There is substantial inter-individual diversity in the susceptibility of alcoholics to liver injury. Alterations of intestinal microbiota (IM) have been reported in alcoholic liver disease (ALD), but ...the extent to which they are merely a consequence or a cause is unknown. We aimed to demonstrate that a specific dysbiosis contributes to the development of alcoholic hepatitis (AH).
We humanised germ-free and conventional mice using human IM transplant from alcoholic patients with or without AH. The consequences on alcohol-fed recipient mice were studied.
A specific dysbiosis was associated with ALD severity in patients. Mice harbouring the IM from a patient with severe AH (sAH) developed more severe liver inflammation with an increased number of liver T lymphocyte subsets and Natural Killer T (NKT) lymphocytes, higher liver necrosis, greater intestinal permeability and higher translocation of bacteria than mice harbouring the IM from an alcoholic patient without AH (noAH). Similarly, CD45+ lymphocyte subsets were increased in visceral adipose tissue, and CD4(+)T and NKT lymphocytes in mesenteric lymph nodes. The IM associated with sAH and noAH could be distinguished by differences in bacterial abundance and composition. Key deleterious species were associated with sAH while the Faecalibacterium genus was associated with noAH. Ursodeoxycholic acid was more abundant in faeces from noAH mice. Additionally, in conventional mice humanised with the IM from an sAH patient, a second subsequent transfer of IM from an noAH patient improved alcohol-induced liver lesions.
Individual susceptibility to ALD is substantially driven by IM. It may, therefore, be possible to prevent and manage ALD by IM manipulation.
We describe a revised collection of the number of sunspot groups from 1610 to the present. This new collection is based on the work of Hoyt and Schatten (
Solar Phys
.
179
, 189,
1998
). The main ...changes are the elimination of a considerable number of observations during the Maunder Minimum (hereafter, MM) and the inclusion of several long series of observations. Numerous minor changes are also described. Moreover, we have calculated the active-day percentage during the MM from this new collection as a reliable index of the solar activity. Thus, the level of solar activity obtained in this work is greater than the level obtained using the original Hoyt and Schatten data, although it remains compatible with a grand minimum of solar activity. The new collection is available in digital format.
Polyamines on the Reproductive Landscape Lefèvre, Pavine L. C; Palin, Marie-France; Murphy, Bruce D
Endocrine reviews,
2011-October, Letnik:
32, Številka:
5
Journal Article
Recenzirano
Odprti dostop
The polyamines are ubiquitous polycationic compounds. Over the past 40 yr, investigation has shown that some of these, namely spermine, spermidine, and putrescine, are essential to male and female ...reproductive processes and to embryo/fetal development. Indeed, their absence is characterized by infertility and arrest in embryogenesis. Mammals synthesize polyamines de novo from amino acids or import these compounds from the diet. Information collected recently has shown that polyamines are essential regulators of cell growth and gene expression, and they have been implicated in both mitosis and meiosis. In male reproduction, polyamine expression correlates with stages of spermatogenesis, and polyamines appear to function in promoting sperm motility. There is evidence for polyamine involvement in ovarian follicle development and ovulation in female mammals, and polyamine synthesis is required for steroidogenesis in the ovary. Studies of the embryo indicate a polyamine requirement that can be met from maternal sources before implantation, whereas elimination of polyamine synthesis abrogates embryo development at gastrulation. Polyamines play roles in embryo implantation, in decidualization, and in placental formation and function, and polyamine privation during gestation results in intrauterine growth retardation. Emerging information implicates dietary arginine and dietary polyamines as nutritional regulators of fertility. The mechanisms by which polyamines regulate these multiple and diverse processes are not yet well explored; thus, there is fertile ground for further productive investigation.
We report progress on the ongoing recalibration of the Wolf sunspot number (SN) and group-sunspot number (GN) following the release of version 2.0 of SN in 2015. This report constitutes both an ...update of the efforts reported in the 2016 Topical Issue of Solar Physics and a summary of work by the International Space Science Institute (ISSI) International Team formed in 2017 to develop optimal SN and GN reconstruction methods while continuing to expand the historical sunspot-number database. Significant progress has been made on the database side while more work is needed to bring the various proposed SN and (primarily) GN reconstruction methods closer to maturity, after which the new reconstructions (or combinations thereof) can be compared with (a) “benchmark” expectations for any normalization scheme (e.g., a general increase in observer normalization factors going back in time), and (b) independent proxy data series such as F10.7 and the daily range of variations of Earth’s undisturbed magnetic field. New versions of the underlying databases for SN and GN will shortly become available for years through 2022 and we anticipate the release of the next versions of these two time series in 2024.
In 1876, Alfred Wolfer started observing the Sun and recording the properties of sunspots alongside Rudolf Wolf. Their observations became the basis for the construction of the sunspot-number series. ...After Wolf’s death in 1893, Wolfer became the primary observer for the sunspot-number series. Even though the observations of Wolf and Wolfer had an overlap of almost 17 years (1876–1893), this shift of primary observer from Wolf to Wolfer seems to have led to inconsistencies in the sunspot-number series, primarily due to inhomogeneities in Wolf’s observations. To address this issue we digitise Mittheilungen (Wolf’s journals) and analyse their tabulated datasets. These journals include the raw sunspot data from various observers that the Zürich Observatory used to compile the sunspot number series (SNV1). These datasets have been used as source data for the construction of the first version of the sunspot number (SNV1) series, but they were not digitally accessible for a recalibration of the sunspot-number series until recently. We have also acquired external datasets from recent archival investigations for contemporaneous sunspot observations. In this study, we use the Mittheilungen dataset to produce a new recalibration of the sunspot-number series covering 1816–1944, using four major observers (Tevel, Schwabe, Weber and Wolfer) as backbones. The availability of the raw data allows us to identify issues in the determination of the scaling factors or
k
-factors, between the records of different observers, but also the use of modern techniques for cross-calibrations. Our reconstruction for the years 1816–1944 is carried out with a novel method inspired by Chatzistergos et al. (
Astron. Astrophys
.
602
, A69,
2017
) allowing us to eliminate inconsistencies that resulted from the application of erroneous
k
-factors.
The Maunder Minimum (MM) was a prolonged period of low solar activity that occurred between 1645 and 1715. The true level of solar activity corresponding to this epoch is still a matter of debate. In ...order to compare solar activity during the MM with that of other epochs, we have evaluated the umbra-penumbra area ratio (U/P hereafter) during the MM. Thus, we have analyzed 196 sunspot drawings, including 48 different sunspots observed during the period 1660-1709. The mode value of the ratio obtained from the occurrence frequency distribution lies between 0.15 and 0.25. Furthermore, the median and mean values are equal to 0.24 0.07 and 0.27 0.08 with a sigma clipping, respectively. These results are consistent with recent research using more modern data. Higher U/P values mean faster sunspot decay rates. From our results, the absence of sunspots during the MM could not be explained by changes in the U/P since the values of the ratio obtained in this work are similar to values found for other epochs.
Sunspot catalogs are very useful for studying the solar activity of the recent past. In this context, a catalog covering more than three solar cycles made by the astronomers of the Madrid ...Astronomical Observatory in Spain (nowadays, the National Astronomical Observatory) from 1952 until 1986 has been recovered. Moreover, a machine-readable version of this catalog has been made available. We have recovered abundant metadata and studied the reliability of this dataset by comparing it with other sunspot catalogs.
Polyamine deprivation maintains embryos in embryonic diapause and induces a delay of the timing of embryo implantation; polyamines are essential to reactivation of embryo in obligate diapause.
...Embryonic diapause is a poorly understood phenomenon of reversible arrest of embryo development prior to implantation. In many carnivores, such as the mink (Neovison vison), obligate diapause characterizes each gestation. Embryo reactivation is controlled by the uterus by mechanisms that remain elusive. Because polyamines are essential regulators of cell proliferation and growth, it was hypothesized that they trigger embryo reactivation. To test this, mated mink females were treated with α-difluoromethylornithine, an inhibitor of ornithine decarboxylase 1, the rate-limiting enzyme in polyamine biosynthesis, or saline as a control during the first 5 d of reactivation. This treatment induced polyamine deprivation with the consequence of rearrest in embryo cell proliferation. A mink trophoblast cell line in vitro subjected to α-difluoromethylornithine treatment likewise displayed an arrest in cell proliferation, morphological changes, and intracellular translocation of ornithine decarboxylase 1 protein. The arrest in embryo development deferred implantation for a period consistent with the length of treatment. Successful implantation and parturition ensued. We conclude that polyamine deprivation brought about a reversible rearrest of embryo development, which returned the mink embryo to diapause and induced a second delay in embryo implantation. The results are the first demonstration of a factor essential to reactivation of embryos in obligate diapause.
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