Astaxanthin, a xanthophyll belonging to the family of carotenoids, is a potent antioxidant. However, much less is known about its protective effects on the oxidative stress of ischemic optic nerve. ...We hypothesized that astaxanthin treatment could protect retinal ganglion cells (RGCs) from death via anti-oxidative and anti-apoptotic responses. Adult male Wistar rats were fed astaxanthin (100 mg/kg/day) by daily gavage for seven consecutive days, either before or after inducing oxidative stress in the retina by photodynamic treatment. The visual function, RGC apoptosis, macrophage infiltration in the optic nerve, expression of p-Akt, p-mTOR, SGK1, pS6K, Nrf2, p62, TNFα, Il1β in retinas were investigated. The visual function and the RGC densities were significantly higher in both pre- and post-treatment groups. The numbers of apoptotic RGCs and extrinsic macrophage infiltration in the optic nerve were significantly decreased in both astaxanthin-treated groups. Furthermore, pre- and post-treatment of astaxanthin showed a higher expression of p-Akt, p-mTOR, Nrf2 and superoxide dismutase activity, and a lower expression of cleaved caspase-3, suggesting anti-apoptotic and anti-oxidative roles. Our findings indicate that astaxanthin can preserve visual function and reduce RGC apoptosis after ischemic insults. Including astaxanthin in daily diet as a supplement may be beneficiary for ischemic optic neuropathy.
Background
Colorectal ESD is difficult because of the poor maneuverability and difficulty of mucosal flap creation. Diving, Lifting and Horizontal (DLH) dissection technique and loop-clip traction ...are two different methods to facilitate mucosal trimming and adequate mucosal flap creation. We combined the advantages of these two techniques (DLH+T) in our daily practice colorectal ESD since July 2020.
Objective
The purpose of this study was to examine the outcomes of DLH+T dissection compared with the conventional dissection.
Methods
We retrospectively reviewed the clinical using DLH+T dissection compared with the conventional dissection since January 2018 at a single tertiary care institution. Postoperative short-term outcomes were investigated after the procedure including mucosal flap creation time, dissection time, dissection speed, en bloc resection rate, and perioperative complications.
Results
28 lesions were in DLH+T dissection group and 39 lesions in the conventional dissection group. The outcomes including en bloc resection rate, dissection speed, and complication between the two groups were similar. The mean mucosal flap creation time (
p
= 0.035) and the mean dissection speed (
p
= 0.041) of the DLH+T dissection group was significantly shorter and faster.
Conclusion
DLH dissection followed by loop-clip traction (DLH+T) technique is a useful technique for safe, efficient, and adequate mucosal flap creation, which can increase the dissection speed and may prevent complication, especially in biopsy-related submucosal fibrosis.
High‐efficiency organic light emitting diodes (OLEDs) are fabricated using a group of benzimidazole‐carbazole derivatives with good molecular packing as hosts. The hosts are separately doped with a ...green thermally activated delayed fluorescence (TADF) emitter, 1,2,3,5‐tetrakis(carbazol‐9‐yl)‐4,6‐dicyanobenzene (4CzIPN). An ultralow concentration (0.5% volume ratio) of 4CzIPN is required in the emitting layer (EML) to achieve a record‐high external quantum efficiency of 31.8% by comparison with reported 4CzIPN‐relative devices. This result is attributed to efficient energy transfer, alleviation of concentration quench of 4CzIPN, long‐distance triplet exciton diffusion ability of host materials, and excellent horizontal molecular packing. With an increase in dopant concentration from 0.5% to 15%, the diodes exhibit a tiny variation in power efficiencies (82.9−78.7 lm W−1) and current efficiencies (92.2−87.5 cd A−1). In particular, the long‐distance triplet exciton diffusion ability of hosts exceeds the typical distance limitation (less than 10 Å) of Dexter energy transfer. Thus, high‐efficiency OLEDs are obtained with the scarce dopants . The aforementioned long‐distance triplet diffusion results are verified by the almost‐identical high‐efficiency device performances and the longer delayed emission lifetime in transient electroluminescence signals of a series of partially doped devices, conducting by separately doping low‐concentration TADF emitters in different EML regions.
A series of benzimidazole‐carbazole compounds exhibit long‐distance triplet exciton diffusion ability and excellent molecular packing. Employing as host and doped with an ultralow 4CzIPN concentration (0.5% volume ratio), a record‐high external quantum efficiency (31.8%) of a green thermally activated delayed fluorescence organic light‐emitting diode (TADF‐OLED) with o‐3CbzBz host is demonstrated.
Background Chemoradiotherapy (CRT), which might affect the autonomic system, is the mainstay therapy for advanced esophageal squamous cell carcinoma (ESCC). Autonomic dysfunction has been found to ...possibly lead to cancer mortality in those with elevated resting heart rates (RHR). Skin sympathetic nerve activity (SKNA) is a new method of stimulating electrical signals in skin to evaluate autonomic function from sympathetic tone. In this study, we investigated the association between changes in RHR and autonomic function and ESCC mortality. Methods Thirty-nine stage II-IV ESCC patients receiving CRT between March 2019 and November 2020 were prospectively enrolled and carefully selected, followed up and received the same meticulous supportive care. Serial RHR was recorded every two weeks from before CRT to eight weeks after CRT and average SKNA were recorded before and four weeks after CRT. All-cause mortality was defined as primary outcome. Results We found the RHR of ESCC patients to be significantly elevated and peaking at four weeks after CRT (p < 0.001) and then to gradually decrease. Those with an elevated RHR above the cutoff level (18 beat-per-minute) at eight weeks after CRT had worse overall survival. In addition, those with higher baseline sympathetic tone (average SKNA greater than or equal to 0.86 muV) also had poor outcome. Conclusions Increased pre-treatment sympathetic tone and elevated RHR after CRT are alarm signs of poor ESCC outcome. Further exploration of the mechanisms underlying these associations could potentially lead to intervention strategies for reducing mortality. Trial registration This study is registered with ClinicalTrials.gov, identifier: NCT03243448. Keywords: Esophageal squamous cell carcinoma, Chemoradiotherapy, Resting heart rate, Skin sympathetic nerve activity, Autonomic nerve system, Survival
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Exciton dynamics in a solid-state exciplex sensitized triplet-triplet annihilation (ESTTA) system are studied using transient photoluminescence (TrPL) measurements. The ESTTA system is a trilayer ...structure with 4,4',4''-tris(N-3-methyphenyl-N-phenyl-amino)triphenylamine (m-MTDATA) acting as the electron donor, 1-(2,5-dimethyl-4-(1-pyrenyl)phenyl)pyrene (DMPPP) as a triplet-diffusion-singlet-blocking (TDSB) layer, and 9,10-bis(2'-naphthyl) anthracene (ADN), acting as the electron acceptor and emitter. The thicknesses of the m-MTDATA and ADN layers are 30 nm, while the thickness of the DMPPP layer is varied to characterize its effect on the singlet quenching of the ADN emission. We find that electron transfer via tunneling through the DMPPP layer is the dominant quenching channel, with a characteristic length of ∼5 nm. Doping the high photoluminescence quantum yield molecule 4,4'-bis2-(4-(N,N-diphenylamino)phenyl)vinylbiphenyl (DPAVBi) into the ADN layer enhanced the overall intensity of the ESTTA signal but did not prevent quenching by exciplex formation. The trilayer configuration (m-MTDATA/DMPPP/ADN) can effectively prevent ADN singlets from being quenched by electron transfer and exciplex formation, and a key property of the DMPPP is its tendency to not undergo electron transfer to the ADN.
An Fe(III)-promoted oxidative annulation reaction was developed for the synthesis of 1,2-naphthoquinones. A variety of substituted arylglyoxals and internal alkynes undergo the transformation in the ...presence of FeCl3 at room temperature to afford the 1,2-naphthoquinone products in good yields in a short reaction time. Interestingly, the products show unusual pseudomigration of the substituent on the arene ring of arylglyoxals. A possible mechanism involving Fe(III)-promoted formation of a vinyl cation from arylglyoxal and alkyne, electrophilic addition of the vinyl cation to the ipso carbon of the aryl group to give a spiral intermediate, and then migration of the keto carbon to the ortho carbon was proposed as key steps and verified using quantum mechanics.
This study investigated the kinetics of estimated glomerular filtration rate (eGFR) and quantitative hepatitis B surface antigen (qHBsAg) in telbivudine (LdT)-treated chronic hepatitis B (CHB) ...patients whose treatment was subsequently adjusted with the adding on adefovir or by switching to tenofovir disoproxil fumarate (TDF) as rescue. Of 295 CHB patients initially treated with LdT, 102 of them who subsequently receiving either adding-on adefovir (group A, n = 58) or switching to TDF (group B, n = 44) for more than 24 months were enrolled. Serial eGFR and qHBsAg levels (3 to 6 monthly) in both LdT monotherapy and rescue therapy periods were analyzed retrospectively. Subsequent decline of qHBsAg especially in rescue therapy period were noted (p0.001 and p = 0.068 in group A and B). However, patients in group B achieved a significant increase of eGFR (p = 0.010) in LdT monotherapy period but had a significant decline of eGFR (p20% from baseline. Both rescue therapies achieved subsequent declines of qHBsAg over time but caused different changes in eGFR. LdT-based rescue therapy maintained eGFR but TDF switching therapy descended eGFR. Therefore, it is essential to monitor patients renal function intensively when switching from LdT to TDF as a rescue strategy.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Cisplatin-based chemotherapy is the primary treatment for metastatic bladder urothelial carcinoma (UC). Most patients inevitably encounter drug resistance and resultant disease relapse. Reduced ...apoptosis plays a critical role in chemoresistance. Trifluoperazine (TFP), an antipsychotic agent, has demonstrated antitumor effects on various cancers. This study investigated the efficacy of TFP in inhibiting cisplatin-resistant bladder UC and explored the underlying mechanism. Our results revealed that cisplatin-resistant UC cells (T24/R) upregulated the antiapoptotic factor, B-cell lymphoma-extra large (Bcl-xL). Knockdown of Bcl-xL by siRNA resensitized cisplatin-resistant cells to the cisplatin cytotoxic effect. TFP (10-45 μM) alone elicited dose-dependent cytotoxicity, apoptosis, and G0/G1 arrest on T24/R cells. Co-treatment of TFP potentiated cisplatin-induced cytotoxicity in T24/R cells. The phenomenon that TFP alleviated cisplatin resistance to T24/R was accompanied with concurrent suppression of Bcl-xL. In vivo models confirmed that TFP alone effectively suppressed the T24/R xenograft in nude mice. TFP co-treatment enhanced the antitumor effect of cisplatin on the T24/R xenograft. Our results demonstrated that TFP effectively inhibited cisplatin-resistant UCs and circumvented cisplatin resistance with concurrent Bcl-xL downregulation. These findings provide a promising insight to develop a therapeutic strategy for chemoresistant UCs.
Summary
In this study, a laser headlight driving controller (LHDC) using the controller area network (CAN)‐bus communication system was developed and implemented for vehicle headlight applications. ...To observe temperature variations that influence the forward‐bias voltage and optical power of laser diodes, the laser diode was placed in an ambient temperature‐testing chamber to measure the electro‐optic characteristics and calculate the electro‐optic conversion efficiencies. The optical power and conversion efficiency values obtained at different temperatures (from negative to positive temperatures) when optical power adjustment was executed using analog and pulse‐width modulation technologies were compared. Moreover, a human‐machine operating interface was developed using the LabVIEW software. Therefore, the LHDC and laser optical power can be controlled using a computer through the CAN‐bus communication system to control the LHDC and monitor it.
The CAN‐bus port of the MCU with the CAN‐bus transceiver in the LHDC enables communication with the computer. When the computer commands the LHDC, the duty cycle ratio of the PDCS can be controlled by the MCU to adjust the OP of the BBLD.
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