Exploiting Zn metal‐free anode materials would be an effective strategy to resolve the problems of Zn metal dendrites that severely hinder the development of Zn ion batteries (ZIBs). However, the ...study of Zn metal‐free anode materials is still in their infancy, and more importantly, the low energy density severely limits their practical implementations. Herein, a novel (NH4)2V10O25 · 8H2O@Ti3C2Tx (NHVO@Ti3C2Tx) film anode is proposed and investigated for constructing “rocking‐chair” ZIBs. The NHVO@Ti3C2Tx electrode shows a capacity of 514.7 mAh g−1 and presents low potential which is 0.59 V (vs Zn2+/Zn) at 0.1 A g−1. The introduction of Ti3C2Tx not only affords an interconnected conductive network, but also stabilizes the NHVO nanobelts structure for a long cycle life (84.2% retention at 5.0 A g−1 over 6000 cycles). As a proof‐of‐concept, a zinc metal‐free full battery is successfully demonstrated, which delivers the highest capacity of 131.7 mAh g−1 (mass containing anodic and cathodic) and energy density of 97.1 Wh kg−1 compared to all reported aqueous “rocking‐chair” ZIBs. Furthermore, a long cycling span of 6000 cycles is obtained with capacity retention reaching up to 92.1%, which is impressive. This work is expected to provide new moment toward V‐based materials for “rocking‐chair” ZIBs.
A novel (NH4)2V10O25 · 8H2O@Ti3C2Tx (NHVO@Ti3C2Tx) film anode is proposed and investigated for constructing “rocking‐chair” zinc ion batteries (ZIBs). Due to good crystal and electrode structure engineering, the hybrid electrode exhibits superior electrochemical performance with a low potential (vs Zn2+/Zn). By pairing the NHVO@ Ti3C2Tx film anode and ZnMn2O4 cathode, the Zn‐ion full battery can deliver remarkable specific capacity and energy density.
Soil microbial communities affect nutrient cycling and ecosystem functioning. However, the variations in microbial diversity and community composition within degraded landscapes remain unclear. Using ...high‐throughput sequencing of bacterial 16S ribosomal RNA genes and internal transcribed spacer fungal sequences, we investigated the rhizosphere microbial diversity and community of coniferous Pinus tabulaeformis Carr. forests in degraded lands across a chronosequence that spanned over 60 years (10, 25, 40, and 60 years since restoration, four forest stands). We found significant differences in soil bacterial and fungal communities among stand ages. Actinobacteria, Proteobacteria, and Acidobacteria dominated the rhizosphere, whereas Basidiomycota, Ascomycota, and Zygomycota prevailed as fungal components. With stand development, bacterial diversity decreased, but fungal diversity increased. Nonmetric multidimensional scaling analysis separated bacterial community clusters well by stands. Fungal community clusters of 25‐ and 60‐year‐old stands overlapped. The dominant bacteria Acidobacteria showed the highest relative abundance at the 40‐year‐old stands. Soil microbial communities correlated significantly with the macro‐nutrients (soil organic carbon, total nitrogen, and total phosphorous). Additionally, the relative abundance of Acidobacteria at the phylum level correlated positively with soil total phosphorous; Deltaproteobacteria at the class level correlated positively with soil organic carbon and total nitrogen. Thus, restoring vegetation in degraded temperate forests enhanced some macronutrients and influenced microbial communities. Our results revealed that restoring vegetation in degraded lands decreased the diversity of bacterial communities over time. In contrast, the soil fungal diversity increased after restoration, and fungal communities in the 25‐ and 60‐year‐old forest stands overlapped on degraded soils.
Objective To explore the effect of a health (E)-coach chronic disease management model on the rehabilitation behaviour management of patients with arteriosclerosis obliterans (ASO). Methods The ...E-coach chronic disease management model was constructed based on a literature review and expert interviews. The effect of the E-coach model on patients with ASO during hospitalisation was analysed by comparing the compliance rates of blood glucose control, blood pressure control, drug compliance, ankle-brachial index, 6-min walking test (6MWT) and pain-free walking distance (PFWD) scores between the E-coach and control groups. Results In total, 212 patients with ASO were included in this study. After the intervention, the blood pressure compliance rate (44.8% vs. 65.7%) and blood glucose compliance rate (48.6% vs. 66.8%) were higher in the E-coach group than in the control group (p < 0.05). After intervention, compared with the control group, the patients in the E-coach group had better drug compliance (6.8 + or - 1.9 vs. 7.9 + or - 1.0), and the difference was statistically significant (p < 0.05). The scores for the 6MWT (329.19 + or - 5.58 vs. 353.00 + or - 9.76; 412.65 + or - 12.59 vs. 499.16 + or - 18.43) and PFWD (219.15 + or - 11.96 vs. 225.36 + or - 16.13; 331.62 + or - 51.36 vs. 369.42 + or - 75.71) tests were significantly higher in the E-coach group than in the control group at 1 and 6 months after intervention (p < 0.05). Conclusion The E-coach chronic disease management model can effectively improve the control rates of blood glucose and blood pressure and the behaviour management of patients with ASO and is thus worthy of clinical reference. Keywords: Chronic disease management mode, Health coaching technology, Continuous nursing, Arteriosclerosis obliterans
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, ODKLJ, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK, VSZLJ
In recent years, host-microbiome interactions in both animals and plants has emerged as a novel research area for studying the relationship between host organisms and their commensal microbial ...communities. The fitness advantages of this mutualistic interaction can be found in both plant hosts and their associated microbiome, however, the driving forces mediating this beneficial interaction are poorly understood. Alternative splicing (AS), a pivotal post-transcriptional mechanism, has been demonstrated to play a crucial role in plant development and stress responses among diverse plant ecotypes. This natural variation of plants also has an impact on their commensal microbiome. In this article, we review the current progress of plant natural variation on their microbiome community, and discuss knowledge gaps between AS regulation of plants in response to their intimately related microbiota. Through the impact of this article, an avenue could be established to study the biological mechanism of naturally varied splicing isoforms on plant-associated microbiome assembly.
Polymer nanocomposites with high energy density have become a research hotspot in the field of dielectric materials. However, the huge compatibility contrast between nanofillers and polymers always ...hinders the further improvement of dielectric properties. Meanwhile, next-generation dielectrics should possess excellent thermal stability to cope with the development of high-temperature applications. Herein, a novel polymer nanocomposite based on heat-resistant poly( p -phenylene benzobisoxazole) (PBO) and high-permittivity CaCu 3 Ti 4 O 12 (CCTO) nanoparticles was prepared by the solution method. In this process, electroactive polymer poly(2-isopropenylbenzoxazole) (P(2-IBO)), which has a chemical structure similar to PBO and contains numerous permanent dipoles, is selected as the modifier to optimize the electric field behavior at the interface, and it is coated on the surface of CCTO nanoparticles via surface-initiated reversible addition–fragmentation chain transfer (RAFT) polymerization for achieving controllable dielectric properties. The results demonstrate that the dielectric properties can be adjusted by changing the thickness of the P(2-IBO) shell. The breakdown strength and energy density of the modified CCTO/PBO nanocomposites are much higher than those of the unmodified CCTO/PBO nanocomposites. Under an electric field of 200 kV mm −1 , the maximum discharge energy density reaches 3 J cm −3 , which is about 500% that of pure PBO (0.6 J cm −3 ). Moreover, the dielectric properties are nearly independent of the testing temperature (25 to 200 °C), indicating that this nanocomposite is an ideal candidate for high-temperature dielectric materials.
Summary
Despite a much higher proportion of intragenic heterochromatin‐containing genes in crop genomes, the importance of intragenic heterochromatin in crop development remains unclear. Intragenic ...heterochromatin can be recognised by a protein complex, ASI1–AIPP1–EDM2 (AAE) complex, to regulate alternative polyadenylation.
Here, we investigated the impact of rice ASI1 on global poly(A) site usage through poly(A) sequencing and ASI1‐dependent regulation on rice development.
We found that OsASI1 is essential for rice pollen development and flowering. OsASI1 dysfunction has an important impact on global poly(A) site usage, which is closely related to heterochromatin marks. Intriguingly, OsASI1 interacts with the intronic heterochromatin of OsXRNL, a nuclear XRN family exonuclease gene involved in the processing of an miRNA precursor, to promote the processing of full‐length OsXRNL and regulate miRNA abundance. We found that OsASI1‐mediated regulation of pollen development partially depends on OsXRNL. Finally, we characterised the rice AAE complex and its involvement in alternative polyadenylation and pollen development.
Our findings help to elucidate an epigenetic mechanism governing miRNA abundance and rice development, and provide a valuable resource for studying the epigenetic mechanisms of many important processes in crops.
We aimed to identify an unique host transcriptional signature in peripheral blood mononuclear cells (PBMCs) in response to
antigens to distinguish between patients with leprosy and non-leprosy ...controls for early diagnosis of the disease. Sixteen individuals were enrolled in the discovery cohort eight patients with leprosy, comprising four multibacillary (MB) and four paucibacillary (PB); and eight non-leprosy controls, comprising four healthy house contacts (HHCs) and four endemic controls (ECs). The differences in the transcriptome response of PBMCs to
sonicate antigen were evaluated between leprosy patients and non-leprosy controls, and 12 differentially expressed genes (
,
,
,
,
,
,
,
,
,
, and
) were identified. The accuracy of the 12 differentially expressed genes was further validated for the diagnosis of leprosy using real-time quantitative PCR in 82 individuals (13 MB, 10 PB, 37 HHCs, and 22 ECs) in the validation cohort. We found that a 5 gene signature set
,
,
,
and
had a suitable performance in discriminating leprosy from ECs. In addition, elevated expression of
,
,
and
was associated with MB diagnosis compared with ECs, whereas increased expression of
,
,
and
was found to be useful biomarkers for PB diagnosis from ECs. Moreover, we found decreased expression of
among leprosy patients could distinguish leprosy from HHCs, whereas higher expression of
among MB than PB could distinguish different leprosy patients. In conclusion, among the 12 candidate host genes identified, a three gene signature
, and
showed the best performance in distinguishing leprosy patients from healthy controls. These findings may have implications for developing a rapid blood-based test for early diagnosis of leprosy.
4-Hydroxyphenylpyruvate dioxygenase (HPPD, EC 1.13.11.27) is an important target site for discovering new bleaching herbicides. To explore novel HPPD inhibitors with excellent herbicidal activity, a ...series of novel N-aroyl diketone/triketone derivatives were rationally designed by splicing active groups and bioisosterism. Bioassays revealed that most of these derivatives displayed preferable herbicidal activity against Echinochloa crus-galli (EC) at 0.045 mmol/m2 and Abutilon juncea (AJ) at 0.090 mmol/m2. In particular, compound I-f was more potent compared to the commercialized compound mesotrione. Molecular docking indicated that the corresponding active molecules of target compounds and mesotrione shared similar interplay with surrounding residues, which led to a perfect interaction with the active site of Arabidopsis thaliana HPPD.
The cellular mechanisms of primary varicose great saphenous veins (GSVs) involve inflammation, apoptosis, and proliferation of local cells and extracellular matrix degradation. Long non-coding RNAs ...(lncRNAs) play important roles in these cellular processes; however, which and how lncRNAs related to these mechanisms take effect on GSVs remain unclear. By screening lncRNAs that might experience changes in GSV varicosities, we selected the lower expressed lncRNA-GAS5 (growth arrest specific transcript 5) for functional assessments. Silencing of lncRNA-GAS5 promoted cell proliferation and migration, and cell cycle of the human saphenous vein smooth muscle cells (HSVSMCs), whereas overexpressing it inhibited these cellular behaviors and reduced apoptosis of HSVSMCs. RNA pull-down experiment revealed a direct bind of lncRNA-GAS5 to a Ca2+-dependent RNA-binding protein, Annexin A2. Further experiments showed that silencing of Annexin A2 reduced the HSVSMCs proliferation and vice versa. In the context of lncRNA-GAS5 knockdown, silencing of Annexin A2 reduced the proliferation of HSVSMCs while overexpression of Annexin A2 increased the proliferation. Thus, the low expression of lncRNA-GAS5 may facilitate HSVSMCs proliferation and migration through Annexin A2 and thereby the pathogenesis of GSV varicosities.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Invertebrates rely heavily on immune-like molecules with highly diversified variability so as to counteract infections. However, the mechanisms and the relationship between this variability and ...functionalities are not well understood. Here, we showed that the C-terminal domain of hemocyanin (HMC) from shrimp
contained an evolutionary conserved domain with highly variable genetic sequence, which is structurally homologous to immunoglobulin (Ig). This domain is responsible for recognizing and binding to bacteria or red blood cells, initiating agglutination and hemolysis. Furthermore, when HMC is separated into three fractions using anti-human IgM, IgG, or IgA, the subpopulation, which reacted with anti-human IgM (HMC-M), showed the most significant antimicrobial activity. The high potency of HMC-M is a consequence of glycosylation, as it contains high abundance of α-d-mannose relative to α-d-glucose and
-acetyl-d-galactosamine. Thus, the removal of these glycans abolished the antimicrobial activity of HMC-M. Our results present a comprehensive investigation of the role of HMC in fighting against infections through genetic variability and epigenetic modification.
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