In this paper, we study the Hawking temperature of the BTZ black hole based on the purely topological method proposed by Robson, Villari, and Biancalana (RVB) Phys. Rev. D 99, 044042 (2019). The ...Hawking temperature of the charged rotating BTZ black hole can be accurately derived by this topological method. We also calculate the Hawking temperature of the BTZ black hole in massive gravity. Because the metric function of the BTZ black hole in massive gravity has a mass term, the corresponding Hawking temperature cannot be derived unless an integral constant is added.
Because of the intrinsic importance of nucleic acids as biotargets, the simple and sensitive detection of nucleic acids is very essential for biological studies and medical diagnostics. In this work, ...a novel, simple, and selective electrochemical DNA biosensor for the sensitive detection of target DNA (T-DNA) has been developed based on the dual-signaling electrochemical ratiometric method and exonuclease III (Exo III)-assisted target recycling amplification strategy. The assay strategy includes both “signal-on” and “signal-off” elements. The stem-loop (hairpin) DNA capture probe (HP), which was labeled by thiolated methylene blue (MB) at the 3′-protruding termini and ferrocene (Fc) in the middle of the loop, first self-assembled on the gold electrode surface via a Au–S bond. In the presence of T-DNA, the T-DNA hybridized with HP, which triggered the Exo III cleavage process and accompanied the release of T-DNA. As a result, the MB tags were away from and the Fc tags close to the gold electrode surface, leading to the decrease of the oxidation peak current of MB (I MB) and the increase of that of Fc (I Fc). The value of ΔI Fc/|ΔI MB| (ΔI Fc and ΔI MB are the change values of the oxidation peak currents of Fc and MB, respectively) is linear with the concentration of T-DNA from 0.01 pM to 0.8 pM. The detection limit (4.16 fM) of the developed method is much lower than that of the most reported electrochemical DNA biosensors. This strategy provides a simple and sensitive approach for the detection of T-DNA and has promising applications in bioanalysis, disease diagnostics, and clinical biomedicine.
Abstract
The detection of cyclotron resonance scattering features (CRSFs) is the only way to directly and reliably measure the magnetic field near the surface of a neutron star (NS). The broad energy ...coverage and large collection area of Insight-HXMT in the hard X-ray band allowed us to detect the CRSF with the highest energy known to date, reaching about 146 keV during the 2017 outburst of the first galactic pulsing ultraluminous X-ray source (pULX) Swift J0243.6+6124. During this outburst, the CRSF was only prominent close to the peak luminosity of ∼2 × 10
39
erg s
−1
, the highest to date in any of the Galactic pulsars. The CRSF is most significant in the spin-phase region corresponding to the main pulse of the pulse profile, and its centroid energy evolves with phase from 120 to 146 keV. We identify this feature as the fundamental CRSF because no spectral feature exists at 60–70 keV. This is the first unambiguous detection of an electron CRSF from an ULX. We also estimate a surface magnetic field of ∼1.6 × 10
13
G for Swift J0243.6+6124. Considering that the dipole magnetic field strengths, inferred from several independent estimates of magnetosphere radius, are at least an order of magnitude lower than our measurement, we argue that the detection of the highest-energy CRSF reported here unambiguously proves the presence of multipole field components close to the surface of the neutron star. Such a scenario has previously been suggested for several pulsating ULXs, including Swift J0243.6+6124, and our result represents the first direct confirmation of this scenario.
Coronavirus disease is an emerging infection caused by a novel coronavirus that is moving rapidly. High resolution computed tomography (CT) allows objective evaluation of the lung lesions, thus ...enabling us to better understand the pathogenesis of the disease. With serial CT examinations, the occurrence, development, and prognosis of the disease can be better understood. The imaging can be sorted into four phases: early phase, progressive phase, severe phase, and dissipative phase. The CT appearance of each phase and temporal progression of the imaging findings are demonstrated.
Background Several risk stratification models have been developed for cardiac surgery. This study aimed to evaluate the accuracy of four existing risk stratification models, the Fuwai System for ...Cardiac Operative Risk Evaluation （FuwaiSCORE）, the Society of Thoracic Surgeons 2008 cardiac surgery risk model for isolated valve surgery （the STS model）, the European System for Cardiac Operative Risk Evaluation （EuroSCORE） and the initial Parsonnet＇s score （the Parsonnet model） in predicting prolonged intensive care unit （ICU） stay in Chinese patients undergoing heart valve surgery. Methods Data were collected retrospectively from records of 1333 consecutive patients who received heart valve surgery in a single center between November 2006 and December 2007. Prolonged ICU stay was defined as not less than 124 hours. Calibration was assessed using the Hosmer-Lemeshow （H-L） goodness of fit test. Discrimination was assessed using the receiver-operating-characteristic （ROC） curve area. Results The FuwaiSCORE showed good calibration and discrimination compared with other risk models. According to the H-L statistics, the value of the FuwaiSCORE was 12.82, P 〉0.1. The area under ROC curve of the FuwaiSCORE was 0.81 （95%C/0.78-0.84）. Conclusions Our study suggests that the FuwaiSCORE is superior to the other three risk models in predicting prolonged length of ICU stay in Chinese patients with heart valve surgery. Having fewer variables, the system is much easier for bedside use than other systems.
Photodetection with extreme performances in terms of ultrafast response time, broad detection wavelength range, and high sensitivity has a wide range of optoelectronic and photonic applications, such ...as optical communications, interconnects, imaging, and remote sensing. Graphene, a typical two-dimensional Dirac semimetal, has shown excellent potential toward a high-performance photodetector with high operation speed, broadband response, and efficient carrier multiplications benefiting from its linear dispersion band structure with a high carrier mobility and zero bandgap. As the three-dimensional analogues of graphene, Dirac semimetal Cd3As2 processes all advantages of graphene as a photosensitive material but potentially has stronger interaction with light as a bulk material and thus enhanced responsivity. In this work, we report the realization of an ultrafast broadband photodetector based on Cd3As2. The prototype metal–Cd3As2–metal photodetector exhibits a responsivity of 5.9 mA/W with a response time of about 6.9 ps without any special device optimization. Broadband responses from 532 nm to 10.6 μm are achieved with a potential detection range extendable to far-infrared and terahertz. Systematical studies indicate that the photothermoelectric effect plays an important role in photocurrent generation. Our results suggest this emerging class of exotic quantum materials can be harnessed for photodetection with a high sensitivity and high speed (∼145 GHz) over a broad wavelength range.
Neuroinflammation, especially innate immunocyte-mediated neuroinflammation, has been reported to participate in pathogenesis of Alzheimer's disease (AD). However, the involvement of adaptive immune ...cells, such as CD4(+) T lymphocytes, in pathogenesis of AD is not well clarified. Herein, we focus on T helper 17 (Th17) cells, a subpopulation of CD4(+) T cells with high proinflammation, and show the implication of the cells in neurodegeneration of AD. Amyloid β1-42 (Aβ1-42) was bilaterally injected into hippocampus of rats to induce AD. On days 7 and 14 following the Aβ1-42 administration, escape latency of the rats in Morris water maze was increased, expression of amyloid precursor protein was upregulated, but expression of protein phosphatase 2A was downregulated in the hippocampus, and Nissl stain showed neuronal loss and gliosis in CA1 region. Infusion of FITC-linked albumin in blood circulation and combination with immunostaining of hippocampal sections for RORγ, a specific transcriptional factor of Th17 cells, demonstrated blood-brain barrier (BBB) disruption and Th17 cells' infiltration into brain parenchyma of AD rats. Expression of Th17 proinflammatory cytokines, interleukin (IL)-17 and IL-22, was increased in the hippocampus, and concentrations of the two cytokines were elevated in both the cerebrospinal fluid and the serum in AD occurrence and development. Compared with intact or saline-treated control rats, AD animals indicated an upregulated expression of Fas and FasL in the hippocampus. Further, the immunofluorescent histochemistry on AD hippocampal sections with NeuN, RORγ, Fas and FasL displayed that Fas was principally expressed by neurons and FasL was predominantly expressed by Th17 cells, and that neuronal apoptosis shown by TUNEL and NeuN double-labeled cells increased. These results suggest that Th17 cells, which were infiltrated into AD brain parenchyma, participate in neuroinflammation and neurodegeneration of AD by release of proinflammatory cytokines and by direct action on neurons via Fas/FasL apoptotic pathway.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Electro-Mechanical Actuator (EMA) is a key component in the flight control actuation of the more electric aircraft. The degradation of EMA has a significant influence on its performance. Hence, the ...degradation estimation of EMA needs to be studied, which is an important part of the health monitoring of EMA. However, the multiple failure modes of EMA bring much difficulty for implementing degradation estimation. To solve this problem, a fusion degradation estimation method based on Continuous Hidden Markov Model (CHMM) and Generalized Linear Model (GLM) (i.e., Multiple CHMM-GLM) is proposed for EMA with multiple failure modes. Firstly, Health Indicator (HI) extraction models are formulated based on CHMM for multiple failure modes to extract HIs, which can be used to indicate the degradation degree of EMA. Secondly, the fusion degradation model for EMA with multiple failure modes is formulated based on the extracted HIs and GLM, in which the nonlinearity of a single HI and the coupling among multiple HIs are both considered. Finally, the output of the fusion degradation model is regarded as a HI to indicate the degradation degree of EMA. This study provides a new way for the degradation estimation of EMA with multiple failure modes, which can contribute to the Prognostics and Health Management (PHM) development of EMA. To evaluate the effectiveness of the proposed method, experiments are conducted utilizing EMA simulation and practical data. Experimental results show that the proposed method has a good performance in the degradation estimation for EMA with multiple failure modes.
The aryl‐to‐vinyl nickel 1,4‐migration (1,4‐Ni migration) reaction has been reported for the first time. The generated alkenyl Ni species undergo a reductive coupling reaction with unactivated ...brominated alkanes affording a series of trisubstituted olefins. This tandem reaction exhibits mild conditions, a broad substrate scope, high regioselectivity, and excellent Z/E stereoselectivity. A series of controlled experiments have shown that the critical 1,4‐Ni migration process is reversible. In addition, the alkenyl nickel intermediates obtained after migration are highly Z/E stereoselective and do not undergo Z/E isomerization. The obtained trace isomerization products are caused by the instability of the product.
The 1,4‐nickel migration process from aryl to alkenyl groups is now reported for the first time. The alkenyl nickel intermediates generated by this highly stereoselective migration process can be coupled with brominated alkanes to form multisubstituted olefins. The current method provides a new and efficient approach for the synthesis of Z/E olefins with high stereoselectivity.
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