To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) pretreatment on expression of myocardial heat shock protein (HSP) in acute myocardial ischemia-reperfusion injury (MIRI) ...rabbits.
A total of 72 New Zealand rabbits were randomly divided into 4 groups:sham operation, MIRI model, EA pretreatment and Moxi pretreatment (
=18 rabbits in each group) which were further divided into 0, 24 and 48 h (time-point) subgroups (
=6 in each). The MIRI model was established by occlusion of the anterior descending branch (ADB) of the left coronary artery for 40 min and reperfusion for 60 min. EA and Moxi stimulation was respectively applied to bilateral "Neiguan"(PC 6) for 20 min, once daily for 5 days before ADB occlusion. The expressions of myocardial HSP 27, HSP 70 and HSP 90 were detected by immunohistochemistry. The pathological and ultrastructural changes of left ventricular ischemia tissue were observed under light and transmission electronic microscope (TEM), respectively.
Outcomes of H.E. staining
A full-duplex optical passive access scheme is proposed and verified by simulation, in which hybrid 64/16/4-quadrature amplitude modulation（64/16/4QAM） orthogonal frequency division ...multiplexing（OFDM） optical signal is for downstream transmission and non-return-to-zero（NRZ） optical signal is for upstream transmission. In view of the transmitting and receiving process for downlink optical signal, in-phase/quadrature-phase（I/Q） modulation based on Mach-Zehnder modulator（MZM） and homodyne coherent detection technology are employed, respectively. The simulation results show that the bit error ratio（BER） less than hardware decision forward error correction（HD-FEC） threshold is successfully obtained over transmission path with 20-km-long standard single mode fiber（SSMF） for hybrid downlink modulation OFDM optical signal. In addition, by dividing the system bandwidth into several subchannels consisting of some continuous subcarriers, it is convenient for users to select different channels depending on requirements of communication.
The member of the kinesin-14 subfamily, KIFC1, is a carboxyl-terminal motor protein that plays an important role in the elongation of nucleus and acrosome biogenesis during the spermiogenesis of ...mammals. Here, we had cloned and sequenced the cDNA of a mammalian KIFC1 homologue (termed
ec
-KIFC1) from the total RNA of the testis of the reptile
Eumeces chinensis
. The full-length sequence was 2,339 bp that contained a 216 bp 5′-untranslated region (5′UTR), a 194 bp 3′-untranslated region (3′UTR) and a 1,929 bp open reading frame that encoded a special protein of 643 amino acids (aa). The calculated molecular weight of the putative
ec
-KIFC1 was 71 kDa and its estimated isoelectric point was 9.47. The putative
ec
-KIFC1 protein owns a tail domain from 1 to 116 aa, a stalk domain from 117 to 291 aa and a conserved carboxyl motor domain from 292 to 642 aa. Protein alignment demonstrated that
ec
-KIFC1 had 45.6, 42.8, 44.6, 36.9, 43.7, 46.4, 45.1, 55.6 and 49.8 % identity with its homologues in
Mus musculus
,
Salmo salar
,
Danio rerio
,
Eriocheir sinensis
,
Rattus norvegicus
,
Homo sapiens
,
Bos taurus
,
Gallus gallus
and
Xenopus laevis
, respectively. Tissue expression analysis showed the presence of ovary, heart, liver, intestine, oviduct, testis and muscle. The phylogenetic tree revealed that
ec
-KIFC1 was more closely related to vertebrate KIFC1 than to invertebrate KIFC1. In situ hybridization showed that the
ec
-KIFC1 mRNA was localized in the periphery of the nuclear membrane and the center of the nucleus in early spermatids. In mid spermatids, the
ec
-
KIFC1
had abundant expression in the center of nucleus, and was expressed in the tail and the anterior part of spermatids. In the late spermatid, the nucleus gradually became elongated, and the
ec
-KIFC1 mRNA signal was still centralized in the nucleus. In mature spermatids, the signal of the
ec
-KIFC1 gradually became weak, and was mainly located at the tail of spermatids. Therefore, the
ec
-KIFC1 probably plays a critical role in the spermatogenesis of
E. chinensis
.
KIF3A, the subunit within the kinesin-2 superfamily, is a typically N-terminal motor protein, which is involved in membranous organelle and intraflagellar transport. During spermatogenesis, KIF3A ...plays a critical role in the formation of flagella and cilia. KIF3A is also related to the left–right asymmetry, the signal pathway, DNA damage and tumorigenesis. We used RT-PCR and in situ hybridization to clone the
kif3a
gene, and we identified its function in the testis of the Chinese fire-bellied newt
Cynops orientalis
(termed as
co
-
kif3a
). The full-length sequence of
co
-
kif3a
was 2193 bp, containing a 56 bp 5′UTR, 2073 bp ORF encoding a protein of 691 amino acids and a 64 bp 3′UTR. The secondary structure analysis showed that co-KIF3A had three motor domains, representing the N-terminal motor domain (1–400 aa), α-helix domain (400–600 aa) and C-terminal tail domain (600–691 aa). The amino acid sequence of co-KIF3A shared an identity of 55.9%, 90.9%, 89.9%, 91.3% and 85.7% with its counterparts in
Aedes aegypti, Mus musculus, Xenopus tropicalis
,
Homo sapiens
and
Danio rerio
, respectively. The calculated molecular weight of the putative co-KIF3A was 79 kDa and its estimated isoelectric point was 6.8. RT-PCR result showed that
co
-
kif3a
was expressed in several examined tissues, with a high level in the testis and low levels in liver, muscle and ovum.
Kif3a
was weakly expressed in the heart and spleen, and barely detected in the intestine. In situ hybridization analysis demonstrated that in early spermatid
co
-
kif3a
was expressed around the nuclear membrane. When the tail began to emerge in the middle spermatid, mRNA transcript was abundantly concentrated in the flagellum. The mRNA signal was still very strong along all the flagellum in late spermatid. In mature spermatid, the message was weak. Therefore, co-KIF3A probably plays a functional role in the spermiogenesis of
C. orientalis
.
Abstract Breast cancer is one of the most common malignancies among gynecological diseases in the world and the long-term prognosis for breast cancer patients still remains dismal due to lack of ...effective early diagnosis biomarkers. Identifying sensitive and specific biomarkers in carcinogenesis may improve diagnostic and therapeutic strategies for this malignancy. Herein, we show that the expression of miR-1301 was markedly upregulated in breast cancer cell lines and tissues, and upregulation of miR-1301 enhanced, whereas downregulation of miR-1301 inhibited the proliferation of breast cancer cells in vitro . Furthermore, by biological approaches, we showed that miR-1301 directly targeted and suppressed ICAT expression, an important modulator of Wnt/β-Catenin pathway. These data suggests that miR-1301 may represent a novel therapeutic target of microRNA-mediated cell proliferation in breast cancer.
To describe an efficient and effective method of using Olympus TURis button plasma vaporization electrode plus loop electrode for transurethral vapor enucleation and resection of prostate.
Between ...July 2011 and October 2011, the investigators performed transurethral vapor enucleation and resection of prostate using Olympus TURis button plasma vaporization electrode plus loop electrode in 16 consecutive patients at our institution. The parameters of prostate weight, International Prostate Symptom Score (IPSS), quality of life (QOL), operative duration, blood loss volume, catheterization period, duration of hospitalization, perioperative complications and the weight of enucleated tissue were evaluated. IPSS and QOL were recorded during the follow-up.
No patient had significant blood loss or signs of transurethral resection syndrome. The mean patient age was 67.3 ± 8.1 years. Mean preoperative prostate weight was 49 ± 24 g (range: 19 - 91) and mean resected tissue weight 36 ± 16 g (range: 10 - 50). Serious compl
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