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Physicochemical, texture indexes, microstructure and volatiles were used to characterize the changes in quality, structure and flavor of cooked salted egg yolks (SEYs) with or without ...ultrasonic treatment. Experimental results indicated that ultrasonic significantly increased cooking (water) loss, oil exudation, lipids oxidation (TBARS), accelerated the doneness of cooked SEYs and then promoted the generation of volatiles. These results were further confirmed by the improvement of thermal stability, the changes in color, secondary structure of proteins, water distribution and mobility. Meanwhile, more “fragments” and “cracks” were observed in scanning electron microscope (SEM) and the decrease in gumminess and chewiness were detected using texture profile analysis (TPA), inducing that the migration of lipids and collapse of gel network were intensified. Moreover, ultrasonic treatment decreased the content of sodium chloride in SEYs. Therefore, it was concluded that the doneness, quality and flavor of cooked SEYs were improved by ultrasonic treatment, which could be used as an effective and alternative method for the production of SEYs with good flavor, sandy and oily texture.
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•Small and uniform (su) OVA solution was obtained by ultrasound especially at 300 W.•Emulsification properties of OVA-PES-PSO emulsions were enhanced by suOVA particles.•Interface and ...rheology properties of OVA-PEC-PSO emulsions were promoted by suOVA.•Antioxidant activity of OVA-PEC-PSO emulsion were improved by suOVA particles.•The oxidation of pumpkin seed oil was delayed in suOVA-PEC-PSO emulsion.
Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation. In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion. The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets. The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution. Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed. These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.
Osteosarcoma is the most common primary bone malignancy and is a neoplasm thought to be derived from the bone‐forming mesenchymal stem cells. Aberrant activation of oncogenes and inactivation of ...tumour suppressor genes by somatic mutations and epigenetic mechanisms play a pivotal pathogenic role in osteosarcoma. Aside from alterations in these protein‐coding genes, it has now been realized that dysregulation of non‐coding RNAs (ncRNAs), including microRNAs (miRNAs), long non‐coding RNAs (lncRNAs) and the recently discovered circular RNAs (circRNAs), is crucial to the initiation and progression of osteosarcoma. CircRNAs are single‐stranded RNAs that form covalently closed loops and function as an important regulatory element of the genome through multiple machineries. Recently, an increasing number of studies suggested that circRNAs also played critical roles in osteosarcoma. This review summarizes recent development and progression in circRNA transcriptome analysis and their functions in the modulation of osteosarcoma progression.
The representative diagram of circRNA mediated ceRNA network and oncogene signaling pathways. The current study in osteosarcoma highlights the regulatory relationship of the circRNA‐miRNA‐mRNA network for different signaling pathways. Multiple identified circRNAs function as miRNA sponge and subsequently upregulate relevant target genes expression level.
Effective prediction of gas concentrations and reasonable development of corresponding safety measures have important guiding significance for improving coal mine safety management. In order to ...improve the accuracy of gas concentration prediction and enhance the applicability of the model, this paper proposes a long short-term memory (LSTM) cyclic neural network prediction method based on actual coal mine production monitoring data to select gas concentration time series with larger samples and longer time spans, including model structural design, model training, model prediction, and model optimization to implement the prediction algorithm. By using the minimum objective function as the optimization goal, the Adam optimization algorithm is used to continuously update the weight of the neural network, and the network layer and batch size are tuned to select the optimal one. The number of layers and batch size are used as parameters of the coal mine gas concentration prediction model. Finally, the optimized LSTM prediction model is called to predict the gas concentration in the next time period. The experiment proves the following: The LSTM gas concentration prediction model uses large data volume sample prediction, more accurate than the bidirectional recurrent neural network (BidirectionRNN) model and the gated recurrent unit (GRU) model. The average mean square error of the prediction model can be reduced to 0.003 and the predicted mean square error can be reduced to 0.015, which has higher reliability in gas concentration time series prediction. The prediction error range is 0.0005–0.04, which has better robustness in gas concentration time series prediction. When predicting the trend of gas concentration time series, the gas concentration at the time inflection point can be better predicted and the mean square error at the inflection point can be reduced to 0.014, which has higher applicability in gas concentration time series prediction.
Chicken egg chalaza (CLZ) is a natural colloidal structure in eggs that exists as an egg yolk stabilizer and is similar in composition to egg white. In this study, the proteome, phosphoproteome, and ...N-glycoproteome of CLZ were characterized in depth. We hydrolyzed the CLZ proteins and enriched the phosphopeptides and glycopeptides. We identified 45 phosphoproteins and 80 N-glycoproteins, containing 59 phosphosites and 203 N-glycosylation sites, respectively. Typically, the ovalbumin in CLZ was both phosphorylated and N-glycosylated, with 4 phosphosites and 4 N-glycosylation sites. Moreover, we identified 2 N-glycosylated subunits of ovomucin, mucin-5B and mucin-6, with 32 and nine N- glycosylation sites, respectively. Analysis of the phosphorylation and N-glycosylation status of CLZ proteins could provide novel insights into the structural and functional characteristics of CLZ.
Abstract
Background
It is not clear whether modified facet fusion (MFF) is biomechanically different from traditional fusion techniques such as posterior lateral lumbar fusion (PLF) and posterior ...lumbar interbody fusion (PLIF).
Methods
In this study, a healthy adult Chinese male volunteer was selected to perform 3D reconstruction of CT image data and simulate the successful fusion of L4–5 MFF, PLF and PLIF, respectively. The motion range of L4–5 segments of the model was simulated under 6 working conditions, including forward flexion, extension, lateral flexion and rotation under normal physiological conditions, and the stability of the three fusion procedures in the pathological segments of the lumbar spine was compared.
Results
There was no difference in range of motion between MFF model and PLF or PLIF model (
P
< 0.05). Also, the stiffness of the PLFand the MFF model were comparable (
P
> 0.05), but were smaller than the PLIF model (
P
< 0.05).
Conclusions
MFF provides reliable stability at the lumbar fixation fusion level and does not differ significantly from PLF and PLIF in terms of range of motion.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Cervical cancer is the fourth most common cancer in women worldwide, and arsenic has a certain effect in solid tumor chemotherapy. As the rate-limiting enzyme subunit of GSH synthesis, GCLC may be an ...important target for arsenic to induce apoptosis through mitochondrial apoptosis pathway to exert anti-tumor effect. NF-κB plays an important role in the occurrence and development of cervical cancer and can regulate the expression of GCLC. miR-21 is a potential biomarker of cervical cancer, which can induce apoptosis through ROS regulated the mitochondrial pathway of cells. However, the role of miR-21 in the mitochondrial pathway of cervical cancer cells induced by NaAsO2 through NF-κB/GCLC and GSH synthesis regulated oxidative stress is rarely reported. Therefore, the purpose of this study was to investigate whether NaAsO2 might induce mitochondrial damage and apoptosis of cervical cancer cells through NF-κB/ miR-21 /GCLC induced oxidative stress, and play the anti-tumor role of arsenic as a potential drug for the treatment of cervical cancer.
Hela cells were treated with different concentrations of NaAsO2, D, L-Buthionine-(SR)-sulfoximine (BSO), IκBα inhibitor (BAY 11-7082) and miR-21 Inhibitor. CCK-8 assay, Western Blot, qRT PCR, immunofluorescence, transmission electron microscopy, mitochondrial Membrane Potential Assay Kit with JC-1,2′,7′-Dichlorofluorescin diacetate fluorescent probe and Annexin V-FITC were used to measure cell activity, GSH and ROS, mitochondrial morphology and membrane potential (ΔΨm), protein and mRNA expression of GCLC, GCLM, p65, IκBα, p-P65, p-I κBα, Bcl-2, BAX, Caspase3, cleaved-caspase3 and miR-21.
Compared with the control group, with the gradual increasing dose of NaAsO2, cell viability was considerable reduced, and increased rate of apoptosis, intracellular GSH level was decreased significantly, ROS was increased, mitochondrial structure was damaged, mitochondrial membrane potential ΔΨm and Bcl2/BAX lowered, the expression of Caspase3 and cleaved-caspase3 were significantly increased, resulting in mitochondrial apoptosis. When Hela cells were treated with 15, 20, and 25 μmol/L NaAsO2, the mRNA and protein levels of GCLC and GCLM were reduced, the expression of p65 in the nucleus was increased, the expression of p-p65/p65, p-IκBα/IκBα and miR-21 were significantly increased. When BSO increased the inhibitory effect of NaAsO2 on GCLC, Compared with NaAsO2 group, the ΔΨm and protein of Bcl-2/BAX, caspase3 and cleaved-capsase3 were increased. When BAY 11–7082 combined with NaAsO2 co-treated, compared with the NaAsO2 group, the protein and mRNA expression of GCLC was increased, NaAsO2-increased expression level of miR-21 was suppressed, and the ΔΨm and cell viability were higher. In addition, compared with the combination of NaAsO2 and miR-21NC, the protein expression of GCLC was increased, the ΔΨm and cell viability reduction were alleviated by miR-21 Inhibitor combined with NaAsO2.
NaAsO2 may lead to ROS accumulation in Hela cells and trigger mitochondrial apoptosis. The mechanism may be related to the activation of NF-κB signaling pathway and the promotion of miR-21 expression which leads to the inhibition of GCLC expression and the significant decrease of intracellular reductive GSH synthesis.
The role of NF-κB/miR-21/GCLC in the apoptosis of mitochondrial damage induced by NaAsO2 Display omitted
•NaAsO2 induces mitochondrial pathway apoptosis in Hela cells by inhibiting GSH synthesis.•NaAsO2 can inhibit the synthesis of GSH by inhibiting the expression of GCLC.•The activation of NF-κB/miR-21 plays an important role in the regulation of GCLC by NaAsO2.
Coal–rock combination is an important structure for understanding the mechanism of coal and gas outburst, rock burst and coal roadway deformation. In the study, theoretical analysis and physical ...similarity simulation were adopted to investigate the influencing law and mechanism of coal thickness on the compressive strength and prepare five types of coal–rock combination specimens. Uniaxial compression tests were conducted to obtain the characteristics of the final failure state and the variation law of the compressive strength of the specimens. Results showed that the failure pattern and compressive strength of the specimens were quite different from the rock and coal samples. The following final failure state characteristics of the combination appeared as the proportion of coal thickness increased: “H”-type tensile failure, “hyperbola”-type co-shear failure and “X”-type shear failure. The values of the compressive strength and longitudinal wave velocity of the combination were between rock and coal and presented a negative exponential function with increasing coal thickness. The compressive strength of the rock body part weakened because of the interface in the coal–rock combination, whereas the coal body part strengthened. Further, a compressive strength calculation model was established for the combined body considering the dual effects of coal–rock size effect and interfacial effect. This study is helpful to explain the mechanical behavior of coal in mining engineering.
This paper focuses on the problem of output-feedback adaptive stabilization for a class of stochastic nonlinear time-delay systems with unknown control directions. First, based on a linear state ...transformation, the unknown control coefficients are lumped together and the original system is transformed to a new system for which control design becomes feasible. Then, after the introduction of an observer, an adaptive neural network (NN) output-feedback control scheme is presented for such systems by using dynamic surface control (DSC) technique and Lyapunov–Krasovskii method. The designed controller ensures that all the signals in the closed-loop system are 4-Moment (or 2-Moment) semi-globally uniformly ultimately bounded. Finally, a numerical example is given to demonstrate the feasibility and effectiveness of the proposed control design.
In order to investigate the compaction and re-crushing characteristics of crushed coal gangue with different gradation, the compaction fractal test was carried out for crushed coal gangue with ...different Talbol power index n. The compaction deformation parameters such as displacement, stress-strain were analyzed according to the test results. And according to the test results, the compaction deformation relationship of the lateral limit crushed coal rock body considering the gradation is obtained. The test results show that: the crushing of coal rock particles exists in almost the whole compaction process of the crushed coal rock body, and the crushing of coal rock particles has a non-negligible influence on the deformation of the whole crushed rock body; the structural stability of large-size coal rock particles is not as good as that of small-size coal rock particles, and the large-size coal rock is more likely to be crushed under the same stress conditions; the distribution coefficient r of the crushed coal rock body decreases with the increase of axial stress; and before the Before the axial stress reaches 8 MPa, the distribution coefficient r of crushed rock samples tends to increase with n in general, and after reaching 8 MPa and later, the distribution coefficient r of crushed rock samples tends to decrease with n in general; the difference value of particle crushing increases with the increase of axial stress, and the weight value of particle crushing decreases with the increase of axial stress, and the changes of both are non-linear; according to the stress recovery in the compaction process of crushed coal rock body The compaction deformation model of the crushed coal rock body is constructed according to the crushing characteristics of coal rock particles in the stage of compaction, which effectively combines its fine action mechanism with macroscopic physical phenomena in a simple form and has certain practical engineering significance.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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