Glycopeptides of hMOG(30–50) containing a glucosyl moiety on the side-chains of Asn, Ser or Hyp at position 31 were synthesised. Antibody titres to hMOG(30–50) and to its glucoderivatives were ...measured by ELISA in sera of patients affected by different neurological diseases. Anti-hMOG(30–50) antibodies were detected only using the glycopeptide Asn
31(
N-Glc)hMOG(30–50).
The glycopeptide Asn
31(
N-Glc)hMOG(30–50) is the first specific antigen able to identify anti-hMOG(30–50) antibodies hMOG(30–50), human myelin oligodendrocyte glycoprotein 30–50 in sera of patients affected by different neurological diseases. On the contrary, Ser
31(
O-Glc)hMOG(30–50) and Hyp
31(
O-Glc)hMOG(30–50) did not allow the antibody recognition.
O6-Butylguanine was detected in the urine of rats given the butylating agent N-nitroso-N-butylurea. O6-Butylguanine contents in the 24-h rat urine samples after i.p. doses of 50, 100, and 200 mg/kg ...N-nitroso-N-butylurea were 1.03 +/- 0.41 (SE), 8.30 +/- 1.70, and 59.53 +/- 6.52 pmol, respectively. This suggests that O6-butylguanine formation in nucleic acids might be repaired in vivo, possibly by base excision, besides other mechanisms. After i.v. doses of 0.1 and 1 mg/kg of O6-butylguanine to rats urinary excretion did not exceed 2% of the administered dose, suggesting that the amount of O6-butylguanine effectively released by base excision might be much larger than that detected in the urine after N-nitroso-N-butylurea. Inhibition of the enzyme O6-alkyl-DNA transferase by N-nitrosodimethylamine increased urinary O6-butylguanine resulting from exposure to N-nitroso-N-butylurea (100 mg/kg i.p.) up to four times, thus confirming an alternative DNA repair mechanism.
A convenient one-pot methodology for the preparation of 3-alkoxy-2-bromopropanoic esters from alkyl acrylates is described. The use of stoichiometric amounts of alkyl acrylate, alcohol and mercury ...trifluoroacetate in THF makes this procedure versatile and attractive to achieve building blocks for the synthesis of targeted contrast agents for Magnetic Resonance Imaging.
GIANO is an IR cross-dispersed echelle spectrometer designed to achieve high throughput, high resolving power, wide band coverage and high accuracy radial velocity measurements. It also includes ...polarimetric capabilities and a low resolution mode that make it a very versatile, common user instrument which will be permanently mounted and available at one of the Nasmyth foci of the Telescopio Nazionale Galileo (TNG) located at Roque de Los Muchachos Observatory (ORM), La Palma, Spain. This project was selected by INAF as the top priority instrument among those proposed within the Second Generation Instrumentation Plan of the TNG. More information on this project can be found at the web page http://www.bo.astro.it/giano.
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