Abstract
A multi-wavelength analysis of the large Galactic infrared bubble N 24 is presented in this paper in order to investigate the molecular and star-formation environment around expanding H ii ...regions. Using archival data from Herschel and ATLASGAL, the distribution and physical properties of the dust over the entire bubble are studied. Using the Clumpfind2d algorithm, 23 dense clumps are identified, with sizes and masses in the range 0.65–1.73 pc and 600–16 300 M⊙, respectively. To analyse the molecular environment in N 24, observations of NH3 (1,1) and (2,2) were carried out using the Nanshan 26-m radio telescope. Analysis of the kinetic temperature and gravitational stability of these clumps suggests gravitational collapse in several of them. The mass–size distributions of the clumps and the presence of massive young protostars indicate that the shell of N 24 is a region of ongoing massive-star formation. The compatibility of the dynamical and fragmentation timescales and the overabundance of young stellar objects and clumps on the rim suggest that the ‘collect-and-collapse’ mechanism is in play at the boundary of the bubble, but the existence of the infrared dark cloud at the edge of bubble indicates that a ‘radiation-driven implosion’ mechanism may also have played a role there.
The kinematic analysis of a single spider universal joint is carried out,by using mathematical vector method,the transfer movement relationship of a single spider universal joint and the cross axis ...plane normal trajectory equation are obtained. By using the Solid Works Motion software,the kinematic trajectory of a single spider universal joint cross axis is simulated,comparing the simulated wave chart with the calculated theoretical equation,the correctness of the theoretical analysis results is verified,a theoretical basis for the single spider universal joint movement optimized design is provided. At the same time in space,a theoretical reference for the spatial arrangement of single spider universal joint steering system is provided.
CD200-CD200R plays a critical role in regulating the human tumor microenvironment, but its role in cervical cancer remains unclear.
A total of 62 paraffin blocks of tumor tissues were collected from ...cervical cancer patients. Expression of CD200 and cathepsin K (CTSK) in cancer tissues and para-cancerous tissues was analyzed by immunohistochemistry. Stably transfected CD200 cells were established in HeLa and SiHa cells. Human THP-1 monocytes were induced to differentiate into M2 macrophages. HeLa and SiHa cells were cultured in conditioned medium from M2 macrophages to observe the effects of CD200-CD200R on invasion, CTSK, p65NF-κB, and cisplatin or paclitaxel sensitivity in cervical cancer cells. HeLa cells were injected to induce xenograft tumors in mice, and a CTSK inhibitor, MK-0822, was used to confirm the regulation of CTSK and paclitaxel sensitivity by CD200-CD200R in vivo.
A significant decrease in CD200 and CTSK expression was found in tumor cancer tissues compared with para-cancerous tissues. Only CD200 overexpression did not affect cervical cell invasion, but CD200-CD200R could enhance the cell invasion and resistance to cisplatin or paclitaxel. Meanwhile, expression of CTSK and p-p65NF-κB in cancer cells stably transfected with CD200 was obviously increased after culture in conditioned medium from M2 macrophages compared with transfection with the plasmid control. In vivo, CTSK inhibition significantly suppressed the effects of CD200-CD200R overexpression on the response to paclitaxel by suppressing the CTSK-mediated NF-κB pathway.
CD200-CD200R regulates CTSK-mediated NF-κB pathway to affect cisplatin or paclitaxel sensitivity in cervical cancer, which provides a possible immunotherapeutic target and combination strategy for advanced cervical cancer.
To explore the potential function of tricin in diabetic retinopathy (DR) and investigate whether Sestrin2 is closely involved in DR. A single intraperitoneal injection of streptozotocin-induced ...diabetes model in Sprague-Dawley rats and a high glucose-induced retinal epithelial cell model in ARPE-19 cells were established. The retinas were removed and examined by hematoxylin-eosin (HE) staining and dihydroethidium (DHE) staining. The proliferation ability and reactive oxygen species (ROS) level of ARPE-19 cells were detected by 5-ethynyl-2′-deoxyuridine (EdU) and flow cytometry. Then, the content of superoxide dismutase (SOD), malonaldehyde (MDA), and glutathione peroxidase (GSH-Px) in serum or cell supernatant was tested using enzyme linked immunosorbent assay (ELISA). In addition, the expression of Sestrin2, nuclear factor erythroid-2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), platelet endothelial cell adhesion molecule-1 (CD31), and vascular endothelial growth factor receptor 2 (VEGFR2) in retina tissue or ARPE-19 cells were validated through western blot and immunofluorescence assays. With the increase of MDA and ROS concentration, Sestrin2 expression was downregulated significantly, and Nrf2 and HO-1 expression was also reduced in retina tissue or ARPE-19 cells of model group, whereas CD31 and VEGFR2 expression was upregulated. However, tricin ameliorated the oxidative stress and angiogenesis and rectified the abnormal expression of Sestrin2/Nrf2 in diabetic retinopathy. Further mechanistic studies showed that silence Sestrin2 reduced the protective effect of tricin on ARPE-19 cells, as well as abolished its regulating effect on the Nrf2 pathway. These results suggested that tricin inhibits oxidative stress and angiogenesis in retinal epithelial cells of DR rats via reinforcing Sestrin2/Nrf2 signaling.
In this study, two innovative surface severe plastic deformation (SSPD) methods, namely abrasive waterjet peening (AWJP) and ultrasonic nanocrystal surface modification (UNSM), were applied to a 304 ...stainless steel to improve the mechanical behavior. The surface roughness, microstructure, residual stress, hardness, and tensile mechanical properties of the alloy after the two SSPD treatments were studied systematically. The results show that both the AWJP and UNSM treatments have greatly positive effects on the mechanical-properties improvements by successfully introducing a hardening layer. Especially the UNSM-processed specimen possesses the most outstanding comprehensive mechanical properties (high strength with the comparable ductility). The yield strength with the UNSM treatment is 443 MPa, corresponding to the 109% and 19% improvements, as compared to that of the base (212 MPa) and AWJP-treated specimens (372 MPa). The results can be attributed to a much thicker hardening layer (about 500 μm) and a better surface integrity with lower roughness (Ra: 0.10 μm) formed by the UNSM technique.
Background Luteolin, a flavonoid compound with anti-inflammatory activity, has been reported to alleviate cerebral ischemia/reperfusion (I/R) injury. However, its potential mechanism remains unclear. ...Methods The binding activity of luteolin to peroxisome proliferator-activated receptor gamma (PPARgamma) was calculated via molecular docking analysis. Rats were subjected to middle cerebral artery occlusion and reperfusion (MCAO/R). After reperfusion, vehicle, 25 mg/kg/d luteolin, 50 mg/kg/d luteolin, 10 mg/kg/d pioglitazone, 50 mg/kg/d luteolin combined with 10 mg/kg/d T0070907 (PPARgamma inhibitor) were immediately orally treatment for 7 days. ELISA, TTC staining, H&E staining, immunohistochemistry, immunofluorescence and transmission electron microscope methods were performed to evaluate the inflammation and autophagy in damaged hippocampal region. The PPARgamma, light chain 3 (LC3) B-II/LC3B-I and p-nuclear factor-κB (NF-κB) p65 proteins expression levels in damaged hippocampal region were analyzed. Results Luteolin showed good PPARgamma activity according to docking score (score = - 8.2). Luteolin treatment downregulated the infarct area and the pro-inflammatory cytokines levels caused by MCAO/R injury. Moreover, luteolin administration ameliorated neuroinflammation and autophagy in damaged hippocampal region. Pioglitazone plays protective roles similar to luteolin. T0070907 concealed the neuroprotective roles of 50 mg/kg/d luteolin. Conclusions Luteolin exerts neuroprotective roles against inflammation and autophagy of hippocampus induced by cerebral I/R by activating PPARgamma in rats. Keywords: Cerebral ischemia-reperfusion, Pioglitazone, T0070907, Molecular docking analysis
The Eocene was a typical greenhouse climate period in the history of the Earth, with a high global sea level and extensive carbonate deposits developed in Tethys. During the Eocene, a carbonate ...platform was deposited in the western Tarim Basin, which belongs to the easternmost Tethys. However, the details of this carbonate platform and its complications for paleoecology and paleoclimate are still unclear. This research focuses on the bivalve-bearing carbonates of the Kalataer Formation in the western Tarim Basin, and detailed analyses of microfacies, biostratigraphy, paleoecology, and sea-level change were carried out. The bivalves of the Kalataer Formation are dominated by the Ostrea ( Turkostrea ) strictiplicata , Ostrea ( Turkostrea ) cizancourti, Ostrea ( Turkostrea ) strictiplicata , Ostrea ( Turkostrea ) strictiplicata, and Sokolowia buhsii , and the age is middle Lutetian of the Eocene. The biota of the Kalataer Formation mainly lives in open shallow sea environments, with medium to low energy and rich nutrients and oxygen, representing a typical shallow marine ecosystem of the carbonate platform. Microfacies and paleoecology indicate a large marine transgression event occurred in the western Tarim Basin during the middle Eocene.
In this study, waterjet peening (WJP) treatments under different water pressures were utilized to improve the mechanical properties of 304 stainless steel. The surface morphologies, microstructures, ...phases, and mechanical properties under different pressures in the WJP process were systematically investigated. The results show that WJP treatments successfully introduced a hardening layer and residual compressive stress. The optimal hardening layer, hardness, residual compressive stress, tensile strength, and ductility were all recorded at the pressure of 200 MPa. The improved hardness, tensile strength, and ductility of 304 stainless steel treated with WJP treatments at the pressure of 200 MPa can be attributed to the hardening layer with much apparent grain refinement effect, phase transformation, smaller surface roughness, and a specific residual compressive stress, as compared with the WJP treatments under other water pressures.
The water jet peening (WJP) technology can induce compressive residual stress (RS) in metal surfaces and, thus, improve the fatigue life of components. In this paper, a mathematical model is proposed ...for calculating the RS induced by WJP. To validate the proposed mathematical model, experimental and finite element simulation verifications were carried out on Al6061-T6. The distribution of RS along the depth direction, the maximum compressive RS, and the depth of the compressive RS layer were also investigated based on the mathematical model. Results showed that the error of maximum compressive RS between the mathematical model and experiment was within 9% under a jet pressure of 60 MPa, and the error of depth of the compressive RS layer between the mathematical model and experiment was within 13% under a jet diameter of 0.3 mm. Hence, the mathematical model is reliable and accurate. The maximum compressive RS increases with the increase in jet pressure, and the depth of the compressive RS layer approximately linearly increases with the increase in jet diameter.
Infrared (IR) small target detection in complex backgrounds is one of the key technologies in IR search and tracking applications. Although significant progress has been made over the past few ...decades, how to separate a small target from complex backgrounds remains a challenging task. In this letter, a novel small target detection method via multidirectional derivative-based weighted contrast measure (MDWCM) is proposed. Initially, multidirectional derivative subbands are quickly obtained by the facet model. Then, an effective division scheme of surrounding area is performed to capture the derivative properties of the target. A new local contrast measure is constructed to simultaneously enhance the target and suppress the background clutter. Third, the MDWCM maps constructed from all derivative subbands are integrated to enhance the robustness of detection. Finally, the small target is extracted by an adaptive segmentation method. The experimental results demonstrate that the proposed algorithm performs favorably compared to other state-of-the-art methods.
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