piRNA‐823 as a member of the piRNA family is reported to promote tumour cell proliferation in multiple myeloma and hepatocellular cancer. However, few studies on the function of piRNA‐823 in ...colorectal cancer (CRC). Our present study data showed that piRNA‐823 plays an oncogene role in CRC cells. Inhibition of piRNA‐823 can significantly inhibit the proliferation, invasion and apoptosis resistance of CRC cells. Mechanism studies have shown that piRNA‐823 inhibits the ubiquitination of hypoxia‐inducible factor‐1 alpha (HIF‐1α) by up‐regulating the expression of Glucose‐6‐phosphate dehydrogenase (G6PD) and ultimately up‐regulates the glucose consumption of carcinoma cells and inhibits the content of intracellular reactive oxygen species (ROS). Therefore, we speculate piRNA‐823 promotes the proliferation, invasion and apoptosis resistance of CRC cells by regulating G6PD/HIF‐1α pathway. In this study, we set up the cancer‐promoting function recovery experiment of piRNA‐823 by silencing G6PD gene to confirm the dominance of the above‐mentioned pathways. Using clinical samples, we found that overexpression of piRNA‐823 correlated with poor overall survival and predicted a poor response to adjuvant chemotherapy of patients with CRC. In a word, our research has further enriched the theory of piRNA‐823 promoting the progression of CRC, and laid a solid foundation for the development of piRNA‐823‐based gene therapy for CRC and its use as a promising prognostic biomarker in CRC patients.
The field boundaries in our country are complex. In attempts to control pests via trichogramma-dominated biological control, the long-term practice of manual trichogramma release has resulted in low ...control efficiency, thereby impeding sustainable agricultural development. Currently, the novel approach involves utilizing Unmanned Aerial Vehicles (UAVs) for trichogramma balls delivery; however, the system is still in its nascent stages, presenting opportunities for enhancement in terms of stability and accuracy. Furthermore, there is a notable absence of comprehensive operational quality assessment standards. In this study, we establish a stable and accurate trichogramma balls delivery system using a four-axis plant protection UAV and introduce a comprehensive evaluation method for trichogramma balls delivery system. When dealing with fields with complex boundaries, it is beneficial to divide them into rectangular, trapezoidal, and stepped small fields at the boundary and perform operations within these small fields. According to our proposed evaluation method, when only considering the effect of field operations, the most effective boundary division shape is trapezoidal, followed by rectangular. and the worst is stepped. If both field operation effectiveness and the utilization effect of placed trichogramma balls are considered, the optimal shape is trapezoidal, then stepped, with rectangular being the least effective. Consequently, for UAV sub-area operations in complex boundary fields, it is advisable to divide the boundaries into trapezoids wherever possible. Field experiment results indicate that the system's delivery area can reach up to 4158 m²/min and the coverage rate of released trichogramma balls can exceed 97%. The system design methodology and comprehensive operational quality evaluation method proposed in this article provide technical support and scientific basis for the application and promotion of UAV delivery trichogramma balls system. This is conducive to the high-quality development of agriculture.
Mast cells (MCs) are tissue-resident effector cells that could be the earliest responder to release a unique, stimulus-specific set of mediators in hepatic ischemia–reperfusion (IR) injury However, ...how MCs function in the hepatic IR has remained a formidable challenge due to the substantial redundancy and functional diverse of these mediators. Tryptase is the main protease for degranulation of MCs and its receptor-protease-activated receptor 2 (PAR-2) is widely expressed in endothelial cells. It is unclear whether and how tryptase/PAR-2 axis participates in hepatic IR. We employed an experimental warm 70% liver IR model in mice and found that tryptase was accumulated in the circulation during hepatic IR and positively correlated with liver injury. Tryptase inhibition by protamine can significantly down-regulate the expression of adhesion molecules and reduce neutrophil infiltration within the liver. The level of inflammatory factors and chemokines were also consistent with the pathological change of the liver. In addition, the treatment with exogeneous tryptase in MC-deficient mice can induce the damage observed in wild type mice in the context of liver IR. In vitro, neutrophil infiltration and inflammatory factor secretion were regulated by Tryptase/PAR-2, involving the adhesion molecule expression to regulate neutrophil adhesion dependent on NF-κB pathway. Conclusion: tryptase/PAR-2 participates in liver injury through the activation of LSECs in the early phase of liver IR.
Abstract
Flexible photodetectors (FPDs), which provide excellent advantages such as great wearability, portability, even implantability, have attracted tremendous interest in wearable healthcare ...monitoring, bendable imaging sensors, portable optical communication, etc. Recently, organic and perovskite‐based photoactive materials have been considered promising candidates for FPDs due to their superior optoelectronic performance, appealing mechanical flexibility, low‐temperature solution processability, and cost‐effectiveness. In this review, the milestone progress of organic, perovskite, and organic‐perovskite hybrid‐based FPDs and their applications in artificial intelligence are summarized. First, a brief introduction of device configurations, working principles, and key parameters for FPDs are presented. Subsequently, functional materials in FPDs, especially flexible photoactive materials of organic, perovskite, and organic‐perovskite hybrids, are summarized and analyzed. More importantly, representative applications of FPDs are summarized, including wearable healthcare monitoring, imaging sensors, and optical communication. The outlook and challenges for the field are proposed at the end. The purpose of this review is to not only elaborate on the fundamental design principles of FPDs, but also serve as a roadmap for next‐generation artificial intelligence sensing technologies.
Distributed Optical Fiber Sensing Technology, with advantages such as long detection distance, resistance to electromagnetic interference, and easy maintenance, is widely applied in various fields ...including security monitoring, electrical safety, and aerospace. However, the current Distributed Optical Fiber Sensing Technology faces challenges such as short localization distance, limited range for stress measurement, low accuracy, and time-consuming processes. This study proposes a novel approach for extracting Brillouin frequency shift signals using an adaptive gradient descent algorithm (Adam Algorithm). A Brillouin strain testing system based on heterodyne coherent detection is also constructed. Experimental results show that the distributed strain testing system using the Adam Algorithm can achieve accurate and fast measurement of maximum strain up to 9500 µϵ within a range of 10 kilometers. The average strain measurement deviation is 32.88 µϵ, and the time required for frequency shift extraction is less than 18.5 ms. This method provides a theoretical and experimental basis for the application of BOTDR Distributed Optical Fiber Sensing Technology.
We disclose herein a strategy for the rapid synthesis of versatile organoselenium compounds under mild conditions. In this work, magnesium-based selenium nucleophiles are formed in situ from easily ...available organic halides, magnesium metal, and elemental selenium via mechanical stimulation. This process occurs under liquid-assisted grinding (LAG) conditions, requires no complicated pre-activation procedures, and operates broadly across a diverse range of aryl, heteroaryl, and alkyl substrates. In this work, symmetrical diselenides are efficiently obtained after work-up in the air, while one-pot nucleophilic addition reactions with various electrophiles allow the comprehensive synthesis of unsymmetrical monoselenides with high functional group tolerance. Notably, the method is applied to regioselective selenylation reactions of diiodoarenes and polyaromatic aryl halides that are difficult to operate via solution approaches. Besides selenium, elemental sulfur and tellurium are also competent in this process, which showcases the potential of the methodology for the facile synthesis of organochalcogen compounds.
Copper (Cu) is an essential nutrient for plant growth and development. This metal serves as a constituent element or enzyme cofactor that participates in many biochemical pathways and plays a key ...role in photosynthesis, respiration, ethylene sensing, and antioxidant systems. The physiological significance of Cu uptake and compartmentalization in plants has been underestimated, despite the importance of Cu in cellular metabolic processes. As a micronutrient, Cu has low cellular requirements in plants. However, its bioavailability may be significantly reduced in alkaline or organic matter-rich soils. Cu deficiency is a severe and widespread nutritional disorder that affects plants. In contrast, excessive levels of available Cu in soil can inhibit plant photosynthesis and induce cellular oxidative stress. This can affect plant productivity and potentially pose serious health risks to humans via bioaccumulation in the food chain. Plants have evolved mechanisms to strictly regulate Cu uptake, transport, and cellular homeostasis during long-term environmental adaptation. This review provides a comprehensive overview of the diverse functions of Cu chelators, chaperones, and transporters involved in Cu homeostasis and their regulatory mechanisms in plant responses to varying Cu availability conditions. Finally, we identified that future research needs to enhance our understanding of the mechanisms regulating Cu deficiency or stress in plants. This will pave the way for improving the Cu utilization efficiency and/or Cu tolerance of crops grown in alkaline or Cu-contaminated soils.
Sunburn is common in pomegranate, and sunburned fruits have poor appearance and low marketability. However, the physiological and metabolic responses to sunburn and their underlying molecular ...mechanisms in pomegranate fruit are little understood. Fruit of sunburn-sensitive cultivar 'Hongyushizi' was used to carry out physiological parameter detection and widely-targeted metabolomics and transcriptome study.
Malondialdehyde and relative conductivity increased with the severity of sunburn, which indicated increased membrane injury. Meanwhile, the content of antioxidants (total phenols and flavonoids), which reduce and repair membrane damage, increased and were accompanied by increases in total antioxidant capacity. In sunburned fruits compared with controls, 129 metabolites changed (including naringenin, pelargonidin and kaempferol) and 447 differentially expressed genes including CHI (Pgr25966.1), F3'5'H (Pgr26644.1), and CHS (Pgr005566.1) may have contributed to these changes. Transcription factors, such as NAC 5 (Pgr008725.1), MYB 93 (Pgr001791.1), and MYB 111 (Pgr027973.1) may be involved in phenylpropanoid and flavonoid biosynthesis by regulating the CHI, F3'5'H, and CHS etc. CONCLUSIONS: These findings provide insight into the sunburn mechanisms of pomegranate, and also into the genetic improvement of fruit sunburn.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
With continuous improvements in people’s consumption levels, consumers’ demands for safe and fresh agricultural products increase. The increase in the number of vehicles and serious congestion on ...roads has led to problems, such as the weak timeliness of urban cold chain logistics, high carbon emissions, low customer value and reduced customer satisfaction. In this study, carbon emissions, customer satisfaction, customer value and cost are considered, and an optimization algorithm is established to solve the time-dependent vehicle routing problem in urban cold chain logistics. For road congestion at different time periods during the cold chain distribution process, the segment function is used to express the vehicle speed. According to the characteristics of the model, considering the constraints of the time window and vehicle capacity, an improved NSGA-II algorithm with the local optimization characteristics of the greedy algorithm (G-NSGA-II) is proposed, and the sorting fitness strategy is optimized. In addition, we carry out a series of experiments on existing vehicle routing problem examples and analyze them in a real background to evaluate and prove the effectiveness of the proposed model and algorithm. The experiment results show that the proposed approach effectively reduces the total cost, enhances customer value and promotes the long-term development of logistics companies.
Vivid haptic feedback remains a challenge in truly immersive virtual reality and augmented reality. As the tactile sensitivity among different individuals and different parts of the hand within a ...person varies widely, a universal method to encode tactile information into faithful feedback in hands according to sensitivity features is urgently needed. In addition, existing haptic interfaces worn on the hand are usually bulky, rigid and tethered by cables, which is a hurdle for accurately and naturally providing haptic feedbacks. Here we report a soft, ultrathin, miniaturized and wireless electrotactile system (WeTac) that delivers current through the hand to induce tactile sensations as the skin-integrated haptic interface. With a relatively high pixel density over the whole hand area, the WeTac can provide tactile stimulation and measure the sensation thresholds of users in a flexible way. By mapping the thresholds for different electrical parameters, personalized threshold data can be acquired to reproduce virtual touching sensations on the hand with optimized stimulation intensity and avoid causing pain. With an accurate control of sensation level, temporal and spatial perception, it allows providing personalized feedback when users interact with virtual objects. This technique is promising for a more vivid touching experience in the virtual world and in human–machine interactions.The haptic interface is an essential part of human–machine interfaces where tactile information is delivered between human and machine. Yao et al. develop a soft, ultrathin, miniaturized and wireless electrotactile system that allows virtual tactile information to be reproduced over the hand.
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