Gastrointestinal microbiota may be involved in
associated gastric cancer development. The aim of this study was to explore the possible microbial mechanisms in gastric carcinogenesis and potential ...dysbiosis arising from
infection.
Deep sequencing of the microbial 16S ribosomal RNA gene was used to investigate alterations in paired gastric biopsies and stool samples in 58 subjects with successful and 57 subjects with failed anti-
treatment, relative to 49
negative subjects.
In
positive subjects, richness and Shannon indexes increased significantly (both p<0.001) after successful eradication and showed no difference to those of negative subjects (p=0.493 for richness and p=0.420 for Shannon index). Differential taxa analysis identified 18 significantly altered gastric genera after eradication. The combination of these genera into a Microbial Dysbiosis Index revealed that the dysbiotic microbiota in
positive mucosa was associated with advanced gastric lesions (chronic atrophic gastritis and intestinal metaplasia/dysplasia) and could be reversed by eradication. Strong coexcluding interactions between
and
,
,
,
,
were found only in advanced gastric lesion patients, and were absent in normal/superficial gastritis group. Changes in faecal microbiota included increased
after successful
eradication and more upregulated drug-resistant functional orthologs after failed treatment.
infection contributes significantly to gastric microbial dysbiosis that may be involved in carcinogenesis. Successful
eradication potentially restores gastric microbiota to a similar status as found in uninfected individuals, and shows beneficial effects on gut microbiota.
•The methods for analyzing phosphate starvation-induced secreted APase activity were provided.•A brief historical review on the identification of phosphate starvation-induced APase was provided.•The ...functions of phosphate starvation-induced APase were reviewed.•The molecular mechanisms that regulate phosphate starvation-induced APase were reviewed.•A perspective on future direction in this field was discussed.
Phosphorus is essential for plant growth and development, but levels of inorganic phosphate (Pi), the major form of phosphorus that plants assimilate, are quite limiting in most soils. To cope with Pi deficiency, plants trigger a suite of adaptive responses, including the induction and secretion of acid phosphatases (APases). In this article, we describe how Pi starvation-induced (PSI) APases are analyzed, and we provide a brief historical review of their identification. We then discuss the current understanding of the functions of PSI-secreted APases and how these APases are regulated at the molecular level. Finally, we provide a perspective on the future direction of research in this field.
Yes‐associated protein (YAP) is a component of the canonical Hippo signaling pathway that is known to play essential roles in modulating organ size, development, and tumorigenesis. Activation or ...upregulation of YAP1, which contributes to cancer cell survival and chemoresistance, has been verified in different types of human cancers. However, the molecular mechanism of YAP1 upregulation in cancer is still unclear. Here we report that the E3 ubiquitin ligase STUB1 ubiquitinates and destabilizes YAP1, thereby inhibiting cancer cell survival. Low levels of STUB1 expression were correlated with increased protein levels of YAP1 in human gastric cancer cell lines and patient samples. Moreover, we revealed that STUB1 ubiquitinates YAP1 at the K280 site by K48‐linked polyubiquitination, which in turn increases YAP1 turnover and promotes cellular chemosensitivity. Overall, our study establishes YAP1 ubiquitination and degradation mediated by the E3 ligase STUB1 as an important regulatory mechanism in gastric cancer, and provides a rationale for potential therapeutic interventions.
Our study establishes YAP1 ubiquitination and degradation mediated by the E3 ligase STUB1 as an important regulatory mechanism in gastric cancer, and provides a rationale for potential therapeutic interventions.
A statistical shape-constrained reconstruction (SSCR) framework is presented to incorporate the statistical prior information of human lung shapes for lung electrical impedance tomography. The prior ...information is extracted from 8000 chest-computed tomography scans across 800 patients. The reconstruction framework is implemented with two approaches-a one-step SSCR and an iterative SSCR in lung imaging. The one-step SSCR provides fast and high accurate reconstructions of healthy lungs, whereas the iterative SSCR allows to simultaneously estimate the pre-injured lung and the injury lung part. The approaches are evaluated with the simulated examples of thorax imaging and also with the experimental data from a laboratory setting, with difference imaging considered in both the approaches. It is demonstrated that the accuracy of lung shape reconstruction is significantly improved. In addition, the proposed approaches are proved to be robust against measurement noise, modeling error caused by inaccurately known domain boundary, and the selection of the regularization parameters.
It is well established that ferroptosis is primarily induced by peroxidation of long-chain poly-unsaturated fatty acid (PUFA) through nonenzymatic oxidation by free radicals or enzymatic stimulation ...of lipoxygenase. Although there is emerging evidence that long-chain saturated fatty acid (SFA) might be implicated in ferroptosis, it remains unclear whether and how SFA participates in the process of ferroptosis. Using endogenous metabolites and genome-wide CRISPR screening, we have identified FAR1 as a critical factor for SFA-mediated ferroptosis. FAR1 catalyzes the reduction of C16 or C18 saturated fatty acid to fatty alcohol, which is required for the synthesis of alkyl-ether lipids and plasmalogens. Inactivation of FAR1 diminishes SFA-dependent ferroptosis. Furthermore, FAR1-mediated ferroptosis is dependent on peroxisome-driven ether phospholipid biosynthesis. Strikingly, TMEM189, a newly identified gene which introduces vinyl-ether double bond into alkyl-ether lipids to generate plasmalogens abrogates FAR1-alkyl-ether lipids axis induced ferroptosis. Our study reveals a new FAR1-ether lipids-TMEM189 axis dependent ferroptosis pathway and suggests TMEM189 as a promising druggable target for anticancer therapy.
Due to its high information density, DNA is very attractive as a data storage system. However, a major obstacle is the high cost and long turnaround time for retrieving DNA data with next‐generation ...sequencing. Herein, the use of a microfluidic very large‐scale integration (mVLSI) platform is described to perform highly parallel and rapid readout of data stored in DNA. Additionally, it is demonstrated that multi‐state data encoded in DNA can be deciphered with on‐chip melt‐curve analysis, thereby further increasing the data content that can be analyzed. The pairing of mVLSI network architecture with exquisitely specific DNA recognition gives rise to a scalable platform for rapid DNA data reading.
A non‐sequencing approach is demonstrated to perform high‐throughput retrieval of information encoded within DNA. The concept makes use of the hybridization of fluorescent DNA probes to the information stored within DNA sequence to perform rapid readout in a microfluidic very large‐scale integration (mVLSI) chip.
The Majorana search is caught up in an extensive debate about the false-positive signals from nontopological Andreev bound states. We introduce a remedy using the dissipative probe to generate ...electron-boson interaction. We theoretically show that the interaction-induced renormalization leads to significantly distinct universal zero-bias conductance behaviors, i.e., distinct characteristic power law in temperature, for different types of Andreev reflections, that show a sharp contrast to that of a Majorana zero mode. Various specific cases have been studied, including the cases in which two charges involved in an Andreev reflection process maintain or lose coherence, and the cases for multiple Andreev bound states with or without a Majorana. A transparent list of conductance features in each case is provided to help distinguish the observed subgap states in experiments, which also promotes the identification of Majorana zero modes.
It is well known that magnetic field is one of the effective tools to improve the activity of hydrogen evolution reaction (HER), but considering the inconvenient application of an external magnetic ...field, it is essential to find a ferromagnetic material with high HER activity itself. Fortunately, recent study has shown that the two‐dimmention (2D) Fe2Sn monolayer is a stable ferromagnetic topological Weyl semimetal material with high Tc of 433 K. Here, we report the Fe2Sn monolayer can be used as an alternative HER catalyst compared with expensive platinum (Pt). Our first‐principles results show that the Gibbs free energy (ΔGH*) value of the spin polarized Fe2Sn monolayer is −0.06 eV, much better than that without considering spin polarization (−1.23 eV). Moreover, the kinetic analysis demonstrates that the HER occurs on the Fe2Sn monolayer according to the Volmer‐Tafel mechanism with low energy barriers. Hence, our findings provide obvious evidence for spin‐polarization‐improved HER activity, paving a new way to design high‐performance HER catalysts.
Spin polarization: We investigate the hydrogen evolution reaction activity (HER) activity of basal plane of the 2D ferromagnetic Weyl semimetal Fe2Sn. Our findings show that the Fe2Sn monolayer in spin polarized state exhibit superior HER performance when compared with that in spin un‐polarized, indicating that spin polarization enables high HER activity.
Type 2 diabetes mellitus is now a worldwide health problem with increasing prevalence. Mounting efforts have been made to treat, prevent and predict this chronic disease. In recent years, increasing ...evidence from mice and clinical studies suggests that bone‐derived molecules modulate glucose metabolism. This review aims to summarize our current understanding of the interplay between bone and glucose metabolism and to highlight potential new means of therapeutic intervention. The first molecule recognized as a link between bone and glucose metabolism is osteocalcin (OCN), which functions in its active form, that is, undercarboxylated OCN (ucOC). ucOC acts in promoting insulin expression and secretion, facilitating insulin sensitivity, and favouring glucose and fatty acid uptake and utilization. A second bone‐derived molecule, lipocalin2, functions in suppressing appetite in mice through its action on the hypothalamus. Osteocytes, the most abundant cells in bone matrix, are suggested to act on the browning of white adipose tissue and energy expenditure through secretion of bone morphogenetic protein 7 and sclerostin. The involvement of bone resorption in glucose homeostasis has also been examined. However, there is evidence indicating the implication of the receptor activator of nuclear factor κ‐B ligand, neuropeptide Y, and other known and unidentified bone‐derived factors that function in glucose homeostasis. We summarize recent advances and the rationale for treating, preventing and predicting diabetes by skeleton intervention.
Pooled nucleic acid amplification test is a promising strategy to reduce cost and resources for screening large populations for infectious disease. However, the benefit of pooled testing is reversed ...when disease prevalence is high, because of the need to retest each sample to identify infected individual when a pool is positive. Split, Amplify, and Melt analysis of Pooled Assay (SAMPA) is presented, a multicolor digital melting PCR assay in nanoliter chambers that simultaneously identify infected individuals and quantify their viral loads in a single round of pooled testing. This is achieved by early sample tagging with unique barcodes and pooling, followed by single molecule barcode identification in a digital PCR platform using a highly multiplexed melt curve analysis strategy. The feasibility is demonstrated of SAMPA for quantitative unmixing and variant identification from pools of eight synthetic DNA and RNA samples corresponding to the N1 gene, as well as from heat-inactivated SARS-CoV-2 virus. Single round pooled testing of barcoded samples with SAMPA can be a valuable tool for rapid and scalable population testing of infectious disease.
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