The double-corrugated-plate shear wall (DCPSW) is composed of two identical corrugated plates that are assembled using connecting bolts. The DCPSW can exhibit much greater shear resistance than the ...ordinary steel corrugated plate shear wall (SCPSW) since the former forms a series of closed-sections while the latter is with open sections. In this paper, the shear resistant behavior of the DCPSWs is investigated. By using a validated FE modeling scheme, a parametric study of aspect ratio, bolt column number, corrugated plate thickness and yield stress of the DCPSWs is performed to investigate their effects on the shear resistant behavior. By analyzing the FE results, the ultimate shear resistance factor φ and the residual strength factor φr which corresponds to a final state with a drift angle of 2.0% of the DCPSW are obtained. It is found that both factors are negatively related to the normalized slenderness ratio λn. The φ-λn and φr-λn relationships obtained in this study are capable of providing conservative predictions of the ultimate shear resistance and post-ultimate residual strength for the DCPSWs. Finally, the shear resistant curves between shear load and drift angle of the DCPSWs with the variable of the normalized slenderness ratio λn are established. These shear resistant curves are regarded as simplified constitutive models when performing the required push-over elastoplastic analysis of the DCPSWs under severe earthquake excitation.
•DCPSWs achieve better stability behavior under shear loads over ordinary CPSWs.•A validated nonlinear model is used to investigate the shear resistant behavior.•A parametric study is performed to study the effects of wall dimensions on its shear resistant behavior.•Formulas are proposed to perform conservative predictions of shear resistance and residual strength.•A procedure for predicting the shear resistant curves is proposed.
In this paper, finite‐time cluster synchronization (FTCS) in networks of nonidentical coupled dynamical systems is addressed by introducing a discontinuous interaction rule. This interaction rule ...overcomes the effect of heterogeneous dynamics among nodes. It is proven that with proper coupling gain, the dynamics of the coupled system converges into the kernal space of the graph Laplacian matrix in finite time. Then sufficient conditions for FTCS are provided. In addition, the discontinuous interactions rule is applied to investigate finite‐time intra‐layer synchronization in multiplex networks.
The finite‐time cluster synchronization problem in networks of nonidentical coupled dynamical systems are solved by introducing a discontinuous interaction rule. In this paper, both the uncoupled dynamics of nodes and the coupling structure are general. Finite‐time intra‐layer synchronization of multiplex networks is also discussed.
Due to the changes in the surrounding environment, the dynamic of one degradation process may change at a random time, and it follows different modes before and after the change point. For solving ...online degradation state estimation problems subject to random change of mode, a novel state estimation method is proposed in this article based on the degradation models and the related monitored data. The proposed method employs the sequential probability ratio test (SPRT) based on the log-likelihood ratio to detect the unknown change time of the degradation mode and particle filtering to estimate the degradation states given observations and also evaluate the decision functions of the SPRT. Two case studies referring to a pneumatic valve considering single- and multiple-change times of the degradation mode are presented to illustrate the accuracy and effectiveness of the proposed method.
In recent years, circular RNAs (circRNAs) have been found to play an essential regulatory role in hepatocellular carcinoma (HCC) through various mechanisms, particularly the endogenous competitive ...RNA (ceRNA) mechanism. Therefore, it is significant to explore the circRNAs in hepatoma. In this study, we constructed the ceRNA and survival network using Cytoscape. We also used R, Perl software, and multiple online databases and platforms, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), to perform overall survival, immune cell infiltration, immune checkpoints, pathway activity, and anticancer drug sensitivity analysis of the genes. Finally, the receiver operator characteristic curve (ROC) analysis was performed to identify the diagnosis value of the genes. KEGG analysis revealed the T cell receptor signaling pathway as the main enrichment pathway. A total of 29 genes related to survival and prognosis were screened out. The findings suggest that ZNF544, WDR76, ACTG1, RASSF3, E2F3, ASRGL1, and POGK are associated with multilevel immune cell infiltration. Additionally, immune checkpoint analysis screened out the ACTG1, E2F3, RASSF3, and WDR76. It was also revealed that the WDR76, E2F3, ASRGL1, and POGK mainly activated the cell cycle and DNA damage response (DDR) pathway. The results suggest that the sensitivity toward trametinib, refametinib (RDEA119), and selumetinib correlates to the expression of WDR76. ROC analysis showed that the area under the curve (AUC) of all genes in the regulatory axis was greater than 0.7. The identified hsa_circ_0000417/hsa_circ_0002688/hsa_circ_0001387--hsa-miR-199a-5p--WDR76 regulatory axis may provide new insights into the progression, clinical diagnosis, and treatment of HCC.
Gliomas are the most common malignant tumors of the brain and are characteristic of severe migration and invasion. Glial cell line-derived neurotrophic factor (GDNF) promotes glioma development ...process. However, the regulatory mechanisms of promoting occurrence and development of glioma have not yet been clearly elucidated. In the present study, the mechanism by which GDNF promotes glioma cell migration and invasion through regulating the dispersion and location of the Golgi apparatus (GA) is described. Following GDNF treatment, a change in the volume and position of GA was observed. The stack area of the GA was enlarged and it was more concentrated near the nucleus. Golgin-160 and Golgi microtubule-associated protein 210 (GMAP210) were identified as target molecules regulating GA positioning. In the absence of either golgin-160 or GMAP210 using lentivirus, the migration and invasion of U251 cells were decreased, while it was increased following GDNF. It was also found that the GA was decreased in size and dispersed following golgin-160 or GMAP210 knockdown, as determined by GA green fluorescence assay. Once GDNF was added, the above phenomenon would be twisted, and the concentrated location and volume of the GA was restored. In combination, the present data suggested that the regulation of the position and size of the GA by golgin-160 and GMAP210 play an important role in U251 cell migration and invasion.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Process alarm systems and process monitoring play a significant role in ensuring stability and safety in industrial production. The continual increase in the complexity and scale of industrial ...processes makes it difficult to monitor processes online and accurately track the root causes of alarms. To address these problems, online distributed process monitoring and alarm analysis using novel canonical variate analysis (CVA) with multicorrelation blocks (MCB-CVA) and enhanced contribution plot is developed in this paper. The proposed methodology is simple: first, process knowledge is used to divide the entire system into multicorrelation blocks to enhance fault detection performance; an improved contribution plot method based on principal component analysis is adopted as an alarm analysis strategy such that the distributed contribution can be used for online analysis; finally, a model of distributed alarm analysis is developed using the novel MCB-CVA and enhanced contribution plot. The performance of the proposed methodology is tested using case studies based on the Tennessee Eastman process (TEP). The simulation results show that the proposed MCB-CVA can achieve good online performance in not only fault detection but also root cause analysis, proving the practicability and effectiveness of the proposed MCB-CVA model.
Transcription activator-like effector nucleases (TALENs) are a powerful approach for targeted genome editing and have been proved to be effective in several organisms. In this study, we reported that ...TALENs can induce somatic mutations in Nile tilapia, an important species for worldwide aquaculture, with reliably high efficiency. Six pairs of TALENs were constructed to target genes related to sex differentiation, including dmrt1, foxl2, cyp19a1a, gsdf, igf3, and nrob1b, and all resulted in indel mutations with maximum efficiencies of up to 81% at the targeted loci. Effects of dmrt1 and foxl2 mutation on gonadal phenotype, sex differentiation, and related gene expression were analyzed by histology, immunohistochemistry, and real-time PCR. In Dmrt1-deficient testes, phenotypes of significant testicular regression, including deformed efferent ducts, degenerated spermatogonia or even a complete loss of germ cells, and proliferation of steroidogenic cells, were observed. In addition, disruption of Dmrt1 in XY fish resulted in increased foxl2 and cyp19a1a expression and serum estradiol-17β and 11-ketotestosterone levels. On the contrary, deficiency of Foxl2 in XX fish exhibited varying degrees of oocyte degeneration and significantly decreased aromatase gene expression and serum estradiol-17β levels. Some Foxl2-deficient fish even exhibited complete sex reversal with high expression of Dmrt1 and Cyp11b2. Furthermore, disruption of Cyp19a1a in XX fish led to partial sex reversal with Dmrt1 and Cyp11b2 expression. Taken together, our data demonstrated that TALENs are an effective tool for targeted gene editing in tilapia genome. Foxl2 and Dmrt1 play antagonistic roles in sex differentiation in Nile tilapia via regulating cyp19a1a expression and estrogen production.
Four phosphorescent cyclometalated iridium(III) complexes containing benzimidazole moiety have been designed and synthesized. These Ir(III) complexes can effectively inhibit several cancerous ...processes, including cell migration, invasion, colony formation, and angiogenesis. Interestingly, they show a much higher singlet oxygen quantum yield in an acidic solution than in a neutral solution. Upon irradiation at 425 nm with low energy (1.2 J cm–2), they can induce apoptosis through lysosomal damage, evaluation of reactive oxygen species level, and activation of caspase-3/7. The highest phototoxicity index is >476, with almost no dark cytotoxicity observed for Ir4. Ir4 can also inhibit tumor growth effectively in nude mice in vivo after photodynamic therapy. An in vitro assay against 70 kinases indicates that maternal embryonic leucine zipper kinase (MELK), PIK3CA, and AMPK are the possible molecular targets. The half maximal inhibitory concentration of Ir4 toward MELK is 1.27 μM. Our study demonstrates that these Ir(III) complexes are promising anticancer agents with dual functions, including metastasis inhibition and lysosome-damaged photodynamic therapy.
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•We demonstrate PffBT4T-2OD:PC71BM based high-efficient organic photodiodes (OPD).•We show the application of OPD in blue-light hazard detection (BLH).•The OPD shows an EQE of greater ...than 70%, a value of Jd of 1.18 × 10–9 A cm−2.•The OPD exhibits a high detectivity (2.9 × 1013 Jones) with ultrafast response.•Our design allows the ratio of blue-violet and blue-green light to be determined.
In this study we incorporated a PffBT4T-2OD:PC71BM bulk-heterojunction (BHJ) active layer in the layer structure ITO/ZnO/BHJ/MoO3/Ag, obtaining high-performance organic photodiodes (OPDs) for application in blue-light hazard (BLH) detection. The high crystallinity of PffBT4T-2OD resulted in the OPD displaying high performance even when the thickness of the BHJ layer was up to 850 nm. The efficient OPD suppressed charge injection (from the metal contacts), thereby providing state-of-the-art performance: external quantum efficiencies of greater than 70% (380–710 nm), a value of Jd of 1.18 × 10-9 A cm−2 (-2 V), and high detectivity 2.9 × 1013 Jones, −2 V (710 nm). The observed ultra-high cut-off frequency greater than800 kHz (at 530 nm), with rise/fall times of less than 350 ns at reverse biases of less than −3 V exceeded that of a silicon photodiode and made the system suitable for use in image photosensors and medical monitoring. The wide linear dynamic range (LDR, 530 nm) of the OPD (139 dB at −1 V, from 2.2 × 10-4 to 2.5 × 10-11 A cm−2) encompassed the mesopic and scotopic vision regimes. Combined with the design of a BLH filter, we calculated the environmental BLH weighted irradiation power density WDBLH = CB × JSC (current density of diode) to evaluate the BLH effect for various light sources and commercially available lenses; here CB (12.69, reported herein) is a corrective constant for BLH photodetectors. Finally, our design allowed ready determination of the ratio of blue-violet and blue-green light.
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