Abstract
The fabrication of macroscopic objects from covalent organic frameworks (COFs) is challenging but of great significance to fully exploit their chemical functionality and porosity. Herein, ...COF/reduced graphene oxide (rGO) aerogels synthesized by a hydrothermal approach are presented. The COFs grow in situ along the surface of the 2D graphene sheets, which are stacked in a 3D fashion, forming an ultralight aerogel with a hierarchical porous structure after freeze-drying, which can be compressed and expanded several times without breaking. The COF/rGO aerogels show excellent absorption capacity (uptake of >200 g organic solvent/g aerogel), which can be used for removal of various organic liquids from water. Moreover, as active material of supercapacitor devices, the aerogel delivers a high capacitance of 269 F g
−1
at 0.5 A g
−1
and cycling stability over 5000 cycles.
Anthocyanins act as polyphenolic pigment that is ubiquitously found in plants. Anthocyanins play a role not only in health-promoting as an antioxidant, but also in protection against all kinds of ...abiotic and biotic stresses. Most recent studies have found that MYB transcription factors (MYB TFs) could positively or negatively regulate anthocyanin biosynthesis. Understanding the roles of MYB TFs is essential in elucidating how MYB TFs regulate the accumulation of anthocyanin. In the review, we summarized the signaling pathways medicated by MYB TFs during anthocyanin biosynthesis including jasmonic acid (JA) signaling pathway, cytokinins (CKs) signaling pathway, temperature-induced, light signal, 26S proteasome pathway, NAC TFs, and bHLH TFs. Moreover, structural and regulator genes induced by MYB TFs, target genes bound and activated or suppressed by MYB TFs, and crosstalk between MYB TFs and other proteins, were found to be vitally important in the regulation of anthocyanin biosynthesis. In this study, we focus on the recent knowledge concerning the regulator signaling and mechanism of MYB TFs on anthocyanin biosynthesis, covering the signaling pathway, genes expression, and target genes and protein expression.
Nitric oxide (NO) acts as a gaseous signalling molecule and is considered to be a key regulator in the postharvest storage of fruits. Postharvest senescence is one of the most serious threats ...affecting the usage and economic value of fruits. Most recent studies have found that exogenous NO application can effectively improve the quality and prolong the shelf life of fruit postharvest by inhibiting postharvest diseases and alleviating chilling injury. Understanding the roles of NO is essential to elucidating how NO activates the appropriate set of responses to postharvest senescence. Here, we concluded that exogenous NO treatment alleviated senescence in postharvest fruit and attributed this to the following factors: (1) ethylene biosynthesis, (2) the antioxidant system, (3) polyamine metabolism and γ-aminobutyric acid (GABA) shunting, (4) cell wall metabolism, (5) sugar metabolism, (6) energy metabolism, (7) the CRT/DRE-binding factor (CBF) pathway and (8) S-nitrosylation. Moreover, crosstalk between NO and hydrogen sulfide (H2S), hydrogen peroxide (H2O2), oxalic acid (OA), arginine (Arg), GATA or plant hormone abscisic acid (ABA), melatonin (MT), and methyl jasmonate (MeJA), along with the regulation of key genes, were found to be very important in responses to postharvest senescence. In this study, we focus on the recent knowledge concerning the alleviative effect of NO on postharvest senescence, covering ethylene biosynthesis, the antioxidant system and related gene and protein expression.
Abstract
When new covalent organic frameworks (COFs) are designed, the main efforts are typically focused on selecting specific building blocks with certain geometries and properties to control the ...structure and function of the final COFs. The nature of the linkage (imine, boroxine, vinyl, etc.) between these building blocks naturally also defines their properties. However, besides the linkage type, the orientation,
i.e
., the constitutional isomerism of these linkages, has rarely been considered so far as an essential aspect. In this work, three pairs of constitutionally isomeric imine-linked donor-acceptor (D-A) COFs are synthesized, which are different in the orientation of the imine bonds (D-C=N-A (DCNA) and D-N=C-A (DNCA)). The constitutional isomers show substantial differences in their photophysical properties and consequently in their photocatalytic performance. Indeed, all DCNA COFs show enhanced photocatalytic H
2
evolution performance than the corresponding DNCA COFs. Besides the imine COFs shown here, it can be concluded that the proposed concept of constitutional isomerism of linkages in COFs is quite universal and should be considered when designing and tuning the properties of COFs.
Previous results have shown that hydrogen peroxide (H2O2) is involved in abscisic acid (ABA)-induced adventitious root development under drought stress. In this study, a comparative proteomic ...analysis was conducted to explore the key proteins during ABA-H2O2-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress. The results revealed that 48 of 56 detected proteins spots were confidently matched to NCBI database entries. Among them, 10 protein spots were up-regulated while 4 protein spots were down-regulated under drought stress; 22 protein spots were up-regulated by ABA under drought stress; treatment with ABA plus H2O2 scavenger catalase (CAT) up-regulated 6 protein spots and down-regulated 6 protein spots under drought stress. The identified proteins were divided into three categories: biological process, molecular function, and cellular component. According to their functions, the 48 identified proteins were grouped into 10 categories, including photosynthesis, stress response, protein folding, modification, and degradation, etc. According to subcellular localization, about 24 proteins (half of the total) were predicted to be localized in chloroplasts. ABA significantly up-regulated the expression of photosynthesis-related proteins (SBPase, OEE1), stress-defense-related proteins (2-Cys-Prx, HBP2), and folding-, modification-, and degradation-related proteins (TPal) under drought stress. However, the effects of ABA were inhibited by CAT. The proteins were further analyzed at the transcription level, and the expression of four of five genes (except 2-Cys-Prx) was in accordance with the corresponding protein expression. The protein abundance changes of OEE1 and SBPase were also supported by western blot analysis. Therefore, H2O2 may be involved in ABA-induced adventitious root development under drought stress by regulating photosynthesis-related proteins, stress defense-related proteins, and folding-, modification-, and degradation-related proteins.
Well-intergrown nanocrystals of zeolitic imidazolate frameworks (ZIF-8) supported on three-dimensional (3D) graphene were prepared by a counter diffusion technique. The incorporation of ZIF-8 ...crystals greatly improves the surface areas of the graphene composites. The carbonized graphene-ZIF composites with hierarchical pore structures showed high electrochemical capacitance and good stability. This work provides an efficient method to synthesize porous carbon materials with high capacitance.
Melatonin (MT) and nitric oxide (NO) act as signaling molecules that can enhance cadmium (Cd) stress resistance in plants. However, little information is available about the relationship between MT ...and NO during seedling growth under Cd stress. We hypothesize that NO may be involved in how MT responds to Cd stress during seedling growth. The aim of this study is to evaluate the relationship and mechanism of response. The results indicate that different concentrations of Cd inhibit the growth of tomato seedlings. Exogenous MT or NO promotes seedling growth under Cd stress, with a maximal biological response at 100 μM MT or NO. The promotive effects of MT-induced seedling growth under Cd stress are suppressed by NO scavenger 2-4-carboxyphenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (cPTIO), suggesting that NO may be involved in MT-induced seedling growth under Cd stress. MT or NO decreases the content of hydrogen peroxide (H
O
), malonaldehyde (MDA), dehydroascorbic acid (DHA), and oxidized glutathione (GSSG); improves the content of ascorbic acid (AsA) and glutathione (GSH) and the ratios of AsA/DHA and GSH/GSSG; and enhances the activities of glutathione reductase (GR), monodehydroascorbic acid reductase (MDHAR), dehydroascorbic acid reductase (DHAR), ascorbic acid oxidase (AAO), and ascorbate peroxidase (APX) to alleviate oxidative damage. Moreover, the expression of genes associated with the ascorbate-glutathione (AsA-GSH) cycle and reactive oxygen species (ROS) are up-regulated by MT or NO under Cd conditions, including
,
,
,
,
,
,
, and
. However, NO scavenger cPTIO reverses the positive effects regulated by MT. The results indicate that MT-mediated NO enhances Cd tolerance by regulating AsA-GSH cycle and ROS metabolism.
Methane (CH
) and brassinosteroids (BRs) are important signaling molecules involved in a variety of biological processes in plants.
Here, marigold (Tagetes erecta L. 'Marvel') was used to investigate ...the role and relationship between CH
and BRs during adventitious root (AR) formation. The results showed a dose-dependent effect of CH
and BRs on rooting, with the greatest biological effects of methane-rich water (MRW, CH
donor) and 2,4-epibrassinolide (EBL) at 20% and 1 μmol L
, respectively. The positive effect of MRW on AR formation was blocked by brassinoazole (Brz, a synthetic inhibitor of EBL), indicating that BRs might be involved in MRW-regulated AR formation. MRW promoted EBL accumulation during rooting by up-regulating the content of campestanol (CN), cathasterone (CT), and castasterone (CS) and the activity of Steroid 5α-reductase (DET2), 22α-hydroxylase (DWF4), and BR-6-oxidase (BR6ox), indicating that CH
could induce endogenous brassinolide (BR) production during rooting. Further results showed that MRW and EBL significantly down-regulated the content of cellulose, hemicellulose and lignin during rooting and significantly up-regulated the hydrolase activity, i.e. cmcase, xylanase and laccase. In addition, MRW and EBL also significantly promoted the activity of two major cell wall relaxing factors, xyloglucan endotransglucosylase/hydrolase (XTH) and peroxidase, which in turn promoted AR formation. While, Brz inhibited the role of MRW on these substances.
BR might be involved in CH
-promoted AR formation by increasing cell wall relaxation.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abscisic acid (ABA) and nitric oxide (NO), as unique signaling molecules, are involved in plant growth, developmental processes, and abiotic stresses. However, the interaction between ABA and NO ...under abiotic stresses has little been worked out at present. Therefore, this paper reviews the mechanisms of crosstalk between ABA and NO in the regulation of plants in response to environmental stresses. Firstly, ABA-NO interaction can alleviate the changes of plant morphological indexes damaged by abiotic stresses, for instance, root length, leaf area, and fresh weight. Secondly, regulatory mechanisms of interaction between ABA and NO are also summarized, such as reactive oxygen species (ROS), antioxidant enzymes, proline, flavonoids, polyamines (PAs), ascorbate-glutathione cycle, water balance, photosynthetic, stomatal movement, and post-translational modifications. Meanwhile, the relationships between ABA and NO are established. ABA regulates NO through ROS at the physiological level during the regulatory processes. At the molecular level, NO counteracts ABA through mediating post-translational modifications. Moreover, we also discuss key genes related to the antioxidant enzymes, PAs biosynthesis, ABA receptor, NO biosynthesis, and flavonoid biosynthesis that are regulated by the interaction between ABA and NO under environmental stresses. This review will provide new guiding directions for the mechanism of the crosstalk between ABA and NO to alleviate abiotic stresses.
Nitric oxide (NO), as a ubiquitous gas signaling molecule, modulates various physiological and biochemical processes and stress responses in plants. In our study, the NO donor nitrosoglutathione ...(GSNO) significantly promoted tomato seedling growth under NaCl stress, whereas NO scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide potassium (cPTIO) treatment reversed the positive effect of NO, indicating that NO plays an essential role in enhancing salt stress resistance. To explore the mechanism of NO-alleviated salt stress, the transcriptome of tomato leaves was analyzed. A total of 739 differentially expressed genes (DEGs) were identified and classified into different metabolic pathways, especially photosynthesis, plant hormone signal transduction, and carbon metabolism. Of these, approximately 16 and 9 DEGs involved in plant signal transduction and photosynthesis, respectively, were further studied. We found that GSNO increased the endogenous indoleacetic acid (IAA) and salicylic acid (SA) levels but decreased abscisic acid (ABA) and ethylene (ETH) levels under salt stress conditions. Additionally, GSNO induced increases in photosynthesis pigment content and chlorophyll fluorescence parameters under NaCl stress, thereby enhancing the photosynthetic capacity of tomato seedlings. Moreover, the effects of NO mentioned above were reversed by cPTIO. Together, the results of this study revealed that NO regulates the expression of genes related to phytohormone signal transduction and photosynthesis antenna proteins and, therefore, regulates endogenous hormonal equilibrium and enhances photosynthetic capacity, alleviating salt toxicity in tomato seedlings.
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