Summary
The aim of the study was to evaluate the antioxidant potential of hydroxytyrosol (HT) on human sperm quality during incubation in vitro. Semen samples collected from men attending the ...Laboratory of Histology‐Embryology of Sfax Faculty of Medicine (Tunisia) for infertility investigations were evaluated for initial sperm parameters. Only normal selected ejaculates (n = 15) were centrifuged and incubated further with or without HT (200ug ml−1) at room temperature for 45 min. After incubation, sperm motility and viability, DNA oxidation and reactive oxygen species (ROS) production were assessed. The results showed that centrifugation significantly influenced sperm motility and viability. The supplementation of HT in incubating media improved (P = 0.01) significantly sperm viability and decreased sperm DNA oxidation (P < 0.001) and ROS levels (P = 0.03) following centrifugation. It can be concluded that supplementation of HT might be helpful to maintain the human spermatozoon after centrifugation.
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•Nanosized Al-substituted NiFe2O4 were synthesized through co-precipitation method.•The Rietveld refinement revealed that the compounds have cubic spinel phase with space group ...Fd-3m.•Magnetization and coercivity field were decreased with Al substitution.•Mössbauer spectra elucidate the nature of the phases and cation distribution.•NiAl0.15Fe1.85O4 nanoparticles were showed a good photocatalytic degradation of methyl orange.
Al-substituted nickel ferrite NiAlxFe2−xO4, where x = (0.00, 0.05, 0.10 and 0.15) synthesized through chemical co-precipitation method were investigated for their structural, morphological, optical and magnetic characterization. Al3+ substitution plays a significant role in the properties of the ferrite nanoparticles. The TGA showed three mass losses, whereas DTA resulted in three endothermic peaks. Rietveld refined XRD revealed the development of single phase cubic spinel with crystallite sizes around 28–36 nm for aluminum substituted samples. TEM and SEM analysis showed the monodispersion and cubic-like nanostructure. The room temperature infra-red spectra showed the features of higher and lower energy bands detected at ν1 ∼589–595 cm−1 and ν2 ∼405–409 cm−1, respectively corresponded to octahedral vibration (O-band) and tetrahedral (T-band) complexes that also verify the creation of spinels ferrites. The Raman spectra showed five Raman active modes (A1g + Eg + 3T2g) which are estimated in the spinel structure. The optical study showed that the compounds have an optical band gap between 1.60 and 1.89 eV. Mössbauer spectra elucidate the nature of the phases and cation distribution. From the hysteresis loop, it is clear that the synthesized samples can be control, for the NiAlxFe2-xO4 nanoferrites. The photocatalytic activity of Al-substituted nickel ferrite were studied based on the degradation of methyl orange as a model compound, where the results showed that NiAl0.15Fe 1.85O4 a good photocatalytic activity.
L’objectif principal est de mesurer l’impact d’un programme d’entraînement personnalisé en créneaux sur les capacités aérobies des patients en affection de longue durée et sur leur qualité de vie.
...Étude prospective utilisant une mesure directe du VO2max avant et après 20 séances d’activités physiques individualisées. La qualité de vie est évaluée par le questionnaire MOS SF36.
Le VO2max des 153 patients inclus augmente de près de 10 % (p<0,05). La puissance développée au premier seuil ventilatoire (SV1) augmente d’environ 30 % (p<0,05) et de près de 40 % pour le sous-groupe « maladie neurodégénérative ». La qualité de vie augmente aussi de manière significative. Le protocole PEP’C améliore les capacités aérobies et la qualité de vie des patients porteurs d’une maladie chronique après 20 séances d’activités physiques basées sur la fréquence cardiaque mesurée au cours de la mesure directe du VO2max.
To measure the impact of individualized Interval training (PEP’C program) on the aerobic capacity and quality of life of patients with chronic diseases.
Prospective study by direct measurement VO2max before and after 20 sessions of individualized physical activities. Quality of life was evaluated with the MOS SF-36.
VO2max of the 153 patients increased by nearly 10% (P<0.05). Power developed at first ventilator threshold (VT1) increased by nearly 30% (P<0.05) and by nearly 40% for the subgroup with neurodegenerative diseases. Quality of life significantly improved. The PEP’C protocol improved aerobic capacity and quality of life in patients with chronic diseases after 20 sessions of physical activities based on heart rate measured during direct assessment of VO2max.
Objective: Evaluation of the reactivity of autoantibodies of systemic lupus erythematosus (SLE) patients directed against malondialdehyde (MDA)-modified catalase, superoxide dismutase (SOD), and ...different Hep2 protein fractions (hydrophobic, hydrophilic, and nuclear).
Method: Thiol groups and MDA-protein adducts were first assessed among 65 SLE patients and 60 healthy controls. Then, the reactivities of SLE immunoglobulin (Ig)G autoantibodies towards MDA-modified and unmodified proteins were compared using a standard enzyme-linked immunosorbent assay (ELISA).
Results: An increase in the levels of MDA-modified proteins and a decrease in the concentration of thiol groups among SLE patients (p < 0.05) were observed. IgG circulating autoantibodies in the sera of SLE patients exhibited a significant enhanced reactivity (p < 0.05) against catalase and SOD-modified proteins. The same data were observed in the different protein fractions extracted from cultured cells (p < 0.05).
Conclusion: These data reinforce the role of oxidative stress and especially lipid peroxidation products in the progression of SLE disease.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Objective: To evaluate the level of autoantibodies against superoxide dismutase (SOD) and catalase (CAT) in the sera of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) Tunisian ...patients, to study the oxidative profile among the same patients and to establish a correlation between the two parameters in order to understand the role of each one in the genesis of the two diseases.
Method: Using a standard enzyme-linked immunosorbent assay (ELISA), the levels of immunoglobulin G (IgG) and IgM directed against CAT and SOD in the sera of 39 RA patients, 40 SLE patients, and 50 control healthy individuals were evaluated. The oxidative antioxidative profile was tested by measuring serum malondialdehyde (MDA), conjugated dienes (CD), CAT activity, and SOD activity.
Results: Our data showed increased levels of IgG antibodies (Ab) against CAT in both groups of patients (p<0.05) compared to control subjects. However, the SLE patients displayed an increased level of anti-SOD IgG (p<0.05). In all patients the lipid peroxidation was confirmed by high levels of MDA and conjugated dienes (p<0.05). RA patients exhibited an increasing CAT and SOD activity in their sera (p<0.05) with a positive correlation observed between CAT and IgG anti-CAT (p<0.05). The same results were observed for SLE patients. In addition, a positive correlation was observed between anti-CAT Ab and anti-SOD Ab in SLE patients (p<0.05).
Conclusion: Collectively, these results suggested that the primary factor causing the oxidative stress observed in RA and SLE is excessive free radical production rather than impaired CAT or SOD activity due to autoantibody inhibition.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Background Reactive oxygen species play a key role in the development of many dermatological disorders.
Objective The purpose of this study is to examine the lipid peroxidation, protein oxidation ...and antioxidative profile in Tunisian pemphigus foliaceus (PF) patients.
Methods Malondialdehyde (MDA), conjugated dienes (CD), protein thiol levels, catalase (CAT) and superoxide dismutase (SOD) activities were evaluated in skin biopsies of 13 patients compared to biopsies of 7 healthy controls.
Results Oxidative stress was confirmed in these three types of patient biopsies as compared to controls. Thus, MDA, CD levels and catalase CAT and SOD activities were significantly increased in lesional, perilesional and normal biopsies of PF patients than in those of control subjects. Protein oxidative was confirmed by lower levels of protein thiols in lesional, perilesional and normal biopsies than in control’s biopsies. Otherwise, in patients, a significant rise of these biomarkers was observed in lesional and perilesional biopsies compared with normal biopsies.
Conclusion This study shows that oxidative stress could be involved in the pathogenesis of PF by the spread of skin lesions and/or by the increase in auto‐antibodies’ reactivity.
L’objectif de ce travail consiste à étudier les profils oxydatifs des patients tunisiens atteints de dysfonctionnements thyroïdiens. Au total, 57 (36 hypothyroïdies et 21 hyperthyroïdies) patients ...n’ayant subi aucun traitement à visée thyroïdienne et 30 sujets sains ont été colligés. Le dosage des hormones thyroïdiennes est effectué par radio-immunologie tandis que celui des anticorps antithyroïdiens par Elisa. Les dosages du malondialdéhyde, la superoxyde dismutase, la catalase et la glutathion peroxydase sont fait sur plasma par des méthodes spectrophotométriques. Une augmentation significative du malondialdéhyde a été noté chez les deux groupes de patients comparés au groupe de témoins (hyperthyroïdie p < 0.05; hypothyroïdie p < 0.01). Les deux groupes de patients ont aussi exhibé une augmentation des activités de la superoxyde dismutase et une diminution de l’activité de la glutathion peroxydase ( p < 0.05). Une augmentation de l’activité de la catalase a été notée dans les deux groupes (hypothyroïdie p < 0.01). Les hyperthyroïdiens ont montré des corrélations significatives entre le taux de la thyroxine libre et quelques paramètres du stress oxydatif ( p < 0.05); ce qui n’est pas le cas de l’hypothyroïdie. Aucune corrélation notée entre les d’anticorps anti-thyroïdiens et les paramètres du stress oxydatif. Les perturbations des activités enzymatiques anti-oxydantes chez les patients par rapport aux témoins montrent la présence du stress oxydatif au cours des hyperthyroïdies aussi bien que les hypothyroïdies. L’étude des corrélations montre l’incrimination du taux hormonale dans l’établissement de l’état de stress uniquement dans les cas d’hyperthyroïdie. Le terrain auto-immun n’est probablement pas impliqué dans le stress oxydatif observé chez les patients.
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•Ulvans from green algae Ulva lactuca were extracted by various extraction procedures.•Peculiar structures of ulvan extracts were determined by Fourier Transform Infrared and Nuclear ...Magnetic Resonance methods.•Enzymatic-chemical extract has the greatest cytotoxic activity against HeLa cancer cell lines.•Acid extracts promoted the inhibition of cancer cells and the growth of normal cells.•Acid extracts revealed the highest anticoagulant activity, in Activated Partial Thromboplastin Time and Thrombin Time assays.
The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide ‘ulvan’ from the green seaweed Ulva lactuca were investigated. The structural features of ulvans were carried out by FTIR and by one- and two- dimensional 1H and 13C NMR spectroscopic. The ulvans were mainly composed of rhamnose, xylose, and uronic acid. Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.
The extraction procedures effect were observed in chemical structure, Mw and biological activities. Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL). Acid extracts promoted to reduce HeLa cells and to grow PBL cells. At high concentrations, acid extracts showed the highest APTT and TT clotting time. Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.
L’objectif de ce travail consiste à comparer le profil oxydatif dans le cas de cancer papillaire thyroïdien à ceux de deux pathologies thyroïdiennes d’origine auto-immune. 7 cas de cancer papillaire ...thyroïdien, 29 cas de thyroïdite d’Hashimoto, 16 cas de la maladie de Basedow et 30 témoins ont fait l’objet de cette étude. Les dosages suivants ont été effectués sur plasma : thyroxine et tri-iodothyronine libres (RIA), anticorps anti-thyroperoxydase et anticorps anti-thyroglobuline (ELISA), malondialdéhyde, superoxyde dismutase, catalase et glutathion peroxydase (spectrophotométrie). Nos résultats ont montré une augmentation du taux de malondialdéhyde et de l’activité de la superoxyde dismutase chez les patients atteint de cancer papillaire thyroïdien par rapport aux patients atteints de la maladie de Basedow ( p < 0,01 ; p < 0,001, respectivement). Comparé aux cas de la thyroïdite d’Hashimoto, ceux du cancer papillaire thyroïdien ont montré une augmentation significative du taux du malondialdéhyde et des activités de la superoxyde dismutase et la catalase et une diminution significative de l’activité de la glutathion peroxydase ( p < 0,01 ; p < 0,001 ; p < 0,05 ; p < 0,05 respectivement). Aucune différence significative n’a été notée entre la maladie de Basedow et le cancer papillaire thyroïdien. Le profil oxydatif semble être fortement perturbé dans le cas de cancer papillaire thyroïdien par rapport à ceux des pathologies auto-immunes. Des études complémentaires sont nécessaires pour déterminer si le stress oxydatif a une valeur pronostique dans le cancer papillaire thyroïdien ou non.
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