The use of environmental tracers brings comprehensive benefits to the management of water resources since it helps to prevent their pollution, minimize public health risks, and thus reduce the impact ...of urbanization. In Brazil, the Guarani Aquifer System (GAS) has strategic and environmental importance, making its preservation and sustainable exploitation mandatory. The present study aimed at evaluating sources of contamination in the GAS using the combination of geochemical data and two environmental tracers: nitrate isotopes (
15
N
NO3
and
18
O
NO3
) and one rare earth element (Gadolinium—Gd). For that, five wells—four exploiting the GAS and one the Bauru Aquifer System (BAS)—were selected to discuss the human inputs in groundwater used for public supply in an urban area. Traditional physicochemical analyses were conducted for six campaign samplings and nitrate monitoring for this period was evaluated on a time scale, also considering the accumulated rainfall. Besides that, the double isotopic method (DIM), e.g., δ
18
O
NO3
e δ
15
N
NO3
, was applied to identify the fractionation and enable the distinction of the nitrate contamination source. In addition, the determination of anomalies of Gd, a wastewater-derived contaminant, was also performed to verify recent human inputs in groundwater. The results show that the local existence of nitrate in the GAS and BAS—even at low concentrations (values from 0.26 to 6.68 mg L
−1
)—originated from anthropogenic inputs (septic waste), as indicates the typical isotopic signals ratio in the isotopic approach. Associated with that, the evaluation of Gd permitted the separation of groundwater samples into older or more recent leakages. The use of environmental tracers to assess anthropogenic inputs in groundwater reiterates the importance of adopting more effective protection strategies for water resources management systems, in order to prevent contamination.
Graphical Abstract
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•Inadequate waste disposal areas (n = 104) in Brazil were assessed for contamination.•Most studies were published in Portuguese, undermining dissemination.•Contamination of ...groundwater and surface water was observed in most studies (>70%)•Coliforms, biochemical oxygen demand, and lead were frequently above Brazilian legislation.•Most dumpsites have insignificant or low vulnerability to groundwater contamination.
This work presents an overview of environmental studies performed in areas affected by dumpsites and non-sanitary landfills in Brazil, focusing on physicochemical analysis of surface water, groundwater, and soils, as well as geophysical surveys. The objective was to identify the main contaminants found in these areas and to assess their environmental impact, guiding possible intervention measures, indicating the priority areas, and showing whether post-closure activities are mandatory. The results should support governmental actions, especially considering the recent approval of Brazil’s new sanitation framework. The present review assessed 162 publications, which described 104 different municipal solid waste (MSW) disposal sites. The physicochemical parameters of analyzed surface water, groundwater, and soil samples were above Brazilian legislation levels in 74%, 70%, and 24% of the studies, respectively. The parameters more frequently above permissible levels were coliforms, biochemical oxygen demand, dissolved oxygen, and phosphorus for surface water; lead, coliforms, and iron for groundwater; copper, cadmium, lead, and zinc for soil. The results indicated that Brazilian dumpsites are causing negative impacts in surrounding soils and water resources, posing ecological and public health risks that persist even after the site’s closure. Considering that this study provides only a reduced scenario, the dimension of this environmental problem in Brazil is noteworthy. Therefore, it is suggested that not only inadequate disposal is prohibited, but also efficient enforcement methods are adopted and aftercare is appropriately regulated in the country, making it obligatory to establish monitoring plans and implement mitigation techniques to avoid abandoning potentially contaminated areas.
hydrological monitoring is essential for a proper decision-making process and modelling. Efforts have been made in Brazil to carry out field activities at the basin scale, but how complete and ...comprehensive are those studies? Where are they located? How long have they been carried out? What are the main findings? To answer these questions, an overview of experimental monitoring basins in Brazil is presented, listing their geographical locations, monitored variables, operational status, monitoring periods and main publications. We identified 60 monitored sites, spread across most Brazilian biomes, with an average monitoring period of 12 years. However, some publications from these monitoring studies are not fully accessible to the international community. Field hydrology activities in Brazil contribute to a better understanding of hydrological processes in humid and semi-arid regions and inform us about the impacts of land-use change on water quality and quantity.
BACKGROUND: Ergonomics in design is intended to close the gap between what is designed and the real work. This study discusses the creation of technical ergonomics requirements for the basic design ...stage of workshops on offshore platforms. OBJECTIVE: This paper intends to show how the ergonomics discipline contributes to integrating knowledge on the real work of the maintenance crew with the design. METHODS: This case study uses procedures, data collection, and analysis guided by the Ergonomics of the Activity’s (EA) theoretical framework, focused on design projects. RESULTS: The results show how ergonomics contributed to a better understanding of maintenance work, as well as how this knowledge was integrated into the project design. This occurred both through the construction of a new layout and equipment list, and through the development of technical specifications. These products enable greater operational efficiency, reductions in the costs of alterations in the next stages of the project, and improvement in working conditions. CONCLUSIONS: This work points to the need for new studies on platform maintenance work, in addition to studies that deepen the debate on consolidating ergonomics practice in design projects.
Recent research has shown the need for an in-depth knowledge of zinc biofortification of cereal and oilseed grains due to its importance to human nutrition. However, little is known about the Zn ...dynamics in plant-soil system. In this work, we evaluated the effect of soil-applied Zn on the absorption, translocation, and compartmentalization of Zn in rice (Oryza sativa L.) and soybean (Glycine max L. Merrill) plants. The soil used in the greenhouse experiment was fertilized with zinc chloride (ZnCl
2
) at rates of 0, 1, 2, 4, and 8 mg Zn kg
−1
. The source of Zn was labeled by
65
Zn with specific activity of 185.5 kBq mg
−1
Zn. The amount of Zn derived from fertilizer and its use in each plants compartment was determined by direct method in isotopic calculations. Rice and soybean plants presented low efficiency in the absorption from soil-applied Zn. The accumulated Zn in the panicle, pod, and grains was not modified, due to its low translocation in the plant. The Zn uptake in rice plants was from 1.34 to 4.60 mg pot
−1
in shoots and just 0.81 to 1.43 mg pot
−1
translocated to panicles. Soybean plants presented Zn uptake between 2.36 and 4.68 mg pot
−1
in shoots, out of which 0.19 to 0.34 mg.pot
−1
and 0.48 to 0.57 mg pot
−1
translocated to grains and pods, respectively. The nutrient utilization from fertilizer was low, with mean values of 12 and 8.7% for rice and soybean plants, respectively. Soil-applied Zn showed low capacity for enriching rice panicle and soybean pod or grain probably due low Zn uptake and translocation.
BACKGROUND: Ergonomics is increasingly gaining ground in projects in the energy sector, as well as in the continuous process industry, although the discipline is still framing its space in this ...milieu. This issue emerges from the designers’ lack of familiarity with ergonomics work, especially in engineering design projects, combined with the usual expectations of organizations regarding the participation of ergonomists and the deliverables they ought to develop throughout the project. OBJECTIVE: This study aims to report the participation of a team of ergonomists in an oil platform basic design, discussing ergonomics structuring as an emerging design discipline. METHODS: This qualitative study used participant observation as a methodology approach aligned with the theoretical framework of Activity Ergonomics (AE). The data collection occurred between October 2018 and June 2020 in Rio de Janeiro, Brazil. RESULTS: The results show the challenges faced by the ergonomics team and strategies adopted in order to create technical specifications (TS), which will determine the work conditions in the future platforms. An evolution in relation to the practice of Ergonomics during this project can be pointed out, even though part of its scope of action remains to be better understood among the design team. CONCLUSION: Considering oil platform project contexts, this work highlights how ergonomics can help integrate the different rationalities that compose the design process.
Paclitaxel is an antineoplastic agent widely used to treat several solid tumor types. The primary mechanism of action of paclitaxel is based on microtubule stabilization inducing cell-cycle arrest. ...Here, we use several tumor models to show that paclitaxel not only induces tumor cell-cycle arrest, but also promotes antitumor immunity.
, paclitaxel reprogrammed M2-polarized macrophages to the M1-like phenotype in a TLR4-dependent manner, similarly to LPS. Paclitaxel also modulated the tumor-associated macrophage (TAM) profile in mouse models of breast and melanoma tumors; gene expression analysis showed that paclitaxel altered the M2-like signature of TAMs toward an M1-like profile. In mice selectively lacking TLR4 on myeloid cells, for example, macrophages (LysM-Cre
/TLR4
), the antitumor effect of paclitaxel was attenuated. Gene expression analysis of tumor samples from patients with ovarian cancer before and after treatment with paclitaxel detected an enrichment of genes linked to the M1 macrophage activation profile (IFNγ-stimulated macrophages). These findings indicate that paclitaxel skews TAMs toward an immunocompetent profile via TLR4, which might contribute to the antitumor effect of paclitaxel and provide a rationale for new combination regimens comprising paclitaxel and immunotherapies as an anticancer treatment.
This study provides new evidence that the antitumor effect of paclitaxel occurs in part via reactivation of the immune response against cancer, guiding tumor-associated macrophages toward the M1-like antitumor phenotype.
http://cancerres.aacrjournals.org/content/canres/78/20/5891/F1.large.jpg
.
Abstract
In the present study, it was sought to compare yeast microbiota of wild and captive Macrobrachium amazonicum and evaluate the antifungal susceptibility and production of virulence factors by ...the recovered isolates of Candida spp. Additionally, cultivation water was monitored for the presence of fungi. Overall, 26 yeast isolates belonging to three genera and seven species were obtained, out of which 24 were Candida spp., with Candida famata as the most prevalent species for both wild and captive prawns. From cultivation water, 28 isolates of filamentous fungi were obtained, with Penicillium spp., Cladosporium spp. and Aspergillus spp. as the most frequent genera. Eight out of 24 Candida spp. isolates were resistant to azole derivatives, out of which four were recovered from wild-harvested prawns. As for production of virulence factors, three (12.5%) and eight (33.3%) isolates presented phospholipase and protease activity, respectively. This is the first comparative study between wild and captive prawns and the first report on yeast microbiota of M. amazonicum. The most relevant finding was the high percentage of resistant Candida spp., including from wild individuals, which suggests the occurrence of an environmental imbalance in the area where these prawns were captured.
Deficits of postural control and perceptions of verticality are disabling problems observed in stroke patients that have been recently correlated to each other. However, there is no evidence in the ...literature confirming this relationship with quantitative posturography analysis. Therefore, the objectives of the present study were to analyze the relationship between Subjective Postural Vertical (SPV) and Haptic Vertical (HV) with posturography and functionality in stroke patients. We included 45 stroke patients. The study protocol was composed by clinical interview, evaluation of SPV and HV in roll and pitch planes and posturography. Posturography was measured in the sitting and standing positions under the conditions: eyes open, stable surface (EOSS); eyes closed, stable surface (ECSS); eyes open, unstable surface (EOUS); and eyes closed, unstable surface (ECUS). The median PV in roll plane was 0.34° (-1.44° to 2.54°) and in pitch plane 0.36° (-2.72° to 2.45°). The median of HV in roll and pitch planes were -0.94° (-5.86° to 3.84°) and 3.56° (-0.68° to 8.36°), respectively. SPV in the roll plane was correlated with all posturagraphy parameters in sitting position in all conditions (r = 0.35 to 0.47; p < 0.006). There were moderate correlations with the verticality perceptions and all the functional scales. Linear regression model showed association between speed and SPV in the roll plane in the condition EOSS (R2 of 0.37; p = 0.005), in the condition ECSS (R2 of 0.13; p = 0.04) and in the condition EOUS (R2 of 0.22; p = 0.03). These results suggest that verticality perception is a relevant component of postural control and should be systematically evaluated, particularly in patients with abnormal postural control.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Summary
Sound evidence supports a role for interleukin‐17 (IL‐17) ‐producing γδ T cells and IL‐17‐producing helper T (Th17) cells in intestinal homeostasis, especially in intestinal barrier ...integrity. In the present study, we aimed to evaluate the role of IL‐17 cytokine in the regulation of intestinal immunity and obesity‐induced metabolic syndrome (MetS) in an experimental murine model. C57BL/6 wild‐type (WT) mice and mice lacking the IL‐17 cytokine receptor (IL‐17RA−/−) were fed either a control diet (CD) or a high‐fat diet (HFD) for 9 weeks. Our data demonstrate that IL‐17RA−/− mice are protected against obesity, but develop hyperglycemia, hyperinsulinemia and insulin resistance. In parallel, HFD‐fed IL‐17RA−/− mice display intense inflammation in the ileum compared with WT mice on the HFD. IL‐17RA−/− mice fed the HFD exhibit impaired neutrophil migration to the intestinal mucosa and reduced gene expression of the CXCL‐1 chemokine and CXCR‐2 receptor in the ileum. Interestingly, the populations of neutrophils (CD11b+ Ly6G+) and anti‐inflammatory macrophages (CD11b+ CX3CR1+) are increased in the mesenteric lymph nodes of these mice. IL‐17RA−/− mice on the HFD also display increased commensal bacterial translocation into the bloodstream and elevated lipopolysaccharide (LPS) levels in the visceral adipose tissue (VAT). Metagenomic analysis of bacterial 16S gene revealed increased Proteobacteria and Bacteroidetes phyla, the main representatives of Gram‐negative bacteria, and reduced Akkermansia muciniphila in the fecal samples of IL‐17RA−/− mice fed the HFD. Together, these data indicate that the IL‐17/IL‐17R axis drives intestinal neutrophil migration, limits gut dysbiosis and attenuates LPS translocation to VAT, resulting in protection to MetS.
Deficiency of the gene encoding the interleukin‐17 cytokine receptor (IL‐17RA) in mice protected against high‐fat diet (HFD) ‐induced obesity, but promoted hyperglycemia, glucose intolerance and insulin resistance. Additionally, IL‐17RA−/− mice exhibited intense intestinal inflammation associated with defective neutrophil migration to intestinal mucosa and decreased expression of the chemokine (C‐X‐C motif) ligand‐1 (CXCL‐1) in the ileum. Importantly, IL‐17RA−/− mice had augmented abundance of Proteobacteria and Bacteroidetes phyla in the gut microbiota, increased intestinal permeability and increased lipopolysaccharide (LPS) levels in the visceral adipose tissue (VAT). Overall, IL‐17 cytokine confers resistance to metabolic syndrome by inducing neutrophil migration to the intestinal mucosa through CXCL‐1, limiting gut dysbiosis and controlling LPS translocation to the VAT.
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