Genu varus (GV) is a common deformity characterized by leg bending, which seriously threatens human health. At present, there is no definite conclusion to explain the cause of genu varus. The purpose ...of this study is to systematically review and meta-analysis the effectiveness and scientific basis of the clinical efficacy of customized orthopedic insoles on genu varus and hope to provide a reference for future research in this field. The following electronic databases will be searched from inception to January 2022: Pubmed, Cochrane Library, MEDLINE, EMBASE, Web of Science, Weipu, Wanfang Data, and CNKI. Randomized controlled trials (RCTs) were collected to examine the effect of lateral wedge-shaped orthopedic insole on patients with genu varus. We will consider inclusion, select high-quality articles for data extraction and analysis, and summarize the intervention effect of lateral wedge orthotic insoles on patients with genu varus. Two reviewers will screen titles, abstracts, and full texts independently according to inclusion criteria; Data extraction and risk of bias assessment were performed in the included studies. We will use a hierarchy of recommended assessment, development, and assessment methods to assess the overall certainty of the evidence and report findings accordingly. Endnote X8 will be applied in selecting the study, Review Manager 5.3 will be applied in analyzing and synthesizing. The results will provide evidence for judging the effect of lateral wedge-shaped orthopedic insole on patients with genu varus. Our study will provide reliable evidence for the effect of lateral wedge-shaped orthopedic insole on patients with genu varus.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Tropomyosin regulates a wide variety of actin filament functions and is best known for the role that it plays together with troponin in controlling muscle activity. For effective performance on actin ...filaments, adjacent 42-nm-long tropomyosin molecules are joined together by a 9- to 10-residue head-to-tail overlapping domain to form a continuous cable that wraps around the F-actin helix. Yet, despite the apparent simplicity of tropomyosin’s coiled-coil structure and its well-known periodic association with successive actin subunits along F-actin, the structure of the tropomyosin cable on actin is uncertain. This is because the conformation of the overlap region that joins neighboring molecules is poorly understood, thus leaving a significant gap in our understanding of thin-filament structure and regulation. However, recent molecular-dynamics simulations of overlap segments defined their overall shape and provided unique and sufficient cues to model the whole actin-tropomyosin filament assembly in atomic detail. In this study, we show that these MD structures merge seamlessly onto the ends of tropomyosin coiled-coils. Adjacent tropomyosin molecules can then be joined together to provide a comprehensive model of the tropomyosin cable running continuously on F-actin. The resulting complete model presented here describes for the first time (to our knowledge) an atomic-level structure of αα-striated muscle tropomyosin bound to an actin filament that includes the critical overlap domain. Thus, the model provides a structural correlate to evaluate thin-filament mechanics, self-assembly mechanisms, and the effect of disease-causing mutations.
Electron microscopy and fiber diffraction studies of reconstituted F-actin-tropomyosin filaments reveal the azimuthal position of end-to-end linked tropomyosin molecules on the surface of actin. ...However, the longitudinal
z-position of tropomyosin along F-actin is still uncertain. Without this information, atomic models of F-actin-tropomyosin filaments, free of constraints imposed by troponin or other actin-binding proteins, cannot be formulated, and thus optimal interfacial contacts between actin and tropomyosin remain unknown. Here, a computational search assessing electrostatic interactions for multiple azimuthal locations,
z-positions, and pseudo-rotations of tropomyosin on F-actin was performed. The information gleaned was used to localize tropomyosin on F-actin, yielding an atomic model characterized by protein-protein contacts that primarily involve clusters of basic amino acids on actin subdomains 1 and 3 juxtaposed against acidic residues on the successive quasi-repeating units of tropomyosin. A virtually identical model generated by docking F-actin and tropomyosin atomic structures into electron microscopy reconstructions of F-actin-tropomyosin validated the above solution. Here, the
z-position of tropomyosin alongside F-actin was defined by matching the seven broad and narrow motifs that typify tropomyosin's twisting superhelical coiled-coil to the wide and tapering tropomyosin densities seen in surface views of F-actin-tropomyosin reconstructions. The functional implications of the F-actin-tropomyosin models determined in this work are discussed.
Deployment of large-scale wind turbines requires sophisticated operation and maintenance strategies to ensure the devices are safe, profitable and cost-effective. Prognostics aims to predict the ...remaining useful life (RUL) of physical systems based on condition measurements. Analyzing condition monitoring data, implementing diagnostic techniques and using machinery prognostic algorithms will bring about accurate estimation of the remaining life and possible failures that may occur. This paper proposes to combine two supervised machine learning techniques, namely, regression model and multilayer artificial neural network model, to predict the RUL of an operational wind turbine gearbox using vibration measurements. Root Mean Square (RMS), Kurtosis (KU) and Energy Index (EI) were analysed to define the bearing failure stages. The proposed methodology was evaluated through a case study involving vibration measurements of a high-speed shaft bearing used in a wind turbine gearbox.
We take a zero tolerance to any situation where fraudulent research is published in our journals. As a result, this article has been retracted by the Publisher because it is suspected to be a ...nonsensical publication computer-generated by the program SCIgen.
•NO3− and NO2− denitrification were confirmed to be coupled to CH4 oxidation.•The nitrogen removal rates were highest in 25% O2 in AME-D3 and 20% O2 in AME-D2.•In ME-SND, the highest NH4+-N removal ...rate reached 22.03 mg/(L•d) in group 25% O2.•The primary methanotrophs in ME-SND were Methylobacter and Methylomonas.•The main HN-AD bacteria in ME-SND were Chryseobacterium and Comamonas.
It would be highly beneficial to use the methane produced by anaerobic digestion, which is low cost and accessible, as the carbon source in the removal of nitrogenous contaminants in wastewater. However, there is a knowledge gap regarding coupling systems that entail methane oxidation, nitrification, and denitrification, which restricts their industrial application. In this study, we acclimated a mixed culture to deal with simultaneous nitrification-denitrification coupled to methane oxidation in a laboratory-scale hollow-fiber membrane biofilm reactor, which achieved a steady ammonia removal rate of 38.09 mg N/(L•d). Furthermore, a series of batch experiments were conducted to test methane oxidation coupled to nitrate denitrification (AME-D3), nitrite denitrification (AME-D2), and simultaneous nitrification and denitrification (ME-SND). The molar ratio between methane consumed and nitrate reduced (C/N) equals 10 and 5 mol CH4C mol−1 NO3N in AME-D3 and AME-D2, averagely and respectively. Without methane injection, the removal of nitrates and nitrites was very low, indicating that the coupling of nitrate/nitrite denitrification and methane oxidation was beneficial. The average ammonia removal rates in the 20% O2 and 25% O2 groups were 20.06 and 22.03 mg N/(L•d) in the ME-SND system, respectively. Without methane, the ammonia oxidation rate declined, and large amounts of nitrite accumulated. As traditional ammonia and nitrite oxidation approaches are autotrophic, we proposed the possibility of heterotrophic nitrification-aerobic denitrification (HN-AD). To study the coupling systems, the microbial communities and functional bacteria were analyzed. The results indicated that the system contained a guild of methanotrophs (mainly Methylobacter) and HN-AD bacteria (mainly Chrysobacterium and Comamonas).
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Transshipment of compacted soybean is characterized by labor-intensive processes, long operating times, and a great amount of dust that cannot be collected by sealed devices. This dust significantly ...reduces air quality of the workshop and represents a great threat to human health. Solving this problem has been a great challenge in the soybean storage industry. For this reason, in the present study the concept of a transshipment system for soybean clearance was proposed. This system can simultaneously achieve real-time transshipment of falling materials and air curtain control of fugitive dust. The main factors influencing the performance of the air curtain dust control system were investigated using numerical simulation, and the effects of the exhaust-to-pressure ratio K and air curtain outlet velocity V on dust control efficiency were determined. Results showed that the width of the dust-collecting paths increased with increasing K and V values. We also found out that dust-escape paths were mainly concentrated in the transition height between the complete air curtains and local air curtains. At the beginning of the process, dust control efficiency rapidly increased and the increment rate became slower as K increased. When K = 1.5 and V = 5–6 m/s, dust control efficiency reached values up to 95.54–96.27%. Based on numerical simulation results, the prototype transshipment system for soybean clearance was developed, and the effectiveness of air curtain dust control was validated via smoke tracing experiments. After the application of the developed system, occupational lung disease can be significantly reduced, and transshipment efficiency per clearance can be enhanced by approximately 67% (about 50,113￥ can be saved in terms of energy consumption and labor resources). An extra granary can be saved after using the newly developed system for 36.5–52.1 clearances.
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•An effective method to solve the soybean blanking dust collection is proposed.•The dust collection method ensures smooth ventilation inside the granary.•Dust collection efficiency reaches 95.54%.•The method is validated by industrial experiments.
miR-155 plays a crucial role in proinflammatory activation. This study was carried out to assess the association of abnormal expression of miR-155 in peripheral blood of patients with Rheumatoid ...arthritis with the expression of TNF-α and IL-1β. Release of TNF-α and IL-1β, and expression of miR-155 were determined in RA peripheral blood or peripheral blood macrophages, followed by correlation analysis of the cytokines release and miR-155 expression. Furthermore, in vitro studies indicate that miR-155 inhibited the expression of SOCS1. Our results suggest that there is a correlation between the high-level expression of miR-155 and the enhanced expression of TNF-α and IL-1β. miR-155 targets and suppresses the expression of SOCS1, and the decrease of SOCS1 may lead to the upregulation of TNF-α and IL-1β.
The inherent flexibility of rod-like tropomyosin coiled-coils is a significant factor that constrains tropomyosin’s complex positional dynamics on actin filaments. Flexibility of elongated straight ...molecules typically is assessed by persistence length, a measure of lengthwise thermal bending fluctuations. However, if a molecule’s equilibrium conformation is curved, this formulation yields an “apparent” persistence length (∼100nm for tropomyosin), measuring deviations from idealized straight conformations which then overestimate actual dynamic flexibility. To obtain the “dynamic” persistence length, a true measurement of flexural stiffness, the average curvature of the molecule must be taken into account. Different methods used in our studies for measuring the dynamic persistence length directly from Molecular Dynamics (MD) simulations of tropomyosin are described here in detail. The dynamic persistence length found, 460±40nm, is ∼12-times longer than tropomyosin and 5-times the apparent persistence length, showing that tropomyosin is considerably stiffer than previously thought. The longitudinal twisting behavior of tropomyosin during MD shows that the amplitude of end-to-end twisting fluctuation is ∼30° when tropomyosin adopts its near-average conformation. The measured bending and twisting flexibilities are used to evaluate different models of tropomyosin motion on F-actin.
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