Late blight seriously threatens potato cultivation worldwide. The severe and widespread damage caused by the fungal pathogen can lead to drastic decreases in potato yield. Although grafting ...technology has been widely used to improve crop resistance, the effects of grafting on potato late blight resistance as well as the associated molecular mechanisms remain unclear. Therefore, we performed RNA transcriptome sequencing analysis and the late blight resistance testing of the scion when the potato late blight-resistant variety Qingshu 9 and the susceptible variety Favorita were used as the rootstock and scion, respectively, and vice versa. The objective of this study was to evaluate the influence of the rootstock on scion disease resistance and to clarify the related molecular mechanisms. A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the expression levels of genes related to plant-pathogen interactions, plant mitogen-activated protein kinase (MAPK) signaling pathways, and plant hormone signal transduction pathways were significantly up-regulated in the scion when Qingshu 9 was used as the rootstock. Some of these genes encoded calcium-dependent protein kinases (CDPKs), chitin elicitor receptor kinases (CERKs), LRR receptor serine/threonine protein kinases (LRR-LRKs), NPR family proteins in the salicylic acid synthesis pathway, and MAPKs which were potato late blight response proteins. When Favorita was used as the rootstock, only a few genes of late blight response genes were upregulated in the scion of Qingshu 9. Grafted plants using resistant variety as rootstocks inoculated with P. infestans spores showed significant reductions in lesion size while no significant difference in lesion size was observed when susceptible variety was used as the rootstock. We also showed that this induction of disease resistance in scions, especially scions derived from susceptible potato varieties was mediated by the up-regulation of expression of genes involved in plant disease resistance in scions. Our results showed that potato grafting using late blight resistant varieties as rootstocks could render or enhance resistance to late blight in scions derived from susceptible varieties via up-regulating the expression of disease resistant genes in scions. The results provide the basis for exploring the molecular mechanism underlying the effects of rootstocks on scion disease resistance.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
China's agricultural economy is developing rapidly, but the unbalanced regional development is still a key issue that needs to be discussed today. By studying the total factor productivity of green ...agriculture and its factors, this paper analyzes the regional differences in time and space changes between the eastern, central and western parts of China. In this paper, the total factor productivity of green agriculture is calculated and decomposed by Metafrontier Malmquist-Luenberger index based on directional distance function. The results are as follows: First, the total factor productivity level of green agriculture in China is increasing year by year, but the overall level is still at a low level and has greater volatility; Second, although the total factor productivity of green agriculture shows an upward trend, the three regions show a downward trend in turn, which has great differences; Third, there are obvious differences in technological efficiency, optimal production potential and technological gap between the eastern, central and western regions, and there are great differences in productivity among regions and provinces. Based on the results, this paper puts forward policy recommendations, according to the regional heterogeneity, from a number of angles to rely on the joint efforts of many parties to improve the level of total factor productivity of green agriculture.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abstract
As an important embodiment of a country's economic strength and national health, pharmaceutical manufacturing industry has made rapid development in China in recent years. But at the same ...time, the pharmaceutical manufacturing industry is facing many environmental problems, such as large pollution emissions, complex pollution components, controlling difficulties and so on. This paper measures the total factor productivity of pharmaceutical manufacturing industry (HTFP) by using data envelopment analysis with unexpected output, which is more accurate and effective than the traditional model. It also studies the effect of environmental regulation on the total factor productivity of pharmaceutical manufacturing industry (HTFP) by establishing panel data regression model and spatial econometric model based on 30 provinces in China from 2004 to 2019, which enriches the research results in the field of cleaning in pharmaceutical manufacturing industry. The conclusions are as follows: (1) Environmental regulation and total factor productivity of pharmaceutical manufacturing industry have significant spatial autocorrelation, showing "high-high" or "low-low" spatial aggregation characteristics; (2) Environmental regulation has a significant promoting effect on improving pharmaceutical manufacturing total factor productivity in local and surrounding areas, and there are differences in the impact of eastern, central and western regions; (3) Green technology, production technology and industrial structure play an important role in the impact of environmental regulation on pharmaceutical manufacturing total factor productivity, which provides theoretical guidance and policy recommendations for improving the level of total factor productivity of pharmaceutical manufacturing industry in the environmental aspect.
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•Ultrasound pretreatment promoted the subsequent glycation reaction.•Conjugates pretreated by 400 W ultrasound exhibited optimal water solubility.•The increased net charge induced ...dissociation of myofibrillar proteins (MPs).•The ultrasound–glycation combination inhibited filament formation in low-salt media.
The poor water solubility of myofibrillar proteins (MPs) limits their application in food industry, and is directly related to the molecular behavior associated with myosin assembly into filaments. This study aims to explore the effect of high-intensity ultrasound (HIU) combined with nonenzymatic glycation on the solubility, structural characteristics, and filament-forming behavior of MPs in low ionic strength media. The results showed that the HIU (200–400 W) application could promote the subsequent glycation reaction between MPs and dextran (DX) and interfere with the electrostatic balance between myosin rods, suppressing the formation of filamentous myosin polymers. Glycated MPs pretreated by 400 W HIU had the highest solubility, which corresponded to the smallest particle size, highest zeta potential, and optimum storage stability (P < 0.05). Structure analysis and microscopic morphology observations suggested that the loss of the MP superhelix and the depolymerization of filamentous polymers were the main mechanisms for MP solubilization. In conclusion, HIU combined with glycation can effectively improve the water solubility of MPs by destroying or suppressing the assembly of myosin molecules.
As China’s strategic support belt, the green development of industry in the Yangtze River Economic Zone is of great significance to promote the construction of China’s ecological civilization, build ...a modern industrial system and accelerate high-quality economic development. The study of green total factor productivity of industry in the Yangtze River Economic Zone has important theoretical and practical value for exploring the green development path of China’s industry. This Paper takes the Yangtze River Economic Zone, a key strategic region in China, as the research object, selects the input and output data of industrial production from 2006 to 2018, based on DEA model. To construct an MML index considering expected and unexpected output, and to quantitatively analyze the changes of industrial GTFP in the Yangtze River Economic Zone. The results show that: (1) During the sample period, the industrial green total factor productivity in the Yangtze River Economic Zone shows the spatial characteristics of differential growth and the temporal characteristics of volatile growth. It shows the fluctuation characteristics of “N” shape. (2) According to the order of "upper, middle, and lower" reaches, the spatial pattern of industrial green total factor productivity is characterized by "lower ladder". But the difference between the upper and middle reaches is small. (3) Cities with higher green total factor productivity and lower green total factor productivity each form the characteristics of "club convergence" of spatial agglomeration. (4) Technological efficiency and technological progress efficiency have heterogeneous effects on different river basins in the upper, middle, and lower reaches, and technological progress efficiency is conducive to promoting the evolution of green total factor productivity to a high level. According to the above empirical results, this paper finally puts forward the policy recommendations to improve the industrial green total factor productivity of the Yangtze River Economic Zone and the policy recommendations to reduce the industrial differences between the Yangtze River Economic Zone.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Most existing recommendation models only consider single user–item interaction information, which leads to serious cold-start or data sparsity problems. In practical applications, a user’s behavior ...is multi-type, and different types of user behavior show different semantic information. To achieve more accurate recommendations, a major challenge comes from being able to handle heterogeneous behavior data from users more finely. To address this problem, this paper proposes a multi-behavior recommendation framework based on a graph neural network, which captures personalized semantics of specific behavior and thus distinguishes the importance of different behaviors for predicting the target behavior. Meanwhile, this model establishes dependency relationships among different types of interaction behaviors under the graph-based information transfer network, and the graph convolutional network is further used to capture the high-order complexity of interaction graphs. The experimental results of three benchmark datasets show that the proposed graph-based multi-behavior recommendation model displays significant improvements in recommendation accuracy compared to the baseline method.
Club (Clara) cell protein 16 (CC-16) is a protein that is synthesized predominantly in the lungs and is detectable in serum. Its expression decreases with lung injury and smoking, and is thus a ...marker of bronchial cell dysfunction.
To evaluate the possibility of using serum CC-16 as a biomarker for disease progression in chronic obstructive pulmonary disease (COPD).
We measured serum CC-16 levels from 4,724 subjects with mild-to-moderate airflow limitation in the Lung Health Study. Using a linear regression model, we determined the relationship of serum CC-16 concentrations to decline in lung function over 9 years. In addition, to determine whether CC-16 plays a major role in the pathogenesis of mild COPD, we exposed CC-16-deficient (-/-) mice to 6 months of cigarette smoke.
Reduced serum concentrations of CC-16 were associated with accelerated decline in FEV1 over 9 years (P < 0.0001), and this association persisted after adjustments for age, sex, race, smoking status, airway reactivity, body mass index, and baseline FEV1 (P = 0.0002). However, CC-16(-/-) mice did not demonstrate an enhanced risk of emphysema or small airway remodeling in response to cigarette smoke.
Serum CC-16 is associated with disease progression, and may assist in the identification of "rapid progressors." However, the absence of CC-16 does not appear to modify the risk of cigarette-related COPD in mice.
As2O3 and resveratrol have been widely considered to be effective in anti-cancer therapies and the underlying mechanisms have been reported extensively. However, the combined treatment effect and ...potential target of As2O3 and resveratrol in the treatment of tumors remains elusive. The purpose of this study was to investigate the benefits and efficacy of As2O3 in combination with resveratrol in the treatment of colon cancer, as well as looking for new targets that could provide alternative explanation of the efficacy of drugs.
The proliferation of cancer cells was measured by the MTT and EdU staining assay, while the apoptosis of cancer cells was determined by the flow cytometry. Western blot and immunoprecipitation were performed to measure the expression levels of proteins and the interaction between hERG and integrin β1, respectively.
In this study, we found that both As2O3 and resveratrol can effectively inhibit cell proliferation and promote cell apoptosis in colon cancer, and the combined effect of the two drugs on colon cancer cells is more preeminent. The combination of As2O3 with resveratrol, on the one hand reduced the expression of hERG channels on the membrane, and on the other hand weaken the binding between hERG and integrin β 1, which may be the main cause of downstream signaling pathways alterations, including the activation of the apoptotic pathway.
Taken together, hERG, as a subunit of potassium ion channel on the cell membrane, is highly likely to be involved in the As2O3 and resveratrol induced intracellular signaling cascade disorder, and this novel signaling pathway that sustains the progression of colon cancer may be a promising therapeutic target for human colon cancer treatment in the future.
Although estrogens exert a pronounced protective effect on multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE), their therapeutic application has been limited by ...undesirable side effects thought to be mediated primarily through estradiol binding to intracellular estrogen receptor alpha. In this study, we found that signaling through the putative membrane estrogen receptor, G protein-coupled receptor 30 (GPR30), was sufficient to mediate protection against EAE, which was significantly impaired in GPR30 gene-deficient mice. Treatment with G-1, an agonist that selectively activates GPR30 without engagement of the intracellular estrogen receptors, retained the ability of estradiol to protect against clinical and histological EAE without estradiol-associated side effects, deviated cytokine profiles, and enhanced suppressive activity of CD4(+)Foxp3(+) T regulatory cells through a GPR30- and programmed death 1-dependent mechanism. This study is the first to evaluate the protective effect of GPR30 activation on EAE, and provides a strong foundation for the clinical application of GPR30 agonists such as G-1 in multiple sclerosis.
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•An experimental method that can achieve different deceleration was designed.•A constitutive model was developed to describe the dynamic response of the PBX.•The material parameters ...of the HMX/F2602 were obtained.•The dynamic response of PBX was studied from macroscopic and mesoscopic perspectives.
Based on the experimental method of projectile penetration into the concrete target, different excitation by deceleration of polymer bonded explosives (PBXs) was achieved by controlling the length of steel pillars behind PBXs. A series of impact tests were conducted to study the mechanical response of PBXs with different initial densities under different excitation by deceleration. A physical model was developed to describe the viscoelastic-plastic deformation of PBXs. The plastic part was added on the basis of the viscoelastic statistical crack mechanical model (Visco-SCRAM) model, and the compaction behavior of charge in different environments was characterized based on the Heckel compaction equation. The model was implemented in LS-DYNA, and the simulation results were verified by the experiments. The dynamic mechanical behavior of PBXs under different excitation by deceleration was studied from the macroscopic and mesoscale aspects. The stress curves, strain curves, the density change and the interaction between particles in deceleration environment were predicted. After comparison and analysis, the dynamic response law of the charge in the penetrating process was obtained. Macroscopic responses along with mesoscale modeling results are significantly important to have a better understanding of deformation mechanisms of PBXs.
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