Advanced cancer (AC) patients experience serious physical and psychological problems with the disease progression. When approaching the end of life, these patients have to cope with not only the ...bodily illness, but also the spiritual crisis. Conventional psychological treatments reduce distress to a certain extent, but for patients with AC, especially when they face progressive illness and are approaching death, their psychological problems are complex, and no simple solutions are in sight. Therefore, we designed this study to evaluate the efficacy of the combined Naikan therapy (NT) and Morita therapy (MT) on psychological distress and posttraumatic growth in patients with AC.
One hundred thirty patients newly diagnosed with AC were allocated randomly into treatment (n = 65) and control (n = 65) groups. Patients in the treatment group received combined NT and MT for 7 consecutive weeks, while the control group received normal medical treatments without NT and MT. Patients were assessed before and after the therapies. The primary outcome measures include distress thermometer (DT) and posttraumatic growth, and the secondary outcome measure contains the list of distress problems.
At the post-treatment stage, the treatment group displayed a decreased score of psychological distress as compared to that in the control group, which accompanied by a higher post-traumatic growth total score and subscale scores in relationship to others, new possibilities, personal strength, spiritual changes, and appreciation of life. A significant decrease in fear, sleeping difficulty/insomnia, nervousness/anxiety, and loss of appetite was also observed in the treatment group.
The results proved that the combined Naikan and Morita therapies decreased the psychological distress and improved the posttraumatic growth of the patients with AC.
ChiCTR1900026691.
Objective Modifying 3D tubular DNA origami nanostructures (DONs) onto the mesenchymal stem cells (MSCs) membrane surface to obtain DONs@MSCs. Utilizing the characteristics of MSCs homing lung tissue ...to achieve targeted delivery of DONs to the lungs of mice. Methods The computer-aided tools for DNA sequence were used to design and obtain obtain pre-designed DONs. The morphology of assembled DONs was characterized by atomic force microscopy(AFM), and the upload of functional groups was verified by agarose gel electrophoresis (AGE). Fluorescent labeled single-strand DNA(ssDNA) was modified on the surface of MSCs using biological orthometabolic sugar engineering. According to the principle of complementary base pairing, DONs were modified on the MSCs surface. Using confocal microscopy and flow cytometry to detect the ssDNA and DONs, and using in vivo imaging to observe the distribution of DONs in lung tissue. Results DONs had been successfully assembled. MSCs membrane surface was successfully modified with ssDNA,
Background Gefitinib is widely used in the treatment of advanced non-small cell lung cancer （NSCLC）.However,only a small number of reports have described initial failure sites in patients treated ...with gefitinib.The aim of this study was to investigate survival,recurrence sites,and treatment after recurrence in these patients.Methods A retrospective review was conducted of all patients with stage Ⅲ/Ⅳ NSCLC treated with gefitinib in Peking Union Medical College Hospital from October 2002 to September 2011.Patient characteristics,initial failure sites,associated clinical factors,and subsequent therapy were included in the analysis of prognostic factors.Results A total of 316 patients were identified The median progress free survival （PFS） and overall survival （OS） times were 238 days and 468 days,respectively.The median survival time after progression was 145 days.The sites of initial failure were lung （62.34%）,bone （17.72%）,central nerve system （CNS,16.14%）,liver （9.49%）,and others （7.19%）.Patients with single-site progression or multi-site progression were 81.01% and 18.99%,respectively.Progression-free survival time was associated with lung and bone failure.Additionally,the median survival time after progression was lower in patients with multi-site progression and liver progression.Other initial failure sites displayed no relationship with survival,including CNS failure.Subsequent therapy may affect survival after progression.In patients receiving continuous epidermal growth factor receptor tyrosine kinase inhibitor （EGFR-TKI） therapy,chemotherapy,radiotherapy,and retreatment with EGFR-TKIs,survival time after progression was prolonged compared with the best supportive care.Conclusions Our data suggest that patients receiving gefltinib should be closely monitored regarding lung metastasis during follow-up.Liver metastases and multi-site progression were poor prognostic factors.After failure with gefltinib,patients may benefit from radiotherapy,chemotherapy,continuous EGFR-TKI therapy and re-treatment with EGFR-TKIs.
Chemosensory proteins (CSPs) are small soluble proteins often associated with chemosensory organs in insects but include members involved in other functions, such as pheromone delivery and ...development. Although the CSPs of the sensory organs have been extensively studied, little is known on their functions in other parts of the body. A first screening of the available databases has identified 70 sequences encoding CSPs in the oriental locust
. Applying proteomic analysis, we have identified 17 of them abundantly expressed in the female reproductive organs, but only one (CSP91) in male organs. Bacterially expressed CSP91 binds fatty acids with a specificity for oleic and linoleic acid, as well as medium-length alcohols and esters. The same acids have been detected as the main gas chromatographic peaks in the dichloromethane extracts of reproductive organs of both sexes. The abundance and the number of CSPs in female reproductive organs indicates important roles for these proteins. We cannot exclude that different functions can be associated with each of the 17 CSPs, including delivery of semiochemicals, solubilization of hormones, direct control of development, or other unknown tasks.
To compare the effects of 4 weeks of intervention using conventional rehabilitation, intensive conventional rehabilitation and modified constraint-induced movement therapy on the hemiplegic upper ...extremity in stroke patients.
Thirty stroke patients (mean age: 63.3, standard deviation 9.63 years; mean time since stroke: 11.33, standard deviation 8.29 weeks) were randomly divided into 3 groups: conventional rehabilitation, intensive conventional rehabilitation, and modified constraint-induced movement therapy (10 individuals in each). Motor function was assessed using the Wolf Motor Function Test before treatment, and 2 weeks and 4 weeks after treatment.
The constraint-induced movement therapy and intensive conventional rehabilitation groups improved their function ability scores in the Wolf Motor Function Test significantly more than the conventional rehabilitation group after 2 weeks of treatment (p < 0.05), but all groups reached comparable levels at the end of 4 weeks of intervention. However, only the constraint-induced movement therapy intervention proved to have robust and systematic effects on the function ability scores, as revealed by the large, positive and significant correlation between the initial scores and the scores 2 and 4 weeks after the intervention. The median performance time of the Wolf Motor Function Test decreased significantly in all groups after 4 weeks of treatment (p < 0.05), but only the modified constraint-induced movement therapy group showed significant improvements both 2 and 4 weeks after the initiation of treatment.
Compared with classical intervention, modified constraint-induced movement therapy showed an apparent advantage over both conventional intervention and intensive conventional rehabilitation for patients after stroke.
Objective To investigate the effects of exosome secreted by macrophage J774A.1 on the proliferation and migration of mouse colorectal cancer cell lines MC38 and CT26 and potential mechanism. Methods ...The phenotype of J774A.1 cells was identified by flow cytometry.The morphological characteristics, size and surface markers of the exosome of J774A.1 cells were detected by transmission electronic microscopy, nanoparticle tracking analysis(NTA) and Western blot.The uptake of exosome by MC38 and CT26 cells was observed by fluorescence microscopy. Proliferation of MC38 and CT26 was detected by CCK8 assay. Migration ability of MC38 and CT26 was detected by wound healing assay. The expression of phosphorylated proteins p-MEK1/2 and p-ERK1/2 in proliferation-related pathways was detected by Western blot. Results J774A.1 cells are M2 macrophages expressing the cell surface marker molecules CD68 and CD206. J774A.1 exosomes can be taken up by colorectal cancer cells MC38 and CT26, and can promote their proliferation(P＜0.0
Background and Aim: Overexpression of the human epidermal growth factor receptor 2 (HER‐2) protein has been detected in gastric cancer and has been associated with an unfavorable prognosis. We ...investigated the anti‐cancer effects of anti‐p185HER‐2 ricin A chain (RTA) immunotoxin, alone or in combination with 5‐flurouracil on SGC7901‐HER‐2+ cells.
Methods: SGC7901‐HER‐2+ cells were obtained by transfecting SGC7901 cells with HER‐2‐pcDNA3.1. Anti‐p185HER‐2‐RTA was prepared by chemical conjugation of anti‐HER‐2 monoclonal antibody (mAb) and RTA. The SGC7901‐HER‐2+ cells were incubated with RTA, anti‐p185HER‐2‐RTA, and/or 5‐flurouracil. The effects of drugs on cells were evaluated by MTT assay and Annexin V‐fluorescein isothiocyanate and propidium iodide double staining flow cytometry. The expression of caspase‐3, caspase‐9, cyclooxygenase‐2, and nuclear factor‐κB/p65 were assayed by western blot. SGC7901‐HER‐2+ cells were transplanted into BALB/c nude mice to produce solid tumors in an attempt to study the immunotoxin activity in vivo.
Results: In vitro, anti‐p185HER‐2‐RTA inhibited cell growth and induced apoptosis in SGC7901‐HER‐2+ cells. Anti‐p185HER‐2‐RTA enhanced caspase‐3 and caspase‐9 activity, while downregulating the expression of cyclooxygenase‐2 and nuclear factor‐κB/p65. Its combination with 5‐flurouracil further inhibited the growth of SGC7901‐HER‐2+ cells. In vivo, our data showed that anti‐p185HER‐2‐RTA significantly inhibited the growth of SGC7901‐HER‐2+ cells‐transplanted tumors.
Conclusions: Anti‐p185HER‐2‐RTA inhibits the growth of SGC7901‐HER‐2+ cells. The effect may be related to the activation of caspase‐3 and caspase‐9 and inhibition of cyclooxygenase‐2 and nuclear factor‐κB/p65. Anti‐p185HER‐2‐RTA plus 5‐FU enhance anti‐cancer activity, suggesting useful clues for further study for the treatment of HER‐2 positive gastric cancers.
Considering the instability and low photoluminescence quantum yield (PLQY) of blue‐emitting perovskites, it is still challenging and attractive to construct single crystalline hybrid lead halides ...with highly stable and efficient blue light emission. Herein, by rationally introducing d10 transition metal into single lead halide as new structural building unit and optical emitting center, we prepared a bimetallic halide of (NH4)2CuPbBr5 with new type of three‐dimensional (3D) anionic framework. (NH4)2CuPbBr5 exhibits strong band‐edge blue emission (441 nm) with a high PLQY of 32 % upon excitation with UV light. Detailed photophysical studies indicate (NH4)2CuPbBr5 also displays broadband red light emissions derived from self‐trapped states. Furthermore, the 3D framework features high structural and optical stabilities at extreme environments during at least three years. To our best knowledge, this work represents the first 3D non‐perovskite bimetallic halide with highly efficient and stable blue light emission.
It turns blue! The first crystalline three‐dimensional bimetallic halide of (NH4)2CuPbBr5 exhibits strong narrow blue emission with high PLQY of 32 % as well as remarkable structural and optical stabilities. This structural design strategy is entirely different from the modification methods over CsPbX3 quantum dots and paves a unique way to realize blue light emission based on single‐crystalline hybrid metal halides.
Arthrogryposis,renal dysfunction and cholestasis(ARC)syndrome is a rare genetic disorder and has not been described in China.We present a female infant with neonatal intrahepatic cholestasis from a ...Chinese family with ARC syndrome.All 23 coding exons and flanking introns of the VPS33B gene were amplified and sequenced using peripheral lymphocyte genomic DNA of the patient and her parents.Genetic testing revealed two novel mutations(c.1033delA and c.1567C>T)in the VPS33B gene.The patient is a compound heterozygote and her parents were heterozygous for each of the mutations.
Lung cancer is the leading cause of cancer death in the world. Schizandrin B (Sch B) is one of the main dibenzocyclooctadiene lignans present in the fruit of Schisandra chinensis (Schisandraceae). ...Sch B has multiple functions against cancer. The aim of this study was to determine the effect of Sch B on the proliferation, cell cycling, apoptosis and invasion of lung adenocarcinoma A549 cells by MTT, flow cytometry, wound healing and transwell invasion assays. Treatment with Sch B inhibited the proliferation of A549 cells in a dose-dependent manner. Sch B induced cell cycle arrest at G0/G1 phase by down-regulating the expression of cyclin D1, cyclin-dependent kinase (CDK)4, and CDK6, but up-regulating p53 and p21 expression in A549 cells. Furthermore, Sch B triggered A549 cell apoptosis by increasing Bax, cleaved caspase-3, 9, Cyto C, but decreasing Bcl-2 and PCNA expression. In addition, Sch B inhibited the invasion and migration of A549 cells by down-regulating the expressions of HIF-1, VEGF, MMP-9 and MMP-2. Therefore, Sch B has potent anti-tumor activity and may be a promising traditional Chinese medicine for human lung carcinoma.
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