To investigate the role of FOXM1, β-catenin and TCF4 in esophageal cancer (EC) and their relationship to VM (Vasculogenic Mimicry).
CCK-8 were performed to examine EC cell proliferation in FOXM1 ...silenced cells. EC cell migration and invasion were investigated through wound healing and Transwell assays, respectively. The formation of pipe like structures were assessed in 3D cultures. The expression of Foxm1, β-catenin, Tcf4 and E-cadherin were investigated through western blot, RT-qPCR and immunohistochemistry (IHC) staining. The relationship between FOXM1 expression, clinic-pathological features, and overall survival (OS) were further analyzed.
A loss of FOXM1 expression correlated with the OS of ESCC patients. FOXM1 silencing led to a loss of cell growth and suppressed cell migration and invasion in ESCC cells. VM structures were identified in ESCC tissues and human EC cell lines. Mechanistically, FOXM1 was found to promote tumorigenesis through the regulation of β-catenin, Tcf4, and E-cadherin in EC cells, leading to the formation of VM structures.
These findings highlight FoxM1 as a novel therapeutic target in ESCC.
Objective
Symptoms such as depression and anxiety are common psychiatric symptoms in patients with chronic obstructive pulmonary disease (COPD). Cognitive behavioral therapy (CBT) is still ...controversial in the treatment of anxiety and depression in patients with COPD. We conducted a meta‐analysis and systematic review to evaluate the effect of CBT on anxiety and depression in patients with COPD, with a view to providing some guidance for clinical application.
Materials and methods
Computer search Web of Science, EMbase, PubMed, Cochrane Library, search time limit from the establishment of the library to August 2019. Collect the randomized controlled trial (RCT) for this topic. Two investigators independently screened the literature according to inclusion and exclusion criteria, extracted the data and assessed the risk of bias in the included studies. Meta‐analysis using RevMan5.3 software.
Results
A total of 10 studies were included in a total of 1278 patients. Meta‐analysis shows that CBT can improve depression and anxiety in patients with COPD. Subgroup analysis showed that intervention time ≥8 weeks had significant differences in improving anxiety, while intervention time <8weeks had significant differences in improving depression.
Conclusions
Cognitive behavioral therapy may possibly relieve depression in COPD patients in a short period of time, and it takes longer to improve anxiety. Therefore, clinical practice can choose the appropriate intervention time according to the patient’s psychological condition.
Ideological and political course is the main carrier of ideological and political education for students. This paper firstly introduces the theoretical basis and practical basis of the research-based ...(guidance-inquiry-based) teaching model, and then analyses the new opportunities and challenges for the ideological and political education at the Internet era. Finally, it elaborates the application of guidance-inquiry-based teaching model in the ideological and political education of colleges and universities at the Internet age.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Objective To investigate the role of FOXM1, beta-catenin and TCF4 in esophageal cancer (EC) and their relationship to VM (Vasculogenic Mimicry). Methods CCK-8 were performed to examine EC cell ...proliferation in FOXM1 silenced cells. EC cell migration and invasion were investigated through wound healing and Transwell assays, respectively. The formation of pipe like structures were assessed in 3D cultures. The expression of Foxm1, beta-catenin, Tcf4 and E-cadherin were investigated through western blot, RT-qPCR and immunohistochemistry (IHC) staining. The relationship between FOXM1 expression, clinic-pathological features, and overall survival (OS) were further analyzed. Results A loss of FOXM1 expression correlated with the OS of ESCC patients. FOXM1 silencing led to a loss of cell growth and suppressed cell migration and invasion in ESCC cells. VM structures were identified in ESCC tissues and human EC cell lines. Mechanistically, FOXM1 was found to promote tumorigenesis through the regulation of beta-catenin, Tcf4, and E-cadherin in EC cells, leading to the formation of VM structures. Conclusions These findings highlight FoxM1 as a novel therapeutic target in ESCC. Keywords: Esophageal cancer, FOXM1, beta-Catenin, Tcf4, VM, Proliferation, Invasion, Migration
The C/Si switch strategy has been regarded as a useful and efficient strategy for the discovery of drugs and materials. Thus, development of a methodology to access diverse silacycles is of great ...significance and in great demand. Among these, C–H bond silylation provides a powerful and straightforward synthetic method to form diverse silacycles in an atom- and step-economical fashion. However, C–H bond silylation has not been used to access any six-membered silicon-bridged π-conjugated scaffolds and enantioselective six-membered C–H silylation has never been presented. Herein, we successfully accessed diverse six-membered π-conjugated dibenzooxasilines via C–H bond silylation and investigated their photophysical properties. Furthermore, we realized enantioselective six-membered C–H siylation to directly afford planar chiral metallocene oxasilolanes with high ee (up to 95% ee). We also demonstrated the synthetic usefulness of dibenzooxasilines and planar chiral metallocene-fused benzooxasilolines as valuable synthetic intermediates via diverse additional transformations. Moreover, six-membered silicon-bridged ladder π-conjugated systems were designed and rapidly constructed by using our methods. The “isomerization” and “silicon” effects on molecular geometries and photophysical properties were also evaluated in detail.
The synthesis of lignin-based graphene quantum dots (GQDs) with excellent fluorescence stability, quantum yield, and biocompatibility for sensitive and selective detection of Fe3+ and ascorbic acid ...(AA) has remained a challenging endeavor. Using an acidolysis process with 17.5% nitric acid followed by hydrothermal treatment at 200 °C, this study provided an improved synthesis route for the production of high-quality GQDs from alkali lignin. The nitrogen-doped GQDs exhibit remarkable fluorescence stability under a wide range of pH (3–10), duration (1–12 h), and NaCl (0–1000 mM) conditions, and have a high quantum yield of 28%. The GQDs or GQDs/Fe3+ sensing systems (GQDs at 50 mg L−1, Fe3+ at 500 μmol L−1, and UV excitation at 370 nm) for fluorescence sensing of Fe3+ or AA have excellent sensitivity, selectivity, and reproducibility. For Fe3+ and AA, the limit of detection is 1.49 and 1.62 μmol L−1, respectively. Mechanism investigation shows that photoluminescence quenching is caused by the formation of GQDs-Fe3+ complexes, whereas fluorescence recovery is due to Fe3+ reduction by AA.
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•An improved route for GQDs synthesis from alkali lignin is developed.•The GQDs have excellent photostability and a high quantum yield of 28%.•The GQDs fluorescence probes have an LOD of 1.49 and 1.62 μM for Fe3+ and AA.•Fluorescence quenching is caused by the formation of GQDs-Fe3+ complexes.•The reduction of Fe3+ by AA is the underlying mechanism of fluorescence recovery.
Glioblastoma multiforme (GBM) is among the most aggressive of human cancers. Although differentiation therapy has been proposed as a potential approach to treat GBM, the mechanisms of induced ...differentiation remain poorly defined. Here, we established an induced differentiation model of GBM using cAMP activators that specifically directed GBM differentiation into astroglia. Transcriptomic and proteomic analyses revealed that oxidative phosphorylation and mitochondrial biogenesis are involved in induced differentiation of GBM. Dibutyryl cyclic AMP (dbcAMP) reverses the Warburg effect, as evidenced by increased oxygen consumption and reduced lactate production. Mitochondrial biogenesis induced by activation of the CREB-PGC1α pathway triggers metabolic shift and differentiation. Blocking mitochondrial biogenesis using mdivi1 or by silencing PGC1α abrogates differentiation; conversely, overexpression of PGC1α elicits differentiation. In GBM xenograft models and patient-derived GBM samples, cAMP activators also induce tumor growth inhibition and differentiation. Our data show that mitochondrial biogenesis and metabolic switch to oxidative phosphorylation drive the differentiation of tumor cells.
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•The anti-Warburg effect is elicited by cAMP activation in GBM cells•Metabolic switch to oxidative phosphorylation drives the differentiation of GBM•Mitochondrial biogenesis by cAMP activation triggers metabolic reprogramming•The cAMP-CREB-PGC1α signal induces mitochondrial biogenesis and metabolic shift
Xing et al. show that the metabolic shift from glycolysis to oxidative phosphorylation drives differentiation of GBM cells into astrocytes by cAMP activation. Mechanistically, the cAMP-CREB-PGC1α signal mediates mitochondrial biogenesis, which leads to metabolic reprogramming, induced differentiation, and tumor growth inhibition.
In this paper, the finite-time (FNT) cluster synchronization of complex dynamic networks (CDNs) is discussed on the basis of encoding and decoding communication protocol involved in network attacks. ...Compared with general CDNs, the proposed discrete-time CDNs with denial-of-service (DoS) attacks and bit-rate constraints is considered, which is more consistent with the actual digital communication network. First, a complete encoding and decoding step based on the uniform quantizer is introduced. And more importantly, DoS attacks, whose duration and frequency duration are described by average dwell-time constraints, are considered in such steps, which clearly analyzes attacks influence on codeword string. In order to avoid encoding and decoding failure caused by unbounded encoding region, the attack is limited to a certain range, and the sufficient conditions for bounded encoding region are obtained. Second, in order to overcome the uncertainty of errors between encoded data and decoded data, sufficient conditions for decoding error convergence are obtained, which makes it possible to analyze the FNT cluster synchronization of CDNs based on the encoding decoding communication mechanism. Third, by Lyapunov stability theory, the synchronization controller is obtained to ensure that the nodes in the cluster are consistent, and the relationship between the upper bound of decoding error (UBDE) and the available bit rate is revealed. Finally, a simulation example shows that the bit-rate allocation protocol can reduce the UBDE and the time required for synchronization.
Purpose
Laparoscopic nephrectomy (LN) has become the preferred method for renal cell carcinoma (RCC). Adequate preoperative assessment or intraoperative navigation is key to the successful ...implementation of LN. The aim of this study was to evaluate the clinical application value of mixed‐reality–assisted surgical navigation (MRASN) in LN.
Patients and Methods
A total of 100 patients with stage T1N0M0 renal tumors who underwent laparoscopic partial nephrectomy (LPN) or laparoscopic radical nephrectomy (LRN) were prospectively enrolled and divided into a mixed‐reality‐assisted laparoscopic nephrectomy (MRALN) group (n = 50) and a non–mixed‐reality‐assisted laparoscopic nephrectomy (non‐MRALN) group (n = 50). All patients underwent renal contrast‐enhanced CT scans. The CT DICOM data of all patients in the MRALN group were imported into the mixed‐reality (MR) postprocessing workstation and underwent holographic three‐dimensional visualization (V3D) modeling and MR displayed, respectively. We adopted the Likert scale to evaluate the clinical application value of MRASN. The consistency of evaluators was assessed using the Cohen kappa coefficient (k).
Results
No significant differences in patient demographic indicators between the MRALN group and the non‐MRALN group (P > .05). The subjective score of MRASN clinical application value in operative plan formulation, intraoperative navigation, remote consultation, teaching guidance, and doctor‐patient communication were higher in the MRASN group than in the non‐MRASN group (all P < .001). There were significantly more patients for whom LPN was successfully implemented in the MRALN group than in the non‐MRALN group (82% vs 46%, P < .001). The MRALN group had a shorter operative time (OT) and warm ischemia time (WIT) and less estimated blood loss (EBL) than the non‐MRALN group (all P < .001).
Conclusion
MRASN is helpful for operative plan formulation, intraoperative navigation, remote consultation, teaching guidance, and doctor‐patient communication. MRALN may effectively improve the successful implementation rate of LPN and reduce the OT, WIT, and EBL.
MRASN is helpful for operative plan formulation, intraoperative navigation, remote consultation, teaching guidance, and doctor‐patient communication. MRALN may effectively improve the successful implementation rate of LPN and reduce the OT, WIT, and EBL.
The oxyhalide photo-thermo-refractive (PTR) glass has found various applications in optical devices. However, due to its complex compositions, researches on the structures and structure-property ...relationship of this kind of materials is still limited. In this work, we applied molecular dynamics (MD) simulations to investigate the structure of the classic PTR glass (excluding Ce, Ag, Sb, and Sn). A set of bromine-related potential parameters has been developed and tested by comparing the simulated crystal structures with those from experiments or ab initio calculations. The PTR glass has then been simulated by using MD simulations with the newly developed potentials. The structure information of the PTR glass, such as the pair distribution function, bond angle distribution, coordination number, and two-dimensional distributions of elements, have been calculated and compared with available experimental data from literature. In addition, the growth of the fluorine/bromine-rich regions in the PTR glass after heat treatment has also been investigated. It is found that the bromine prefers to stay around the fluorine-rich regions and form the phase boundary between the fluorine-rich phase and the oxygen-rich glass matrix. And the presence of sodium ions in the fluorine/bromine-rich regions increase the aggregation tendency of these regions thus lead to the growth of the region size. The results show that the newly developed bromine/fluorine-related potential parameters can describe the PTR glass structures thus provide a new method to help design and improve new applications.
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