For the spaceborne synthetic aperture radar (SAR), it is difficult to obtain high resolution and wide width at the same time. This paper proposes a novel imaging system based on tandem SAR ...satellites, where one obtains coarse resolution and wide swath by the scanning mode, and the other obtains the undersampled echo from the same swath. The high resolution is achieved by associating the tandem SARs’ echo and using the minimum-energy-based algorithm. Finally, a high-resolution wide-swath SAR system is designed, and its imaging performance is verified by simulated data and real airborne SAR data.
The first examples of palladium-catalyzed allylic C–H oxidative allylation of sulfoxonium ylides to afford the corresponding conjugated dienones with moderate to good yields have been established. ...The features of this novel conversion include mild reaction conditions, wide substrate scope, and excellent regioselectivity.
•Metabolite changes in fish sauce during fermentation were studied by UPLC-Q/TOF-MS.•Forty-six metabolites were identified as key chemical components of fish sauce.•Aspartic acid and glutamic acid ...play a part in the umami taste of fish sauce.•Specific metabolites were primarily associated with amino acid metabolism.
Spontaneous fermentation is a critical step in the processing of high-quality fish sauce. In this study, a comparative UHPLC-Q/TOF-MS-based metabolomics approach combining equivalent-quantification and the taste activity value (TAV) was used, for the first time, to evaluate the taste qualities and characterize metabolite profiles in Chinese fish sauce during fermentation. A total of 22,816 metabolite ion features were extracted from fish sauce samples. Forty-six metabolites, including amino acids, small peptides, organic acids, amines, carbohydrates, and nucleic acids, were identified as key chemical components of fish sauce. In addition, absolute quantification and TAV showed that aspartic acid and glutamic acid exert an important influence on the umami taste of fish sauce. Specific metabolites were primarily associated with amino acid metabolism, particularly alterations in arginine and proline metabolism. This study identifies chemical components and provides novel insights into the taste quality of fish sauce due to fermentation.
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•A salt-tolerant T. muriaticus is first isolated from traditional Chinese fish sauce.•Correlation network maps reveal microbial metabolism for volatile flavor formation.•T. muriaticus ...reduces the spoilage microorganism abundance in low-salt fish sauce.•T. muriaticus improves volatile flavor types and contents in low-salt fish sauce.•Tetragenococcus contributes most to volatile flavor formation and improvement.
Application of low-salt fish sauce is restricted by its easy spoilage and poor flavor formation. In this study, a salt-tolerant strain Tetragenococcus muriaticus with powerful salt tolerance was isolated as starter for the low-salt fish sauce fermentation. The 16S rRNA gene high-throughput sequencing showed that Staphylococcus was the main microbial genus at the beginning of fermentation, reaching 73.75%, followed by Tetragenococcus (16.36%) and Pseudomonas (6.68%), while Tetragenococcus quickly took over the dominant position with the relative abundance over 56.9% after 10 d fermentation and peaked at the end of fermentation. There were a total of 144 volatile compounds identified by HS-SPME-GC-MS, among which aldehydes, esters, alcohols, heterocycles, ketones, and acids were the main volatile flavor compounds and were mainly produced by Tetragenococcus, Synechococcus, Rhodococcus, Stenotrophomonas, Achromobacter, and Brucella based on the correlation network maps constructed by the Pearson’s correlation coefficient after O2PLS evaluation. The Sobs, ACE, Chao, and Shannon indexes of microbial community were significantly reduced, while the Simpson index was markedly enhanced in the low-salt fish sauce fermented with T. muriaticus compared with them in the fish sauce without starter addition. The addition of T. muriaticus also obviously improved the types and concentrations of main volatile flavor compounds in the low-salt fish sauce. The correlation network map showed that Tetragenococcus was the only genus that played a crucial role in the spoilage microorganism inhibition and volatile flavor improvement in the fish sauce. T. muriaticus can be developed as a potential microbial starter for the industrial production of low-salt fish sauce.
Purpose
Building supply chain (SC) resilience is crucial for business continuity given the ever-changing environmental conditions. Based on the resource orchestration and organizational culture ...theories, the purpose of this paper is to investigate the business value of SC resilience with the consideration of the roles of internal integration (II) and external integration (EI), risk management culture (RMC) and SC flexibility (SCF).
Design/methodology/approach
This study investigates how RMC, SCF and intra and interorganizational integration affect the performance of SC resilience. It collects primary and secondary data from 194 manufacturing firms listed in the Taiwan Stock Exchange and Taipei Exchange.
Findings
Results validate the authors’ hypothesis that RMC, SCF and II improve the financial performance of firms through SC resilience efforts.
Research limitations/implications
This study uses firms from Taiwan manufacturing industry, which might introduce country and industry bias.
Practical implications
This study helps managers improve the financial performance of their SC resilience efforts by developing RMC, SCF, II and IE across functions and partner firms.
Originality/value
This study contributes to the literature by empirically testing the relationship between SC resilience and financial performance, and how the relationship is moderated by RMC, SCF, II and EI based on the theories of organizational culture and resource orchestration.
Brain network provides an essential perspective for studying normal and pathological brain activities. Reconstructing the brain network in the source space becomes more needed, for example, as a ...target in non-invasive neuromodulation. Precise estimating source activities from the scalp EEG is still challenging because it is an ill-posed question and because of the volume conduction effect. There is no consensus on how to reconstruct the EEG source network. This study uses simultaneous scalp EEG and stereo-EEG to investigate the effect of inverse solutions, connectivity measures, and node sizes on the reconstruction of the source network. We evaluated the performance of different methods on both source activity and network. Numerical simulation was also carried out for comparison. The weighted phase-lag index (wPLI) method achieved significantly better performance on the reconstructed networks in source space than five other connectivity measures (directed transfer function (DTF), partial directed coherence (PDC), efficient effective connectivity (EEC), Pearson correlation coefficient (PCC), and amplitude envelope correlation (AEC)). There is no significant difference between the inverse solutions (standardized low-resolution brain electromagnetic tomography (sLORETA), weighted minimum norm estimate (wMNE), and linearly constrained minimum variance (LCMV) beamforming) on the reconstructed source networks. The source network based on signal phases can fit intracranial activities better than signal waveform properties or causality. Our study provides a basis for reconstructing source space networks from scalp EEG, especially for future neuromodulation research.
Neuromodulation has emerged as a promising technique for the treatment of epilepsy. The target for neuromodulation is critical for the effectiveness of seizure control. About 30% of patients with ...drug-resistant epilepsy (DRE) fail to achieve seizure freedom after surgical intervention. It is difficult to find effective brain targets for neuromodulation in these patients because brain regions are damaged during surgery. In this study, we propose a novel approach for localizing neuromodulatory targets, which uses intracranial EEG and multi-unit computational models to simulate the dynamic behavior of epileptic networks through external stimulation. First, we validate our method on a multivariate autoregressive model and compare nine different methods of constructing brain networks. Our results show that the directed transfer function with surrogate analysis achieves the best performance. Intracranial EEGs of 11 DRE patients are further analyzed. These patients all underwent surgery. In three seizure-free patients, the localized targets are concordant with the resected regions. For the eight patients without seizure-free outcome, the localized targets in three of them are outside the resected regions. Finally, we provide candidate targets for neuromodulation in these patients without seizure-free outcome based on virtual resected epileptic network. We demonstrate the ability of our approach to locate optimal targets for neuromodulation. We hope that our approach can provide a new tool for localizing patient-specific targets for neuromodulation therapy in DRE.
Upregulation of CD137 (4-1BB) on recently activated CD8(+) T cells has been used to identify rare viral or tumor antigen-specific T cells from peripheral blood. Here, we evaluated the immunobiology ...of CD137 in human cancer and the utility of a CD137-positive separation methodology for the identification and enrichment of fresh tumor-reactive tumor-infiltrating lymphocytes (TIL) or tumor-associated lymphocytes (TAL) from ascites for use in adoptive immunotherapy.
TILs from resected ovarian cancer or melanoma were measured for surface CD137 expression directly or after overnight incubation in the presence of tumor cells and homeostatic cytokines. CD137(pos) TILs were sorted and evaluated for antitumor activity in vitro and in vivo.
Fresh ovarian TILs and TALs naturally expressed higher levels of CD137 than circulating T cells. An HLA-dependent increase in CD137 expression was observed following incubation of fresh enzyme-digested tumor or ascites in IL-7 and IL-15 cytokines, but not IL-2. Enriched CD137(pos) TILs, but not PD-1(pos) or PD-1(neg) CD137(neg) cells, possessed autologous tumor reactivity in vitro and in vivo. In melanoma studies, all MART-1-specific CD8(+) TILs upregulated CD137 expression after incubation with HLA-matched, MART-expressing cancer cells and antigen-specific effector function was restricted to the CD137(pos) subset in vitro. CD137(pos) TILs also mediated superior antitumor effects in vivo, compared with CD137(neg) TILs.
Our findings reveal a role for the TNFR-family member CD137 in the immunobiology of human cancer where it is preferentially expressed on tumor-reactive subset of TILs, thus rationalizing its agonistic engagement in vivo and its use in TIL selection for adoptive immunotherapy trials.
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•Acid stress induced obvious cross-protection for Cd tolerance in P. kudriavzevii.•Improved Cd conjugation and transport by acid stress reduced Cd toxicity.•Acid stress improved ...antioxidative ability to alleviate Cd-induced oxidative stress.•Heat shock protein HSP12 contributed to the Cd detoxification in P. kudriavzevii.•Cd efflux by YOR1 resulted in the decrease of intracellular Cd concentration.
The toxicity of cadmium (Cd) is the major limitation to its removal using microorganisms. The Cd tolerance of Pichia kudriavzevii was obviously enhanced by acid stress based on multi-stress cross-protection. RNA-Seq showed that most differentially expressed genes (DEGs) in the Pentose phosphate pathway, Citrate cycle (TCA cycle), Glycolysis/Gluconeogenesis, Peroxisome and Glutathione metabolism were up-regulated by acid stress. The up-regulated expression of genes related to ATP synthesis (GOR1, ALD5, ADH4, ADH6, MDH2, IDH1, IDH2, and ATP19) and Cd transport (GSTY2, GTO2, GLO2, and YOR1), and the improvement of intracellular GSH level and GST activity, reduced the Cd toxicity towards P. kudriavzevii. Cd efflux by YOR1 played a key role in the decline of intracellular Cd level. Acid stress obviously improved the gene expression levels and activities of antioxidant enzymes (SOD, POD, and CAT), which inhibited the Cd-induced ROS outburst and oxidative damage of proteins and membrane lipids. In addition, the enhanced expression of HSP12 protected P. kudriavzevii from the damage of Cd stress. These results provide some important clues to reconstruct robust strains using for Cd removal in aquatic environments.
In the surveillance of interested regions by unmanned aerial vehicle (UAV), system performance relies greatly on the motion control strategy of the UAV and the operation characteristics of the ...onboard sensors. This paper investigates the 2D path planning problem for the lightweight UAV synthetic aperture radar (SAR) system in an environment of multiple regions of interest (ROIs), the sizes of which are comparable to the radar swath width. Taking into account the special requirements of the SAR system on the motion of the platform, we model path planning for UAV SAR as a constrained multiobjective optimization problem (MOP). Based on the fact that the UAV route can be designed in the map image, an image-based path planner is proposed in this paper. First, the neighboring ROIs are merged by the morphological operation. Then, the parts of routes for data collection of the ROIs can be located according to the geometric features of the ROIs and the observation geometry of UAV SAR. Lastly, the route segments for ROIs surveillance are connected by a path planning algorithm named the sampling-based sparse A* search (SSAS) algorithm. Simulation experiments in real scenarios demonstrate that the proposed sensor-oriented path planner can improve the reconnaissance performance of lightweight UAV SAR greatly compared with the conventional zigzag path planner.
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