This article puts forward a constructivist-interpretivist approach to interface conflicts that emphasises how international actors articulate and problematise norm collisions in discursive and social ...interactions. Our approach is decidedly agency-oriented and follows the Special Issue’s interest in how interface conflicts play out at the micro-level. The article advances several theoretical and methodological propositions on how to identify norm collisions and the conditions under which they become the subject of international debate. Our argument on norm collisions, understood as situations in which actors perceive two norms as incompatible with each other, is threefold. First, we claim that agency matters to the analysis of the emergence, dynamics, management, and effects of norm collisions in international politics. Second, we propose to differentiate between dormant (subjectively perceived) and open norm collisions (intersubjectively shared). Third, we contend that the transition from dormant to open – which we term activation – depends on the existence of certain scope conditions concerning norm quality as well as changes in power structures and actor constellations. Empirically, we study norm collisions in the area of international drug control, presenting the field as one that contains several cases of dormant and open norm collisions, including those that constitute interface conflicts. For our in-depth analysis we have chosen the international discourse on coca leaf chewing. With this case, we not only seek to demonstrate the usefulness of our constructivist-interpretivist approach but also aim to explain under which conditions dormant norm collisions evolve into open collisions and even into interface conflicts.
During COVID-19, various public institutions tried to shape citizens' behaviour to slow the spread of the pandemic. How did their authority affect citizens' support of public measures taken to combat ...the spread of COVID-19? The article makes two contributions. First, it presents a novel conceptualisation of authority as a source heuristic. Second, it analyses the authority of four types of public institutions (health ministries, universities, public health agencies, the WHO) in two countries (Germany and the UK), drawing on novel data from a survey experiment conducted in May 2020. On average, institutional endorsements seem to have mattered little. However, there is an observable polarisation effect where citizens who ascribe much expertise to public institutions support COVID-19 measures more than the control group. Furthermore, those who ascribe little expertise support them less than the control group. Finally, neither perception of biases nor exposure to institutions in public debates seems consistently to affect their authority.
In recent years, stem cell-derived organoids have become a cell culture standard that is widely used for studying various scientific issues that were previously investigated through animal ...experiments and using common tumor cell lines. After their initial hype, concerns regarding their standardization have been raised. Here, we aim to provide some insights into our experience in standardizing murine colonic epithelial organoids, which we use as a replacement method for research on inflammatory bowel disease. Considering good scientific practice, we examined various factors that might challenge the design and outcome of experiments using these organoids. First, to analyze the impact of antibiotics/antimycotics, we performed kinetic experiments using ZellShield® and measured the gene expression levels of the tight junction markers Ocln, Zo-1, and Cldn4, the proliferation marker Ki67, and the proinflammatory cytokine Tnfα. Because we found no differences between cultivations with and without ZellShield®, we then performed infection experiments using the probiotic Escherichia coli Nissle 1917 as an already established model setup to analyze the impact of technical, interexperimental, and biologic replicates. We demonstrate that interexperimental differences pose the greatest challenge for reproducibility and explain our strategies for addressing these differences. Additionally, we conducted infection experiments using freshly isolated and cryopreserved/thawed organoids and found that cryopreservation influenced the experimental outcome during early passages. Formerly cryopreserved colonoids exhibited a premature appearance and a higher proinflammatory response to bacterial stimulation. Therefore, we recommend analyzing the growth characteristics and reliability of cryopreserved organoids before to their use in experiments together with conducting several independent experiments under standardized conditions. Taken together, our findings demonstrate that organoid culture, if standardized, constitutes a good tool for reducing the need for animal experiments and might further improve our understanding of, for example, the role of epithelial cells in inflammatory bowel disease development.
Cd14 and Alpk1 both encode pathogen recognition receptors and are known candidate genes for affecting severity in inflammatory bowel diseases. CD14 acts as a coreceptor for bacterial ...lipopolysaccharide (LPS), while ALPK1 senses ADP-D-glycero-beta-D-manno-heptose, a metabolic intermediate of LPS biosynthesis. Intestinal barrier integrity can be influenced by CD14, whereas to date, the role of ALPK1 in maintaining barrier function remains unknown. We used colon-derived 3D organoids, first characterised for growth, proliferation, stem cell markers, and expression of tight junction (TJ) components using qPCR and immunohistochemistry. They showed characteristic crypt stem cells, apical shedding of dead cells, and TJ formation. Afterwards, organoids of different genotypes (WT, Il10-/-, Cd14-/-, and Alpk1-/-) were then stimulated with either LPS or Escherichia coli Nissle 1917 (EcN). Gene expression and protein levels of cytokines and TJ components were analysed. WT organoids increased expression of Tnfα and tight junction components. Cd14-/- organoids expressed significantly less Tnfα and Ocln after LPS stimulation than WT organoids but reacted similarly to WT organoids after EcN stimulation. In contrast, compared to WT, Alpk1-/- organoids showed decreased expression of different TJ and cytokine genes in response to EcN but not LPS. However, Western blotting revealed an effect of ALPK1 on TJ protein levels. These findings demonstrate that Cd14, but not Alpk1, alters the response to LPS stimulation in colonic epithelial cells, whereas Alpk1 is involved in the response upon bacterial challenge.
Der Beitrag setzt sich würdigend und kritisch mit Michael Zürns Arbeiten zur internationalen Autorität auseinander. Dessen potenziell autoritatives Autoritätskonzept weist mehrere Vorzüge auf: ...Erstens bietet es eine Erklärung für ein Paradox. Warum sollten souveräne Staaten die Kompetenz Externer anerkennen, ihnen Ratschläge zu geben bzw. Forderungen an sie zu richten, und zudem noch bereit sein, diesen zu folgen? Zweitens konkretisiert es die u.a. bei Hannah Arendt angelegte Idee der fraglosen Anerkennung, indem es Autoritätsadressaten zugesteht, bestimmte Qualitäten der Autorität zu prüfen. Drittens entkoppelt es Legitimität und Autorität, ohne die Legitimationsbedürftigkeit von Autorität zu opfern. Dies anerkennend plädiert der Beitrag aber dafür, die Legitimationsbedürftigkeit internationaler Autorität nicht auf formal institutionalisierte Beziehungen zu reduzieren, sondern diese auch weiterhin auf informellere, d.h. der Praxis entstammende, Anerkennung und Folgebereitschaft innerhalb von Autoritätsbeziehungen zu beziehen. Die überzeugende begründungstheoretische Fundierung von Autorität sollte zudem nicht dazu verführen, Sozialisationsprozesse in Autoritätsbeziehungen zu übersehen, zumal deren Legitimität kritisch hinterfragbar ist.
This article pays tribute to and critically engages with the works of Michael Zürn on international authority. His potentially authoritative concept of reflexive authority contains multiple advantages: First, it explains the paradox of why sovereign states should recognize or even defer to external advice. Second, it adds nuance to Hannah Arendt’s idea of unquestioned recognition, by including the capabilities of the authority takers to examine the quality of authority (holders). Third, it decouples legitimacy and authority without, however, sacrificing the necessity of authority to be legitimized. In light of these important contributions, this article argues that the analysis of legitimation demands should not be reduced to formal, institutionalized relationships of authority. Instead, it calls for a continued inclusion of informal, practice-based recognition and deference. Furthermore, while the reason-based social foundation of authority is convincing, it should not result in overlooking processes of socialization in authority relationships.
Der Beitrag setzt sich würdigend und kritisch mit Michael Zürns Arbeiten zur internationalen Autorität auseinander. Dessen potenziell autoritatives Autoritätskonzept weist mehrere Vorzüge auf: ...Erstens bietet es eine Erklärung für ein Paradox. Warum sollten souveräne Staaten die Kompetenz Externer anerkennen, ihnen Ratschläge zu geben bzw. Forderungen an sie zu richten, und zudem noch bereit sein, diesen zu folgen? Zweitens konkretisiert es die u.a. bei Hannah Arendt angelegte Idee der fraglosen Anerkennung, indem es Autoritätsadressaten zugesteht, bestimmte Qualitäten der Autorität zu prüfen. Drittens entkoppelt es Legitimität und Autorität, ohne die Legitimationsbedürftigkeit von Autorität zu opfern. Dies anerkennend plädiert der Beitrag aber dafür, die Legitimationsbedürftigkeit internationaler Autorität nicht auf formal institutionalisierte Beziehungen zu reduzieren, sondern diese auch weiterhin auf informellere, d.h. der Praxis entstammende, Anerkennung und Folgebereitschaft innerhalb von Autoritätsbeziehungen zu beziehen. Die überzeugende begründungstheoretische Fundierung von Autorität sollte zudem nicht dazu verführen, Sozialisationsprozesse in Autoritätsbeziehungen zu übersehen, zumal deren Legitimität kritisch hinterfragbar ist.
This article pays tribute to and critically engages with the works of Michael Zürn on international authority. His potentially authoritative concept of reflexive authority contains multiple advantages: First, it explains the paradox of why sovereign states should recognize or even defer to external advice. Second, it adds nuance to Hannah Arendt’s idea of unquestioned recognition, by including the capabilities of the authority takers to examine the quality of authority (holders). Third, it decouples legitimacy and authority without, however, sacrificing the necessity of authority to be legitimized. In light of these important contributions, this article argues that the analysis of legitimation demands should not be reduced to formal, institutionalized relationships of authority. Instead, it calls for a continued inclusion of informal, practice-based recognition and deference. Furthermore, while the reason-based social foundation of authority is convincing, it should not result in overlooking processes of socialization in authority relationships.
Der Beitrag setzt sich würdigend und kritisch mit Michael Zürns Arbeiten zur internationalen Autorität auseinander. Dessen potenziell autoritatives Autoritätskonzept weist mehrere Vorzüge auf: ...Erstens bietet es eine Erklärung für ein Paradox. Warum sollten souveräne Staaten die Kompetenz Externer anerkennen, ihnen Ratschläge zu geben bzw. Forderungen an sie zu richten, und zudem noch bereit sein, diesen zu folgen? Zweitens konkretisiert es die u.a. bei Hannah Arendt angelegte Idee der fraglosen Anerkennung, indem es Autoritätsadressaten zugesteht, bestimmte Qualitäten der Autorität zu prüfen. Drittens entkoppelt es Legitimität und Autorität, ohne die Legitimationsbedürftigkeit von Autorität zu opfern. Dies anerkennend plädiert der Beitrag aber dafür, die Legitimationsbedürftigkeit internationaler Autorität nicht auf formal institutionalisierte Beziehungen zu reduzieren, sondern diese auch weiterhin auf informellere, d.h. der Praxis entstammende, Anerkennung und Folgebereitschaft innerhalb von Autoritätsbeziehungen zu beziehen. Die überzeugende begründungstheoretische Fundierung von Autorität sollte zudem nicht dazu verführen, Sozialisationsprozesse in Autoritätsbeziehungen zu übersehen, zumal deren Legitimität kritisch hinterfragbar ist.
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