boiling suppression, forced convection, and thin film evaporative heat transfer in the form that allows to distinguish more clearly the contribution of each mechanism of heat transfer under the conditions, when it is predominant. A new approach for prediction of transition to the annular flow is proposed and verified, using the experimental data.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Human InsR, IGF1R, and IRR receptor tyrosine kinases (RTK) of the insulin receptor subfamily play an important role in signaling pathways for a wide range of physiological processes and are directly ...associated with many pathologies, including neurodegenerative diseases. The disulfide-linked dimeric structure of these receptors is unique among RTKs. Sharing high sequence and structure homology, the receptors differ dramatically in their localization, expression, and functions. In this work, using high-resolution NMR spectroscopy supported by atomistic computer modeling, conformational variability of the transmembrane domains and their interactions with surrounding lipids were found to differ significantly between representatives of the subfamily. Therefore, we suggest that the heterogeneous and highly dynamic membrane environment should be taken into account in the observed diversity of the structural/dynamic organization and mechanisms of activation of InsR, IGF1R, and IRR receptors. This membrane-mediated control of receptor signaling offers an attractive prospect for the development of new targeted therapies for diseases associated with dysfunction of insulin subfamily receptors.
The first example of (3+3)‐annulation of two different three‐membered rings is reported herein. Donor‐acceptor cyclopropanes in reaction with diaziridines were found to afford perhydropyridazine ...derivatives in high yields and diastereoselectivity under mild Lewis acid catalysis. The disclosed reaction is applicable for the broad substrate scope and exhibits an excellent functional group tolerance.
Beautiful dissymmetry: The first example of (3+3)‐annulation of two different saturated three‐membered rings is reported. Coupling of diaziridines with donor–acceptor cyclopropanes is shown to afford hexahydropyridazine derivatives. This approach can be an efficient tool for the synthesis of diverse nonsymmetric polysubstituted six‐membered rings.
UV-B causes both damage to the photosynthetic apparatus (PA) and the activation of specific mechanisms that protect the PA from excess energy and trigger a cascade of regulatory interactions with ...different photoreceptors, including phytochromes (PHYs) and cryptochromes (CRYs). However, the role of photoreceptors in plants’ responses to UV-B radiation remains undiscovered. This study explores some of these responses using tomato photoreceptor mutants (phya, phyb1, phyab2, cry1). The effects of UV-B exposure (12.3 µmol (photons) m−2 s−1) on photosynthetic rates and PSII photochemical activity, the contents of photosynthetic and UV-absorbing pigments and anthocyanins, and the nonenzymatic antioxidant capacity (TEAC) were studied. The expression of key light-signaling genes, including UV-B signaling and genes associated with the biosynthesis of chlorophylls, carotenoids, anthocyanins, and flavonoids, was also determined. Under UV-B, phyab2 and cry1 mutants demonstrated a reduction in the PSII effective quantum yield and photosynthetic rate, as well as a reduced value of TEAC. At the same time, UV-B irradiation led to a noticeable decrease in the expression of the ultraviolet-B receptor (UVR8), repressor of UV-B photomorphogenesis 2 (RUP2), cullin 4 (CUL4), anthocyanidin synthase (ANT), phenylalanine ammonia-lease (PAL), and phytochrome B2 (PHYB2) genes in phyab2 and RUP2, CUL4, ANT, PAL, and elongated hypocotyl 5 (HY5) genes in the cry1 mutant. The results indicate the mutual regulation of UVR8, PHYB2, and CRY1 photoreceptors, but not PHYB1 and PHYA, in the process of forming a response to UV-B irradiation in tomato.
A novel approach to flow injection analysis (FIA) was proposed based on the main principles of linear non-equilibrium thermodynamics (LNET). The basic principles of I. Prigogine theory for ...dissipative structures, internal entropy production rates, thermodynamic forces and fluxes arising in flow systems were shown to be applicable to FIA. The practical application of this novel FIA approach allowed the use of the extent of analytical reaction and the entropy production rates for flow system optimization, and in-depth understanding of the steady state. The FIA approach was also found to be a suitable technique for and characterizes its quality, explaining the peculiarities of short-term and long-term steady states in a FIA system and their role for reproducibility of practical measurements. The practical application o the FIA approach was found to support its theoretical principles and allow formulating an original manner to derive a basic equation in FIA theory.
Display omitted
•An approach to FIA based on linear non-equilibrium thermodynamics was proposed.•Optimization criterion based on minimum entropy production rate was suggested.•Essence of the steady state in FIA was proved.•Realization in FIA short-term and the long-term steady state was discussed.•A new approach to derive an initial equation in FIA theory was suggested.
Brassinosteroids are promising agents for alleviating the negative effects of salinity on plants, but the mechanism of their protective action is far from being understood. We investigated the effect ...of pretreatment with 24-epibrassinolide (24-EBL) on the photosynthetic and physiological parameters of potato plants under progressive salinity stress caused by root application of 100 mM NaCl. Salinity clearly inhibited primary photosynthetic processes in potato plants by reducing the contents of photosynthetic pigments, photosynthetic electron transport and photosystem II (PSII) maximal and effective quantum yields. These negative effects of salinity on primary photosynthetic processes were mainly due to toxic ionic effects on the plant’s ability to oxidize the plastoquinone pool. Pretreatment with 24-EBL alleviated this stress effect and allowed the maintenance of plastoquinone pool oxidation and the efficiency of photosystem II photochemistry to be at the same levels as those in unstressed plants; however, the pretreatment did not affect the photosynthetic pigment content. 24-EBL pretreatment clearly alleviated the decrease in leaf osmotic potential under salinity stress. The stress-induced increases in lipid peroxidation and proline contents were not changed under brassinosteroid pretreatment. However, 24-EBL pretreatment increased the peroxidase activity and improved the K
+
/Na
+
ratio in potato leaves, which were likely responsible for the protective 24-EBL action under salt stress.
This review describes the phytochrome system in higher plants and cyanobacteria and its role in regulation of photosynthetic processes and stress protection of the photosynthetic apparatus. A ...relationship between the content of the different phytochromes, the changes in the ratios of the physiologically active forms of phytochromes to their total pool and the resulting influence on photosynthetic processes is reviewed. The role of the phytochromes in the regulation of the expression of genes encoding key photosynthetic proteins, antioxidant enzymes and other components involved in stress signaling is elucidated.
Display omitted
•The phytochrome system in higher plants and cyanobacteria is described.•Influence of phytochrome system on photosynthetic processes in higher plants is analyzed.•Gene regulation by phytochromes is discussed.
PDF
