Considering the “furnace-caster matching” modes, this paper focuses on the scheduling problems from practical steelmaking-continuous casting production lacking refining span. Aiming at the ...improvement on quality and output of steel products, a mathematical model is established with multi-objective optimization including the minimum earliness/tardiness of starting cast times, the shortest waiting times of heats among different processes and the shortest idle times of converters. A heuristic algorithm based on the optimization of “furnace-caster matching” mode is developed to solve this model, which involves two procedures of device assignment and conflict elimination. Through the detailed analysis on workshop layout and production rhythm, four classes of matching modes of “refining furnace-caster” are proposed to perform the assignments of refining furnaces. The assignments of converters rely on three categories of greedy strategies in terms of minimizing conflictions among heats. A rough scheduling solution with some possible conflicts among heats is obtained through combining “furnace-caster matching” modes and greedy strategies. Then applying the linear programming method to eliminate the conflicts and generate the final solution. Based on the proposed algorithm and the improved genetic algorithms, simulation experiments are carried out by introducing actual production plans as instances. The results indicate that heuristic algorithm based on the optimization of “furnace-caster matching” mode is the right candidate owing to its acceptable scheduling solutions with the better process matching relations and the highlighted performances under crane constraint. Currently, the proposed model and algorithm have been successfully used in a large converter steel plant in China.
This paper is concerned with the general regular long-wave (RLW) types of equations. By the combination of Painlevé analysis and Lie group classification method, the conditional Painlevé property ...(PP) and Bäcklund transformations (BTs) of the nonlinear wave equations are provided under some conditions. Then, all of the point symmetries of the nonlinear RLW types of equations are obtained, the exact solutions to the equations are investigated. Particularly, some explicit solutions are provided by the special function and Φ-expansion method.
Graphical abstract
Lithium–sulfur (Li–S) batteries have received increasing attention due to their high energy density. However, it is still challenging to inhibit the diffusion of polysulfides and achieve high sulfur ...utilization. Herein, we designed and prepared a free-standing and binder-free electrode for high-performance Li–S batteries by in situ growth of three-dimensional ordered macroporous MoO2 on carbonized nonwoven cloth (3DOM MoO2/CC). After the uptake of Li2S6, the obtained MoO2/CC–Li2S6 electrode with a high sulfur loading of 3.26 mg cm−2 delivers a large discharge capacity of 1267 mA h g−1 at 0.1C. A high discharge capacity of 621 mA h g−1 is still retained after 500 cycles at 2C. The excellent electrochemical performance of this MoO2/CC–Li2S6 electrode is attributed to the unique nanostructure and strong chemical interaction between MoO2 and polysulfides. 3DOM MoO2 not only guarantees the high loading of sulfur but also suppresses the diffusion of polysulfides. The carbonized nonwoven cloth (CC) functions as the basic support for 3DOM MoO2, enhancing the electronic conductivity and mechanical properties of the free-standing electrodes. This work provides a feasible strategy for the construction of high-performance free-standing binder-free Li–S electrodes.
Previous studies have identified
miR-182
,
miR-27a
,
FoxO1
, and
IL2RA
as regulatory factors for Treg cell development and function. In order to investigate the association of miR-182, miR-27a,
FoxO1
..., and
IL2RA
gene polymorphisms with Behçet’s disease (BD) and Vogt–Koyanagi–Harada (VKH) syndrome in a Chinese Han population, a two-stage association study was performed in 820 BD, 900 VKH patients, and 1,800 controls using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. In the first stage study, association analysis of 10 single nucleotide polymorphisms (SNPs) was performed in 400 BD, 400 VKH patients, and 600 controls. The results showed significantly decreased frequencies of the miR-182/rs76481776 CC genotype and C allele in BD (
P
= 3.36 × 10
−4
, OR = 0.55;
P
= 4.74 × 10
−4
, OR = 0.59) and VKH patients (
P
= 1.11 × 10
−4
, OR = 0.53;
P
= 1.26 × 10
−4
, OR = 0.56). No significant association of the other nine SNPs with BD or VKH was observed. In the second stage study, association analysis of miR-182/rs76481776 was performed in 420 BD, 500 VKH patients, and 1,200 controls. The second stage and combined studies confirmed the association of miR-182/rs76481776 with BD (CC genotype:
P
= 3.25 × 10
−7
, OR = 0.58; C allele:
P
= 1.81 × 10
−7
, OR = 0.60) and VKH (CC genotype:
P
= 7.89 × 10
−8
, OR = 0.57; C allele:
P
= 2.52 × 10
−8
, OR = 0.59). Real-time PCR analysis showed a significantly increased expression of miR-182 in TT/CT cases compared to CC cases in anti-CD3/CD28 antibodies-stimulated CD4
+
T cells (
P
= 2.1 × 10
−2
). In conclusion, this study suggests that miR-182, but not miR-27a,
FoxO1
, and
IL2RA
, contributes to the genetic susceptibility of BD and VKH.
Key Message
MiR-182 contributes to genetic susceptibility of BD and VKH.
No significant association of miR-27a, FoxO1, and IL2RA with BD or VKH was observed.
Significantly increased expression of miR-182 in TT/CT cases compared to CC cases was observed.
The metal-organic framework MOF-5 was synthesized by self-assembling of Zn(NO3)2·7H2O and H2BDC using DMF as solvent by the direct precipitation method and loaded with Fe2+ by the equivalent loading ...method at room temperature to prepare Fe(II)/MOF-5 catalyst and the microstructure, phases, and pore size of which was characterized by IR, XRD, SEM, TEM, and BET. It was found that Fe(II)/MOF-5 had high specific surface and porosity like MOF-5 and uniform pore distribution, and the pore size is 1.2 nm. In order to study the catalytic activity and reaction conditions of Fe(II)/MOF-5, it was used to catalyze the hydroxylation reaction of phenol with hydrogen peroxide. The results showed that the dihydroxybenzene yield of 53.2% and the catechol selectivity of 98.6% were obtained at the Fe2+ content of 3 wt.%, the mass ratio of Fe(II)/MOF-5 to phenol of 0.053, the reaction temperature of 80°C, and the reaction time of 2 h.
Common wild rice (Oryza rufipogon Griff.), the progenitor of Asian cultivated rice (O. sativa L.), is endangered due to habitat loss. The objectives of this research were to evaluate the genetic ...diversity of wild rice species in isolated populations and to develop a core collection of representative genotypes for ex situ conservation. We collected 885 wild rice accessions from eight geographically distinct regions and transplanted these accessions in a protected conservation garden over a period of almost two decades. We evaluated these accessions for 13 morphological or phenological traits and genotyped them for 36 DNA markers evenly distributed on the 12 chromosomes. The coefficient of variation of quantitative traits was 0.56 and ranged from 0.37 to 1.06. SSR markers detected 206 different alleles with an average of 6 alleles per locus. The mean polymorphism information content (PIC) was 0.64 in all populations, indicating that the marker loci have a high level of polymorphism and genetic diversity in all populations. Phylogenetic analyses based on morphological and molecular data revealed remarkable differences in the genetic diversity of common wild rice populations. The results showed that the Zengcheng, Gaozhou, and Suixi populations possess higher levels of genetic diversity, whereas the Huilai and Boluo populations have lower levels of genetic diversity than do the other populations. Based on their genetic distance, 130 accessions were selected as a core collection that retained over 90% of the alleles at the 36 marker loci. This genetically diverse core collection will be a useful resource for genomic studies of rice and for initiatives aimed at developing rice with improved agronomic traits.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Uncovering genetic variation through resequencing is limited by the fact that only sequences with similarity to the reference genome are examined. Reference genomes are often incomplete and cannot ...represent the full range of genetic diversity as a result of geographical divergence and independent demographic events. To more comprehensively characterize genetic variation of pigs (
), we generated de novo assemblies of nine geographically and phenotypically representative pigs from Eurasia. By comparing them to the reference pig assembly, we uncovered a substantial number of novel SNPs and structural variants, as well as 137.02-Mb sequences harboring 1737 protein-coding genes that were absent in the reference assembly, revealing variants left by selection. Our results illustrate the power of whole-genome de novo sequencing relative to resequencing and provide valuable genetic resources that enable effective use of pigs in both agricultural production and biomedical research.
Esophageal squamous cell carcinoma (ESCC) is malignant while the carcinogenesis is still unclear. Here, we perform a comprehensive multi-omics analysis of 786 trace-tumor-samples from 154 ESCC ...patients, covering 9 histopathological stages and 3 phases. Proteogenomics elucidates cancer-driving waves in ESCC progression, and reveals the molecular characterization of alcohol drinking habit associated signatures. We discover chromosome 3q gain functions in the transmit from nontumor to intraepithelial neoplasia phases, and find TP53 mutation enhances DNA replication in intraepithelial neoplasia phase. The mutations of AKAP9 and MCAF1 upregulate glycolysis and Wnt signaling, respectively, in advanced-stage ESCC phase. Six major tracks related to different clinical features during ESCC progression are identified, which is validated by an independent cohort with another 256 samples. Hyperphosphorylated phosphoglycerate kinase 1 (PGK1, S203) is considered as a drug target in ESCC progression. This study provides insight into the understanding of ESCC molecular mechanism and the development of therapeutic targets.
This study examined the neuroprotective properties of resveratrol (Res) and its target sirtuin1 (SIRT1) against lead (Pb)-mediated toxicity and discovered that both resveratrol treatment and SIRT1 ...overexpression restored blocked autophagic flux as well as reduced β-amyloid (Aβ) contents. Four-week-old male C57BL/6 mice were employed to consumed 0.2% Pb(Ac)2 solution or deionized water for 3 months followed by 12 months of Res (50 mg/kg BW) or vehicle gavage. In in vitro study, SH-SY5Y cells were pretreated with the SIRT1 activator SRT1720 (2 μM) or the inhibitor EX527 (2 μM) for 2 h, then 25 μM of Pb(Ac)2 was added and incubated for 48 h. Western blotting, RT-qPCR, enzyme-linked immunosorbent assay (ELISA), and Lyso-Tracker Red Staining were next used to estimate the potential alterations of the autophagic pathway as well as BACE1-mediated amyloid processing in response to Pb exposure, respectively. Our data revealed that Res treatment or SIRT1 activation resisted the induction of autophagy by Pb exposure through inhibition of LC3 and Beclin-1 expression and promoted the degradation of Aβ and Tau phosphorylation. Besides, the SIRT1 activator (SRT1720) downregulated the expression of BACE1, the rate-limiting enzyme for Aβ production, by inhibiting the activation of nuclear factor-κB (NF-κB) in Pb-treated SH-SY5Y cells, which resulted in reduced Aβ production. Collectively, we verified the role of Res-SIRT1-autophagy as well as the SIRT1-NF-κB-BACE1 pathway in Pb-induced neuronal cell injury by in vivo or in vitro models. Our findings further elucidate the important role of SIRT1 and Res in counteracting Pb neurotoxicity, which may provide new interventions and targets for the subsequent treatment of neurodegenerative diseases.
•Resveratrol mitigated mice hippocampal Aβ level and Tau phosphorylation in response to early-life lead exposure.•Resveratrol or SRT1720 rescued lead-mediated neuronal autophagic dysfunction in vivo and in vitro.•Activation of SIRT1 by SRT1720 suppressed NF-κB/BACE1 signaling, thereby attenuating Aβ1–40 production in case of lead exposure.
Microcystins are frequently detected in cyanobacterial bloom-impacted sites; however, their mobility potential in soils is poorly understood. This study aimed to elucidate the sorption behaviors of ...microcystin-RR (MC-RR) in heterogeneous soils and evaluate critical affecting factors. MC-RR sorption followed the pseudo-second-order kinetics and Freundlich model. All isotherms (n = 0.83–1.03) had no or minor deviations from linearity. The linear distribution coefficients (Kd) varied from 2.64 to 15.2 across soils, depending mainly on OM and CEC. Stepwise regression analysis indicated that the Kd was predictable by the fitting formula of: Kd = 2.56 + 0.15OM + 0.28CEC (R2 = 0.45). The sorption was an endothermic physisorption process, involving electrostatic forces, cation exchange and bridging, H-bonding, ligand exchange, and van der Waals forces. The sorption of MC-RR (dominantly behaved as electroneutral zwitterions) at pH > 5 was insensitive to pH change, while more MC-RR (anionic species) was adsorbed at lower pH and in the presence of Ca2+. The study provides insights into the sorption of MC-RR across a range of soil properties and water chemistry for the first time, which is of importance for a better understanding of the mobility potential of microcystins in the terrestrial systems.
Display omitted
•MC-RR sorption onto soils follows the pseudo-second-order kinetics and Freundlich isotherms models.•Distribution coefficients for MC-RR varied across soils, depending mainly on OM and CEC.•Water chemistry complicates the sorption via influencing the chemical species and confirmation of MC-RR.•The favorable sorption occurs at lower pH and in the presence of Ca2+.
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